scholarly journals Contribution to the identification of the species Myrcia hatschbachii D. Legrand (Myrtaceae): anatomical and histochemical analyses

2021 ◽  
Vol 43 ◽  
pp. e75
Author(s):  
Larissa Junqueira Gatto ◽  
Vanessa Barbosa Bobek ◽  
Jane Manfron Budel ◽  
Natasha Tiemi Fabri ◽  
Josiane De Fatima Gaspari Dias ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phenolic Compounds ◽  
Vascular Bundle ◽  
Folk Medicine ◽  
Spongy Parenchyma ◽  
Anatomical Features ◽  
Arc Shape ◽  
Histochemical Tests ◽  
Scanning Electron

The genus Myrcia is used in folk medicine to treat diabetes. The plants used in folk medicine require morphological and anatomical references to attest to its authenticity. This is the first report of the microscopic study of Myrcia hatschbachii. In this sense, the aim of the present study was to describe the anatomical characteristics, in order to contribute to the species' identification. For anatomical and surface analyses free-hand sections were prepared and observed using optical microscopy, simultaneously some materials were processed and observed under scanning electron microscopy. In addition, histochemical tests were performed. The anatomical features described here correspond with previously reported features found in other species of the genus Myrcia. Furthermore, the following anatomical markers were observed in Myrcia hatschbachii: druses in the spongy parenchyma, concave-convex midrib, heart-shaped petiole, oval-shaped stem, C-shaped vascular bundle in the midrib and open arc shape with invaginated ends in the petiole; phenolic compounds in the phloem and lignified cells in the pith. The results of anatomical and histochemical analyses provide micromorphological and microchemical features that can help in the taxonomy and proper identification of the species.

scholarly journals Structural features of flower trichomes in drug eyebright (Euphrasia stricta D. Wolff EX J. F. Lehm.)

2014 ◽  
Vol 66 (4) ◽  
pp. 35-44 ◽  
Author(s):  
Weronika Haratym ◽  
Elżbieta Weryszko-Chmielewska
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Glandular Trichomes ◽  
Folk Medicine ◽  
Structural Features ◽  
Raw Material ◽  
Wall Thickening ◽  
Anatomical Features ◽  
Ophthalmic Diseases ◽  
Scanning Electron

<em>Euphrasia stricta </em>D. Wolff ex J. F. Lehm. (Orobanchaceae) is a representative of plants that are widely used in folk medicine, phytomedicine, and homeopathy. The medicinal raw material derived from the drug eyebright is applied primarily in treatment of ophthalmic diseases. The investigations of trichomes in drug eyebright (<em>Euphrasia stricta </em>D. Wolff ex J. F. Lehm) were conducted in 2010–2011. Using light microscopy and scanning electron microscopy, their location and morphological and anatomical features were identified. Three types of non-glandular trichomes were found: short unicellular, long 1–2 celled, and long 2-celled with wall ornamentation. Additionally, 7 types of glandular trichomes were found; these included: unicellular clavate, 2–3-celled clavate, capitate with a unicellular head and a 3-cel- led stalk, capitate with a unicellular head and a 2-celled stalk, capitate with a 2-celled head, conical papillae, and ribbon-like trichomes with wall thickening.

Anatomical characterisation of the foliar colleters in Myrtoideae (Myrtaceae)

2012 ◽  
Vol 60 (8) ◽  
pp. 707 ◽  
Author(s):  
Cleber J. da Silva ◽  
Luiz Cláudio de A. Barbosa ◽  
Ana E. Marques ◽  
Maria Cristina Baracat-Pereira ◽  
Antônio L. Pinheiro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Relationships ◽  
External Morphology ◽  
Secretory Structures ◽  
Reproductive Shoot ◽  
Sds Page ◽  
Histochemical Tests ◽  
Five Tribes ◽  
Scanning Electron

Colleters are secretory structures that occur in vegetative or reproductive shoot apices of many botanical families. However, in the order Myrtales, reports of colleters have considered only external morphology. We therefore evaluated apical meristems of 52 species belonging to 17 genera from seven tribes of subfamily Myrtoideae (Myrtaceae), so as to analyse the incidence and morphological types of colleters. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on fresh and methacrylate-embedded material. Proteins of the colleter secretions were analysed by SDS-PAGE. We have classified and described the following three new colleter types: petaloid, conic and euryform. None of the species contained all three colleter types. The petaloid colleters were present in three tribes (Syzygieae, Melaleuceae and Lophostemoneae). The conic colleters were observed in three tribes (Leptospermeae, Myrteae and Melaleuceae) and the euryform type occurred in five tribes (Leptospermeae, Syncarpieae, Myrteae, Syzygieae and Melaleuceae). In the tribe Eucalypteae, we found no evidence of colleters. The presence of mucilaginous secretion that defines colleters was confirmed by histochemical tests, and no proteins were found in the secretion. The colleters in Myrtoideae may help clarify the phylogenetic relationships of the Myrtaceae family.

