Phytochemical screening of various extracts of leaves of Stereospermum personatum (Hassk.) Chatterjee with a focus on GC-MS analysis of bioactive compounds in methanolic extract

2021 ◽  
Vol 11 (3) ◽  
pp. 2507-2511
Author(s):  
Indira Indira G ◽  
Padmaja Padmaja V
Keyword(s):  
Bioactive Compounds ◽  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Ms Analysis

scholarly journals GC-MS ANALYSIS AND EXTRACTION OPTIMIZATION OF BIOACTIVE COMPOUNDS FROM PELARGONIUM GRAVEOLENS LHER METHANOLIC EXTRACT AND THEIR ACTIVITIES AS PHARMACOLOGICAL AGENTS

2019 ◽  
Vol 10 (9) ◽  
pp. 59-72
Author(s):  
Daniel M Makanyane ◽  
Ikechukwu P Ejidike ◽  
Cornelius C Ssemakalu ◽  
Fanyana M Mtunzi ◽  
Vusumzi E Pakade ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Methanolic Extract ◽  
Pharmacological Agents ◽  
Pelargonium Graveolens ◽  
Extraction Optimization ◽  
Ms Analysis

scholarly journals Phytochemical screening and GC MS analysis of methanolic extract of Abelmoschus moschatus

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 3049-3052
Author(s):  
Rani Sebastian ◽  
Jayakar B ◽  
Gomathi V
Keyword(s):  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Cytoprotective Activity ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Acetate Methyl ◽  
Study Results ◽  
Gas Chromatography Mass Spectroscopy ◽  
Vinyl Phenol

The study was designed to evaluate the phytoconstituents present in the methanolic extract of aerial parts of Abelmoschus moschatus. The qualitative phytochemical screening of different extracts of aerial parts revealed the presence of some bioactive compounds. GC – MS analysis was performed using Shimadzu Gas chromatography-mass spectroscopy (Model Number: QP2010S) instrument. GC-MS detection of phytoconstituents was done by computer evaluation of mass spectra of samples through National Institute Standard and Technology (NIST II) and WILEY 8 library. GC – MS analysis detected the presence of 14 compounds. GC – MS profile of the methanolic extract revealed the presence of megastigmatrienone, phytol, loliolide, farnesyl acetate, methyl linoleates, gamma-sitosterol, cis, cis, cis-7,10,13-Hexadecatrienal, thymine, pyranone, coumarin, 2 – methoxy 4 – vinyl phenol, guanosine, chinasaure and 3- cyclopentyl propionic acid 2 dimethyl aminoethyl ester. The current study suggests that methanolic extracts of aerial parts of Abelmoschus moschatus contain phytoconstituents with antioxidant and cytoprotective activity. The study results will pave a way for the production of therapeutic agents which can be used for the treatment of various diseases.

Preliminary phytochemical screening and GC-MS analysis of methanolic extract of Turnera subulata Smith (Passifloraceae)

2017 ◽  
Vol 6 (3) ◽  
pp. 174-177
Author(s):  
◽  
S Vinotha ◽  
A Vijayan ◽  
Anand Gideon V ◽  
◽  
...  
Keyword(s):  
Leaf Extract ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Plant Leaf ◽  
Ms Analysis ◽  
Phytochemical Profile ◽  
Gas Chromatography Mass Spectroscopy ◽  
Plant Leaf Extract

The investigation was carried out to determine the possible phytochemical constituents from aqueous, methanol and chloroform extracts of Turnera subulata leaf extracts. Among the phytochemical screening of these extracts, Methanolic extract showed that the leaf was rich in alkaloids, flavonoids, glycosides, phenols, saponins and quinones. The chemical composition of the plant leaf extract of T. subulata was investigated using Gas Chromatography – Mass Spectroscopy (Agilent-7890A GC instrument coupled with MS-5975) and NIST-MS library. GC-MS analysis of T. subulata plant leaf extract, revealed the existence of the GC-MS chromatogram of the major peaks presented in methanolic extract like Methyl 8,11,14-heptadecatrienoate (23.244%), Pentadecanoic acid, 14-methyl-,methyl ester (8.654%), n-Hexadecanoic acid (8.654%), 4H-Pyran4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (6.598%), 1b,4a-Epoxy-2H-cyclopenta[3,4] cyclopropa[8,9]cycloundec[1,2-b] oxiren-5(1aH)-one(5.400%), 3,7,11,15-Tetramethyl-2-hexadecen-1- ol(5.400%), etc. From this study it is obvious that T. subulata leaf extract contains many biologically active compounds and also it gives a detailed insight about the phytochemical profile which could be exploited for the development of plant based drug.

