Application of Experiential Design for Framing Gastroretentive Microsponges of Glipizide: Screening of Critical Variables by Plackett-Burman Design and Optimization by Box-Behnken Design

Angkak (red mold rice, red yeast rice, Chinese red rice), a traditional Chinese functional food is produced by solid-state fermentation of cooked non-glutinous rice with Monascus species. The secondary metabolite of Monascus species, monacolin K (lovastatin), has been proved to lower blood lipid levels. In this study, the best Monascus purpureus strain was selected from MTCC 369, 410, 1090 based on lovastatin concentration. Four medium parameters (NH4Cl, MgSO4, NaCl, CaCl2) screened by Plackett-Burman design from total nine medium variables were optimised by Box-Behnken design of response surface methodology. Maximum lovastatin production of 3.420 mg/g was predicted in the solid medium containing 20 g rice and 40 ml liquid nutrient (NH4Cl 14.32 g/l, MgSO4 0.76 g/l, NaCl 14.65 g/l, and CaCl2 0.54 g/l) by the point prediction tool of Design Expert Ver. 7.1 software at 14th day of fermentation.

Download Full-text

Application of Plackett-Burman Design and Box-Behnken Design to Achieve Process Optimization for Geniposide Submicron Emulsion

Journal of Dispersion Science and Technology ◽

10.1080/01932691.2011.561162 ◽

2012 ◽

Vol 33 (2) ◽

pp. 213-222 ◽

Cited By ~ 3

Author(s):

Peng-Fei Yue ◽

Qin Zheng ◽

Bin Wu ◽

Peng-Yi Hu ◽

Zhen-Feng Wu ◽

...

Keyword(s):

Process Optimization ◽

Box Behnken Design ◽

Burman Design ◽

Submicron Emulsion ◽

Plackett Burman Design

Download Full-text

DESIGN OF CULTURAL CONDITIONS FOR PRODUCTION OF CHONDROITINASE BY ASPERGILLUS NIGER USING DESIGN OF EXPERIMENTS

INDIAN DRUGS ◽

10.53879/id.52.06.10275 ◽

2015 ◽

Vol 52 (06) ◽

pp. 24-32

Author(s):

N Kasinathan ◽

◽

V. M. Subrahmanyam ◽

V. R Josyula

Keyword(s):

Design Of Experiments ◽

Yeast Extract ◽

Spinal Cord Injuries ◽

Box Behnken Design ◽

Medium Components ◽

Cultural Conditions ◽

Burman Design ◽

Level Of Significance ◽

Optimized Conditions ◽

Plackett Burman Design

Chondroitinases are useful in treating cancer and spinal cord injuries. The work reported so far does not focus on production of fungal chondroitinase. The present study focuses on the optimization of cultural conditions using design of experiments for production of chondroitinase by a fungal isolate CHCS-2, obtained from humus containing poultry waste. The cultural conditions were optimized systematically using a one-factor-at-a-time (OFAT) approach, factorial design, Plackett-Burman design (PBD) and Box- Behnken design to identify the composition of the production medium and the level of the variables. The level of significance of the medium components selected by OFAT study viz., yeast extract, chondroitin, and phosphate, was analyzed by factorial studies. The effect of eleven minerals on the enzyme yield was studied by PBD. The interaction among the medium components selected using factorial and PBD viz., yeast extract, cobalt and aluminum was investigated using the Box-Behnken design (BBD). Under the optimized conditions, maximum chondroitinase yield was 22.5 U/mL.

