scholarly journals Inheritance and Allelic Relationships of Alectra vogelii Benth. Resistance Genes in Cowpea Genotypes B301 and KVx414-22-2

2021 ◽  
Vol 13 (8) ◽  
pp. 10
Author(s):  
Zakaria Dieni ◽  
Jean-Baptiste De La Salle Tignegre ◽  
Benoit T. Joseph Batieno ◽  
Felicien W. M. Serge Zida ◽  
Abdou Kader Congo
Keyword(s):  
Burkina Faso ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Goodness Of Fit ◽  
Dominant Gene ◽  
Research Station ◽  
Single Dominant Gene ◽  
Goodness Of Fit Test ◽  
Allelic Relationship ◽  
Resistant Varieties

Alectra vogelii Benth. is the second most important parasitic weed in cowpea [Vigna unguiculata (L.) Walp.] production in Burkina Faso. Several resistant varieties to this weed have been identified in the country among which are B301 and KVx414-22-2. The inheritance and allelic relationships of the resistance genes in the two varieties have not been studied with A. vogelii strains in Burkina Faso. The objective of this study was to determine the inheritance and allelic relationship of the resistance genes in B301 and KVx414-22-2. To determine the inheritance of the genes for resistance, the resistant varieties (B301 and KVx414-22-2) were each crossed to a susceptible variety IT82D-849 to generate F1 and F2 populations. For the allelic relationship study the two resistant genotypes were crossed among themselves to generate F1 and F2 offspring. The parents and their F1 and F2 progenies were screened in artificially infested pots with Alectra seed in a screen house at Kamboinsé Research Station in Burkina Faso. Resistance/susceptibility of genotypes was assessed by recording the number of emerged Alectra shoots. The data were subjected to the Chi-Square goodness-of-fit test for one, two and three genes segregation ratios. The results revealed that two independent dominant genes confer resistance in the variety B301 and a single dominant gene confers resistance in variety KVx414-22-2. The single dominant gene in KVx414-22-2 is non-allelic to the two genes in B301. The two resistance genes in variety B301 have already been named Rav1 and Rav2 whilst Rav3 is the name of the resistance gene in variety IT81D-994. Therefore, we propose the symbol Rav4 as the name for the resistance gene in variety KVx414-22-2.

scholarly journals A Novel Pear Scab (Venturia nashicola) Resistance Gene, Rvn3, from Interspecific Hybrid Pear (Pyrus pyrifolia × P. communis)

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2632
Author(s):  
Sewon Oh ◽  
Hyeondae Han ◽  
Daeil Kim
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Interspecific Hybrid ◽  
Dominant Gene ◽  
Apple Scab ◽  
Scab Resistance ◽  
Hybrid Population ◽  
Pyrus Pyrifolia ◽  
Pear Scab ◽  
Venturia Nashicola

Asian pear scab is a fungal disease caused by Venturia nashicola. The identification of genes conferring scab resistance could facilitate the breeding of disease-resistant cultivars. Therefore, the present study aimed to identify a scab-resistance gene using an interspecific hybrid population ((Pyrus pyrifolia × P. communis) × P. pyrifolia). Artificial inoculation of V. nashicola was carried out for two years. The segregation ratio (1:1) of resistant to susceptible individuals indicated that resistance to V. nashicola was inherited from P. communis and controlled by a single dominant gene. Based on two years phenotypic data with the Kruskal–Wallis test and interval mapping, 12 common markers were significantly associated with scab resistance. A novel scab resistance gene, Rvn3, was mapped in linkage group 6 of the interspecific hybrid pear, and co-linearity between Rvn3 and one of the apple scab resistance genes, Rvi14, was confirmed. Notably, an insertion in pseudo-chromosome 6 of the interspecific hybrid cultivar showed homology with apple scab resistance genes. Hence, the newly discovered Rvn3 was considered an ortholog of the apple scab resistance gene. Since the mapping population used in the present study is a pseudo-BC1 population, pyramiding of multiple resistance genes to pseudo-BC1 could facilitate the breeding of pear cultivars with durable resistance.

scholarly journals Genetics of stem rust resistance in four popular durum wheat cultivars

