scholarly journals Investigation of dissolved N2O production processes during wastewater treatment system in Ulaanbaatar

2017 ◽  
Vol 17 (43) ◽  
pp. 23-27
Author(s):  
Tumendelger A ◽  
Byambadorj T ◽  
C Bors ◽  
A Lorke
Keyword(s):  
Wastewater Treatment ◽  
Inorganic Nitrogen ◽  
Stratospheric Ozone ◽  
Treatment Plant ◽  
Nitrifying Bacteria ◽  
Aeration Tank ◽  
Site Preference ◽  
N2o Production ◽  
Biological Reaction ◽  
No2 Reduction

Nitrous oxide (N2O) is an increasing greenhouse gas in the troposphere and a potential destroyer of stratospheric ozone layer. Wastewater treatment plant (WWTP) is one of the anthropogenic N2O sources because inorganic and organic nitrogen compounds are converted to nitrate (NO3-, in the case of standard system) or N2 (in the case of advanced system) by bacterial nitrification and denitrifcation processes in WWTP. These major processes can be distinguished by isotopocule analysis. In order to reveal production mechanisms of N2O in a standard wastewater treatment, we made water sampling at the central WWTP in Ulaanbaatar. The water samples collected from seven stations including biological reaction tanks were measured for concentration and isotopocule ratios of dissolved N2O and other inorganic nitrogen. Dissolved N2O concentration was extremely higher than that expected under atmospheric equilibrium (about 9 nmol/l) at all stations, indicating that this system is a potential source of N2O. It showed a gradual increase with the progress of biological reaction and the highest concentration (335.7 nmol/l) was observed at station N5-4 of the aeration tank when the DO was 5.7 mg/l. Nitrification by nitrifying bacteria could actively occur by the concentration of NH4+ decreased whereas NO2- and NO3- showed a temporal and monotonic increase, respectively, under high DO concentration. Although the reported values of site preference (SP) of N2O, the difference in 15N/14N ratio between central (α) and terminal (β) nitrogen, produced via NO2- reduction (SP(ND)), including both nitrifier and denitrifier denitrification, and NH2OH oxidation (SP(HO)) ranged from -10.7‰ to 0‰ and 31.4‰ to 36.3‰, respectively, the observed SP at aeration tank was close to SP(ND) rather than SP(HO). It was ranged from 0.4‰ to 13.3‰ when N2O concentration was high, implying that the NO2- reduction made a greater contribution to N2O production. Slightly elevated SP (13.3‰) only at station N5-1 was derived from the mixing of N2O produced via NH2OH oxidation and the maximal contribution of this pathway was estimated to be about 40%. In other words, the contribution of NO2- reduction was more than 60%.

scholarly journals Biogeochemical controls and isotopic signatures of nitrous oxide production by a marine ammonia-oxidizing bacterium

2010 ◽  
Vol 7 (9) ◽  
pp. 2695-2709 ◽  
Author(s):  
C. H. Frame ◽  
K. L. Casciotti
Keyword(s):  
Nitrous Oxide ◽  
Stratospheric Ozone ◽  
Nitrifying Bacteria ◽  
Site Preference ◽  
Ammonia Oxidizing Bacteria ◽  
N2o Production ◽  
O2 Concentration ◽  
Cell Densities ◽  
Dissolved O2

