scholarly journals Serum HDL2-and HDL3-Cholesterol Levels in Normal Subjects and Patients with Various Diseases by High Performance Liquid Chromatography (HPLC)

1981 ◽  
Vol 9 (2) ◽  
pp. 157-161
Author(s):  
Akira TANAKA ◽  
Isao UCHIMURA ◽  
Hidenori MAEZAWA ◽  
Mitsuyo OKAZAKI ◽  
Yoshimi OHNO ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Normal Subjects ◽  
Cholesterol Levels

Neutrophil-activating peptide-2 in patients with pulmonary edema from congestive heart failure or ARDS

1993 ◽  
Vol 264 (5) ◽  
pp. L490-L495 ◽  
Author(s):  
A. B. Cohen ◽  
M. D. Stevens ◽  
E. J. Miller ◽  
M. A. Atkinson ◽  
G. Mullenbach ◽  
...  
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pulmonary Edema ◽  
High Performance ◽  
Distress Syndrome ◽  
Normal Subjects ◽  
Antigen Concentration ◽  
Fluid Formation

We carried out studies to determine whether the neutrophil-activation peptide-2 (NAP-2) plays a role in the recruitment and/or degranulation of neutrophils into the lungs of patients with the adult respiratory distress syndrome (ARDS) or congestive heart failure (CHF). NAP-2 precursors plus NAP-2 (beta-thromboglobulin-like antigen) were measured in lung fluids and plasmas with a radioimmunoassay, and NAP-2 was separated from its precursors by high-performance liquid chromatography. Pulmonary edema fluids (PEFs) from patients with CHF contained higher concentrations of the beta-thromboglobulin-like antigen than PEFs from patients with ARDS, and bronchoalveolar lavage fluids (BALs) from patients with ARDS contained higher concentrations of beta-thromboglobulin-like antigen than BALs from normal subjects. beta-Thromboglobulin-like antigen concentration was 4.1-fold greater in PEFs from patients with CHF than in their plasmas. Chemotactically active NAP-2 was also demonstrated in PEFs but not in plasmas from patients with CHF and ARDS. These data suggest that significant platelet degranulation occurred into the lungs of the patients with CHF and that NAP-2 and other platelet constituents may contribute to fluid formation in patients with CHF.

Liquid-chromatographic profiling of endogenous fluorescent substances in sera and urine of uremic and normal subjects.

1983 ◽  
Vol 29 (4) ◽  
pp. 675-677 ◽  
Author(s):  
H Mabuchi ◽  
H Nakahashi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Normal Subjects ◽  
Body Fluids ◽  
Normal Serum ◽  
Fluorescence Excitation ◽  
Normal Urine ◽  
Liquid Chromatographic ◽  
Chromatographic Profiling

Abstract Endogenous fluorescent substances increase in serum during uremia. We have used "high-performance" liquid chromatography to profile these fluorescent substances in both uremic and normal body fluids. The fluorescence excitation and emission maxima we used were 322 and 415 nm, respectively. Of the numerous fluorescent substances found in uremic body fluids and in normal urine, some were also detectable in normal serum, but at relatively weak fluorescence intensities.

Characterization by high-performance liquid chromatography of circulating growth hormone-releasing factors in normal subjects

1986 ◽  
Vol 111 (3) ◽  
pp. 507-511 ◽  
Author(s):  
E. S. Penny ◽  
A. M. Sopwith ◽  
R. L. Patience ◽  
J. A. H. Wass ◽  
G. M. Besser ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Large Scale ◽  
Molecular Form ◽  
Normal Subjects ◽  
Reversed Phase ◽  
Percentage Increase ◽  
Mixed Meal ◽  
Analytical Separation

ABSTRACT Four forms of circulating immunoreactive human GH-releasing factor (ir-hGRF) have been identified in each of four normal subjects, with a mean increase in total ir-hGRF of twofold over basal levels following a mixed meal. Plasma samples (200 ml) from each individual were subjected to large-scale Vycor extraction with initial purification by high-performance liquid chromatography on a reversed-phase C18 column, followed by analytical separation of the ir-hGRF components using a C3 wide-pore reversed-phase column, and subsequent radioimmunoassay of the fractions. The mean recovery of total ir-hGRF from the plasma (fasted and non-fasted) was 76±16% (2×s.e.m.). Analytical separation of the ir-hGRF revealed four components which co-eluted with synthetic hGRF-37, hGRF-40 and hGRF-44, and a peak eluting between hGRF-40 and -44 which may represent hGRF-42. The hGRF-40 was shown to be the predominant circulating molecular form in the fasted state in each subject, and in three out of four subjects following a mixed meal. The hGRF-44 showed the greatest percentage increase over basal in all four individuals. J. Endocr. (1986) 111, 507–511

scholarly journals Determination of L-tryptophan and L-kynurenine in Human Serum by using LC-MS after Derivatization with (R)-DBD-PyNCS

2013 ◽  
Vol 6s1 ◽  
pp. IJTR.S11459 ◽  
Author(s):  
Hayato Ohashi ◽  
Hideaki Iizuka ◽  
Shunsuke Yoshihara ◽  
Hayato Otani ◽  
Misato Kume ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Significant Positive Correlation ◽  
Normal Subjects ◽  
Mass Spectrometric ◽  
Male And Female ◽  
Male Subjects ◽  
Female Subjects

Concentrations of L-tryptophan (L-Trp) and its metabolite, L-kynurenine (L-KYN), in sera of 19 normal subjects (age: 23.6 ± 3.5 y, male: 8, female: 11) were determined by high-performance liquid chromatography with mass-spectrometric detection, following their derivatization with ( R)-(–)-4-(N, N-dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole (DBD-PyNCS). A significant positive correlation between L-Trp and L-KYN concentrations was observed (r = 0.532, P < 0.05). Serum L-Trp concentration in male subjects (95.65 ± 4.27 μM) was significantly higher than that in female subjects (79.20 ± 3.34 μM; P < 0.05), while no significant differences in L-KYN concentration or the L-KYN:L-Trp ratio were observed between male and female subjects.

Dopamine and Its Metabolites in Human Tears

1993 ◽  
Vol 3 (2) ◽  
pp. 83-88 ◽  
Author(s):  
X.D. Martin ◽  
M.C. Brennan
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ion Transport ◽  
Corneal Epithelium ◽  
High Performance ◽  
Homovanillic Acid ◽  
Normal Subjects ◽  
Trigeminal Stimulation ◽  
The Mean ◽  
Mean Concentration

Dopamine is known to stimulate ion transport in the corneal epithelium. Since epithelium is in contact with tears, we searched for dopamine and its metabolites in tears. Twenty normal subjects participated in this study. Twenty μl of tears were collected in capillary tubes after trigeminal stimulation and immediately analyzed using high-performance liquid chromatography with electrochemical detection. Dopamine and two of its metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), were identified and measured. The mean concentration of dopamine was 8.9 ±5.1 ng/ml; DOPAC, 2.2 ± 1.6 ng/ml; and HVA, 0.55 ± 0.4 ng/ml. These results permit us to suggest that dopamine is deaminated by monoamine oxidase, then methylated by catechol-O-methyltransferase and that catabolism occurs in the lacrimal gland and perhaps in corneal epithelium. Finally, part of the dopamine involved in ion transport may come from tears.

Sign in / Sign up

Export Citation Format

Share Document