scholarly journals Auxins Regulations of Branched Spike Development and Expression of TFL, a LEAFY-Like Gene in Branched Spike Wheat (Triticum aestivum)

2017 ◽  
Vol 9 (2) ◽  
pp. 27
Author(s):  
Wang Yue ◽  
Sun Fulai ◽  
Gao Qingrong ◽  
Zhang Yanxia ◽  
Wang Nan ◽  
...  
Keyword(s):  
Photosynthetic Pigment ◽  
Dry Mass ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Branched Spike ◽  
Spike Development ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
The Impact ◽  
Indole 3 Acetic Acid ◽  
Putative Homologue

Branched spike wheat is a hexaploid germplasm with branched rachis on its main rachises, and the crucial period for branched rachises occurrence and development is just after the two ridges stage of shoot apex. Natural [indole-3-acetic acid (IAA), indole-3butyric acid (IBA)] and synthetic [(1-naphthaleneacetic acid (NAA), 2,4-Dichlorophenoxyacetic acid (2,4-D)] auxins were applied at this period to investigate the spike traits, seedling growth and photosynthesis related characters and expression of a putative homologue of the LEAFY in branched spike wheat. The four types of experienced auxins induced similar effects on these foresaid characters, although the impact extents were different among the auxins treatments. More branched rachis, spikelets, fertile florets and longer branched rachis were obtained in plants with IAA and IBA at 0.1 mM or NAA and 2,4-D at 1.0mM than those plants with no auxin treated. Auxin treatments also increased fresh and dry mass, photosynthetic pigment and parameters. TFL, a LEAFY-like gene was cloned in branched spike wheat and TFL mRNA expression was quantified using real-time reverse transcriptase-PCR. Application of the auxins accelerated the rise in TFL expression during the periods of branched rachises occurrence and extension. The data supports the hypothesis that auxins play a central role in the regulation branched spike development and TFL might correlate with the development of branched rachises in branched spike wheat.

scholarly journals Production of callus mediated gynogenic haploids in winter squash (Cucurbita maxima Duch.) and pumpkin (Cucurbita moschata Duch.)

2018 ◽  
Vol 54 (No. 1) ◽  
pp. 9-16 ◽  
Author(s):  
E.S. Kurtar ◽  
A. Balkaya ◽  
M. Ozbakir Ozer
Keyword(s):  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Cucurbita Moschata ◽  
Cucurbita Maxima ◽  
Culture Time ◽  
Irradiated Pollen ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Winter Squash ◽  
Indole 3 Acetic Acid

Although haploids were successfully produced via irradiated pollen technique and anther culture in Cucurbita maxima and Cucurbita moschata, the haploidization efficiency is still low due to genotype dependence. Thus, as an alternative technique, the efficacy of the ovule culture was investigated. Ovules were extracted at different flowering time and then cultured on a solid MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP), thidiazuron (TDZ), and naphthaleneacetic acid (NAA) to induce callogenesis and plant regeneration. The gynogenic response was influenced by the combination of plant growth regulators, genotype and culture time. The medium containing of 4.0 mg/l BAP + 0.05 mg/l NAA + 0.1 mg/l TDZ provided the highest response at anthesis time. Plantlets were rooted and elongated on a solid MS medium supplemented with 0.01 mg/l indole-3-acetic acid (IAA) + 1.0 mg/l BAP. The ploidy observations of 122 plants revealed that 70 plants were haploid, 46 plants were diploid and the others were mixoploid.  

The Effect of Auxins and Antiauxins on Shoot-Bud Induction and Morphology in the Moss, Bryum atrovirens Will ex Brid

1990 ◽  
Vol 38 (2) ◽  
pp. 177 ◽  
Author(s):  
RN Chopra ◽  
BD Vashistha
Keyword(s):  
Basal Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Simultaneous Application ◽  
Shoot Bud ◽  
Cultural Conditions ◽  
Bud Induction ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid ◽  
Do So