2020 ◽  
Vol 80 (02) ◽  
Author(s):  
A. Divya ◽  
T. L. Prakasha ◽  
S. Chand ◽  
A. N. Mishra ◽  
V. G. Dubey ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Resistance Genes ◽  
Stem Rust ◽  
Rust Resistance ◽  
Dominant Gene ◽  
Central India ◽  
Stem Rust Resistance ◽  
Single Dominant Gene ◽  
Wheat Cultivars ◽  
Segregation Data

A study was conducted to understand the mode of inheritance and extent of diversity of stem rust resistance in four popular durum wheat cultivars of central India viz., HI 8498 (Malav Shakti), HI 8663 (Poshan), HI 8713 (Pusa Mangal) and HI 8737 (Pusa Anmol) using Puccinia graminis tritici (Pgt) pathotypes 15-1 (123G15) and 40-3 (127G29). These cultivars were crossed with susceptible parents i.e., Motia and Malvi Local and were also crossed among themselves in half diallel fashion. The F2 and F3 segregation data revealed that a single dominant gene each controlled resistance to the pathotype 40-3 in HI 8713 and HI 8663, while two dominant genes each governed resistance to this pathotype in HI 8737 and HI 8498. A single dominant gene each conditioned resistance to the pathotype 15-1 in all the four cultivars. The F2 segregation data of the intercrosses among the resistant parents showed that three different resistance genes controlled resistance among four cultivars against each Pgt pathotype 40-3 and 15-1. These genes seem to be different from the most commonly postulated stem rust resistance genes in Indian durum wheat germplasm viz., Sr11, Sr12, Sr7b and Sr9e which are ineffective/less effective against the test pathotypes. Hence, the genes identified in the present study can be utilized in broadening the genetic base of stem rust resistance in Indian durum wheat.

scholarly journals Inheritance of anthracnose resistance in common bean genotypes P.I. 207262 and AB 136

1997 ◽  
Vol 20 (1) ◽  
pp. 59-62 ◽  
Author(s):  
M.C. Gonçalves-Vidigal ◽  
Antônio A. Cardoso ◽  
Clibas Vieira ◽  
Luiz S. Saraiva
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Resistance Genes ◽  
Dominant Gene ◽  
Colletotrichum Lindemuthianum ◽  
Single Dominant Gene ◽  
Anthracnose Resistance ◽  
Dominant Genes

Bean (Phaseolus vulgaris) lines P.I. 207262 and AB 136, both resistant to delta and kappa races of Colletotrichum lindemuthianum, were crossed with Michelite, Dark Red Kidney, and Perry Marrow, susceptible to both races, and with Cornell 49-242, resistant to delta and susceptible to kappa. F1 and F2 reactions demonstrated that P.I. 207262 carries duplicate dominant genes for resistance to the delta race; AB 136 carries a dominant gene. These resistance genes are independent of the Are gene from Cornell 49-242. With respect to the kappa race, F1 and F2 data showed that the resistance controlled by P.I. 207262 and by AB 136 depends on a single dominant gene. Complementary factors were involved with AB 136 resistance to the delta race and with P.I. 207262 resistance to kappa.

scholarly journals Inheritance and Allelic Relationship Among Downy Mildew Resistance Genes in Pearl Millet

Plant Disease ◽  
2018 ◽  
Vol 102 (6) ◽  
pp. 1136-1140 ◽  
Author(s):  
Chandramani Raj ◽  
Rajan Sharma ◽  
B. Pushpavathi ◽  
S. K. Gupta ◽  
K. Radhika
Keyword(s):  
Downy Mildew ◽  
Pearl Millet ◽  
Dominant Gene ◽  
Single Dominant Gene ◽  
Susceptible Parent ◽  
Sclerospora Graminicola ◽  
Resistant Parent ◽  
Disease Reaction ◽  
Allelic Relationship ◽  
Dominant Genes

