scholarly journals New registers of colleters in species of Myrtaceae from Brazilian Cerrado

Rodriguésia ◽  
2019 ◽  
Vol 70 ◽  
Author(s):  
Cleber José da Silva ◽  
João Paulo Oliveira Ribeiro ◽  
Renata Maria Strozi Alves Meira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Fleshy Fruit ◽  
Brazilian Cerrado ◽  
Secretory Structures ◽  
Histochemical Tests ◽  
Potential Use ◽  
Scanning Electron

Abstract Colleters are secretory structures that produce a sticky substance, consisting of a mixture of mucilage, proteins, terpenes, pectic substances and even alkaloids, which lubricates and protects the shoot apical meristem. Several colleter types have been described and have taxonomic value in many botanical families. In Myrtaceae, the colleters description is recent and presents three new morphological types (conic, euryform and petaloid) that differ those already described for other eudicots. In this work, we report the colleters morphological types in six species of three genera belonging to the Myrteae tribe of Myrtoideae from the Brazilian Cerrado. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on the fresh and methacrylate-embedded material. The conic and euryform colleters from Myrtoideae species of the Cerrado did not differ either morphologically nor as to the secretion nature from those described for Myrtoideae species from others biomes, which may indicate their potential use for taxonomic purposes. Considering the hypothesis that the multiple fleshy-fruit lineages have evolved independently in Myrteae tribe, our results indicate the relevance of additional studies in order to recognize the pattern of distribution of colleters in Myrtaceae.

Anatomical characterisation of the foliar colleters in Myrtoideae (Myrtaceae)

2012 ◽  
Vol 60 (8) ◽  
pp. 707 ◽  
Author(s):  
Cleber J. da Silva ◽  
Luiz Cláudio de A. Barbosa ◽  
Ana E. Marques ◽  
Maria Cristina Baracat-Pereira ◽  
Antônio L. Pinheiro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Relationships ◽  
External Morphology ◽  
Secretory Structures ◽  
Reproductive Shoot ◽  
Sds Page ◽  
Histochemical Tests ◽  
Five Tribes ◽  
Scanning Electron

Colleters are secretory structures that occur in vegetative or reproductive shoot apices of many botanical families. However, in the order Myrtales, reports of colleters have considered only external morphology. We therefore evaluated apical meristems of 52 species belonging to 17 genera from seven tribes of subfamily Myrtoideae (Myrtaceae), so as to analyse the incidence and morphological types of colleters. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on fresh and methacrylate-embedded material. Proteins of the colleter secretions were analysed by SDS-PAGE. We have classified and described the following three new colleter types: petaloid, conic and euryform. None of the species contained all three colleter types. The petaloid colleters were present in three tribes (Syzygieae, Melaleuceae and Lophostemoneae). The conic colleters were observed in three tribes (Leptospermeae, Myrteae and Melaleuceae) and the euryform type occurred in five tribes (Leptospermeae, Syncarpieae, Myrteae, Syzygieae and Melaleuceae). In the tribe Eucalypteae, we found no evidence of colleters. The presence of mucilaginous secretion that defines colleters was confirmed by histochemical tests, and no proteins were found in the secretion. The colleters in Myrtoideae may help clarify the phylogenetic relationships of the Myrtaceae family.

Echinostoma revolution (Froelich, 1802) Looss, 1899 and Isthmiophora melis (Schrank, 1788) Lühe, 1909 (Echinostomatinae, Digenea): Scanning electron microscopy of the tegumental surfaces

1984 ◽  
Vol 58 (3) ◽  
pp. 187-195 ◽  
Author(s):  
Lesley R. Smales ◽  
Harvey D. Blankespoor
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Topography ◽  
Body Surface ◽  
Secretory Granules ◽  
The Body ◽  
Taxonomic Character ◽  
Secretory Structures ◽  
Unusual Structure ◽  
Scanning Electron

ABSTRACTA detailed study of the surface topography of the echinostomes Echinostoma revolution and Isthmiophora melis has been carried out using SEM. The tegument of the body surface has a cobblestone-like appearance with interspersed pits. The presence of possible secretory granules is noted. Observations of the anterior end reveal that the collar spines are retractable. An unusual structure is described from the ventral gap of the spiny collar of E. revolutum. The origins of this structure and the possible functions of the spiny collar are discussed. The shape and distribution of body spines is described. Differences in cirrus morphology for the two species are described. The taxonomic position of, I. melis and the significance of cirrus morphology as a useful taxonomic character are discussed. Four types of presumed sensory or secretory structures occur—ciliated, domed, button and bilobed papillae. Their distribution is described and possible functions discussed.

scholarly journals Contribution to the identification of the species Myrcia hatschbachii D. Legrand (Myrtaceae): anatomical and histochemical analyses

2021 ◽  
Vol 43 ◽  
pp. e75
Author(s):  
Larissa Junqueira Gatto ◽  
Vanessa Barbosa Bobek ◽  
Jane Manfron Budel ◽  
Natasha Tiemi Fabri ◽  
Josiane De Fatima Gaspari Dias ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phenolic Compounds ◽  
Vascular Bundle ◽  
Folk Medicine ◽  
Spongy Parenchyma ◽  
Anatomical Features ◽  
Arc Shape ◽  
Histochemical Tests ◽  
Scanning Electron