Abstract. Nitrous oxide (N2O) is a trace gas that contributes to the greenhouse effect and stratospheric ozone depletion. The N2O yield from nitrification (moles N2O-N produced per mole ammonium-N consumed) has been used to estimate marine N2O production rates from measured nitrification rates and global estimates of oceanic export production. However, the N2O yield from nitrification is not constant. Previous culture-based measurements indicate that N2O yield increases as oxygen (O2) concentration decreases and as nitrite (NO2−) concentration increases. Here, we have measured yields of N2O from cultures of the marine β-proteobacterium Nitrosomonas marina C-113a as they grew on low-ammonium (50 μM) media. These yields, which were typically between 4 × 10−4 and 7 × 10−4 for cultures with cell densities between 2 × 102 and 2.1 × 104 cells ml−1, were lower than previous reports for ammonia-oxidizing bacteria. The observed impact of O2 concentration on yield was also smaller than previously reported under all conditions except at high starting cell densities (1.5 × 106 cells ml−1), where 160-fold higher yields were observed at 0.5% O2 (5.1 μM dissolved O2) compared with 20% O2 (203 μM dissolved O2). At lower cell densities (2 × 102 and 2.1 × 104 cells ml−1), cultures grown under 0.5% O2 had yields that were only 1.25- to 1.73-fold higher than cultures grown under 20% O2. Thus, previously reported many-fold increases in N2O yield with dropping O2 could be reproduced only at cell densities that far exceeded those of ammonia oxidizers in the ocean. The presence of excess NO2− (up to 1 mM) in the growth medium also increased N2O yields by an average of 70% to 87% depending on O2 concentration. We made stable isotopic measurements on N2O from these cultures to identify the biochemical mechanisms behind variations in N2O yield. Based on measurements of δ15Nbulk, site preference (SP = δ15Nα−δ15Nβ), and δ18O of N2O (δ18O-N2O), we estimate that nitrifier-denitrification produced between 11% and 26% of N2O from cultures grown under 20% O2 and 43% to 87% under 0.5% O2. We also demonstrate that a positive correlation between SP and δ18O-N2O is expected when nitrifying bacteria produce N2O. A positive relationship between SP and δ18O-N2O has been observed in environmental N2O datasets, but until now, explanations for the observation invoked only denitrification. Such interpretations may overestimate the role of heterotrophic denitrification and underestimate the role of ammonia oxidation in environmental N2O production.

Recycling of water treatment sludges in municipal wastewater treatment plants – a practical example

2008 ◽  
Vol 3 (1) ◽  
Author(s):  
K. Klinksieg ◽  
T. Dockhorn ◽  
N. Dichtl
Keyword(s):  
Wastewater Treatment ◽  
Water Treatment ◽  
Wastewater Treatment Plant ◽  
Iron Hydroxide ◽  
Settling Tank ◽  
Treatment Plant ◽  
Full Scale ◽  
Aeration Tank ◽  
Second Phase ◽  
Precipitation Efficiency

Full-scale and lab-scale research experiments were conducted to determine the phosphorous precipitation efficiency of iron hydroxide sludge from drinking water treatment. During full-scale investigations at a wastewater treatment plant, ferric sludge was added to the inflow of the primary settling tank in a first experimental phase and to the inflow of the aeration tank in a second phase. In the outflow of the mechanical stage and in the outflow of the biological stage, a reduction of the PO4-P concentrations could be observed. The concentration of COD, the SVI and the filament abundance were not changed significantly by adding the ferric sludge to the wastewater treatment plant. In lab tests, improved precipitation efficiency of the ferric sludge could be achieved by using anaerobic conditions and acid pulping. The research showed that the wastewater treatment process can benefit from the reuse of ferric sludge from drinking waterworks and that this also presents an inexpensive recycling option for these sludges.

scholarly journals Assessment of wastewater treatment plant effluent impact on the ecosystem of the river on the basis of the quantitative development of ciliated protozoa characteristic of the aeration tank

2020 ◽  
Author(s):  
R. Babko ◽  
V. Pliashechnyk ◽  
T. Kuzmina ◽  
Y. Danko ◽  
J. Szulżyk-Cieplak ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Waste Treatment ◽  
Treatment Plant ◽  
Aeration Tank ◽  
Quantitative Characteristics ◽  
Quantitative Development ◽  
Treatment Facilities ◽  
The Impact

Abstract The work is devoted to the task of simplifying the assessment of the effect of effluents from treatment facilities on the river hydrobiocenosis. The studies were carried out on the mountain river Uzh (Uzhgorod, Ukraine). Our approach to assessing the impact of waste treatment facilities on the river receiver is based on the estimate of the similarity of species composition and quantitative characteristics of populations of organisms from the aerotank and from the river. It is shown that the quantitative development of populations of species of ciliates from the aeration tank is a good indicator for assessing the degradation of organic matter coming with wastewater. The use of qualitative and quantitative characteristics of the protozoa from the wastewater treatment plant as a criterion for assessing the quality of the environment in the area of wastewater discharge showed their representativeness and effectiveness. The use of a limited number of species makes it possible to conduct an express assessment of the effect of effluents on receiving reservoirs for specialists working with activated sludge in the laboratories of treatment facilities.

