DESIGN OF CULTURAL CONDITIONS FOR PRODUCTION OF CHONDROITINASE BY ASPERGILLUS NIGER USING DESIGN OF EXPERIMENTS

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (06) ◽  
pp. 24-32
Author(s):  
N Kasinathan ◽  
◽  
V. M. Subrahmanyam ◽  
V. R Josyula
Keyword(s):  
Design Of Experiments ◽  
Yeast Extract ◽  
Spinal Cord Injuries ◽  
Box Behnken Design ◽  
Medium Components ◽  
Cultural Conditions ◽  
Burman Design ◽  
Level Of Significance ◽  
Optimized Conditions ◽  
Plackett Burman Design

Chondroitinases are useful in treating cancer and spinal cord injuries. The work reported so far does not focus on production of fungal chondroitinase. The present study focuses on the optimization of cultural conditions using design of experiments for production of chondroitinase by a fungal isolate CHCS-2, obtained from humus containing poultry waste. The cultural conditions were optimized systematically using a one-factor-at-a-time (OFAT) approach, factorial design, Plackett-Burman design (PBD) and Box- Behnken design to identify the composition of the production medium and the level of the variables. The level of significance of the medium components selected by OFAT study viz., yeast extract, chondroitin, and phosphate, was analyzed by factorial studies. The effect of eleven minerals on the enzyme yield was studied by PBD. The interaction among the medium components selected using factorial and PBD viz., yeast extract, cobalt and aluminum was investigated using the Box-Behnken design (BBD). Under the optimized conditions, maximum chondroitinase yield was 22.5 U/mL.

scholarly journals Optimization of Cultural Conditions for Antioxidant Exopolysaccharides fromXerocomus badiusGrown in Shrimp Byproduct

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Xiujun Gao ◽  
Peisheng Yan ◽  
Xin Liu ◽  
Jianbing Wang ◽  
Jiajia Yu
Keyword(s):  
Antioxidant Activity ◽  
Regression Models ◽  
Antioxidant Activities ◽  
High Yield ◽  
Box Behnken Design ◽  
Steepest Ascent ◽  
Cultural Conditions ◽  
Total Antioxidant ◽  
Burman Design ◽  
Plackett Burman Design

To optimize the production conditions for exopolysaccharides with higher antioxidant activities fromXerocomus badiuscultured in shrimp byproduct medium, Plackett-Burman design, path of steepest ascent, and response surface methodology were explored. Based on the results of Plackett-Burman design and path of steepest ascent, a Box-Behnken design was applied to optimization and the regression models. The optimal cultural condition for high yield and antioxidant activity of the exopolysaccharides was determined to be 10.347% of solid-to-liquid ratio, a 4.322% content of bran powder, and a 1.217% concentration of glacial acetic acid. Culturing with the optimal cultural conditions resulted in an exopolysaccharides yield of4.588±0.346 g/L and a total antioxidant activity of2.956±0.105 U/mg. These values are consistent with the values predicted by the corresponding regression models (RSD < 5%).

scholarly journals Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design

2020 ◽  
Vol 8 (6) ◽  
pp. 1250-1255
Author(s):  
Fikriye Alev Akçay ◽  
Ayşe Avcı
Keyword(s):  
Yeast Extract ◽  
Protease Activity ◽  
Inoculum Size ◽  
Protease Production ◽  
Bacillus Sp ◽  
Medium Components ◽  
Dipotassium Phosphate ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Selection Of

In this study, effects of medium components and inoculum size on the protease production by Bacillus sp. EBTA6 that was isolated from a home-made Tarhana sample were investigated. The cell-free supernatant of bacterium cultured on a shaking incubator for 24 h was used to test protease activity as the response. With a total number of 11 factors, 12 different experiments were run and the highest experimental protease activity was measured as 2280.4 U/mL. Results were analyzed statistically by ANOVA and the most efficient factors were detected as yeast extract, dipotassium phosphate, casein, and peptone with a contribution of 93.78, 2.19, 1.96, 1.31%, respectively. For validation of the selected factors, a further experiment was performed by using of yeast extract (9.98 g/L), dipotassium phosphate (1.27 g/L), casein (8.69 g/L), and peptone (9.88 g/L) obtained from the design equation. The experimental response was found as 2411.4 U/mL which was only 5.5% higher than the predicted response showing that the model was applicable.