Pearl millet downy mildew (DM), caused by Sclerospora graminicola, is of serious economic concern to pearl millet farmers in the major crop-growing areas of the world. To study the inheritance and allelic relationship among genes governing resistance to this disease, three DM-resistant pearl millet lines (834B, IP 18294-P1, and IP 18298-P1) and one susceptible line (81B) were selected on the basis of disease reaction under greenhouse conditions against two isolates of S. graminicola (Sg 526-1 and Sg 542-1). Three resistant parents were crossed with the susceptible parent to generate F1, F2, and backcross BC1P1 (susceptible parent × F1) and BC1P2 (resistant parent × F1) generations for inheritance study. To carry out a test for allelism, the three resistant parents were crossed with each other to generate F1 and F2 generations. The different generations of these crosses were screened for disease reaction against two isolates (Sg 526-1 and Sg 542-1) by artificial inoculation under greenhouse conditions. The segregation pattern of resistance in the F2 and corresponding backcross generations revealed that resistance to DM is controlled by a single dominant gene in 834B and IP 18294-P1 and by two dominant genes in IP 18298-P1. A test for allelism inferred that a single dominant gene for resistance in 834B is nonallelic to that which governs resistance in IP 18294-1, whereas one of the two dominant genes for DM resistance in IP 18298-P1 against the test isolates is allelic to the gene for DM resistance in 834B and a second gene is allelic to the resistance gene present in IP 18294-P1.

scholarly journals Mapping of resistance genes to races 1, 3 and 5 of Podosphaera xanthii in melon PI 414723

2013 ◽  
Vol 13 (4) ◽  
pp. 349-355 ◽  
Author(s):  
Ana Carolina Fazza ◽  
Leandro José Dallagnol ◽  
Ana Cristina Fazza ◽  
Carolina C. Monteiro ◽  
Bruno Marco de Lima ◽  
...  
Keyword(s):  
Linkage Group ◽  
Genetic Map ◽  
Resistance Genes ◽  
Segregation Analysis ◽  
Specific Resistance ◽  
Dominant Gene ◽  
Inheritance Of Resistance ◽  
Podosphaera Xanthii ◽  
Single Dominant Gene ◽  
First Time

The fungus Podosphaera xanthii affects melon crops and presents several races controlled by race-specific resistance genes. The accession PI 414723 is resistant to races 1, 3 and 5 and it is a suitable source of resistance genes. The inheritance of resistance to these races was analyzed on 87 F2 plants from the cross of PI 414723 × Védrantais, and resistance to all three races could be explained by the segregation of a single dominant gene, although a digenic model could also be accepted. A genetic map was assembled with 206 markers, and co-segregation analysis of resistance phenotypes indicated the existence of two linked loci in linkage group II, one conferring resistance to races 1 and 5 (denominated Pm-x1,5), and the second to race 3 (denominated Pm-x3), located 5.1 cM apart. This study reports for the first time the existence of Pm-x3 and the genetic locations of these resistance genes from PI 414723.

Genetic map of the powdery mildew resistance gene in soybean PI 243540

Genome ◽  
2010 ◽  
Vol 53 (5) ◽  
pp. 400-405 ◽  
Author(s):  
Sung-Taeg Kang ◽  
M.A. Rouf Mian
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Dominant Gene ◽  
Common Disease ◽  
Single Dominant Gene ◽  
Powdery Mildew Resistance Gene ◽  
Chromosome 16 ◽  
Mildew Resistance

Powdery mildew (caused by Microsphaera diffusa Cooke & Peck) is a common disease of soybean in many soybean-growing regions of the world and under greenhouse conditions. The previously reported Rmd locus of soybean for resistance to powdery mildew was mapped on soybean molecular linkage group J (chromosome 16). We have discovered a single dominant gene in PI 243540 that provides season-long resistance to powdery mildew. The objective of this study was to map the powdery mildew resistance gene in PI 243540 with PCR-based molecular markers. One hundred eighty-four F2 plants and their F2:3 families from a cross between the powdery mildew susceptible cultivar ‘Wyandot’ and PI 243540 were screened with M. diffusa in greenhouses. Bulked segregant analysis (BSA) with SSR markers was used to identify the tentative genomic location of the gene. The BSA localized the gene to a genomic region in soybean chromosome 16. A linkage map with seven SSR and six SNP markers flanking the gene was constructed. We positioned the gene between SSR marker Sat_224 and SNP marker BARC-021875-04228 at distances of 9.6 and 1.3 cM from the markers, respectively. The map position of the gene was slightly different from previously reported map positions of the only known Rmd locus. We have mapped a single dominant gene, tentatively called Rmd_PI243540, near the previously known Rmd locus on chromosome 16. The molecular markers flanking the gene will be useful for marker-assisted selection of this gene.

