Sublethal Concentration of Pyriproxyfen Reduces Testicular Connective Tissue Thickness in Euschistus heros Fabr. (Hemiptera: Pentatomidae)

The aim of this study was to determine the effect of the insecticide pyriproxyfen (Tiger™ 100 CE), juvenile hormone analogue, when applied in a sublethal LC30 concentration (0.668 mL a. i. L-1), on the morphological and morphometric parameters of external and internal connective tissue (CT) of the Neotropical-brown stink bug Euschistus heros testicles. The insecticide was applied on nymphs from the 4th instar using a Potter tower with a working pressure of 82.73 kPa (12 lb pol-²) and 1 mL of the emulsion per replicate. A completely randomized experimental design was used, consisting of two treatments (control and pyriproxyfen-treated), five repetitions, and 10 adults of E. heros per experimental unit. The insects were maintained under controlled conditions until the emergence of adults. After 48 h of emergence of adults, the testicles were collected, fixed, and processed for morphological and morphometric analyses. A change was observed in the collagen fibers of the CT of treated insects when compared with those of controls. It was also observed that both types of CT (dense irregular and loose) over the internal tunic of the treated insect were thinner than those in control insects. The analysis showed that pyriproxyfen significantly reduced the external and internal CT width and the conformation of its fibers in all the observed regions when compared with the controls; this may affect the production of the three different types of sperm present in this species.

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Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n4suplp2279 ◽

2017 ◽

Vol 38 (4Supl1) ◽

pp. 2279 ◽

Cited By ~ 1

Author(s):

Paulo Sérgio Gimenez Cremonez ◽

Daniela De Oliveira Pinheiro ◽

Ângela Maria Ferreira Falleiros ◽

Pedro Manuel Oliveira Janeiro Neves

Keyword(s):

Reproductive System ◽

Morphological Changes ◽

Sublethal Concentration ◽

Experimental Unit ◽

Stink Bugs ◽

Female Fecundity ◽

Insect Growth Regulators ◽

Morphological Alterations ◽

Randomized Experimental Design ◽

Adult Sex Ratio

The use of insecticides known as insect growth regulators, which are considered more selective to natural enemies, may be an alternative to integrated pest management of stink bugs of the main crops in Brazil. The objective of this study was to evaluate histological changes in the reproductive system of Dichelops melacanthus as well as female fecundity and egg fertility after use of growth-regulating insecticides. The insecticides used were buprofezin (a chitin biosynthesis inhibitor) at a sublethal concentration (LC30 of 2.99 g L-1) and pyriproxyfen (a juvenile hormone analog) at a sublethal concentration (LC30 of 8.35 mL L-1). A completely randomized experimental design was used, with 10 repetitions and 10 insects per experimental unit. Two bioassays were performed: in the first bioassay, fecundity (eggs/female) and fertility (nymph eclosion) of the insects that survived the insecticides were evaluated; in the second bioassay, morphological alterations of the ovarioles of adult females and of the testes of adult male insects that survived the insecticides were evaluated. Buprofezin and pyriproxyfen did not affect the adult sex ratio or female fecundity. Pyriproxyfen reduced the percentage of ecloded nymphs (71.6%) compared with that of the control and buprofezin (96.4 and 90.6%, respectively) treatments and had an ovicidal effect, with direct and indirect action on embryogenesis. Morphological changes were observed in both treatments with buprofezin and pyriproxyfen. The alterations observed in female and male reproductive systems may occur by the action of buprofezin and pyriproxyfen on the morphology of both ovarioles and testes.

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Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n4supl1p2279 ◽

2017 ◽

Vol 38 (4Supl1) ◽

pp. 2279

Author(s):

Paulo Sérgio Gimenez Cremonez ◽

Daniela De Oliveira Pinheiro ◽

Ângela Maria Ferreira Falleiros ◽

Pedro Manuel Oliveira Janeiro Neves

Keyword(s):

Reproductive System ◽

Morphological Changes ◽

Sublethal Concentration ◽

Experimental Unit ◽

Stink Bugs ◽

Female Fecundity ◽

Insect Growth Regulators ◽

Morphological Alterations ◽

Randomized Experimental Design ◽

Adult Sex Ratio

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Mode of intoxication of the neotropical brown stink bug,Euschistus heros, in soybeans treated with insecticides

10.1603/ice.2016.115566 ◽

2016 ◽

Author(s):

Geraldo Papa

Keyword(s):

Stink Bug ◽

Euschistus Heros

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A Closer Look at the Cellular and Molecular Components of the Deep/Muscular Fasciae

International Journal of Molecular Sciences ◽

10.3390/ijms22031411 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1411

Author(s):

Caterina Fede ◽

Carmelo Pirri ◽

Chenglei Fan ◽

Lucia Petrelli ◽

Diego Guidolin ◽

...