scholarly journals Air concentrations and particle–gas partitioning of polyfluoroalkyl compounds at a wastewater treatment plant

2011 ◽  
Vol 8 (4) ◽  
pp. 363 ◽  
Author(s):  
Lena Vierke ◽  
Lutz Ahrens ◽  
Mahiba Shoeib ◽  
Eric J. Reiner ◽  
Rui Guo ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Air Sampling ◽  
Passive Samplers ◽  
Aeration Tank ◽  
Sampling Campaign ◽  
Secondary Clarifier ◽  
Polyfluoroalkyl Compounds ◽  
Air Concentrations

Environmental contextPolyfluoroalkyl compounds, widely used chemicals in consumer and industrial products, are global pollutants in the environment. Transport mechanisms and environmental pathways of these compounds, however, are not yet fully understood. We show that a wastewater treatment plant can be an important source for polyfluoroalkyl compounds to the atmosphere where they have the potential to be transported long distances. AbstractAn air sampling campaign was conducted at a wastewater treatment plant (WWTP) to investigate air concentrations and particle–gas partitioning of polyfluoroalkyl compounds (PFCs). Samples were collected at an aeration tank and a secondary clarifier using both active high volume samplers and passive samplers comprising sorbent-impregnated polyurethane foam (SIP) disks. Water to air transport of PFCs was believed to be enhanced at the aeration tank owing to aerosol-mediated transport caused by surface turbulence induced by aeration. Mean air concentrations of target PFCs at the aeration tank were enriched relative to the secondary clarifier by factors of ~19, ~4 and ~3 for ∑fluorotelomer alcohols (FTOHs) (11 000 v. 590 pg m–3), ∑perfluorooctane sulfonamides & perfluorooctane sulfonamidoethanols (FOSAs & FOSEs) (120 v. 30 pg m–3) and ∑perfluoroalkyl carboxylates & perfluoroalkyl sulfonates (PFCAs & PFSAs) (4000 v. 1300 pg m–3) respectively. The particle associated fraction in the atmosphere increased with increasing chain length for PFCAs (from 60 to 100%) and PFSAs were predominantly bound to particles (~98%). Lower fractions on particles were found for FTOHs (~3%), FOSAs (~30%) and FOSEs (~40%). The comparison of the active and passive air sampling showed good agreement.

scholarly journals Evaluation of the efficiency of selected wastewater treatment plant

2012 ◽  
Vol 60 (1) ◽  
pp. 173-180
Author(s):  
Tomáš Vítěz ◽  
Jana Ševčíková ◽  
Petra Oppeltová
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Suspended Solids ◽  
Inorganic Nitrogen ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Ammonia Nitrogen ◽  
Total Efficiency ◽  
Treatment Efficiency ◽  
Wastewater Treatment Process

This paper is focused on primary, secondary, and total efficiency evaluation of the wastewater treatment process for chosen small wastewater treatment plant (WWTP) located near the Moravian Karst. Eight wastewater samples were taken during one year in three sampling profiles of WWTP: biochemical oxygen demand (BOD), chemical oxygen demand (COD), suspended solids (SS), pH, ammonia nitrogen (N-NH4), nitrite nitrogen (N-NO2), nitrate nitrogen (N-NO3), inorganic nitrogen (Ninorg), total phosphorus (Ptotal). Treatment efficiency by reduction was calculated for all laboratory analyzed indicators and average values were determined for the whole period. Calculated treatment efficiency of indicators BOD, COD and suspended solids was compared with the permissible minimum treatment efficiency of discharged waste water by Government Regulation No. 61/2003 Coll., for the WWTP from 500 to 2 000 PE. Permissible minimum treatment efficiency is not legislatively determined for the primary and secondary level. The results of the work will be used especially to compare results with other similar works.Analyzed values ​​of parameters BOD, COD, suspended solids, N-NH4 at the outflow from wastewater treatment plant were compared with the permissible maximum values at the outflow of the WWTP which the municipality has an obligation to respect according to the decision issued by the District Environment Authority.