scholarly journals Treating Hyperglycemia From Eryngium caeruleum M. Bieb: In-vitro α-Glucosidase, Antioxidant, in-vivo Antidiabetic and Molecular Docking-Based Approaches

2020 ◽  
Vol 8 ◽  
Author(s):  
Abdul Sadiq ◽  
Umer Rashid ◽  
Sadiq Ahmad ◽  
Mohammad Zahoor ◽  
Mohamed F. AlAjmi ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Free Radicals ◽  
Bioactive Compounds ◽  
Active Sites ◽  
Methanolic Extract ◽  
Array Detector ◽  
Chloroform Fraction ◽  
Ms Analysis

Natural-based drugs are believed to be safe, effective and economical. Based on the medicinal importance of the genus Eryngium and unexplored nature of Eryngium caeruleum, we have evaluated its antidiabetic and antioxidant potentials. Both in-vitro and in-vivo assays have been carried out for antidiabetic assays. The antioxidant activity was determined by using different free radicals [i.e., 1,1-diphenyl,2-picrylhydrazyl (DPPH), 2,2-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS), and hydrogen peroxide (H2O2)]. Moreover, different phytoconstituents were identified in the most active solvent fraction by GC-MS analysis. Furthermore, comparative fingerprints of methanolic extract and chloroform fraction were also analyzed via High Performance Liquid Chromatography coupled with Diode Array Detector (HPLC-DAD). The crude methanolic extract of E. caeruleum (Ec.Cr) and its sub-fractions [i.e., n-hexane (Ec.Hex), chloroform (Ec.Chf), ethyl acetate (Ec.EtAc), and aqueous (Ec.Aq) were employed in this study]. In the α-glucosidase inhibition assay, a concentration-dependent inhibitory response was observed against the enzyme. The most active sample was Ec.Chf which revealed an IC50 of 437 μg/ml in comparison to the standard acarbose (IC50 25 μg/ml). The rest of the samples showed moderate inhibition of α-glucosidase. In antioxidant assays, Ec.Chf and Ec.Cr exhibited a considerable scavenging effect against all the free radicals. The IC50 values recorded for Ec.Chf were 112, 109, and 150 μg/ml against DPPH, ABTS, and H2O2 respectively. Based on the in-vitro potential of Ec.Chf, this was subjected to the in-vivo model experiment. The Ec.Chf lowered the blood glucose level up to 10.3 mmol/L at 500 μg/Kg. The Ec.Chf was also subjected to GC-MS analysis. The GC-MS analysis confirmed the presence of 60 compounds. The identified phytoconstituents consist of some essential compounds previously reported with antidiabetic and antioxidant studies, which include thymol, tocopherol, phytol, nerolidol, (I)-neophytadiene, linolenic acid, and falcarinol. Similarly, the HPLC-DAD chromatograms of Ec.Cr and Ec.Chf exhibited a variety of peaks, which further demonstrates the possibility of important phytochemicals. In a nutshell, we can conclude that Eryngium caeruleum is a potential source of bioactive compounds which may be beneficial for the management of ailments like diabetes and free radicals mediated disorders. Molecular docking was performed to explore the possible role of all the identified bioactive compounds in the chloroform fraction of Eryngium caeruleum into active sites of the homology model of α-glucosidase.

scholarly journals Preliminary Phytochemical Screening and GC-MS Analysis of Methanolic Extract of Roots of Pandanus fascicularis

2022 ◽  
pp. 626-631
Author(s):  
Mary Sheeja TL ◽  
Jessy Jacob ◽  
Jaykar Balasundaram ◽  
Gomathi Venkatachalam
Keyword(s):  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Ms Analysis

scholarly journals PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF METHANOLIC EXTRACT OF BOMBAX CEIBA BARK

2018 ◽  
Vol 9 (4) ◽  
pp. 67-70
Author(s):  
Meenakshi S C ◽  
Basavaraj S Beldal ◽  
Ramesh L Londonkar
Keyword(s):  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Ms Analysis