Keyword(s):

Extracellular Matrix ◽

Connective Tissue ◽

Treatment Strategies ◽

Clear Understanding ◽

Pathological Characteristics ◽

Appropriate Treatment ◽

Different Types ◽

Molecular Components ◽

Entire Network ◽

Shed Light

The fascia can be defined as a dynamic highly complex connective tissue network composed of different types of cells embedded in the extracellular matrix and nervous fibers: each component plays a specific role in the fascial system changing and responding to stimuli in different ways. This review intends to discuss the various components of the fascia and their specific roles; this will be carried out in the effort to shed light on the mechanisms by which they affect the entire network and all body systems. A clear understanding of fascial anatomy from a microscopic viewpoint can further elucidate its physiological and pathological characteristics and facilitate the identification of appropriate treatment strategies.

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Diapause and different seasonal morphs of Euschistus heros (Fabr.) (Hemiptera: Pentatomidae) in Northern Paraná State

Anais da Sociedade Entomológica do Brasil ◽

10.1590/s0301-80592000000200002 ◽

2000 ◽

Vol 29 (2) ◽

pp. 205-218 ◽

Cited By ~ 12

Author(s):

Ana P. M. Mourão ◽

Antônio R. Panizzi

Keyword(s):

Mangifera Indica ◽

Reproductive Organs ◽

Stink Bug ◽

Ligustrum Lucidum ◽

Mango Tree ◽

Euschistus Heros ◽

Males And Females ◽

Fallen Leaves ◽

Diapause Incidence ◽

In The Beginning

The Neotropical brown stink bug, Euschistus heros (Fabr.), was collected on sunflower [Helianthus annuus (L.)], soybean [Glycine max (L.) Merrill], star bristle [Acanthospermum hispidum (DC.)], and under fallen leaves of mango tree [Mangifera indica (L.)] and privet (Ligustrum lucidum Ait.), during one year, to evaluate the diapause incidence and the occurrence of different seasonal morphs. The majority of the insects (ca. 90%) with mature reproductive organs was observed during the summer (December-March), when E. heros was found on soybean or on sunflower; in the beginning of autumn, most insects (87%) showed immature reproductive organs, and they were found on star bristle and under fallen leaves. Bugs with mature reproductive organs had more developed shoulders (3.23 and 3.27 mm, for males and females, respectively) than bugs with immature organs (2.91 and 2.89 mm, for males and females, respectively). Two distinct body colors, dark brown and reddish brown, were observed. Nevertheless, the reddish brown was the predominant color of both mature and immature adults during all year. Adults were parasitized by Hexacladia smithii (Ashmead) (Hymenoptera: Encyrtidae) and Tachinidae mainly Trichopoda giacomellii (Blanchard) (Diptera: Tachinidae) (80% and 40% of parasitism in male and female, respectively) during summer (December), when the bugs colonized sunflower. In soybean, 12% of males and 10% of females were parasitized, whereas in fallen leaves, the parasitism rate was 5% in both sexes.These results showed that in autumn/winter (shorter photoperiod) the majority of the insects were inactive under fallen leaves, showing immature reproductive organs and less developed shoulders, indicating that, at this time, these bugs were in diapause.

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EXPRESSION OF FACTOR XIII SUBUNIT A IN DIFFERENT TYPES OF HUMAN MACROPHAGES

10.1055/s-0038-1644651 ◽

1987 ◽

Author(s):

R Adany ◽

A Kiss ◽

J Kappelmayer ◽

R J Ablin ◽

L Muszbek

Keyword(s):

Bone Marrow ◽

Connective Tissue ◽

Cell Line ◽

Factor Xiii ◽

Bone Cells ◽

Human Peripheral Blood ◽

Coagulation Factor ◽

Macrophage Cell ◽

Subunit A ◽

Different Types

In addition to plasma the presence of subunit a of blood coagulation Factor XIII (FXIIl) has been verified in platelets and megakariocytes. Most recently, we demonstrated that human peripheral blood monocytes also contain FXIII subunit a. The present study was designed 1/ to determine the stage in the maturation sequence of bone marrow monocytopoesis in which FXIII appears 2/ to establish if FXIII is retained during differentiation into macrophages 3/ to assess how general is the presence of FXIII subunit a in different types of macrophages. FXIII subunit a was immunomorphologically detected in bone marrow smears, in cytospin preparations of cells from serous cavities (pleural, peritoneal, pericardial and synovial spaces), and paraformaldehyde-fixed paraffin-embedded or frozen sections of different organs where classical types of macrophages have been described earlier (liver, lung, thymus, skin, connective tissue, prostate and developing bone) . Cells containing FXIII subunit a were intensively characterized by immunofluorescent and enzymecytochemical techniques in double and treble labeling systems. Its presence was clearly demonstrated in promonocytes of bone marrow, and in all probability, it is present in monoblasts, as well. FXIII was also found in macrophages from different serous cavities and in embryonic osteoclasts. Cells containing FXIII subunit a of connective tissue were found to be tissue histiocytes, and not fibroblasts as previously thought. Kupffer cells of the liver and Langerhans cells of the epidermis were negative supporting theories that these cells are not members of monocyte-derived macrophage cell population. Immunomorphological detection of FXIII subunit a seems to be a useful marker for labeling the continuum of monocyte/macrophage cell line from the earliest ftrais in the bone marrow to the mature forms of macrophages and might be a valuable tool in the cytological diagnosis of malignant disorders of this cell line.