scholarly journals Source identification of N2O produced during simulated wastewater treatment under different oxygen conditions using stable isotopic analysis

2014 ◽  
Vol 15 ◽  
pp. 4-10 ◽  
Author(s):  
T Azzaya ◽  
Sakae Toyoda ◽  
Naohiro Yoshida ◽  
Hiroshi Shiomi ◽  
Rina Kouno
Keyword(s):  
Wastewater Treatment ◽  
Isotope Analysis ◽  
Treatment Plant ◽  
Aerobic Condition ◽  
Isotope Ratio Mass Spectrometry ◽  
Nitrogen Isotope ◽  
Isotopic Analysis ◽  
Ammonia Oxidizing Bacteria ◽  
N2o Production ◽  
Nitrification And Denitrification

Nitrous oxide (N2O), a potent greenhouse gas which is important in climate change, is predicted to be the most dominant ozone depleting substance. It is mainly produced by oxidation of hydroxylamine (NH2OH) or reduction of nitrite (NO2-) during microbiological processes such as nitrification and denitrification. Wastewater treatment plant (WWTP) is one of the anthropogenic N2O sources because inorganic and organic nitrogen compounds are converted to nitrate (NO3-, in the case of standard system) or N2 (in the case of advanced system) by bacterial nitrification and denitrification in WWTP. We investigated the N2O production mechanisms during batch experiments that simulate wastewater treatment with activated sludge under various dissolved oxygen (DO) concentrations by stable isotope analysis. About 125mL of water was sampled from 30L incubation chamber for several times during the incubation, and concentration and isotopomer ratios of N2O and N-containing species were measured using gas chromatography/isotope ratio mass spectrometry (GC/IRMS). Ammonium (NH4+) consumption was accompanied by increment of nitrite (NO2-), and at the same time dissolved N2O concentration gradually increased to 4850 and 5650 nmol kg-1, respectively, during the four-hour incubation when DO concentrations were 0.2 and 0.5 mg L-1. Observed low SP values (0.2-8.9‰ at DO-0.2 mg L-1, -5.3-6.3‰ at DO-0.5 mg L-1, -1.0-8.3‰ at DO-0.8 mg L-1) in N2O and relationship of nitrogen isotope ratios between N2O and its potential substrates (NH4+, NO3-) suggested that N2O produced under the aerobic condition derived mainly from NO2- reduction by ammonia-oxidizing bacteria (nitrifier–denitrification).DOI: http://doi.dx.org/10.5564/mjc.v15i0.313Mongolian Journal of Chemistry  15 (41), 2014, p4-10  

scholarly journals Impact of reactive surfaces on the abiotic reaction between nitrite and ferrous iron and associated nitrogen and oxygen isotope dynamics

2020 ◽  
Vol 17 (16) ◽  
pp. 4355-4374
Author(s):  
Anna-Neva Visser ◽  
Scott D. Wankel ◽  
Pascal A. Niklaus ◽  
James M. Byrne ◽  
Andreas A. Kappler ◽  
...  
Keyword(s):  
Isotope Effect ◽  
Ex Vivo ◽  
Reduction Rate ◽  
Site Preference ◽  
Experimental Conditions ◽  
N2o Production ◽  
O Isotope ◽  
No2 Reduction ◽  
The Impact ◽  
Reactive Surfaces

