Growth Kinetic Study of Blue-green Microalgae Arthrospira platensis Using Buffalo Manure as Alternative Media

2021 ◽  
Vol 14 (4) ◽  
pp. 5
Author(s):  
Merry Martgrita ◽  
Brian Sinaga ◽  
Lianty Simangunsong ◽  
Andy Trirakhmadi ◽  
Monita Pasaribu
Keyword(s):  
Growth Kinetic ◽  
Kinetic Modelling ◽  
Arthrospira Platensis ◽  
Alternative Media ◽  
Growth Media ◽  
Monod Model ◽  
Sample Analysis ◽  
Growth Data ◽  
High Accumulation ◽  
North Sumatra

North Sumatera is one of the provinces in Indonesia with the highest buffalo population, which is responsible for the high accumulation of buffalo manure that can cause environmental and aesthetic problems if left untreated. One of the possible alternatives for solving this issue is by implementing buffalo manure as growth media for microorganisms, e.g. microalgae. In this research, buffalo manure was used as alternative media for Arthospira platensis cultivation. Buffalo manure was taken from Sitoluama village, Laguboti, Toba Regency of North Sumatra Province. Research steps included media and culture preparation, cultivation, sampling, sample analysis and verification of constructed models and validation. Buffalo manure concentration in media is varied from 1 g.L-1 to 8 g.L-1 which is analogous to nitrogen content of 0.002 mg.L-1 to 0.018 mg.L-1. Growth data was used for growth kinetic modelling, which was most satisfactory for Monod model (µmax = 0.5915 day-1, Ks = 0.421 g.L-1).

scholarly journals ANALISIS VIABILITAS LACTOBACILLUS LACTIS PADA INOVASI MEDIA DASAR PERTUMBUHAN ALTERNATIF DAN MEDIA DASAR PENEPUNGAN BAKTERI ASAM LAKTAT

2020 ◽  
Vol 4 (2) ◽  
pp. 16-22
Author(s):  
Ani Sulastri ◽  
Baso Manguntungi
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Shelf Life ◽  
Bacterial Growth ◽  
Alternative Media ◽  
Plate Count ◽  
Growth Media ◽  
Media Formulation ◽  
The Media ◽  
Lactobacillus Lactis

The limited shelf life in a food requires a natural preservative so that the food used is not easily damaged and has a longer shelf life, namely by using lactic acid bacteria (BAL) using alternative media. By using lactic acid bacteria, the time in the storage period food products can be extended. The purpose of this study was to determine the viability of the Lactobacillus lactis bacteria on an alternative growth base media and a media on the media of bacteria. Lactic acid bacteria were rejuvenated and culture propagation of 5 ?l was inoculated into 5 mL of MRSB media. Formulation media used for bacterial growth such as whey tofu + 5% sucrose + 1% urea. The alternative media was incubated for 24 hours. Bacterial growth was observed at 0, 4, 8 and 16 hours using the TPC (Total Plate count) method. Various media Lactobacillus lactis bacterial deposition was grown on MRSB media and dried with freeze dry for 48 hours and the viability of Lactobacillus lactis was tested. The basic growth media that can be used are Lactobacillus lactis bacteria, namely whey tofu + sucrose 5% + urea 1% as well as Lactobacillus lactis viability results in various media which are grown on MRS media and various alternative media shows that the media has a 100% carrageenan composition able to maintain the viability of Lactobacillus lactis cells.

scholarly journals Optimisation and growth kinetic analysis of Microalgae, Arthrospira platensis in 2-L Photobioreactors

2021 ◽  
Vol 842 (1) ◽  
pp. 012036
Author(s):  
A A Hussin ◽  
S W To ◽  
M H Sani ◽  
M F M Amin ◽  
M F Kamaroddin
Keyword(s):  
Kinetic Analysis ◽  
Growth Kinetic ◽  
Arthrospira Platensis

scholarly journals Rapid Accumulation of Total Lipid inRhizoclonium africanumKutzing as Biodiesel Feedstock under Nutrient Limitations and the Associated Changes at Cellular Level

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Gour Gopal Satpati ◽  
Sanjit Kanjilal ◽  
Rachapudi Badari Narayana Prasad ◽  
Ruma Pal
Keyword(s):  
Fatty Acids ◽  
Total Lipid ◽  
Structural Changes ◽  
Neutral Lipids ◽  
Lipid Production ◽  
Biodiesel Production ◽  
Phosphate Limitation ◽  
Growth Media ◽  
Electron Microscopic ◽  
High Accumulation

