scholarly journals Phytochemical screening and GC MS analysis of methanolic extract of Abelmoschus moschatus

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 3049-3052
Author(s):  
Rani Sebastian ◽  
Jayakar B ◽  
Gomathi V
Keyword(s):  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Cytoprotective Activity ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Acetate Methyl ◽  
Study Results ◽  
Gas Chromatography Mass Spectroscopy ◽  
Vinyl Phenol

The study was designed to evaluate the phytoconstituents present in the methanolic extract of aerial parts of Abelmoschus moschatus. The qualitative phytochemical screening of different extracts of aerial parts revealed the presence of some bioactive compounds. GC – MS analysis was performed using Shimadzu Gas chromatography-mass spectroscopy (Model Number: QP2010S) instrument. GC-MS detection of phytoconstituents was done by computer evaluation of mass spectra of samples through National Institute Standard and Technology (NIST II) and WILEY 8 library. GC – MS analysis detected the presence of 14 compounds. GC – MS profile of the methanolic extract revealed the presence of megastigmatrienone, phytol, loliolide, farnesyl acetate, methyl linoleates, gamma-sitosterol, cis, cis, cis-7,10,13-Hexadecatrienal, thymine, pyranone, coumarin, 2 – methoxy 4 – vinyl phenol, guanosine, chinasaure and 3- cyclopentyl propionic acid 2 dimethyl aminoethyl ester. The current study suggests that methanolic extracts of aerial parts of Abelmoschus moschatus contain phytoconstituents with antioxidant and cytoprotective activity. The study results will pave a way for the production of therapeutic agents which can be used for the treatment of various diseases.

Preliminary phytochemical screening and GC-MS analysis of methanolic extract of Turnera subulata Smith (Passifloraceae)

2017 ◽  
Vol 6 (3) ◽  
pp. 174-177
Author(s):  
◽  
S Vinotha ◽  
A Vijayan ◽  
Anand Gideon V ◽  
◽  
...  
Keyword(s):  
Leaf Extract ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Plant Leaf ◽  
Ms Analysis ◽  
Phytochemical Profile ◽  
Gas Chromatography Mass Spectroscopy ◽  
Plant Leaf Extract

The investigation was carried out to determine the possible phytochemical constituents from aqueous, methanol and chloroform extracts of Turnera subulata leaf extracts. Among the phytochemical screening of these extracts, Methanolic extract showed that the leaf was rich in alkaloids, flavonoids, glycosides, phenols, saponins and quinones. The chemical composition of the plant leaf extract of T. subulata was investigated using Gas Chromatography – Mass Spectroscopy (Agilent-7890A GC instrument coupled with MS-5975) and NIST-MS library. GC-MS analysis of T. subulata plant leaf extract, revealed the existence of the GC-MS chromatogram of the major peaks presented in methanolic extract like Methyl 8,11,14-heptadecatrienoate (23.244%), Pentadecanoic acid, 14-methyl-,methyl ester (8.654%), n-Hexadecanoic acid (8.654%), 4H-Pyran4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl (6.598%), 1b,4a-Epoxy-2H-cyclopenta[3,4] cyclopropa[8,9]cycloundec[1,2-b] oxiren-5(1aH)-one(5.400%), 3,7,11,15-Tetramethyl-2-hexadecen-1- ol(5.400%), etc. From this study it is obvious that T. subulata leaf extract contains many biologically active compounds and also it gives a detailed insight about the phytochemical profile which could be exploited for the development of plant based drug.

scholarly journals GC-MS Analysis, Phytochemical Screening and Biological Activity of Bauhinia tomentosa (Linn.)

2021 ◽  
Vol 33 (11) ◽  
pp. 2641-2644
Author(s):  
R. Mini ◽  
V. Prabhu ◽  
K. Vimaladevi ◽  
J. Sowmiya ◽  
G. Sibi
Keyword(s):  
Biological Activity ◽  
Methanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Zone Of Inhibition ◽  
Tetradecanoic Acid ◽  
Ms Analysis ◽  
Ic50 Value ◽  
Gas Chromatography Mass Spectroscopy ◽  
Minor Compounds

Present study aimed to evaluate the chemical composition and biological activity for methanolic extract of Bauhinia tomentosa (Linn.) leaves grown in Western Ghats region of South India. The preliminary phytochemical screening tests revealed the presence of steroids, alkaloids, terpenoids, flavonoids, glycosides and phenolic compounds in the leaf extract. A total of 19 compounds were identified through gas chromatography-mass spectroscopy (GC-MS) analysis of methanolic extract of B. tomentosa. The major compounds identified were phytol (23.96%), n-hexadecanoic acid (11.62%), squalene (8.85%) and the minor compounds are trans-bis(2-methylpropyl)-4,6-dioxane(0.13%), dihydro-cis-α-copaene-8-ol (0.14%), tetradecanoic acid (0.81%), respectively. Antibacterial activity of the extract showed the zone of inhibition 18 mm at 200 μg/mL against S. aureus, followed by 15 and 16 mm against S. anginosus, K. pneumoniae at 200 μg/mL, respectively. Antioxidant activity of methanolic extract of B. tomentosa leaves showed the maximum IC50 value with 75.07 % of scavenging activity at the concentration of 5 μg/mL.

