Investigation of metal exudates from tobacco glandular trichomes under heavy metal stresses using a variable pressure scanning electron microscopy system

Use of variable Pressure SEMs is spreading among electron microscopists The variable Pressure SEM does not necessarily require specimen Preparation such as fixation, dehydration, coating, etc which have been required for conventional scanning electron microscopy. The variable Pressure SEM allows operating Pressure of 1˜270 Pa in specimen chamber It does not allow microscopy of water-containing specimens under a saturated vapor Pressure of water. Therefore, it may cause shrink or deformation of water-containing soft specimens such as plant cells due to evaporation of water. A solution to this Problem is to lower the specimen temperature and maintain saturated vapor Pressures of water at low as shown in Fig. 1 On this technique, there is a Published report of experiment to have sufficient signal to noise ratio for scondary electron imaging at a relatively long working distance using an environmental SEM. We report here a new low temperature microscopy of soft Plant cells using a variable Pressure SEM (Hitachi S-225ON).

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Microscopy Methods for Biofilm Imaging: Focus on SEM and VP-SEM Pros and Cons

Biology ◽

10.3390/biology10010051 ◽

2021 ◽

Vol 10 (1) ◽

pp. 51

Author(s):

Michela Relucenti ◽

Giuseppe Familiari ◽

Orlando Donfrancesco ◽

Maurizio Taurino ◽

Xiaobo Li ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Ion Beam ◽

Ruthenium Red ◽

Variable Pressure ◽

Sem Images ◽

Advantages And Disadvantages ◽

Pros And Cons ◽

Microscopy Techniques ◽

Scanning Electron

Several imaging methodologies have been used in biofilm studies, contributing to deepening the knowledge on their structure. This review illustrates the most widely used microscopy techniques in biofilm investigations, focusing on traditional and innovative scanning electron microscopy techniques such as scanning electron microscopy (SEM), variable pressure SEM (VP-SEM), environmental SEM (ESEM), and the more recent ambiental SEM (ASEM), ending with the cutting edge Cryo-SEM and focused ion beam SEM (FIB SEM), highlighting the pros and cons of several methods with particular emphasis on conventional SEM and VP-SEM. As each technique has its own advantages and disadvantages, the choice of the most appropriate method must be done carefully, based on the specific aim of the study. The evaluation of the drug effects on biofilm requires imaging methods that show the most detailed ultrastructural features of the biofilm. In this kind of research, the use of scanning electron microscopy with customized protocols such as osmium tetroxide (OsO4), ruthenium red (RR), tannic acid (TA) staining, and ionic liquid (IL) treatment is unrivalled for its image quality, magnification, resolution, minimal sample loss, and actual sample structure preservation. The combined use of innovative SEM protocols and 3-D image analysis software will allow for quantitative data from SEM images to be extracted; in this way, data from images of samples that have undergone different antibiofilm treatments can be compared.

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Scanning Electron Microscopy Coupled with Energy Dispersive Spectrometric Analysis Reveals for the First Time Weddellite and Sylvite Crystals on the Surface of Involucral Bracts and Petals of two Xeranthemum L. (Compositae) Species

Microscopy and Microanalysis ◽

10.1017/s1431927617000551 ◽

2017 ◽

Vol 23 (3) ◽

pp. 679-686 ◽

Cited By ~ 2

Author(s):

Milan Gavrilović ◽

Suzana Erić ◽

Petar D. Marin ◽

Núria Garcia-Jacas ◽

Alfonso Susanna ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Glandular Trichomes ◽

Energy Dispersive ◽

Spectrometric Analysis ◽

Eds Analysis ◽

Taxonomic Studies ◽

First Time ◽

Tetragonal Bipyramid ◽

Scanning Electron

AbstractIn this work, weddellite and sylvite crystals are identified for the first time on the involucral bracts and petals of Xeranthemum annuum and Xeranthemum cylindraceum using scanning electron microscopy coupled with energy dispersive spectrometric (SEM-EDS) analysis. Well-developed crystals of weddellite (CaC2O4·2H2O) occur in the form of a tetragonal bipyramid (hhl), rarely in combination of a bipyramid and tetragonal prism (h00). Indumentum of involucral bracts of X. cylindraceum consists of nonglandular and glandular trichomes. Sylvite (KCl) crystals are observed only on the petal surface of X. cylindraceum. The crystals of sylvite occur in the form of perfect cubes (hexahedrons), but some crystals are deformed, i.e., partially elongated. Taxonomic significance of investigated microcharacters as well as the use of SEM-EDS analysis in taxonomic studies of plants are discussed.

