scholarly journals The structure of the ovary epidermis emitting odorous compounds in Allium karataviense Regel

2014 ◽  
Vol 66 (4) ◽  
pp. 15-24
Author(s):  
Beata Żuraw ◽  
Elżbieta Weryszko-Chmielewska ◽  
Halina Laskowska ◽  
Elżbieta Pogroszewska
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Essential Oils ◽  
Neutral Lipids ◽  
Outer Wall ◽  
Large Cell ◽  
Cell Nuclei ◽  
Histochemical Tests ◽  
Odorous Compounds ◽  
Scanning Electron

Many plants develop in the petal epidermis papillae emitting essential oils. In species of the genus <em>Allium</em>, papillae occur in the ovary epidermis. The aim of this study was to determine the structure of papillae in <em>Allium karataviense </em>Regel and to perform histochemical tests that would allow the components of their secretion to be investigated, mainly the presence of essential oils. Examination was performed using light and scanning electron microscopy. It was found that in conical papillae, with a height of about 83 μm, there were large cell nuclei, a thick outer wall with a layer of striated cuticle, and a varying degree of cell vacuolation, depending on the age of the ovary. Using histochemical reactions, we demonstrated neutral lipids, acid lipids and pectins to occur in the papillae. Neutral lipids are characteristic of essential oils.

scholarly journals Potential of essential oils for the control of brown eye spot in coffee plants

2011 ◽  
Vol 35 (1) ◽  
pp. 115-123 ◽  
Author(s):  
Ricardo Borges Pereira ◽  
Gilvaine Ciavareli Lucas ◽  
Fabiano José Perina ◽  
Mário Lúcio Vilela de Resende ◽  
Eduardo Alves
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Essential Oils ◽  
Mycelial Growth ◽  
Conidia Germination ◽  
Lemon Grass ◽  
Vitro Effect ◽  
Brown Eye Spot ◽  
Scanning Electron

The objectives of this work were to assess the in vitro effect of essential oils extracted from cinnamon, citronella, lemon grass, India clove, tea tree, thyme, neem and eucalyptus on the conidia germination and on mycelial growth of Cercospora coffeicola, and their efficacy to control the brown eye spot in coffee seedlings (cultivars Catucaí 2SL, Catuaí IAC 62 and Mundo Novo 379/19) in a greenhouse, as well as their effects on the initial germination and infection events by scanning electron microscopy. All essential oils promoted the inhibition of conidia germination with increasing concentrations. India clove, cinnamon, neem, thyme and lemon grass oils inhibited the mycelial growth of C. coffeicola. The cinnamon and citronella oils were the most promising for brown eye spot control in all cultivars. In scanning electron microscopy, the cinnamon and citronella oils reduced germination and mycelial development of C. coffeicola in vivo, eight and 16 hours after inoculation, promoting, in some cases, the leakage of the cellular content. Essential oils of cinnamon and citronella reduced the incidence and severity of brown eye spot, in addition to presenting direct toxicity to the pathogen.

Anatomical characterisation of the foliar colleters in Myrtoideae (Myrtaceae)

2012 ◽  
Vol 60 (8) ◽  
pp. 707 ◽  
Author(s):  
Cleber J. da Silva ◽  
Luiz Cláudio de A. Barbosa ◽  
Ana E. Marques ◽  
Maria Cristina Baracat-Pereira ◽  
Antônio L. Pinheiro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Relationships ◽  
External Morphology ◽  
Secretory Structures ◽  
Reproductive Shoot ◽  
Sds Page ◽  
Histochemical Tests ◽  
Five Tribes ◽  
Scanning Electron

Colleters are secretory structures that occur in vegetative or reproductive shoot apices of many botanical families. However, in the order Myrtales, reports of colleters have considered only external morphology. We therefore evaluated apical meristems of 52 species belonging to 17 genera from seven tribes of subfamily Myrtoideae (Myrtaceae), so as to analyse the incidence and morphological types of colleters. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on fresh and methacrylate-embedded material. Proteins of the colleter secretions were analysed by SDS-PAGE. We have classified and described the following three new colleter types: petaloid, conic and euryform. None of the species contained all three colleter types. The petaloid colleters were present in three tribes (Syzygieae, Melaleuceae and Lophostemoneae). The conic colleters were observed in three tribes (Leptospermeae, Myrteae and Melaleuceae) and the euryform type occurred in five tribes (Leptospermeae, Syncarpieae, Myrteae, Syzygieae and Melaleuceae). In the tribe Eucalypteae, we found no evidence of colleters. The presence of mucilaginous secretion that defines colleters was confirmed by histochemical tests, and no proteins were found in the secretion. The colleters in Myrtoideae may help clarify the phylogenetic relationships of the Myrtaceae family.