Increase of total lipid and the proportion of the favorable fatty acids in marine green filamentous macroalgaRhizoclonium africanum(Chlorophyceae) was studied under nitrate and phosphate limitations. These stresses were given by both eliminating and doubling the required amounts of nitrate and phosphate salts in the growth media. A significant twofold increase in total lipid (193.03 mg/g) was achieved in cells in absence of nitrate in the culture medium, followed by phosphate limitation (142.65 mg/g). The intracellular accumulation of neutral lipids was observed by fluorescence microscopy. The scanning electron microscopic study showed the major structural changes under nutrient starvation. Fourier transform infrared spectroscopy (FTIR) revealed the presence of ester (C-O-C stretching), ketone (C-C stretching), carboxylic acid (O-H bending), phosphine (P-H stretching), aromatic (C-H stretching and bending), and alcohol (O-H stretching and bending) groups in the treated cells indicating the high accumulation of lipid hydrocarbons in the treated cells. Elevated levels of fatty acids favorable for biodiesel production, that is, C16:0, C16:1, C18:1, and C20:1, were identified under nitrate- and phosphate-deficient conditions. This study shows that the manipulation of cultural conditions could affect the biosynthetic pathways leading to increased lipid production while increasing the proportion of fatty acids suitable for biodiesel production.

scholarly journals Ulitization of Oil Palm Empty Bunches Waste as a Growth Media for Straw Mushrooms

2021 ◽  
Vol 6 (1) ◽  
pp. 168-173
Author(s):  
Liana Dwi Sri Hastuti ◽  
Erman Munir ◽  
Rendra Syahputra Tanjung ◽  
Iradani Yupita Ningrum
Keyword(s):  
Palm Oil ◽  
Oil Palm ◽  
Nutritional Value ◽  
Growth Media ◽  
Mushroom Cultivation ◽  
Oil Processing ◽  
Straw Mushroom ◽  
Marketing Activities ◽  
Market Needs ◽  
North Sumatra

Straw Mushroom (Volvariella volvaceae) as well asmushrooms edible otherhave been developed in Indonesia, including North Sumatra as a substitute for animal protein. Mushrooms or also known as button mushrooms are vegetables that are recognized to have high nutritional value, and are even believed to have medicinal properties for various types of diseases so that they have a fairly high selling value. Some studies report that in 100 grams of mushroom, only 0.17% fat is contained and even better, this fat is not bad fat. Seeing the high demand because of its nutritional value, and its rich content, its taste that is liked by many people, mushroom cultivation has a very promising market value. Basically until now the demand for mushroom continues to increase, but farmers have not been able to meet market needs. good quality start to be limited. Some farmers have started to switch to alternative planting media which is currently mostly practiced, namely oil palm pulp or empty palm oil bunches (TKKS). EFB is a waste that is very easy and is found mostly around palm oil processing factories, whose use has been limited so far as heating materials for boilers and particle wood, many empty bunches have not been utilized. This service aims to provide training in the form of mushroom cultivation using TKKS, as well as provide training in the form of post-harvest handling which is important given the lack of understanding of farmers in production and marketing activities.

scholarly journals The Isolation of Staphylococcus Epidermidis Bacteria in White Snapper Salted Fish (Lates Calcalifer) of Sibolga City, North Sumatera Province

2020 ◽  
Vol 7 (1) ◽  
pp. 44
Author(s):  
Rafika Lestari ◽  
Masda Admi ◽  
Rastina Rastina ◽  
Maryulia Dewi ◽  
Nurliana Nurliana ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Staphylococcus Epidermidis ◽  
Bacterial Contamination ◽  
Agar Plate ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Growth Media ◽  
Salted Fish ◽  
Gram Staining ◽  
North Sumatra

Salted fish is vulnerable to contamination by microbial. Staphylococcus epidermidis is one of the bacteria that can contaminate the salted fish. This research aims to isolate the Staphylococcus epidermidis bacteria contamination in salted white snapper fish sold in Sibolga City, North Sumatra Province. The sample used was white snapper salted fish, amounting to 10 samples from 10 traders. The isolation of Staphylococcus epidermidis was carried out using the Carter method. White snapper salted fish are mashed using a blender, then planted on Nutrient Broth (NB) as a bacterial growth media. Furthermore, identification of bacterial colonies grew using gram staining, Manitol Salt Agar (MSA) media, Blood Agar Plate (BAP) media, catalase test and confectionery media (Manitol and Glucose). The data obtained were analyzed descriptively. The results of this study suggest that Staphylococcus epidermidis contains bacterial contamination. Based on the data collected, it can be concluded that 7 out of 10 samples of white snapper salted fish sold in Sibolga City, North Sumatra Province, are contaminated with 70 percent Staphylococcus epidermidis bacteria.