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Petal and Sepal Epidermal Micromorphology of Six Lathyrus Taxa (Fabaceae) and their Systematic Value

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4017503 ◽

2012 ◽

Vol 40 (1) ◽

pp. 35 ◽

Cited By ~ 8

Author(s):

HÃ¼seyin CILDIR ◽

Ahmet KAHRAMAN ◽

Musa DOGAN

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Glandular Trichomes ◽

Systematic Significance ◽

Trichome Types ◽

First Time ◽

Scanning Electron

In this study, the epidermal types and their distribution on dorsal and lateral petals, the trichome types and their density on sepals of Lathyrus chloranthus, L. digitatus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, L. sativus, and L. tuberosus, belonging to sections Lathyrus, Lathyrostylis, Pratensis, and Orobon of the genus Lathyrus in Turkey were investigated using light microscopy (LM) and scanning electron microscopy (SEM), and the systematic significance of these characters was evaluated. These taxa, except L. sativus, are studied for the first time under aspects of the petal and sepal micromorphology. Three major epidermal types were recognized on the petal: tabular rugose striate cells (TRS), areolate cells with more or less striations (AS), and papillose conical striate cells (PCS). TRS and AS were further subdivided into three subtypes. TRS was found on the dorsal and lateral petals of L. chloranthus, L. sativus, and L. tuberosus: the dorsal petals of L. roseus subsp. roseus and the lateral petals of L. laxiflorus subsp. laxiflorus, while AS was present on the dorsal and lateral petals of L. digitatus and the lateral petals of L. roseus subsp. roseus. PCS was found only on the dorsal petals of L. digitatus. Three main types of trichomes on the sepal were observed: peltate glandular, capitate glandular, and nonglandular trichomes. The capitate glandular and nonglandular trichomes were further subdivided into three subtypes. The peltate glandular trichomes were present only in L. chloranthus, but absent in the others. The capitate glandular trichomes were found in L. chloranthus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, and L. tuberosus. The nonglandular trichomes were always present in L. chloranthus and L. laxiflorus subsp. laxiflorus. The present results show that the petal and sepal micromorphology can be used in delimitation of the taxa based on petal and sepal micromorphology.

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Ambient Variable Pressure Field Emission Scanning Electron Microscopy for Trichome Profiling of Plectranthus tomentosa by Secondary Electron Imaging

Applied Microscopy ◽

10.9729/am.2013.43.1.34 ◽

2013 ◽

Vol 43 (1) ◽

pp. 34-39 ◽

Cited By ~ 4

Author(s):

Ki Woo Kim

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Field Emission ◽

Secondary Electron ◽

Pressure Field ◽

Variable Pressure ◽

Electron Imaging ◽

Scanning Electron

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Asbestos fiber identification in liver from cholangiocarcinoma patients living in an asbestos polluted area: a preliminary study

Tumori Journal ◽

10.1177/0300891619839305 ◽

2019 ◽

Vol 105 (5) ◽

pp. 404-410 ◽

Cited By ~ 2

Author(s):

Federica Grosso ◽

Alessandro Croce ◽

Roberta Libener ◽

Narciso Mariani ◽

Massimo Pastormerlo ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Asbestos Exposure ◽

Optical Microscope ◽

Variable Pressure ◽

Chrysotile Asbestos ◽

Neoplastic Tissue ◽

Asbestos Fibers ◽

Preliminary Study ◽

Scanning Electron

Purpose: To assess whether asbestos fibers may be observed in liver tissue of patients with cholangiocarcinoma (CC) with environmental or working asbestos exposure. Methods: Detection of fibers was performed directly on histologic sections of liver from 7 patients with CC using optical microscope and variable pressure scanning electron microscopy equipped with energy-dispersive spectroscopy (VP-SEM/EDS). All patients were from Casale Monferrato, Italy, a highly asbestos-polluted town. Due to ethical constraints, observers were blinded to patients’ clinical features. Results: Fibers/bundles of fibers of chrysotile were detected in 5 out of 7 patients (71%). The boundary between healthy and neoplastic tissue or the fibrocollagen tissue produced by the neoplasia were identified as areas of fiber incorporation. Conclusions: This study is the first report about the detection of chrysotile asbestos fibers in the liver of patients with CC. Further studies on larger cohorts are needed to corroborate our preliminary findings.

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