scholarly journals Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

2020 ◽  
Author(s):  
Noura El-Ahmady El-Naggar ◽  
Nancy M. El-Shweihy
Keyword(s):  
Yeast Extract ◽  
Cholesterol Oxidase ◽  
Agitation Speed ◽  
Isolation And Identification ◽  
Box Behnken Design ◽  
Positive Effects ◽  
Wide Range ◽  
Burman Design ◽  
Promising Source ◽  
Plackett Burman Design

Abstract Background: Cholesterol oxidase biosensors have been used to determine the level of cholesterol in different serum and food samples. Due to a wide range of industrial and clinical applications of microbial cholesterol oxidase, isolation and identification of a new microbial source (s) of cholesterol oxidase are very important. Results: The local isolate Streptomyces sp. strain NEAE-94 is a promising source of cholesterol oxidase. It was identified based on cultural, morphological and physiological characteristics; in addition to the 16S rRNA sequence. The sequencing product had been deposited in the GenBank database under the accession number KC354803. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 in shake flasks was optimized using surface response methodology. The different process parameters were first screened using a Plackett-Burman design and the parameters with significant effects on the production of cholesterol oxidase were identified. Out of the fifteen factors screened, agitation speed, cholesterol and yeast extract concentrations had the most significant positive effects on the production of cholesterol oxidase. The optimal levels of these variables and the effects of their mutual interactions on cholesterol oxidase production were determined using Box-Behnken design. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 was 11.03, 27.31 U/mL after Plackett-Burman Design and Box-Behnken design; respectively, with a fold of increase of 6.06 times compared to the production before applying the Plackett-Burman design (4.51 U/mL). Conclusions: Maximum cholesterol oxidase activity is obtained at the following fermentation conditions: g/L (cholesterol 4, yeast extract 5, NaCl 0.5, K 2 HPO 4 1, FeSO 4 .7H 2 O 0.01, MgSO 4 .7H 2 O 0.5), pH 7, inoculum size 4 % (v/v), temperature 37°C, agitation speed 150 rpm, medium volume 50 mL and incubation time 5 days.

Enhancement of Hydrogen Production from Sugarcane Bagasse by Klebsiella pneumoniae subsp. pneumonia DSM 30104(T) Using Statistical Methods

2017 ◽  
Vol 757 ◽  
pp. 146-150
Author(s):  
Piyawadee Saraphirom ◽  
Mullika Teerakul ◽  
Manit Anyabho
Keyword(s):  
Potassium Chloride ◽  
Optimal Condition ◽  
Calcium Chloride ◽  
Sugarcane Bagasse ◽  
Yeast Extract ◽  
Total Sugar ◽  
Sugarcane Bagasse Hydrolysate ◽  
Box Behnken Design ◽  
Burman Design ◽  
Plackett Burman Design

This study employed statistically based experimental designs to optimize environmental factors for H2 production from sugarcane bagasse hydrolysate by K. pneumoniae subsp. Pneumonia DSM 30104(T) isolated from wastewater sludge using statistical method. The 12 runs of Plackett-Burman design were used to classify important factors influencing the H2 production from sugarcane bagasse hydrolysate by K. pneumonia subsp. pneumonia DSM 30104(T). Mutual interaction between the significant factor and their optimal values that brought the maximum H2 production (mL H2/L) were further investigated using Box-Behnken design of response surface method. Experimental results indicated that yeast extract, ammonium chloride, potassium chloride, calcium chloride, iron (II) sulfate and total sugar of sugarcane bagasse had an interdependent effect on the maximum H2 production while only interaction effect between yeast extract and calcium chloride had statistically significant (P≤0.05) influences on the maximum H2 production. Optimal conditions for the predicted maximal H2 production were 7.50 g/L yeast extract, 0.50 g/L ammonium chloride, 15.0 g/L potassium chloride, 0.75 g/L calcium chloride, and 10.0 g/L total sugar. At the optimal condition, the maximum H2 production of 277 mL H2/L was estimated from Box-Behnken design that more than 9 times compared to Plackett-Burman design. The highest ratio of butyric acid to acetic acid (B/A ratio) of 1.47 was indicated the high performance of H2 fermentation of sugarcane bagasse hydrolysate by K. pneumoniae subsp. Pneumonia DSM 30104(T) under the optimal condition obtained.

