scholarly journals The Isolation of Staphylococcus Epidermidis Bacteria in White Snapper Salted Fish (Lates Calcalifer) of Sibolga City, North Sumatera Province

2020 ◽  
Vol 7 (1) ◽  
pp. 44
Author(s):  
Rafika Lestari ◽  
Masda Admi ◽  
Rastina Rastina ◽  
Maryulia Dewi ◽  
Nurliana Nurliana ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Staphylococcus Epidermidis ◽  
Bacterial Contamination ◽  
Agar Plate ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Growth Media ◽  
Salted Fish ◽  
Gram Staining ◽  
North Sumatra

Salted fish is vulnerable to contamination by microbial. Staphylococcus epidermidis is one of the bacteria that can contaminate the salted fish. This research aims to isolate the Staphylococcus epidermidis bacteria contamination in salted white snapper fish sold in Sibolga City, North Sumatra Province. The sample used was white snapper salted fish, amounting to 10 samples from 10 traders. The isolation of Staphylococcus epidermidis was carried out using the Carter method. White snapper salted fish are mashed using a blender, then planted on Nutrient Broth (NB) as a bacterial growth media. Furthermore, identification of bacterial colonies grew using gram staining, Manitol Salt Agar (MSA) media, Blood Agar Plate (BAP) media, catalase test and confectionery media (Manitol and Glucose). The data obtained were analyzed descriptively. The results of this study suggest that Staphylococcus epidermidis contains bacterial contamination. Based on the data collected, it can be concluded that 7 out of 10 samples of white snapper salted fish sold in Sibolga City, North Sumatra Province, are contaminated with 70 percent Staphylococcus epidermidis bacteria.

scholarly journals Isolasi dan identifikasi Staphylococcus Epidermidis pada susu sapi PFH penderita mastitis subklinis di Wukirsari, Cangkringan, Sleman, DIY

2018 ◽  
Vol 28 (2) ◽  
pp. 121
Author(s):  
Ervina Ryan Puspasari ◽  
Yanuartono - Yanuartono ◽  
Sri Hartati ◽  
Slamet Rahardjo ◽  
Alfarisa Nururrozi ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Management Practices ◽  
Agar Plate ◽  
Brain Heart Infusion ◽  
Subclinical Mastitis ◽  
High Rate ◽  
Blood Agar Plate ◽  
Milk Samples ◽  
Gram Staining ◽  
High Incidence

Mastitis is an inflammation of the mammary gland, can be occured clinically or subclinically. One of the causes of mastitis is <em>Staphylococcus epidermidis</em>. This study was aimed to isolate and identify <em>Staphylococcus epidermidis</em> in subclinical mastitis PFH dairy cows in Wukirsari, Cangkringan, Sleman, DIY. Sixteen milk samples were tested with a Californian Mastitis Test (CMT), followed by inoculation in Brain Heart Infusion (BHI), planted on a blood agar plate plate (PAD) and Mac Conkey Agar (MCA) for 24 hours at 37<sup>0</sup>C. The growing colonies were then grown on sugar sugar and nutrient agar (NA) media. Gram staining, catalase test, coagulase test and DNase test as bacteria confirmation.CMT test results showed 15 (93.75%) out of 16 positive milk samples suffered from subclinical mastitis. Results of isolation showed 1 (6.66%)of 16 positive samples infected with Staphylococcus epidermidis.The results of this study indicate that incidence of subclinical mastitis in the study area showed a high rate and is likely to be caused by inadequate management practices and milking procedures. The high incidence of subclinical mastitis is probably caused by other bacteria because only 6.66% is caused by <em>Staphylococcus epidermidis</em>.

scholarly journals Diagnostic Performance of Various Methodologies for Group B Streptococcus Screening in Pregnant Woman in China

2021 ◽  
Vol 11 ◽  
Author(s):  
Kankan Gao ◽  
Qiulian Deng ◽  
Lianfen Huang ◽  
Chien-Yi Chang ◽  
Huamin Zhong ◽  
...  
Keyword(s):  
Group B Streptococcus ◽  
Agar Plate ◽  
Reference Method ◽  
Antigen Detection ◽  
The Other ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Chromogenic Agar ◽  
Group B ◽  
Detection Reagent

Maternal vaginal/rectal colonization of group B streptococcus (GBS) is a main risk for neonatal invasive infection. Efficient determination of GBS colonization in pregnant women is crucial. This study aimed to investigate the prevalence of GBS carriage and evaluate the diagnostic performance of six methodologies for GBS screening conducted in China, including blood agar plate, liquid chromogenic medium, and loop-mediated isothermal amplification (LAMP) without pre-enrichment, chromogenic agar plate with pre-enrichment, and GBS antigen detection without and with pre-enrichment in comparison with the standard reference method (Lim broth-enriched subculture with plating on 5% sheep blood agar). Vaginal/rectal swabs were collected from 1,281 pregnant women at 35–37 weeks of gestation. Of them, 309 were taken in triplicate, one for Lim broth-enriched subculture, one for blood agar plate, and the third for GBS antigen detection (Reagent W); 177 were acquired in duplicate, one for Lim broth-enriched subculture and the other for GBS antigen detection (Reagent H); 502 were obtained in duplicate, one for Lim broth-enriched subculture and the other for liquid chromogenic medium; 158 were collected in duplicate, one for Lim broth-enriched subculture and the other for LAMP; and 135 were inoculated in Lim broth-enriched for GBS antigen detection (Reagent W) and subculture with chromogenic agar plate and 5% blood agar plate. The overall prevalence of GBS carriage was 10.1% (130/1,281, 95% CI: 8.5–12.1%) according to the standard reference method. Compared with the standard reference method, the LAMP had excellent performance of sensitivity (100%, 95%CI: 83.4–100%), specificity (94%, 95%CI: 88.1–97.1%), and Yoden index (0.940); as well as the blood agar plate with sensitivity (81.5%, 95%CI: 61.3–93.0%), specificity (100%, 95%CI: 98.3–100.0%), and Yoden index (0.815). The other four methods were not sufficient to reach the threshold in terms of sensitivity or specificity compared to the standard reference method. Furthermore, for LAMP, results can be obtained within 0.5–1 h, while for blood agar plate, which needed 24–48 h, and further identification was required. Our data suggested that the performance of LAMP was highly comparable to the standard Lim broth-enriched subculture and LAMP is considered as an alternative for fast and accurate GBS screening.