The genus Myrcia is used in folk medicine to treat diabetes. The plants used in folk medicine require morphological and anatomical references to attest to its authenticity. This is the first report of the microscopic study of Myrcia hatschbachii. In this sense, the aim of the present study was to describe the anatomical characteristics, in order to contribute to the species' identification. For anatomical and surface analyses free-hand sections were prepared and observed using optical microscopy, simultaneously some materials were processed and observed under scanning electron microscopy. In addition, histochemical tests were performed. The anatomical features described here correspond with previously reported features found in other species of the genus Myrcia. Furthermore, the following anatomical markers were observed in Myrcia hatschbachii: druses in the spongy parenchyma, concave-convex midrib, heart-shaped petiole, oval-shaped stem, C-shaped vascular bundle in the midrib and open arc shape with invaginated ends in the petiole; phenolic compounds in the phloem and lignified cells in the pith. The results of anatomical and histochemical analyses provide micromorphological and microchemical features that can help in the taxonomy and proper identification of the species.

Scanning electron microscopy and histochemistry of embryonic envelopes of the porcupine tapeworm, Monoecocestus americanus (Cyclophyllidea: Anoplocephalidae)

1985 ◽  
Vol 63 (5) ◽  
pp. 1194-1198 ◽  
Author(s):  
David Bruce Conn
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
North American ◽  
Basic Structure ◽  
Gravid Uterus ◽  
Uterine Lumen ◽  
Acid Mucopolysaccharides ◽  
Histochemical Tests ◽  
Scanning Electron

Eggs of Monoecocestus americanus (Stiles 1895), a tapeworm of North American porcupines, were studied with regard to the basic structure of embryonic envelopes. Observations were made by light microscopy of whole eggs and scanning electron microscopy of eggs within cryofractured gravid proglottids. Concomitant histochemical tests were made on eggs and gravid proglottids embedded and sectioned in methacrylate plastic. The gravid uterus had numerous diverticula, each containing several eggs. The uterine lumen was filled with a material positive for nonsulfated acid mucopolysaccharides. The material adhered to the eggs, giving them their characteristic spinose surface when teased from the proglottid. Each egg included an oncosphere larva surrounded by an oncospheral membrane, pyriform embryophore, inner and outer envelopes, subshell membrane, and embryonic capsule. Overall, the structural and histochemical characteristics of these layers were similar to those described for other cyclophyllideans, with only minor variations.

scholarly journals Enhancement of Growth and Flowering of Triteleia laxa by Ethylene

1990 ◽  
Vol 115 (3) ◽  
pp. 482-486 ◽  
Author(s):  
Susan S. Han ◽  
Abraham H. Halevy ◽  
Roy M. Sachs ◽  
Michael S. Reid
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Growth Rate ◽  
Respiration Rate ◽  
Apical Meristem ◽  
Day Treatment ◽  
Fresh Weight ◽  
Final Size ◽  
Meristem Size ◽  
Scanning Electron

Exposure of dormant corms of Triteleia laxa `Queen Fabiola' to 20 ppm C2H4 for 7 days promoted flowering of small corms and resulted in increased apical meristem size, early sprouting, early flowering, more flowers per Inflorescence, and increased fresh weight of daughter corms and cormels. The respiration rate of the C&treated corms increased to four to five times that of the controls during the 7-day treatment, declined markedly after termination of the C2H4 treatment, but remained higher than that of the controls. The C2H4 effects were associated with increased growth rate and consequently a greater final size of the apical meristem (determined by scanning electron microscopy). Leaves produced by C2H4-treated corms were wider, longer, and weighed more than those of the controls.

scholarly journals Morphology, distribution, and histochemistry of trichomes of Thymus lykae Degen & Jav. (Lamiaceae)

2008 ◽  
Vol 60 (4) ◽  
pp. 667-672 ◽  
Author(s):  
Marija Marin ◽  
Snezana Budimir ◽  
Dusica Janosevic ◽  
P.D. Marin ◽  
Sonja Duletic-Lausevic ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Laser Scanning ◽  
Glandular Trichomes ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Capitate Trichomes ◽  
Histochemical Tests ◽  
Scanning Electron

Micromorphology, distribution, and histochemistry of the trichomes of Thymus lykae were studied using scanning electron microscopy (SEM)and confocal laser scanning electron microscopy (CLSM). The leaves, stem, and calyx bear numerous glandular and non-glandular trichomes. Two types of glandular trichomes are identified - peltate and capitate. Results of histochemical tests showed positive reactions to polysaccharides, proteins, and lipids. Yellow autofluorescence of secreted material was noticed in peltate and capitate trichomes. Strong reddish-yellow autofluorescence of the lipophilic and hydrophilic secreted material was observed with CLSM.

scholarly journals The micromorphological, histochemical and confocal analysis of satureja subspicata Bartl. ex vis. glandular trichomes