The GC-MS analysis of methanolic extract of Chlorophytum borivilianum and compounds’ activities validation at standard databases

2022 ◽  
Vol 11 (6) ◽  
pp. 768-774
Author(s):  
Pankaj Kushwaha ◽  
Shashi Alok ◽  
Lavkush Dwivedi
Keyword(s):  
Methanolic Extract ◽  
Octadecadienoic Acid ◽  
Food Additives ◽  
The Novel ◽  
Phytochemical Screening ◽  
Chlorophytum Borivilianum ◽  
Palmitoyl Chloride ◽  
Ms Analysis ◽  
Medicinal Properties ◽  
Safed Musli

Chlorophytum borivilianum (Safed Musli), a member of the Liliaceae family is a well-known plant in India for its aphrodisiac and adaptogenic activities. The present study was carried out to identify the novel compounds based new medicinal properties of the plant. The preliminary phytochemical screening and GC-MS based chromatographic analysis of a methanolic extract of C. borivilianum (MECB) tubors resulted the detection of Phenol, Tannins, Flavo-noids, Alkaloids, Saponins, and Glycosides in the plant. In GC-MS analysis of the MECB, more than 200 compounds were identified in different peak are-as. All of them were crosschecked at various online compound databses like PubChem, Drugbank, etc. for the identification of their known biological ac-tivities, if any. Out of them, mainly 21 compounds (falling in 70.74% peak area) like 4-mercaptophenol, 1,4-Benzenediol, 2-methoxy-, Octadecanal, Pentadecanal, 9,12-Octadecadienoic acid, Betulin, Levomenthol, etc. were characterized in this study. As per their reported medicinal activities at standard databases the plant tubor is hereby reported to have anti-microbial, analgesic, anti-inflammatory, anti-neoplastic, anti-pruritic, anti-tussive and anti-spasmodic activites. Moreover, the compounds like Cyclo-hexanol, 5-methyl-2-(1-methylethyl), Palmitoyl Chloride, Triarachine, Phytol, 4-mercaptophenol with their mentholating, food additives and other valua-ble effects have underlined the industrial value of the plant.

scholarly journals Antioxidant Potential and GC-MS Analysis of Abrus precatorius Linn Leaves Ethanol Extract

2021 ◽  
pp. 39-46
Author(s):  
Bhimraj Gawade ◽  
Mazahar Farooqui
Keyword(s):  
Free Radical ◽  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Antioxidant Potential ◽  
Mass Spectrometry Analysis ◽  
Phytochemical Screening ◽  
Abrus Precatorius ◽  
Ms Analysis

Aims: The aim of present study to evaluate the antioxidant potential of Abrus precatorius Linn leaves ethanol extract containing bioactive compounds on free radical scavenger using DPPH. Ethanol extract of Abrus precatorius Linn leaves was subjected to preliminary phytochemical screening and fraction of extract was detected by Gas Chromatography-Mass Spectrometry analysis. Methodology and Results: GC-MS analysis of ethanol leaves extract was carried out on Shimadzu GC-MS model number QP 2010S and revealed the presence of 18 phytochemical compounds, with mome inositol as a dominant component. Antioxidant potential of leaves ethanol extract was evaluated using DPPH free radical scavenging assay at five different doses as 5, 10, 20, 30, 40 and 50 μg/mL and showed significant DPPH free radical scavenging potential with the IC50 value of 33.37 μg/mL. The bioautography of extract showed that fractions with the most prominent antioxidant potential tended to contain secondary metabolites reported in preliminary phytochemical screening such as alkaloids, carbohydrates, protein and amino acids, glycoside, tannins, flavonoids, triterpenoids and phenolic compounds. The results evaluate and justify the traditional relevance of Abrus precatorius Linn leaves ethanol extract for free radical scavenging potential as a antioxidant and can be used as a lead for the isolation of the antioxidant bioactive chemical constituents in further study. Conclusion: Preliminary phytochemical screening reported various bioactive compounds in the ethanol extract of Abrus precatorius Linn leaves and identified by GC-MS. The extract exhibited greater free radical scavenging activity i.e. antioxidant potential. The presence of various bioactive phytocompounds justifies the therapeutic use of the Abrus precatorius Linn plant leaves for various ailments by traditional practitioners.

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