scholarly journals Effect of various culture treatments on virulence and aggressiveness expression of Phytophthora infestans

2013 ◽  
Vol 57 (1-2) ◽  
pp. 131-143 ◽  
Author(s):  
Sylwester Sobkowiak ◽  
Ewa Zimoch-Guzowska ◽  
Hanna Zarzycka
Keyword(s):  
Potato Tuber ◽  
Phytophthora Infestans ◽  
Virulence Factors ◽  
Significant Interaction ◽  
Agar Medium ◽  
Potato Tissue ◽  
Inoculum Concentration ◽  
Virulence Spectrum

The effect of experiment date, inoculum concentration and number of passages through potato tissue on virulence spectrum and aggressiveness level of four <i>Phvtophthora infestans</i> isolates was examined in four tests. All these experimental variants significantly influenced the expression of virulence. Isolates chosen for study were characterized initially by complex wirulence and high aggresssiveness. The narro west spectrum of virulence was noted atJune and December in comparison with March and September. The inoculum concentration of 200 sporangia in 1 mm<sup>3</sup> influenced stronger frequency of virulence factors, that concentration of 50 sporangia in 1 mm<sup>3</sup>. The cultures passaged many times showed the wider virulence spectrum than single or double passaged isolates. The double passages of pathogen cultures influenced stronger expression factors of virulence than single one. The significant interaction between experiment dates and number of passages and experiment dales and inoculum concentrations were observed. Higher inoculum concentration and more abundant passages on potato tissues positively affected detection of particular virulence factors, especially v5 and v8. The cultures, both double and many times passaged on potato tuber slices, were significantly more aggressive in comparison with single passaged ones. The isolate MP 425 was the most stable in expression of its virulence and aggressiveness. The <i>P.infestans</i> cultures that lost infectivity on rye agar medium can recover their virulence and aggressiveness expression after double and frequent culturing on potato tissues.

scholarly journals A simple method for diagnostic of Phytophthora infestans (Mont.) de Bary from potato agricultural fields of potato

2015 ◽  
Vol 3 (12) ◽  
pp. 904 ◽  
Author(s):  
Touseef Hussain ◽  
Bir Pal Singh ◽  
Firoz Anwar ◽  
Sonica Tomar
Keyword(s):  
Potato Tuber ◽  
Phytophthora Infestans ◽  
Agar Medium ◽  
Plant Diseases ◽  
Infested Soil ◽  
Continuous Darkness ◽  
High Detection Rate ◽  
Simple Method ◽  
High Detection ◽  
Management Plans

A correct detection and appropriate identification of causal pathogens associated with crop plants or seeds are considered to be the most important issue in designing the proper management plans for plant diseases. This study was designed to detect Phytophthora infestans inoculum from potato grown soil. A high detection rate of P.infestans was obtained from the naturally infested soil of potato fields. Naturally soils were firstly moistened in a plastic pots and then pre-incubated at ±18°C for 3 days, baiting with potato tuber slice for 24, 48, and 72 h. The baits were then thoroughly washed, flooded with 10–15 ml of distilled water in Petri-dishes and incubated under continuous darkness in chamber ±18ºC. Sporangia started to emerge from the margins of potato tuber slice. They were easily observed under the stereomicroscope. Pure culture of the fungus was obtained by isolating from baited tubers on a Rye Agar medium. This is the first report of recovery of P. infestans from naturally infested potato growing soils using susceptible potato tuber (K. Bahar) as bait in India. All isolates were determined to be A2 mating type.

Resistance factors in potato tuber tissue to hyphal growth of Phytophthora infestans

1977 ◽  
Vol 11 (3) ◽  
pp. 267-277 ◽  
Author(s):  
D.D. Clarke ◽  
M.Y.A. Kassim
Keyword(s):  
Potato Tuber ◽  
Phytophthora Infestans ◽  
Hyphal Growth ◽  
Tuber Tissue ◽  
Resistance Factors ◽  
Potato Tuber Tissue

scholarly journals Changes in electrophoretic pattern of soluble proteins of potato tuber in early period of infection by phytophthora infestans.