scholarly journals Comparative Evaluation of Efficacy of Chlorhexidine and Herbal Mouthwash as a Preprocedural Rinse in Reducing Dental Aerosols: A Microbiological Study

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Sangeeta Umesh Nayak ◽  
Anushka Kumari ◽  
Valliammai Rajendran ◽  
Vijendra Pal Singh ◽  
Ashwini Hegde ◽  
...  
Keyword(s):  
Agar Plate ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Study Patient ◽  
Microbial Culture ◽  
Mouth Rinse ◽  
Mouth Rinses ◽  
Intergroup Comparison ◽  
Viable Bacteria ◽  
Post Hoc

Objective. The risk to dentists, assistants, and patients of infectious diseases through aerosols has long been recognized. The aim of this study was to evaluate and compare the efficacy of commercially available preprocedural mouth rinses containing 0.2% chlorhexidine (CHX) gluconate, Befresh™ herbal mouthwash, and water in reducing the levels of viable bacteria in aerosols. Materials & Methods. This was a single-center, double-masked, placebo-controlled, randomized, three-group parallel design study. 30 patients (10 patients in each group) were recruited in the study. Patient rinsed mouth with 10 ml of CHX, 10 ml Befresh™, or 10 ml water. All the patients underwent supragingival ultrasonic scaling for a minimum of 30 min. The aerosol collection was done using a blood agar plate. The blood agar plates were kept approximately 12 inches from the patient’s mouth. The microbial culture was analyzed. The colony-forming unit (CFU) counting in all three groups was assessed using one-way ANOVA test to compare among the groups (p value <0.001). The intergroup comparison was done using the post hoc Tukey test. Result. There was a marked reduction in the CFU in the CHX group in all three areas. This was followed by Befresh™ (Sagar Pharmaceuticals) mouthwash. There was no reduction in the CFU of the water group. Conclusion. This study proves that a regular preprocedural mouth rinse could significantly reduce the majority bacteria present in aerosols generated by the use of an ultrasonic unit, and Befresh™ mouth rinse was found to be equally effective in reducing the aerosol contamination to 0.2% CHX gluconate.

scholarly journals Sterility Duration of Preprimed Extracorporeal Membrane Oxygenation Circuits

2018 ◽  
Vol 23 (4) ◽  
pp. 311-314 ◽  
Author(s):  
Vi Ean Tan ◽  
Alan T. Evangelista ◽  
Dominick M. Carella ◽  
Daniel Marino ◽  
Wayne S. Moore ◽  
...  
Keyword(s):  
Extracorporeal Membrane Oxygenation ◽  
Room Temperature ◽  
Agar Plate ◽  
Fungal Growth ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Macconkey Agar ◽  
Pilot Data ◽  
Sheep Blood ◽  
Membrane Oxygenation

OBJECTIVES There is a lack of standardization and supporting data regarding the duration preassembled and preprimed extracorporeal membrane oxygenation (ECMO) circuits are expected to be sterile. Therefore, the purpose of this study was to prospectively evaluate whether preassembled and preprimed ECMO circuits could maintain sterility for a period up to 65 days. DESIGN Four ECMO circuits (2 neonatal/pediatric¼” and 2 adolescent/adult ⅜ ”) were assembled and primed under sterile conditions and maintained at room temperature. Culture samples were obtained from each circuit and plated within 1 hour. Culture samples were obtained on day 0 when assembled and primed then every 5 days up to day 65. Samples were plated on several different media including the following: blood agar plate: trypticase soy agar with 5% sheep blood, MacConkey agar, and thioglycollate broth then incubated at 35°C for 3 days. RESULTS All cultures obtained from the priming solution from of the¼” and ⅜ ” ECMO circuits produced no microbial or fungal growth for the 65-day study period. CONCLUSION These pilot data suggest preprimed ECMO circuits may maintain sterility for a period up to 65 days. Additional studies evaluating a larger number of ECMO circuits are needed to confirm these findings.

scholarly journals Improved reliability of the primary plate bacitracin test on throat cultures with sulfamethoxazole-trimethoprim blood agar plates

1979 ◽  
Vol 9 (1) ◽  
pp. 144-146 ◽  
Author(s):  
T Kurzynski ◽  
C Meise ◽  
R Daggs ◽  
A Helstad
Keyword(s):  
Agar Plate ◽  
Susceptibility Test ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Group A Streptococci ◽  
Sheep Blood Agar Plate ◽  
Group A ◽  
Primary Plate ◽  
Disk Susceptibility

The primary plate bacitracin differentiation disk susceptibility test identified 85% of group A streptococci from throat cultures on SXT-BA(CO2) plates within 24 h, as compared to only 26% on a conventional sheep blood agar plate.

scholarly journals THE BLOOD-AGAR PLATE FOR SPORE-FORMING ANAËROBES

1926 ◽  
Vol 11 (5) ◽  
pp. 305-321
Author(s):  
Luther Thompson
Keyword(s):  
Agar Plate ◽  
Blood Agar ◽  
Blood Agar Plate
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