scholarly journals New registers of colleters in species of Myrtaceae from Brazilian Cerrado

Rodriguésia ◽  
2019 ◽  
Vol 70 ◽  
Author(s):  
Cleber José da Silva ◽  
João Paulo Oliveira Ribeiro ◽  
Renata Maria Strozi Alves Meira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Fleshy Fruit ◽  
Brazilian Cerrado ◽  
Secretory Structures ◽  
Histochemical Tests ◽  
Potential Use ◽  
Scanning Electron

Abstract Colleters are secretory structures that produce a sticky substance, consisting of a mixture of mucilage, proteins, terpenes, pectic substances and even alkaloids, which lubricates and protects the shoot apical meristem. Several colleter types have been described and have taxonomic value in many botanical families. In Myrtaceae, the colleters description is recent and presents three new morphological types (conic, euryform and petaloid) that differ those already described for other eudicots. In this work, we report the colleters morphological types in six species of three genera belonging to the Myrteae tribe of Myrtoideae from the Brazilian Cerrado. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on the fresh and methacrylate-embedded material. The conic and euryform colleters from Myrtoideae species of the Cerrado did not differ either morphologically nor as to the secretion nature from those described for Myrtoideae species from others biomes, which may indicate their potential use for taxonomic purposes. Considering the hypothesis that the multiple fleshy-fruit lineages have evolved independently in Myrteae tribe, our results indicate the relevance of additional studies in order to recognize the pattern of distribution of colleters in Myrtaceae.

Scanning electron microscopy and histochemistry of embryonic envelopes of the porcupine tapeworm, Monoecocestus americanus (Cyclophyllidea: Anoplocephalidae)

1985 ◽  
Vol 63 (5) ◽  
pp. 1194-1198 ◽  
Author(s):  
David Bruce Conn
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
North American ◽  
Basic Structure ◽  
Gravid Uterus ◽  
Uterine Lumen ◽  
Acid Mucopolysaccharides ◽  
Histochemical Tests ◽  
Scanning Electron

Eggs of Monoecocestus americanus (Stiles 1895), a tapeworm of North American porcupines, were studied with regard to the basic structure of embryonic envelopes. Observations were made by light microscopy of whole eggs and scanning electron microscopy of eggs within cryofractured gravid proglottids. Concomitant histochemical tests were made on eggs and gravid proglottids embedded and sectioned in methacrylate plastic. The gravid uterus had numerous diverticula, each containing several eggs. The uterine lumen was filled with a material positive for nonsulfated acid mucopolysaccharides. The material adhered to the eggs, giving them their characteristic spinose surface when teased from the proglottid. Each egg included an oncosphere larva surrounded by an oncospheral membrane, pyriform embryophore, inner and outer envelopes, subshell membrane, and embryonic capsule. Overall, the structural and histochemical characteristics of these layers were similar to those described for other cyclophyllideans, with only minor variations.

scholarly journals Comparative structure of the osmophores in the flowers of Stanhopea graveolens Lindley and Cycnoches chlorochilon Klotzsch (Orchidaceae)

2012 ◽  
Vol 64 (2) ◽  
pp. 15-24 ◽  
Author(s):  
Mirosława Chwil
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Apical Part ◽  
Ornamental Plant ◽  
Abaxial Surface ◽  
Glandular Hairs ◽  
Anatomical Features ◽  
Cuticular Ornamentation ◽  
Comparative Structure ◽  
Scanning Electron

The genus <i>Tradescantia</i> comprises about 70 species. In Poland <i>Tradescantia x andersoniana</i> is basically grown as an ornamental plant that is recommended for borders and to be planted around garden ponds. The present study investigated flowering as well as the micromorphological and anatomical features of some floral elements of <i>Tradescantia x andersoniana</i> W. Ludw. Rohweder 'Karin'. The macro- and micromorphology of the flowers was examined using stereoscopic, light, and scanning electron microscopy (SEM). Spiderwort produces flowers with a diameter of 4.6-5 cm, which open early in the morning and close at noon. Non-glandular and glandular hairs grow on the abaxial surface of the calyx and on the apical part of the ovary. The glandular hairs develop a several-celled stalk and a unicellular spherical or elongated head. The staminal filaments produce chain-shaped trichomes. Striate cuticular ornamentation is found on their surface and on the epidermis covering the perianth. The striae on the cells of the stamen hairs run to the two poles of the cell. The pistil develops a wet stigma with unfused unicellular papillae. The cuticle on their surface is smooth, whereas on the style near the stigma it forms dense folds.

scholarly journals Comparison of synandrium structure and development in three species from the Myristicaceae

Botany ◽  
2017 ◽  
Vol 95 (1) ◽  
pp. 89-99 ◽  
Author(s):  
Gui-Fang Yang ◽  
Feng-Xia Xu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Vascular Bundle ◽  
Developmental Stages ◽  
Floral Meristem ◽  
Stamen Primordia ◽  
Floral Apex ◽  
Morphology And Structure ◽  
Myristica Fragrans Houtt ◽  
Scanning Electron