scholarly journals Evaluation of the phytochemical profile and uterine contractile effect of Woodfordia fruticosa (L.) Kurz and Dipsacus mitis D. Don

2012 ◽  
Vol 26 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Rajendra Gyawali ◽  
Deepak Jnawali ◽  
Song Chang-Hun ◽  
Kyong-Su Kim
Keyword(s):  
Volatile Compounds ◽  
Steam Distillation ◽  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Rat Uterus ◽  
Methanolic Extracts ◽  
Uterine Contractile ◽  
Phytochemical Profile ◽  
Mass Spectrometery ◽  
Woodfordia Fruticosa

The phytochemical screening of Dipsacus mitis D. Don and Woodfordia fruticosa (L.) Kurz revealed the presence of bioactive secondary metabolites in both of the species but their concentrations were variable. Flavanoids and saponins were found in remarkable amount in both of the samples. The volatile compounds of both plants were extracted by Simultaneous Steam Distillation and Extraction (SDE) apparatus and analyzed by Gas Chromatography/Mass Spectrometery (GC-MS). Total 53 volatile compounds were tentatively identified from roots of Dipsacus mitis D. Don and 78 were identified from flowers of Woodfordia fruticosa (L.) Kurz. Alcohols were dominant in both the plants accounting 45% and 32% respectively. Numerous bioactive volatile compounds were detected in both herbs. The effects of increasing concentrations of the methanolic extracts on the amplitude and frequency of spontaneously contracting uterine tissues were tested. The effect of extracts on the smooth muscle strips from rat uterus showes the dramatic muscular relaxation on spontaneous contractility and was determided most effective at a concentration 6500 µg/ml of methanolic extract of Dipsacus mitis D.DOI: http://dx.doi.org/10.3126/jnpa.v26i1.6627JNPA. XXVI(1) 2012 1-11

scholarly journals Mutagenic Activity ofIndigofera truxillensisandI. suffruticosaAerial Parts

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Tamara Regina Calvo ◽  
Cássia Regina Primila Cardoso ◽  
Adriana Candido da Silva Moura ◽  
Lourdes Campaner dos Santos ◽  
Ilce Mara Syllos Colus ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Methanolic Extract ◽  
Mutagenic Activity ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Alkaloid Fraction ◽  
Indigo Dye

Indigofera truxillensisandI. suffruticosa, are used as a source of indigo dye and to treat several diseases. The mutagenic activity of the methanolic extracts from aerial parts, glycerolipid, flavonoid and alkaloid fractions of the extract were evaluated by means ofSalmonella/microsome assays using TA100, TA98, TA102 and TA97a strains. The methanolic extract ofI. truxillensisshowed mutagenic activity in the TA98 strain without S9 while glycerolipid fraction was devoid of activity. The flavonoid and alkaloid fractions of both plants showed mutagenicity. Chemical analysis of flavonoid fractions ofI. truxillensisandI. suffruticosaresulted in the identification of kaempferol, quercetin and their derivatives. The alkaloid fraction of both the species contained indigo and indirubin and indigo was found mainly responsible for the mutagenic activity.

scholarly journals Hair Washing Formulations from Aloe elegans Todaro Gel: The Potential for Making Hair Shampoo

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Desta Berhe Sbhatu ◽  
Goitom Gebreyohannes Berhe ◽  
Abadi Gebreyesus Hndeya ◽  
Asmael Abdu ◽  
Afework Mulugeta ◽  
...  
Keyword(s):  
Foam Stability ◽  
Chemical Constituents ◽  
Coconut Oil ◽  
Capric Acid ◽  
Lemon Juice ◽  
Solid Content ◽  
Phytochemical Screening ◽  
Ms Analysis ◽  
Physicochemical Methods ◽  
Gas Chromatography Mass Spectroscopy