Download Full-text

Improved Production of Sublancin 168 Biosynthesized byBacillus subtilis168 Using Chemometric Methodology and Statistical Experimental Designs

BioMed Research International ◽

10.1155/2015/687915 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Shengyue Ji ◽

Weili Li ◽

Haiyun Xin ◽

Shan Wang ◽

Binyun Cao

Keyword(s):

Soybean Meal ◽

Incubation Temperature ◽

Optimal Combination ◽

Fermentation Condition ◽

Box Behnken Design ◽

Steepest Ascent ◽

Burman Design ◽

Fermentation Parameters ◽

Optimization Of Fermentation ◽

Plackett Burman Design

Sublancin 168, as a distinct S-linked antimicrobial glycopeptide produced byBacillus subtilis168, is effective in killing specific microorganisms. However, the reported yield of sublancin 168 is at a low level of no more than 60 mg from 1 L fermentation culture ofB. subtilis168 by using the method in the literature. Thus optimization of fermentation condition for efficiently producing sublancin 168 is required. Here, Box-Behnken design was used to determine the optimal combination of three fermentation parameters, namely, corn powder, soybean meal, and temperature that were identified previously by Plackett-Burman design and the steepest ascent experiment. Subsequently, based on the response surface methodology, the quadratic regression model for optimally producing sublancin 168 was developed, and the optimal combination of culture parameters for maximum sublancin 168 production of 129.72 mg/L was determined as corn powder 28.49 g/L, soybean meal 22.99 g/L, and incubation temperature 30.8°C. The results showed that sublancin 168 production obtained experimentally was coincident with predicted value of 125.88 mg/L, and the developed model was proved to be adequate, and the aim of efficiently producing sublancin 168 was achieved.

Download Full-text

Formulation and Statistical Optimization of Culture Medium for Improved Production of Antimicrobial Compound byStreptomycessp. JAJ06

International Journal of Microbiology ◽

10.1155/2013/526260 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 16

Author(s):

Polpass Arul Jose ◽

Kunjukrishnan Kamalakshi Sivakala ◽

Solomon Robinson David Jebakumar

Keyword(s):

Antibiotic Production ◽

Polynomial Equation ◽

Optimization Method ◽

Quadratic Model ◽

Production Medium ◽

Box Behnken Design ◽

Enhanced Production ◽

Burman Design ◽

Plackett Burman Design ◽

Salt Formulation

Streptomycessp. JAJ06 is a seawater-dependent antibiotic producer, previously isolated and characterised from an Indian coastal solar saltern. This paper reports replacement of seawater with a defined salt formulation in production medium and subsequent statistical media optimization to ensure consistent as well as improved antibiotic production byStreptomycessp. JAJ06. This strain was observed to be proficient to produce antibiotic compound with incorporation of chemically defined sodium-chloride-based salt formulation instead of seawater into the production medium. Plackett-Burman design experiment was applied, and three media constituents, starch, KBr, and CaCO3, were recognised to have significant effect on the antibiotic production ofStreptomycesJAJ06 at their individual levels. Subsequently, Response surface methodology with Box-Behnken design was employed to optimize these influencing medium constituents for the improved antibiotic production ofStreptomycessp. JAJ06. A total of 17 experiments were conducted towards the construction of a quadratic model and a second-order polynomial equation. Optimum levels of medium constituents were obtained by analysis of the model and numerical optimization method. When the strain JAJ06 was cultivated in the optimized medium, the antibiotic activity was increased to 173.3 U/mL, 26.8% increase as compared to the original (136.7 U/mL). This study found a useful way to cultivateStreptomycessp. JAJ06 for enhanced production of antibiotic compound.

Download Full-text

Optimization of Cultural Conditions for Antioxidant Exopolysaccharides fromXerocomus badiusGrown in Shrimp Byproduct

BioMed Research International ◽

10.1155/2016/2043787 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 2

Author(s):

Xiujun Gao ◽

Peisheng Yan ◽

Xin Liu ◽

Jianbing Wang ◽

Jiajia Yu

Keyword(s):

Antioxidant Activity ◽

Regression Models ◽

Antioxidant Activities ◽

High Yield ◽

Box Behnken Design ◽

Steepest Ascent ◽

Cultural Conditions ◽

Total Antioxidant ◽

Burman Design ◽

Plackett Burman Design

To optimize the production conditions for exopolysaccharides with higher antioxidant activities fromXerocomus badiuscultured in shrimp byproduct medium, Plackett-Burman design, path of steepest ascent, and response surface methodology were explored. Based on the results of Plackett-Burman design and path of steepest ascent, a Box-Behnken design was applied to optimization and the regression models. The optimal cultural condition for high yield and antioxidant activity of the exopolysaccharides was determined to be 10.347% of solid-to-liquid ratio, a 4.322% content of bran powder, and a 1.217% concentration of glacial acetic acid. Culturing with the optimal cultural conditions resulted in an exopolysaccharides yield of4.588±0.346 g/L and a total antioxidant activity of2.956±0.105 U/mg. These values are consistent with the values predicted by the corresponding regression models (RSD < 5%).