Abstract. Anaerobic nitrate-dependent Fe(II) oxidation (NDFeO) is widespread in various aquatic environments and plays a major role in iron and nitrogen redox dynamics. However, evidence for truly enzymatic, autotrophic NDFeO remains limited, with alternative explanations involving the coupling of heterotrophic denitrification with the abiotic oxidation of structurally bound or aqueous Fe(II) by reactive intermediate nitrogen (N) species (chemodenitrification). The extent to which chemodenitrification is caused (or enhanced) by ex vivo surface catalytic effects has not been directly tested to date. To determine whether the presence of either an Fe(II)-bearing mineral or dead biomass (DB) catalyses chemodenitrification, two different sets of anoxic batch experiments were conducted: 2 mM Fe(II) was added to a low-phosphate medium, resulting in the precipitation of vivianite (Fe3(PO4)2), to which 2 mM nitrite (NO2-) was later added, with or without an autoclaved cell suspension (∼1.96×108 cells mL−1) of Shewanella oneidensis MR-1. Concentrations of nitrite (NO2-), nitrous oxide (N2O), and iron (Fe2+, Fetot) were monitored over time in both set-ups to assess the impact of Fe(II) minerals and/or DB as catalysts of chemodenitrification. In addition, the natural-abundance isotope ratios of NO2- and N2O (δ15N and δ18O) were analysed to constrain the associated isotope effects. Up to 90 % of the Fe(II) was oxidized in the presence of DB, whereas only ∼65 % of the Fe(II) was oxidized under mineral-only conditions, suggesting an overall lower reactivity of the mineral-only set-up. Similarly, the average NO2- reduction rate in the mineral-only experiments (0.004±0.003 mmol L−1 d−1) was much lower than in the experiments with both mineral and DB (0.053±0.013 mmol L−1 d−1), as was N2O production (204.02±60.29 nmol L−1 d−1). The N2O yield per mole NO2- reduced was higher in the mineral-only set-ups (4 %) than in the experiments with DB (1 %), suggesting the catalysis-dependent differential formation of NO. N-NO2- isotope ratio measurements indicated a clear difference between both experimental conditions: in contrast to the marked 15N isotope enrichment during active NO2- reduction (15εNO2=+10.3 ‰) observed in the presence of DB, NO2- loss in the mineral-only experiments exhibited only a small N isotope effect (<+1 ‰). The NO2--O isotope effect was very low in both set-ups (18εNO2 <1 ‰), which was most likely due to substantial O isotope exchange with ambient water. Moreover, under low-turnover conditions (i.e. in the mineral-only experiments as well as initially in experiments with DB), the observed NO2- isotope systematics suggest, transiently, a small inverse isotope effect (i.e. decreasing NO2- δ15N and δ18O with decreasing concentrations), which was possibly related to transitory surface complexation mechanisms. Site preference (SP) of the 15N isotopes in the linear N2O molecule for both set-ups ranged between 0 ‰ and 14 ‰, which was notably lower than the values previously reported for chemodenitrification. Our results imply that chemodenitrification is dependent on the available reactive surfaces and that the NO2- (rather than the N2O) isotope signatures may be useful for distinguishing between chemodenitrification catalysed by minerals, chemodenitrification catalysed by dead microbial biomass, and possibly true enzymatic NDFeO.

Nitrous oxide emission during nitrification and denitrification in a full-scale night soil treatment plant

1996 ◽  
Vol 34 (1-2) ◽  
pp. 277-284 ◽  
Author(s):  
Hiroki Itokawa ◽  
Keisuke Hanaki ◽  
Tomonori Matsuo
Keyword(s):  
Nitrous Oxide ◽  
Treatment Plant ◽  
Full Scale ◽  
Soil Treatment ◽  
Aeration Tank ◽  
N2o Emissions ◽  
N2o Production ◽  
Mixed Liquor ◽  
Significant Difference ◽  
Nitrification And Denitrification

Nitrous oxide (N2O) emissions from nitrification-denitrification processes in a full-scale night soil treatment plant were measured, and patterns and control of the N2O production were investigated. Estimated N2O emissions ranged from 4.4 to 1,190 gN/(m3 of influent), corresponding to a conversion ratio of influent nitrogen to N2O-N of 0.24-55%. N2O was produced in the intermittent aeration tank (IAT) where nitrification and denitrification were carried out alternately. The produced N2O was either stripped out to the off-gas or remained in the effluent in dissolved form. The former accounted for more than 99.5% of the total emissions. The latter flowed into the following anoxic tank, where 60-98% of N2O was reduced. A significant difference in the extent of N2O supersaturation in mixed liquor of IAT was observed between the cases of high and low N2O emissions. In IAT, N2O tended to be produced discretely either in aerobic or in anoxic phases. It seemed that the completeness of nitrification and denitrification in IAT, indicated from a mass balance between NH4-N and NO3-N and from NO2-N accumulation in mixed liquor of IAT, was one of the important factors affecting the N2O production. This completeness was decided by the time ratio of aerobic and anoxic phases. External addition of methanol to IAT seemed to reduce N2O emissions.

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