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Parâmetros biológicos de Euschistus heros (Fabricius, 1794) alimentado com diferentes dietas

Revista Agraria Academica ◽

10.32406/v4n12021/152-159/agrariacad ◽

2021 ◽

Vol 4 (1) ◽

pp. 152-159

Author(s):

Yves Pierre Harry Dalleinne ◽

◽

Aline Giothi ◽

Rosane Betina Wandscheer ◽

Ivonete Hoss ◽

...

Keyword(s):

Food Preference ◽

Eating Habits ◽

Feeding Preference ◽

Stink Bug ◽

Euschistus Heros ◽

Males And Females ◽

Seeds And Fruits

During their development, insects can suck plant structures like seeds and fruits being the preferred structures. The study aims to monitor the eating habits of these insects are relevant mainly in terms of food preference and development. The objective of this work was to analyze the feeding preference of Euschistus heros (brown stink bug) fed with 5 diets. The different diets studied resulted in insects in adult, with significant differences in the length of the nymph period, differences in the weights of males and females and variation in survival during the experiment.

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Pentatomids associated with blackberry

Ciência e Agrotecnologia ◽

10.1590/s1413-70542014000300005 ◽

2014 ◽

Vol 38 (3) ◽

pp. 256-261

Author(s):

Mauricio Paulo Batistella Pasini ◽

Alessandro Dal'Col Lúcio

Keyword(s):

Rio Grande ◽

Nezara Viridula ◽

Brown Spot ◽

Stink Bug ◽

Stink Bugs ◽

Rio Grande Do Sul ◽

Dark Brown Spot ◽

Euschistus Heros ◽

Santa Maria ◽

Piezodorus Guildinii

Information concerning the presence of stink bugs in blackberry (Rubus spp.) in Brazil is sparse. This study aimed to identify the stink bug species associated with blackberry, to establish the daily dynamics and evaluate the fruits damage. The experiment was conducted in Santa Maria, Rio Grande do Sul State, Brazil, in blackberry orchard. Presence and evaluations of stink bugs were done weekly through visual and sweeping samplings at different day times. Five species of pentatomids were identified: Piezodorus guildinii, Nezara viridula, Euschistus heros, Dichelops furcatus and Edessa meditabunda. The bugs attack the drupelets producing a dark brown spot and wrinkled berries.

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A Simple Flexible Plate for Usage as Force Sensor Element With Constant Stiffness

Volume 4A: Dynamics, Vibration and Control ◽

10.1115/imece2013-63571 ◽

2013 ◽

Author(s):

Thomas Ottnad ◽

Henrik Lüßmann ◽

Tim C. Lueth

Keyword(s):

Pressure Sensor ◽

Pressure Sensors ◽

Force Sensor ◽

Mechanical Deformation ◽

Test Rig ◽

Working Pressure ◽

Constant Stiffness ◽

Flexible Element ◽

Electrical Actuator ◽

Different Types

Control of pressure is one of the most decisive parameters influencing the quality and reproducibility of products when dealing with plastics producing techniques such as injection molding, extrusion, and related techniques. For that purpose different types of pressure sensors for specific tasks come into action. Different tasks can be different working pressure ranges, static or dynamic pressure, working temperatures and requirements concerning the environment. Therefore different types of pressure sensors are commonly used which most often transform mechanical deformation into electrical signals and these in turn are converted into the value of pressure. This in turn can mean extensive effort for installation and insulation of the pressure sensors. An approach to design a pressure sensor based solely on its mechanical deformation is basic idea of the present work. The pressure sensor consists of a flexible element which is in fact a thin plate with a ram on its outer side and can be clamped easily at any desired area on a melt channel. When being applied to pressure the working pressure causes a deflection of the flexible element. Measuring this deflection and knowing the mechanical behavior of the flexible element allows the calculation of the value of pressure. To get to know to the mechanical behavior, especially the stiffness, of the flexible element a test rig was designed and refined. In the test rig as substitution of the pressure variable but defined load can be applied using a piezo-electrical actuator with integrated position sensor. Measuring the applied force using a force sensor and measuring the resulting deflection of the flexible element using a length gauge allows to calculate the stiffness of the flexible element. Regarding the flexible element as Kirchhoff plate a constant stiffness is expected and this could be proven taking measurements. When taking a look at the deflection of the piezo-electrical actuator and the flexible element there is a difference due to the elasticity of the test rig itself. When determining the stiffness of the test rig a non-constant stiffness was detected which was not expected. Reason for that is the non-constant stiffness of the used force sensor though stated other by the manufacturer. This could be proven substituting the force sensor with the flexible element. With that the flexible element showed to be appropriate to be used as force sensor with a constant stiffness.

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