Download Full-text

Optimization of Selenium Yeild by Saccharomyces sp. III Using Statistical Designs

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.781-784.1278 ◽

2013 ◽

Vol 781-784 ◽

pp. 1278-1283

Author(s):

Na Xin Sun ◽

Gui Xiang Zhang ◽

Yue Hui Liu ◽

Yuan Xiu Wang

Keyword(s):

Sodium Selenite ◽

Culture Medium ◽

Screening Method ◽

Liquid Volume ◽

Statistical Experimental Design ◽

Box Behnken Design ◽

Burman Design ◽

Statistical Designs ◽

Plackett Burman Design ◽

Dry Cell

The objective of the study was to optimize the conditions in a culture medium for the selenium yeild enriched by Saccharomyces sp. III using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the significant factors in the fermentation for the selenium yeild, and subsequent use of the response surface methodology was further optimized for the selenium yeild by Box-Behnken design. The important factors in the culture medium, identified by the initial screening method of Placket-Burman, were sodium selenite, glucose and the liquid volume. The optimal amounts for maximum selenium yeild were: sodium selenite 15.8 mg/L; glucose 40.2 g/L; the liquid volume 120 mL in 250 mL flask. Using this statistical experimental design, the selenium yeild under optimal condition reached about 1679.32 μg selenium /g dry cell.

Download Full-text

Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

10.21203/rs.2.16618/v2 ◽

2020 ◽

Author(s):

Noura El-Ahmady El-Naggar ◽

Nancy M. El-Shweihy

Keyword(s):

Yeast Extract ◽

Cholesterol Oxidase ◽

Agitation Speed ◽

Isolation And Identification ◽

Box Behnken Design ◽

Positive Effects ◽

Wide Range ◽

Burman Design ◽

Promising Source ◽

Plackett Burman Design

Abstract Background: Cholesterol oxidase biosensors have been used to determine the level of cholesterol in different serum and food samples. Due to a wide range of industrial and clinical applications of microbial cholesterol oxidase, isolation and identification of a new microbial source (s) of cholesterol oxidase are very important. Results: The local isolate Streptomyces sp. strain NEAE-94 is a promising source of cholesterol oxidase. It was identified based on cultural, morphological and physiological characteristics; in addition to the 16S rRNA sequence. The sequencing product had been deposited in the GenBank database under the accession number KC354803. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 in shake flasks was optimized using surface response methodology. The different process parameters were first screened using a Plackett-Burman design and the parameters with significant effects on the production of cholesterol oxidase were identified. Out of the fifteen factors screened, agitation speed, cholesterol and yeast extract concentrations had the most significant positive effects on the production of cholesterol oxidase. The optimal levels of these variables and the effects of their mutual interactions on cholesterol oxidase production were determined using Box-Behnken design. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 was 11.03, 27.31 U/mL after Plackett-Burman Design and Box-Behnken design; respectively, with a fold of increase of 6.06 times compared to the production before applying the Plackett-Burman design (4.51 U/mL). Conclusions: Maximum cholesterol oxidase activity is obtained at the following fermentation conditions: g/L (cholesterol 4, yeast extract 5, NaCl 0.5, K 2 HPO 4 1, FeSO 4 .7H 2 O 0.01, MgSO 4 .7H 2 O 0.5), pH 7, inoculum size 4 % (v/v), temperature 37°C, agitation speed 150 rpm, medium volume 50 mL and incubation time 5 days.

Download Full-text