scholarly journals Contribution to the identification of the species Myrcia hatschbachii D. Legrand (Myrtaceae): anatomical and histochemical analyses

2021 ◽  
Vol 43 ◽  
pp. e75
Author(s):  
Larissa Junqueira Gatto ◽  
Vanessa Barbosa Bobek ◽  
Jane Manfron Budel ◽  
Natasha Tiemi Fabri ◽  
Josiane De Fatima Gaspari Dias ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phenolic Compounds ◽  
Vascular Bundle ◽  
Folk Medicine ◽  
Spongy Parenchyma ◽  
Anatomical Features ◽  
Arc Shape ◽  
Histochemical Tests ◽  
Scanning Electron

The genus Myrcia is used in folk medicine to treat diabetes. The plants used in folk medicine require morphological and anatomical references to attest to its authenticity. This is the first report of the microscopic study of Myrcia hatschbachii. In this sense, the aim of the present study was to describe the anatomical characteristics, in order to contribute to the species' identification. For anatomical and surface analyses free-hand sections were prepared and observed using optical microscopy, simultaneously some materials were processed and observed under scanning electron microscopy. In addition, histochemical tests were performed. The anatomical features described here correspond with previously reported features found in other species of the genus Myrcia. Furthermore, the following anatomical markers were observed in Myrcia hatschbachii: druses in the spongy parenchyma, concave-convex midrib, heart-shaped petiole, oval-shaped stem, C-shaped vascular bundle in the midrib and open arc shape with invaginated ends in the petiole; phenolic compounds in the phloem and lignified cells in the pith. The results of anatomical and histochemical analyses provide micromorphological and microchemical features that can help in the taxonomy and proper identification of the species.

scholarly journals New registers of colleters in species of Myrtaceae from Brazilian Cerrado

Rodriguésia ◽  
2019 ◽  
Vol 70 ◽  
Author(s):  
Cleber José da Silva ◽  
João Paulo Oliveira Ribeiro ◽  
Renata Maria Strozi Alves Meira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Fleshy Fruit ◽  
Brazilian Cerrado ◽  
Secretory Structures ◽  
Histochemical Tests ◽  
Potential Use ◽  
Scanning Electron

Abstract Colleters are secretory structures that produce a sticky substance, consisting of a mixture of mucilage, proteins, terpenes, pectic substances and even alkaloids, which lubricates and protects the shoot apical meristem. Several colleter types have been described and have taxonomic value in many botanical families. In Myrtaceae, the colleters description is recent and presents three new morphological types (conic, euryform and petaloid) that differ those already described for other eudicots. In this work, we report the colleters morphological types in six species of three genera belonging to the Myrteae tribe of Myrtoideae from the Brazilian Cerrado. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on the fresh and methacrylate-embedded material. The conic and euryform colleters from Myrtoideae species of the Cerrado did not differ either morphologically nor as to the secretion nature from those described for Myrtoideae species from others biomes, which may indicate their potential use for taxonomic purposes. Considering the hypothesis that the multiple fleshy-fruit lineages have evolved independently in Myrteae tribe, our results indicate the relevance of additional studies in order to recognize the pattern of distribution of colleters in Myrtaceae.

Optimization of the Antimicrobial Effects of Surfactin against Bacillus cereus Spores

2020 ◽  
Vol 83 (11) ◽  
pp. 1983-1988
Author(s):  
XIANQING HUANG ◽  
LIANJUN SONG ◽  
MINGWU QIAO ◽  
PINGAN ZHANG ◽  
QIUYAN ZHAO
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bacillus Cereus ◽  
Control Sample ◽  
Outer Wall ◽  
Experimental Results ◽  
Optimal Conditions ◽  
Design Expert ◽  
Temperature Time ◽  
Scanning Electron