scholarly journals Differential and comparative screening of cowpea varieties to Striga gesnerioides (Willd.) Vatke for race specific identification in Burkina Faso

1970 ◽  
Vol 29 (1) ◽  
pp. 101-118
Author(s):  
P. Sawadogo ◽  
T.J. Ouedraogo ◽  
Z. Dieni ◽  
T.B.J. Batieno ◽  
N. Sawadogo ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Intraspecific Variability ◽  
Intraspecific Diversity ◽  
Research Station ◽  
Striga Gesnerioides ◽  
Resistant Varieties ◽  
Artificial Infestation ◽  
Cowpea Varieties ◽  
Specific Reactions

Significant efforts have been made to develop cowpea (Vigna unguiculata (L.) Walp.) varieties resistant to Striga gesnerioides in Burkina Faso. Despite these efforts, the resistant genotypes developed still express differential responses to Striga gesnerioides in different zones of the country. This suggests existence of intraspecific variability within the parasite. The objective of this study was to assess the intraspecific variability of cowpea genotypes to Striga gesnerioides infection in Burkina Faso. Ten cowpea varieties were screened over two consecutive years, under artificial infestation with 30 ecotypes of Striga seeds at Kamboinsé research station, in a screenhouse in Burkina Faso. Cowpea varieties used included Moussa local, Komsaré and KVx404-8-1, which are susceptible to all Striga ecotypes; and varieties B301, IT93K-693-2 and IT82D-849, which are free from all Striga ecotypes infestation. Cowpea varieties Tiligré, 524B, local Gorom and Niizwè had specific reactions depending on the ecotypes. The study highlights the existence of varietal specificities according to the geographical origin of Striga gesnerioides seeds. The structuring of the intraspecific diversity showed five biotypes, of which three were clearly identified as SG1, SG5 and SG Kp races; and two biotypes could not be identified. Although this study did not allow for a clear determination of the racial affiliation of the two new biotypes, it offers the possibility of developing new strategies to control Striga by focusing on the selection of resistant varieties based on regional specificities of Striga races in each agricultural zone.

scholarly journals RFLP-based Analysis of Recombination Among Resistance Genes to Fusarium Wilt

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 868E-869
Author(s):  
John W. Scott* ◽  
Hesham A. Agrama ◽  
John P. Jones
Keyword(s):  
Fusarium Wilt ◽  
Resistance Genes ◽  
Dominant Gene ◽  
Chromosome 7 ◽  
Rflp Markers ◽  
Single Dominant Gene ◽  
Chromosome 11 ◽  
Intermediate Resistance ◽  
Race 1 ◽  
Race 2

Tomato (Lycopersicon esculentum) line E427 has resistance genes to three races of Fusarium oxysporum f.sp. lycopersici derived from L. pennellii (L.pen) accession LA 716 and L. pimpinellifolium (L.pimp) accession PI 126915. E427 was crossed to susc. Bonny Best and F2 and backcross seed were obtained. Progeny were inoculated separately with Fusarium wilt races 1, 2, or 3. Lines with suspected recombination of resistance were selfed and re-inoculated until disease reactions were homozygous. Four lines were obtained with resistance to both races 2 and 3, but susceptible to race 1. These lines had the L.pen alleles at RFLP markers linked to I-3 on chromosome 7 and lacked L.pimp alleles linked to I and I-2 on chromosome 11. Complementation (F2) data indicated race 2 resistance on chromosome 7 was controlled by a single dominant gene. Three lines were resistant to race 2, but susceptible to races 1 and 3. These lines had L.pimp alleles at TG105 indicating the presence of I-2, and no L.pen alleles at markers linked to I-3. Three lines were resistant to race 1, but susceptible to races 2 and 3. All three had L.pimp alleles at TG523 confirming linkage to I on chromosome 11 and no L.pen alleles at markers tightly linked to I-3. However, one of the lines had L.pen alleles at CT113 on chromosome 7. This and F2 complementation data suggests the possible location of a race 1 resistant locus, I1. Two lines that were Fusarium wilt race 3 resistant and susceptible to race 1 had intermediate resistance to race 2. These two lines did not have the L. pennellii alleles at TG183, TG174, and CT43 near the I-3 locus indicating crossovers in this region reduced race 2 resistance.

scholarly journals A novel bacterial blight resistance gene from Oryza nivara mapped to 38 kb region on chromosome 4L and transferred to Oryza sativa L.