2010 ◽  
Vol 62 (4) ◽  
pp. 1143-1149 ◽  
Author(s):  
Marija Marin ◽  
N. Jasnic ◽  
D. Lakusic ◽  
Sonja Duletic-Lausevic ◽  
Lia Ascensao
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Positive Reaction ◽  
Laser Scanning ◽  
Glandular Trichomes ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Histochemical Tests ◽  
Scanning Electron

Micromorphology, histochemical and confocal analyses of the trichomes of Satureja subspicata (Bartl. ex Vis.) were carried out using light microscopy, confocal laser scanning electron microscopy (CLSM), and scanning electron microscopy. Non-glandular unbranched and two types of glandular trichomes - peltate and capitate - are described. The results of histochemical tests showed a positive reaction to phenolics, tannins, lipids, acid lipids, pectins and polysaccharides in both types of glandular trichomes. A strong red autofluorescence of the lipophilic and hydrophilic secreted material in glandular trichomes was observed with CLSM.

Early stages of initiation of two types of leaves in Thuja occidentalis (eastern white cedar)

2004 ◽  
Vol 82 (5) ◽  
pp. 598-606 ◽  
Author(s):  
Christian Lacroix ◽  
Denis Barabé ◽  
Bernard Jeune
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Apical Meristem ◽  
Leaf Primordia ◽  
Leaf Primordium ◽  
Thuja Occidentalis ◽  
White Cedar ◽  
Early Stages ◽  
Eastern White Cedar ◽  
Scanning Electron

The developmental morphology of shoots of Thuja occidentalis L. (eastern white cedar) was investigated using scanning electron microscopy to determine the pattern of initiation of two types of leaves characteristic of higher (third and above) order branches. The shoots of eastern white cedar are bilateral in symmetry and bear leaves in an orthogonal decussate phyllotactic pattern. The shoot system is further characterized by the presence of two alternating and morphologically different pairs of leaves that constitute the basic repeating pattern of the shoot apical meristem (SAM). At maturity the dimorphism between leaf types is marked. Leaves in one plane are wide and flat in comparison with narrower and cup-shaped leaves growing in a plane perpendicular to the other leaf type. The early stages of development of each of the two types of leaves were compared using scanning electron microscopy. During the earliest visible stages of initiation (primordial crest), cup-shaped and flat leaves are very similar in morphology. As individual leaf primordia become more easily delimited as structures by the presence of a furrow between the SAM and the leaf, they differ in terms of width. As they develop further and begin to cover the SAM, the two leaf types are distinguishable morphologically (flat vs. cup shaped). Quantitative parameters such as diameter of the SAM, angle of insertion of individual leaves, and size of leaf primordia (in both a tangential and perpendicular plane) were measured on three categories of leaves: stage 1, earliest visible stage of initiation; stage 2, delineation of leaf primordium from SAM by furrowing; stage 3, leaf primordium partially covering SAM. These measurements corroborate our morphological observations, which show that during early stages of development, flat and cup-shaped leaves are morphologically similar and they diverge in their pattern of development postinitiation, especially as far as leaf width and thickness are concerned. Our results also suggest that the size and shape of the apex goes through a "repeating" cycle and is related to the type of primordium that will be initiated.Key words: Thuja occidentalis, eastern white cedar, leaf development, shoot apical meristem, phyllotaxy, leaf dimorphism.

Complex tendril branching in two species of Parthenocissus: implications for the vitaceous shoot architecture

2003 ◽  
Vol 81 (6) ◽  
pp. 587-597 ◽  
Author(s):  
Trevor Wilson ◽  
Usher Posluszny
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Apical Meristem ◽  
Lateral Growth ◽  
Developmental Morphology ◽  
Main Shoot ◽  
Lateral Buds ◽  
Shoot Architecture ◽  
Parthenocissus Quinquefolia ◽  
Scanning Electron

This study compares the developmental morphology of Parthenocissus quinquefolia Planch. (L.) (Virginia creeper) with that of Parthenocissus tricuspidata (Sieb. & Zucc.) Planch. (Boston ivy) to better understand how the tendril is related to the main shoot architecture of the Vitaceae. Tendril development is looked at from inception to maturity using scanning electron microscopy and epi-illumination microscopy. A tendril primordium is initiated low on the periphery of a shoot apical meristem; this pattern of development is reminiscent of typical monopodial shoot development. A tendril "shoot" in turn produces several bract-opposed branches and resembles the main shoot in its early development. However, a tendril develops through lateral growth, and because it lacks lateral buds, its architecture appears more sympodial than the main shoot. As more tendril branches are produced, the tendril "shoot" pattern of development gradually changes to a pattern of development reported in other vitaceous tendrils. This shift in development suggests how other vitaceous tendrils may be related to the shoot. Because tendril development in Parthenocissus can be demonstrated to be sympodial, it lends evidence to the idea that the unique monopodial-like system in members of the Vitaceae may be derived. The main shoot and tendril development are more similar than has previously been shown.Key words: Vitaceae, Parthenocissus, monopodial, sympodial, tendril, development.

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