ABSTRACT The purpose of this study was to establish a three-variable bactericidal model of temperature, time, and concentration to determine the optimal conditions for Bacillus cereus spore inactivation by surfactin. To obtain the binary regression equation of the inactivated spore model, a total of 17 simulations were performed using response surface methodology. The experimental results showed that the three factors each had a discernible but nonequal impact on the inactivation response value. Multiple regression analysis of experimental results using Design-Expert software yielded the following equation: Y = 1.47 + 0.39ξ1(temperature) + 0.38ξ2(time) + 0.39ξ3(concentration) − 0.20ξ1ξ2 + 0.22ξ1ξ2 − 0.12ξ2ξ3 − 0.23ξ12 − 0.11ξ22 − 0.40ξ32. Optimal inactivation of spores was achieved by treatment with surfactin at a concentration of 4 mg/mL for 40 h at 53°C, with the response value reaching 1.8. The spores were treated with surfactin under these conditions; the microstructural changes of spores were observed by use of scanning electron microscopy. We found that the structures of the outer wall of the spores were damaged, whereas the spores in the control sample showed no visible damage. HIGHLIGHTS

Scanning electron microscopy and histochemistry of embryonic envelopes of the porcupine tapeworm, Monoecocestus americanus (Cyclophyllidea: Anoplocephalidae)

1985 ◽  
Vol 63 (5) ◽  
pp. 1194-1198 ◽  
Author(s):  
David Bruce Conn
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
North American ◽  
Basic Structure ◽  
Gravid Uterus ◽  
Uterine Lumen ◽  
Acid Mucopolysaccharides ◽  
Histochemical Tests ◽  
Scanning Electron

Eggs of Monoecocestus americanus (Stiles 1895), a tapeworm of North American porcupines, were studied with regard to the basic structure of embryonic envelopes. Observations were made by light microscopy of whole eggs and scanning electron microscopy of eggs within cryofractured gravid proglottids. Concomitant histochemical tests were made on eggs and gravid proglottids embedded and sectioned in methacrylate plastic. The gravid uterus had numerous diverticula, each containing several eggs. The uterine lumen was filled with a material positive for nonsulfated acid mucopolysaccharides. The material adhered to the eggs, giving them their characteristic spinose surface when teased from the proglottid. Each egg included an oncosphere larva surrounded by an oncospheral membrane, pyriform embryophore, inner and outer envelopes, subshell membrane, and embryonic capsule. Overall, the structural and histochemical characteristics of these layers were similar to those described for other cyclophyllideans, with only minor variations.

scholarly journals Morphology, distribution, and histochemistry of trichomes of Thymus lykae Degen & Jav. (Lamiaceae)

2008 ◽  
Vol 60 (4) ◽  
pp. 667-672 ◽  
Author(s):  
Marija Marin ◽  
Snezana Budimir ◽  
Dusica Janosevic ◽  
P.D. Marin ◽  
Sonja Duletic-Lausevic ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Laser Scanning ◽  
Glandular Trichomes ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Capitate Trichomes ◽  
Histochemical Tests ◽  
Scanning Electron

Micromorphology, distribution, and histochemistry of the trichomes of Thymus lykae were studied using scanning electron microscopy (SEM)and confocal laser scanning electron microscopy (CLSM). The leaves, stem, and calyx bear numerous glandular and non-glandular trichomes. Two types of glandular trichomes are identified - peltate and capitate. Results of histochemical tests showed positive reactions to polysaccharides, proteins, and lipids. Yellow autofluorescence of secreted material was noticed in peltate and capitate trichomes. Strong reddish-yellow autofluorescence of the lipophilic and hydrophilic secreted material was observed with CLSM.

scholarly journals The micromorphological, histochemical and confocal analysis of satureja subspicata Bartl. ex vis. glandular trichomes

2010 ◽  
Vol 62 (4) ◽  
pp. 1143-1149 ◽  
Author(s):  
Marija Marin ◽  
N. Jasnic ◽  
D. Lakusic ◽  
Sonja Duletic-Lausevic ◽  
Lia Ascensao
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Positive Reaction ◽  
Laser Scanning ◽  
Glandular Trichomes ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Histochemical Tests ◽  
Scanning Electron

Micromorphology, histochemical and confocal analyses of the trichomes of Satureja subspicata (Bartl. ex Vis.) were carried out using light microscopy, confocal laser scanning electron microscopy (CLSM), and scanning electron microscopy. Non-glandular unbranched and two types of glandular trichomes - peltate and capitate - are described. The results of histochemical tests showed a positive reaction to phenolics, tannins, lipids, acid lipids, pectins and polysaccharides in both types of glandular trichomes. A strong red autofluorescence of the lipophilic and hydrophilic secreted material in glandular trichomes was observed with CLSM.

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