scholarly journals Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

2020 ◽  
Author(s):  
Noura El-Ahmady El-Naggar ◽  
Nancy M. El-Shweihy
Keyword(s):  
Yeast Extract ◽  
Cholesterol Oxidase ◽  
Agitation Speed ◽  
Isolation And Identification ◽  
Box Behnken Design ◽  
Positive Effects ◽  
Wide Range ◽  
Burman Design ◽  
Promising Source ◽  
Plackett Burman Design

Abstract Background: Cholesterol oxidase biosensors have been used to determine the level of cholesterol in different serum and food samples. Due to a wide range of industrial and clinical applications of microbial cholesterol oxidase, isolation and identification of a new microbial source (s) of cholesterol oxidase are very important. Results: The local isolate Streptomyces sp. strain NEAE-94 is a promising source of cholesterol oxidase. It was identified based on cultural, morphological and physiological characteristics; in addition to the 16S rRNA sequence. The sequencing product had been deposited in the GenBank database under the accession number KC354803. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 in shake flasks was optimized using surface response methodology. The different process parameters were first screened using a Plackett-Burman design and the parameters with significant effects on the production of cholesterol oxidase were identified. Out of the fifteen factors screened, agitation speed, cholesterol and yeast extract concentrations had the most significant positive effects on the production of cholesterol oxidase. The optimal levels of these variables and the effects of their mutual interactions on cholesterol oxidase production were determined using Box-Behnken design. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 was 11.03, 27.31 U/mL after Plackett-Burman Design and Box-Behnken design; respectively, with a fold of increase of 6.06 times compared to the production before applying the Plackett-Burman design (4.51 U/mL). Conclusions: Maximum cholesterol oxidase activity is obtained at the following fermentation conditions: g/L (cholesterol 4, yeast extract 5, NaCl 0.5, K 2 HPO 4 1, FeSO 4 .7H 2 O 0.01, MgSO 4 .7H 2 O 0.5), pH 7, inoculum size 4 % (v/v), temperature 37°C, agitation speed 150 rpm, medium volume 50 mL and incubation time 5 days.

Plackett-Burman Design for Media Nutrients of Biocontrol Lysinibacillus xylanilyticus BPM1 against Aflatoxin

2014 ◽  
Vol 522-524 ◽  
pp. 295-298
Author(s):  
Kai Wang ◽  
Pei Sheng Yan ◽  
Li Xin Cao
Keyword(s):  
Biological Control ◽  
Yeast Extract ◽  
Antagonistic Activity ◽  
Medium Composition ◽  
Peanut Hulls ◽  
Two Factors ◽  
Burman Design ◽  
Soya Peptone ◽  
Plackett Burman Design ◽  
Biocontrol Bacterium

Aflatoxins (AFs) are a series of highly toxic and carcinogenic secondary metabolites. In order to eliminate AFs contamination, biological control is one of the more promising techniques. In this study, we describe the optimization of media nutrients for the selected biocontrol bacterium, Lysinibacillus xylanilyticus strain BPM1. The strain was isolated from the peanut hulls in Shandong Province, China and exhibited antagonistic activity against aflatoxins. Maltose and sucrose were identified as best carbon source, while soya peptone and yeast extract as nitrogen source led to the highest OD600 observations. Medium composition was optimized using Plackett-Burman design, which was applied to find the key ingredients. The results revealed that the most significant two factors which were more effective in the fermentation of L. xylanilyticus BPM1 were soya peptone and yeast extract.