Species of Myristicaceae have diverse morphology and structure of their synandria, making them an interesting group for androecium evolution research. To clarify the morphology, structure, and origin of the synandrium, scanning electron microscopy and histology were performed on staminate flowers of Horsfieldia pandurifolia H.H.Hu, H. tetratepala C.Y.Wu & W.T.Wang, and Myristica fragrans Houtt. at different developmental stages. A whorl of stamen primordia was rapidly initiated around the margin of the floral apex in groups of two in H. pandurifolia and groups of three in M. fragrans. Each stamen primordium grew longitudinally, producing a pair of anther lobes and four microsporangia, accompanying one vascular bundle in H. pandurifolia and M. fragrans. In H. tetratepala, three stamen primordia groups were formed, and each group with several anthers was supported by one vascular bundle, indicating a secondary increase of stamen. Three types of synandrium origin were observed: in M. fragrans, the central sterile column tissues originated from the elongate receptacle; in H. pandurifolia and H. tetratepala they were derived from a combination of floral meristem and fused connectives, and a combination of receptacular tissue and stamen groups. The diverse origins of the central sterile column suggest that the synandrium develops differently and independently in different genera and species of Myristicaceae.

scholarly journals Comparative morphology and anatomy of seeds of some Aethionema W.T. Aiton (Brassicaceae) taxa from Turkey

2019 ◽  
Vol 26 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Mehmet Cengiz Karaismailoğlu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Principal Component Analysis ◽  
Principal Component ◽  
Comparative Morphology ◽  
Seed Morphology ◽  
Anatomical Features ◽  
Major Significance ◽  
Surface Patterns ◽  
Scanning Electron

Seed morphology and anatomy are taxonomically significant in Brassicaceae. The seed structures of Aethionema from Turkey, which include 12 taxa (Aethionema syriacum, A. froedinii, A. arabicum, A. eunomioides, A. fimbriatum, A. speciosum subsp. speciosum, A. speciosum subsp. compactum, A. saxatile, A. oppositifolium, A. iberideum, A. armenum, A. grandiflorum) were studied for knowing seed morphological and anatomical features with one-way analysis of variance, cluster analysis and principal component analysis. Seed size, shape and color were examined with stereomicroscopy.The surface patterns of seed were observed using Scanning Electron Microscopy (SEM). In addition, structure, and thicknesses of testa and endosperm were investigated anatomically. Thickness of testa and endosperm were of major significance to illustrate interspecific relations among the examined taxa.

scholarly journals Anatomy and trichome micromorphology of Stachys scardica (Griseb.) Hayek (Lamiaceae)

2014 ◽  
Vol 66 (3) ◽  
pp. 1217-1226 ◽  
Author(s):  
Slavica Grujic ◽  
Sonja Duletic-Lausevic ◽  
Ana Dzamic ◽  
P.D. Marin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Anatomical Structure ◽  
Vegetative Organs ◽  
Anatomical Features ◽  
Leaf Petiole ◽  
Plant Parts ◽  
Peltate Trichomes ◽  
Stem Leaf ◽  
Scanning Electron

The anatomy and micromorphology of the vegetative organs and calyx of Stachys scardica (Griseb.) Hayek were investigated using light (LM) and scanning electron microscopy (SEM). The analysis of the anatomical structure of stem, leaf and leaf petiole showed the presence of an additional adaxial phloem in the vascular bundle of the petiole that was recorded exclusively in species belonging to the subgenus Betonica. On the surface of studied plant parts, three types of trichomes were found: simple nonglandular uniseriate multicellular, nonglandular branched elongated and glandular peltate trichomes. The present study shows that certain micromorphological and anatomical features of Stachys taxa are valuable taxonomic characters.

scholarly journals Morphology, distribution, and histochemistry of trichomes of Thymus lykae Degen & Jav. (Lamiaceae)

2008 ◽  
Vol 60 (4) ◽  
pp. 667-672 ◽  
Author(s):  
Marija Marin ◽  
Snezana Budimir ◽  
Dusica Janosevic ◽  
P.D. Marin ◽  
Sonja Duletic-Lausevic ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Laser Scanning ◽  
Glandular Trichomes ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Capitate Trichomes ◽  
Histochemical Tests ◽  
Scanning Electron

Micromorphology, distribution, and histochemistry of the trichomes of Thymus lykae were studied using scanning electron microscopy (SEM)and confocal laser scanning electron microscopy (CLSM). The leaves, stem, and calyx bear numerous glandular and non-glandular trichomes. Two types of glandular trichomes are identified - peltate and capitate. Results of histochemical tests showed positive reactions to polysaccharides, proteins, and lipids. Yellow autofluorescence of secreted material was noticed in peltate and capitate trichomes. Strong reddish-yellow autofluorescence of the lipophilic and hydrophilic secreted material was observed with CLSM.

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