This study aimed to describe the gross phytochemical constituents of Aloe elegans Todaro gel and evaluate the characteristics and quality of lab-made hair washing formulations prepared from the gel to show its potential in formulating hair washing shampoos. A. elegans gel mass was prepared from mature, healthy leaves collected from natural stand. Samples of 100% methanol extract of the gel were subjected to standard phytochemical screening and gas chromatography-mass spectroscopy (GC-MS) analysis. Five hair washing formulations (Fs) were, likewise, prepared by mixing 4.0–10.0 mL of gel with one (0.05 mL) to two (0.10 mL) drops of six synthetic and natural ingredients, namely, coconut oil, jojoba oil, olive oil, pure glycerin oil, lemon juice, and vitamin E. The gel to the total ingredient ratios (v/v) of the five formulations were 93 : 7 (F1), 94.5 : 5.5 (F2), 96.4 : 3.6 (F3), and 96.6 : 3.4 (F4 and F5). The formulations were evaluated using sensory inspection and common physicochemical methods. The phytochemical screening and GC-MS analysis revealed that A. elegans gel is the source of important chemical constituents used in the formulation of shampoos and similar products including saponins, capric acid, lauric acid, myristic acid, palmitic acid, linoleic acid, stearic acid, and phytol. Lab-made A. elegans hair washing formulations, especially those with 96.4–96.6% gel, were found to have similar characteristics and qualities with a common marketed shampoo. All the formulations were turbid with characteristic odor as the marketed shampoo. The pH values of the hair washing formulations (6.4–4.6) were comparable to those of the marketed shampoo (6.7). Formulations with higher proportion of gel had better foam stability, higher solid content (26–29%), higher surface tension (33–38 dynes/cm), shorter wetting time (150–160 sec), equivalent viscosities (26.45–26.73 poise), and conditioning performance than the marketed shampoo. These findings demonstrate that A. elegans gel mass can be used in the formulation of good-quality hair washing shampoos. We recommend future studies that aim to develop the phytochemical profile of the plant and a refined protocol of hair washing shampoo formulation.

scholarly journals Pharmacological evaluation of methanolic extract of Cyperus scariosus

2016 ◽  
Vol 11 (2) ◽  
pp. 353 ◽  
Author(s):  
Wasi Ullah Khan ◽  
Rahmat Ali Khan ◽  
Mushtaq Ahmed ◽  
Latif Ullah Khan ◽  
Muhammad Waqas Khan
Keyword(s):  
Death Rate ◽  
Methanolic Extract ◽  
Biological Activities ◽  
Standard Procedure ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Methanolic Extracts ◽  
Biological Tests ◽  
Β Carotene

<p class="Abstract">The present study is aimed to investigate the phytochemical screening and biological activities of methanolic extract of <em>Cyperus scariosus</em> roots. Dried plant was grounded and extracted with methanol to prepare methanol crud extract. In vitro biological tests were conducted using this methanolic extracts according to the standard procedure. 100% death rate of brine shrimp was perceived at 3 mg/mL of plant extract after 72 hours. The extract showed action against <em>Aspergillus flavius</em> i.e. 90% followed by A. niger (91%) while the highest activity was shown against <em>A. fumegatrus</em> (94%). Important scavenging results were detected during scavenging of free radicals viz; 92.2% against DPPH, 82.2% to ABTS, 75.8% to hydrogen peroxide, 88.1% to β-carotene, 86.1% to hydroxyl radical and 89.4% against phosphomolybdate at 3 mg/mL were obtained. The results obtained in this study point out that extract showed significant biological activities which might be due to the presence of bioactive constituents.</p><p> </p>

scholarly journals PHYTOCHEMICAL ANALYSIS & IN-VITRO ANTI OBESITY ACTIVITY OF DIFFERENT FRACTIONS OF METHANOLIC EXTRACT OF FAGONIA CRETICA L.

2020 ◽  
pp. 282-286
Author(s):  
Divyang Patel ◽  
Vimal Kumar
Keyword(s):  
Inhibitory Action ◽  
Methanolic Extract ◽  
Age Groups ◽  
Phytochemical Analysis ◽  
Phytochemical Study ◽  
Mortality And Morbidity ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Phytochemical Studies

Obesity is one of the most prevalent health concerns among all age groups & populations worldwide, resulting into a significant increase in mortality and morbidity related to metabolic disorders. Targeting one or more enzymes involved in lipid metabolism can be selective for evaluation of anti obesity action of drug. The present study was aimed to evaluate in vitro anti obesity action by inhibiting pancreatic lipase & ᾳ amylase enzyme by various fractions of methanolic extract of aerial parts Fagonia cretica L. along with their phytochemical analysis. The n- hexane (HFFC), Chloroform (CFFC), Ethyl acetate (EAFFC), n-butanol (BFFC) & aqueous fractions (AQFFC) were prepared from methanolic extracts of F. cretica L & were analyzed for qualitative as well as quantitative phytochemical study using reported methods. The qualitative phytochemical studies of prepared extract & fractions showed presence of flavonoids, saponins, phenolics, alkaloids & carbohydrates. All the fractions were then examined for their in-vitro lipase inhibitory & ᾳ amylase inhibitory activities at a concentration level of 50, 100, 150 & 200µg/ml and their percentage inhibitory effects were reported. Among the analyzed samples, BFFC showed highest lipase inhibitory action i.e. 83.02 ± 2.47% as compared to other fractions. EAFFC showed significantly higher ᾳ amylase inhibitory action i.e. 80.22 ± 1.18% as compared to other fractions.

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