1982 ◽  
Vol 48 (2) ◽  
pp. 220-223 ◽  
Author(s):  
Norio NISHIMURA ◽  
Kohei TOMIYAMA ◽  
Noriyuki DOKE
Keyword(s):  
Potato Tuber ◽  
Phytophthora Infestans ◽  
Electrophoretic Pattern ◽  
Early Period ◽  
Soluble Proteins

scholarly journals Phytophthora infestans Mating Types on Tomato (Solanum lycopersicum) in Southern Spain

Plant Disease ◽  
2007 ◽  
Vol 91 (1) ◽  
pp. 109-109 ◽  
Author(s):  
J. M. Segura ◽  
M. de Cara ◽  
M. Santos ◽  
J. Tello
Keyword(s):  
Phytophthora Infestans ◽  
Mating Type ◽  
Agar Medium ◽  
The Other ◽  
Southern Spain ◽  
White Mold ◽  
Mating Types ◽  
Tomato Plants ◽  
Cornell University ◽  
Tomato Seeds

During 2004, an unusual spread of Phytophthora infestans on tomato plants in greenhouses located in Almería and Granada provinces, southern Spain, was observed. Infected plants had water-soaked, brown spots on leaves and stems and necrotic areas with white mold on the surface of fruits. Three isolates were obtained by plating diseased tissue on V8 juice agar medium and maintained on rye agar at 18°C. These isolates were analyzed for the mating type. Crosses were carried out using V8 juice agar and rye agar. The two parental isolates US1 (A1) and US8 (A2) were both provided by W. E. Fry, Cornell University, Ithaca, NY. Two of the Spanish isolates were homothallic and the other isolate belonged to the uncommon mating type A1A2. To confirm the occurrence of the two mating types, 43 single-sporangium progeny were produced and analyzed from the A1A2 mating type. Thirty eight isolates were A1, two were A2, one was A1A2 mating type, and two were sterile. Assessment of five single-sporangium progeny from the homothallic type resulted in two A1, two homothallic, and one sterile isolate. A1A2 isolates produced oospores when crossed with either A1 or A2, but not when self-crossed. Previously, the A1A2 mating type has been found in Israel in the field and was obtained from oospores produced on tomato seeds (2,3). Since 2003, mating types of P. infestans isolates recovered from potato (60) and tomato (8) in southern Spain have been characterized. Seventy-five percent of the isolates recovered from potato were A1 and 25% were A2 mating types. Isolates recovered from tomato were 50% A1 and 50% A2 (1). To our knowledge, this is the first report of the occurrence of the A1A2 mating type and homothallic P. infestans isolates on tomato in Spain. References: (1) E. Andujar et al. Congr. Sociedad Española de Fitopatol. 12:244, 2004. (2) E. Rubin and Y. Cohen. Phytoparasitica 32:237, 2004. (3) E. Rubin and Y. Cohen. Plant Dis. 90:741, 2006.

scholarly journals Clade 5 aspartic proteases of Phytophthora infestans are virulence factors implied in RXLR effector cleavage

2019 ◽  
Vol 154 (1) ◽  
pp. 17-29 ◽  
Author(s):  
Charikleia Schoina ◽  
Natalie Verbeek-de Kruif ◽  
Francine Govers ◽  
Klaas Bouwmeester
Keyword(s):  
Phytophthora Infestans ◽  
Virulence Factors ◽  
Aspartic Proteases ◽  
Rxlr Effector

Qualitative and quantitative changes of potato tuber proteome under the influence of signal molecules and infection with Phytophthora infestans

2016 ◽  
Vol 52 (1) ◽  
pp. 71-78 ◽  
Author(s):  
L. G. Yarullina ◽  
R. I. Kasimova ◽  
R. I. Ibragimov ◽  
A. R. Akhatova ◽  
I. A. Umarov ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Phytophthora Infestans ◽  
Signal Molecules ◽  
Qualitative And Quantitative ◽  
Quantitative Changes
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