scholarly journals Engineering rice based medium for production of lovastatin with Monascus species

2009 ◽  
Vol 27 (No. 5) ◽  
pp. 352-360 ◽  
Author(s):  
B.P. Panda ◽  
S. Javed ◽  
M. Ali
Keyword(s):  
Blood Lipid ◽  
Red Yeast Rice ◽  
Red Rice ◽  
Box Behnken Design ◽  
Glutinous Rice ◽  
Lower Blood ◽  
Red Mold Rice ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Blood Lipid Levels

Angkak (red mold rice, red yeast rice, Chinese red rice), a traditional Chinese functional food is produced by solid-state fermentation of cooked non-glutinous rice with <i>Monascus</i> species. The secondary metabolite of <i>Monascus</i> species, monacolin K (lovastatin), has been proved to lower blood lipid levels. In this study, the best <i>Monascus purpureus</i> strain was selected from MTCC 369, 410, 1090 based on lovastatin concentration. Four medium parameters (NH<sub>4</sub>Cl, MgSO<sub>4</sub>, NaCl, CaCl<sub>2</sub>) screened by Plackett-Burman design from total nine medium variables were optimised by Box-Behnken design of response surface methodology. Maximum lovastatin production of 3.420 mg/g was predicted in the solid medium containing 20 g rice and 40 ml liquid nutrient (NH<sub>4</sub>Cl 14.32 g/l, MgSO<sub>4</sub> 0.76 g/l, NaCl 14.65 g/l, and CaCl<sub>2</sub> 0.54 g/l) by the point prediction tool of Design Expert Ver. 7.1 software at 14<sup>th</sup> day of fermentation.

scholarly journals Enzymatic Extraction, Purification, and Characterization of Polysaccharides from Penthorum chinense Pursh: Natural Antioxidant and Anti-Inflammatory

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Li-mei Lin ◽  
Ling-jia Zhao ◽  
Jing Deng ◽  
Su-hui Xiong ◽  
Jie Tang ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Steepest Ascent ◽  
Penthorum Chinense Pursh ◽  
Ft Ir ◽  
Experimental Yield ◽  
Burman Design ◽  
Anti Inflammatory ◽  
Penthorum Chinense ◽  
Optimized Conditions ◽  
Plackett Burman Design

Penthorum chinense Pursh (PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-α, and IL-1β release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.

scholarly journals Medium Optimization for Spore Production of a Straw-Cellulose Degrading Actinomyces Strain under Solid-State Fermentation Using Response Surface Method

2020 ◽  
Vol 12 (21) ◽  
pp. 8893
Author(s):  
Huanran Liu ◽  
Dan Zhang ◽  
Xia Zhang ◽  
Chuanzhi Zhou ◽  
Pei Zhou ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Spore Production ◽  
Steepest Ascent ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

The strains capable of degrading cellulose have attracted much interest because of their applications in straw resource utilization in solid-state fermentation (SSF). However, achieving high spore production in SSF is rarely reported. The production of spores from Streptomyces griseorubens JSD-1 was investigated in shaker-flask cultivation in this study. The optimal carbon, organic nitrogen and inorganic nitrogen sources were sucrose, yeast extract and urea, respectively. Plackett–Burman design (PBD) was adopted to determine the key medium components, and the concentration levels of three components (urea, NaCl, MgSO4·7H2O) were optimized with the steepest ascent path and central composite design (CCD), achieving 1.72 × 109 CFU/g of spore production. Under the optimal conditions (urea 2.718% w/v, NaCl 0.0697% w/v, MgSO4·7H2O 0.06956% w/v), the practical value of spore production was 1.69 × 109 CFU/g. The determination coefficient (R2) was 0.9498, which ensures an adequate credibility of the model.

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