2008 ◽  
Vol 90 (5) ◽  
pp. 397-407 ◽  
Author(s):  
KULJIT K. CHEEMA ◽  
NAVJIT K. GREWAL ◽  
YOGESH VIKAL ◽  
RAJIV SHARMA ◽  
JAGJEET S. LORE ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Resistance Gene ◽  
Ssr Markers ◽  
Resistance Genes ◽  
Bacterial Blight ◽  
Oryza Sativa L ◽  
Bulked Segregant Analysis ◽  
Durable Resistance ◽  
Dominant Gene ◽  
Oryza Nivara

SummaryBacterial blight (BB) of rice caused by Xanthomonas oryzae pv oryzae (Xoo) is one of the major constraints to productivity in South-East Asia. The strategy of using major genes, singly or in combination, continues to be the most effective approach for BB management. Currently, more than two dozen genes have been designated but not all the known genes are effective against all the prevalent pathotypes. The challenge, therefore, is to continue to expand the gene pool of effective and potentially durable resistance genes. Wild species constitute an important reservoir of the resistance genes including BB. An accession of Oryza nivara (IRGC 81825) was found to be resistant to all the seven Xoo pathotypes prevalent in northern states of India. Inheritance and mapping of resistance in O. nivara was studied by using F2, BC2F2, BC3F1 and BC3F2 progenies of the cross involving Oryza sativa cv PR114 and the O. nivara acc. 81825 using the most virulent Xoo pathotype. Genetic analysis of the segregating progenies revealed that the BB resistance in O. nivara was conditioned by a single dominant gene. Bulked segregant analysis (BSA) of F2 population using 191 polymorphic SSR markers identified a ∼35 centiMorgans (cM) chromosomal region on 4L, bracketed by RM317 and RM562, to be associated with BB resistance. Screening of BC3F1 and BC2F2 progenies and their genotyping with more than 30 polymorphic SSR markers in the region, covering Bacterial artificial chromosome (BAC) clone OSJNBb0085C12, led to mapping of the resistance gene between the STS markers based on annotated genes LOC_Os04g53060 and LOC_Os04g53120, which is ∼38·4 kb. Since none of the known Xa genes, which are mapped on chromosome 4L, are effective against the Xoo pathotypes tested, the BB resistance gene identified and transferred from O. nivara is novel and is tentatively designated as Xa30(t). Homozygous resistant BC3F3 progenies with smallest introgression region have been identified.

Resistance gene analogues are clustered on chromosome 3 of sugar beet and cosegregate with QTL for rhizomania resistance

Genome ◽  
2007 ◽  
Vol 50 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Jens Christoph Lein ◽  
Katrin Asbach ◽  
Yanyan Tian ◽  
Daniela Schulte ◽  
Chunyan Li ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Sugar Beet ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Chromosome 3 ◽  
Resistance Locus ◽  
Resistant Varieties ◽  
Rhizomania Resistance

Worldwide, rhizomania is the most important disease of sugar beet. The only way to control this disease is to use resistant varieties. Four full-length resistance gene analogues (RGAs) from sugar beet (cZR-1, cZR-3, cZR-7, and cZR-9) were used in this study. Their predicted polypeptides carry typical nucleotide-binding sites (NBSs) and leucin-rich repeat (LRR) regions, and share high homology to various plant virus resistance genes. Their corresponding alleles were cloned and sequenced from a rhizomania resistant genotype. The 4 RGAs were mapped as molecular markers, using sequence-specific primers to determine their linkage to the rhizomania resistance locus Rz1 in a population segregating for rhizomania resistance. One cZR-3 allele, named Rz-C, together with 5 other molecular markers, mapped to the Rz1 locus on chromosome 3 and cosegregated with quantitative trait loci for rhizomania resistance. After screening a bacterial artificial chromosome (BAC) library, 25 cZR-3-positive BACs were identified. Of these, 15 mapped within an interval of approximately 14 cM on chromosome 3, in clusters close to the Rz1 locus. Rz-C differentiates between susceptible and resistant beet varieties, and its transcripts could be detected in all rhizomania resistant varieties investigated. The potential of this RGA marker for cloning of rhizomania resistance genes is discussed.

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