Assessing Functional Language in School-Aged Children Using Language Sample Analysis

2020 ◽  
Vol 5 (3) ◽  
pp. 622-636
Author(s):  
John Heilmann ◽  
Alexander Tucci ◽  
Elena Plante ◽  
Jon F. Miller
Keyword(s):  
Computer Hardware ◽  
School Age Children ◽  
Functional Language ◽  
School Age ◽  
Clinical Use ◽  
Sample Analysis ◽  
Validity And Reliability ◽  
Language Sample Analysis ◽  
Accuracy And Repeatability ◽  
Language Sample

Purpose The goal of this clinical focus article is to illustrate how speech-language pathologists can document the functional language of school-age children using language sample analysis (LSA). Advances in computer hardware and software are detailed making LSA more accessible for clinical use. Method This clinical focus article illustrates how documenting school-age student's communicative functioning is central to comprehensive assessment and how using LSA can meet multiple needs within this assessment. LSA can document students' meaningful participation in their daily life through assessment of their language used during everyday tasks. The many advances in computerized LSA are detailed with a primary focus on the Systematic Analysis of Language Transcripts (Miller & Iglesias, 2019). The LSA process is reviewed detailing the steps necessary for computers to calculate word, morpheme, utterance, and discourse features of functional language. Conclusion These advances in computer technology and software development have made LSA clinically feasible through standardized elicitation and transcription methods that improve accuracy and repeatability. In addition to improved accuracy, validity, and reliability of LSA, databases of typical speakers to document status and automated report writing more than justify the time required. Software now provides many innovations that make LSA simpler and more accessible for clinical use. Supplemental Material https://doi.org/10.23641/asha.12456719

Issues in Language Sample Collection and Analysis With Children Using AAC

2014 ◽  
Vol 23 (2) ◽  
pp. 65-74 ◽  
Author(s):  
Gail Van Tatenhove
Keyword(s):  
Clinical Practice ◽  
Augmentative And Alternative Communication ◽  
Language Production ◽  
Expressive Language ◽  
Sample Analysis ◽  
Alternative Communication ◽  
Sample Collection ◽  
Speech Language Pathologists ◽  
Language Sample Analysis ◽  
Language Sample

Language sample analysis is considered one of the best methods of evaluating expressive language production in speaking children. However, the practice of language sample collection and analysis is complicated for speech-language pathologists working with children who use augmentative and alternative communication (AAC) devices. This article identifies six issues regarding use of language sample collection and analysis in clinical practice with children who use AAC devices. The purpose of this article is to encourage speech-language pathologists practicing in the area of AAC to utilize language sample collection and analysis as part of ongoing AAC assessment.

Evaluation of Azadirachtin on Arthrospira plantensis Gomont growth parameters and antioxidant enzymes

2020 ◽  
Vol 56 ◽  
pp. 8
Author(s):  
Hatice Tunca ◽  
Ali Doğru ◽  
Feray Köçkar ◽  
Burçin Önem ◽  
Tuğba Ongun Sevindik
Keyword(s):  
Enzyme Activity ◽  
Growth Parameters ◽  
Arthrospira Platensis ◽  
Proline Content ◽  
Enzyme Analysis ◽  
Free Proline ◽  
Sod Activity ◽  
Pesticide Pollution ◽  
Antioxidant Parameters ◽  
Mda Content

Azadirachtin (Aza) used as insecticide due to inhibiting growth of insects and preventing them from feeding on plants. To understand the effects of contamination of this insecticide on phototrophs, and to determine the responses of these organisms against these insecticides are extremely important in understanding how the ecosystem is affected. In this study, chlorophyll-a amount, OD 560 and antioxidant parameters (total SOD, APX, GR, Proline, MDA and H2O2) were determined in order to understand the effect of Aza on Arthrospira platensis Gomont. Aza was applied between 0–20 μg mL−1 concentrations for 7 days in the study. Enzyme analysis was conducted at the end of the 7th day. There was a statistically significant decrease in the absorbance of OD560 and the chlorophyll-a content in A. platensis cultures exposed to the Aza (0–20 μg mL−1) during 7 days due to the increase in pesticide levels. SOD activity decreased at 8, 16 and 20 μg mL−1 concentrations; GR enzyme activity showed a significant decrease compared to the control at a concentration of 20 μg mL−1. APX activity did not change significantly compared to control. The MDA content increased significantly at 16 and 20 μg mL−1 concentrations. The H2O2 content significantly increased at 12, 16 and 20 μg mL−1 concentrations (p < 0.05) while the free proline content decreased at 4 μg mL−1 concentration (p < 0.05). As a result, regarding the Aza concentrations used in this study may be a step to prevent pesticide pollution in the environment.

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