The protonema of the moss Bryum atrovirens remains bud-free under ordinary cultural conditions on Nitsch's basal medium. Exogenously applied auxins (Indole-3-acetic acid; 2-4-dichlorophenoxyacetic acid; α-naphthaleneacetic acid and β-naphthoxyacetic acid) induced buds on protonemata, whereas antiauxins (Maleic hydrazide and 2,3,5-triiodobenzoic acid) failed to do so. Morphology of the gametophores depended upon the concentration of auxin in the medium. In general, normal leafy gametophores resulted at lower concentrations, and at higher levels of auxins morphology was adversely affected. Simultaneous application of 6-benzylaminopurine and 2,4-dichlorophenoxyacetic acid advanced bud formation as well as increased bud number, but had no significant effect on the improvement of shoot morphology.

Selectivity of auxin for induction and growth of callus from excised embryo of spring and winter wheat

1984 ◽  
Vol 62 (7) ◽  
pp. 1393-1397 ◽  
Author(s):  
M. D. Zhou ◽  
T. T. Lee
Keyword(s):  
Winter Wheat ◽  
Chinese Spring ◽  
Shoot Growth ◽  
Callus Formation ◽  
Triticum Aestivum L ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Methoxybenzoic Acid

The callus-promoting activity of most commonly known as well as some rarely tested auxins was compared with that of 2,4-dichlorophenoxyacetic acid (2,4-D) for in vitro culture of the excised embryo of spring and winter wheat (Triticum aestivum L.), cv. Chinese Spring and cv. Fredrick. Different auxins in a concentration range from 1 to 50 μM showed widely different activities. Also the two wheat cultivars responded differently to the auxins. When rapid callus formation with limited root growth was used as the basis for comparison, 2-(2-methyl-4-chlorophenoxy)propionic acid (2-MCPP), α-naphthaleneacetic acid, 3,6-dichloro-2-methoxybenzoic acid (dicamba), 4-amino-3,5,6,trichloropicolinic acid (picloram), γ-(2,4-dichlorophenoxy)butyric acid, 2,4,5-trichlorophenoxyacetic acid, and 2,4,5-trichlorophenoxypropionic acid, in the order of effectiveness, were superior to 2,4,-D for callus induction from the embryo of 'Chinese Spring,' although the concentration required was higher than that of 2,4-D. For the winter wheat 'Fredrick,' however, only picloram, dicamba, and 2-MCPP performed as well as 2,4-D. All auxins tested promoted shoot growth; 2-methyl-4-chlorophenoxypropionic acid was most effective for 'Chinese Spring,' whereas picloram was most effective for 'Fredrick.'

scholarly journals Response of pine hypocotyl sections to growth regulators and related substances

2015 ◽  
Vol 44 (1) ◽  
pp. 123-132
Author(s):  
J. Zakrzewski
Keyword(s):  
Acetic Acid ◽  
Growth Regulators ◽  
Growth Response ◽  
Extended Period ◽  
Dichlorophenoxyacetic Acid ◽  
Pinus Silvestris ◽  
Related Substances ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid ◽  
Synthetic Growth

Growth response of <i>Pinus silvestris</i> hypocotyl sections to some synthetic growth regulators and related substances was studied. Elongation of hypocotyl sections was stimulated by naphtaleneacetic acid, indole-3-acetic acid, in-dole-3-propionic acid, indole-3-butyric acid, 2,4-dichlorophenoxyacetic acid, indoleaoetic amide, indoleacetic nitrile and coumarin. Indole-3-acetic acid and naphtaleneacetic acid extended period of growth up to 16 and 24 hours, respectively. Growth was inhibited by kinetin, trans-cinnamic acid and 2,3,5-tri-iodobenzoic acid. No effect of gibberellic acid, tryptophan and biotin was observed.

scholarly journals Changes in Leaf-Sugar Content and Enzyme Activity of Immature Sugarcane Following Foliar Application of Indole-3-Acetic Acid, 2,4-Dichlorophenoxyacetic Acid, and Maleic Hydrazide

1969 ◽  
Vol 49 (1) ◽  
pp. 1-34
Author(s):  
Alex G. Alexander
Keyword(s):  
Acetic Acid ◽  
Maleic Hydrazide ◽  
Foliar Application ◽  
Sugar Content ◽  
Water Soluble ◽  
Dichlorophenoxyacetic Acid ◽  
Enzyme Preparations ◽  
Freeze Dried ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid

Indole-3-acetic acid, 2,4-dichlorophenoxyacetic acid, and maleic hydrazide were applied as foliar sprays to 10-week-old sugarcane plants during initial studies of the interrelationships of growth-regulating materials with the sugar-metabolizing enzymes of sugarcane. Leaf samples were harvested at 1, 3, 9, and 27 days following treatment for sugar and enzyme assays. Sugar analyses were run for total ketoses, sucrose, fructose, and total reducing sugars, with glucose being determined by calculation. A series of acid phosphatase assays were conducted using as substrates the following compounds: ß-glycerophosphate, adenosinetriphosphate, uridine diphosphate glucose, glucose-1-phosphate, glucose-6-phosphate, fructose-6- phosphate, fructose-1,6-diphosphate, and 3-phosphoglyceric acid. Additional enzymes included invertase, amylase, hexokinase, phosphohexose isomerase, aldolase, triosephosphate dehydrogenase, phosphoglyceryl kinase, condensing enzyme, isocitric acid dehydrogenase, transaminase, peroxidase, and glucose oxidase. All enzyme preparations consisted of dialyzed water-soluble protein extracted from freeze-dried leaf tissue and precipitated with ammonium sulfate between 35 and 95 percent of saturation.

scholarly journals Callogenesis in Cicer arietinum and identification of a genotype resistant to Ascochyta rabiei

2020 ◽  
Vol 12 (3) ◽  
pp. 673-682
Author(s):  
Yasmina BENABDESSLEM ◽  
Kadda HACHEM ◽  
Samia GHOMARI
Keyword(s):  
Cicer Arietinum ◽  
Crop Yields ◽  
High Sensitivity ◽  
Ascochyta Rabiei ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Fruiting Bodies ◽  
Callus Tissues ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Indole 3 Acetic Acid

The chickpea (Cicer arietinum) is one of the leguminous species most appreciated by consumers in the Mediterranean basin, while being an important source of protein. Nevertheless, its crop yields are greatly limited by several biotic and abiotic stresses, the main one being Ascochyta rabiei, the causal agent of anthracnose. As traditional breeding methods have proved to be ineffective in controlling this pathogen, resorting to biotechnological methods is necessary. Therefore, in this study, the callogenic capacity of stem and leaflet explants from three genotypes of chickpea, namely ‘FLIP 84-92 C’, ‘ILC 32-97’, and ‘ILC 263’, cultured on Murashige and Skoog (MS) medium with different hormonal balances of auxins (indole-3-acetic acid [IAA] and 2,4-dichlorophenoxyacetic acid [2,4-D]) and cytokinin (kinetin), was determined. For all the genotypes, high percentages of callogenesis were recorded in the different explants grown on an MS medium with 2 mg of both IAA and kinetin. Then, a patho-system of Cicer arietinum calluses with Ascochyta rabiei was investigated, followed by a histological assessment of this interaction. The presence of the fruiting bodies of the pathogen was revealed in the calluses of the ‘ILC 32-97’ and ‘ILC 263’ genotypes. Notably, the latter showed a high sensitivity to the pathogen, as indicated by an abundance of pycnidia in its tissues. As for the ‘FLIP 84-92 C’ genotype, the histological sections showed a total absence of inter- and intracellular fruiting bodies of the pathogen in the callus tissues. Therefore, this genotype was considered as resistant to Ascochyta rabiei.

scholarly journals Impact of Ionic Liquids on Induction of Wheat Microspore Embryogenesis and Plant Regeneration

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 839
Author(s):  
Dorota Weigt ◽  
Idzi Siatkowski ◽  
Magdalena Magaj ◽  
Agnieszka Tomkowiak ◽  
Jerzy Nawracała
Keyword(s):  
Ionic Liquids ◽  
Plant Regeneration ◽  
Positive Impact ◽  
Microspore Embryogenesis ◽  
Green Plant ◽  
Induction Medium ◽  
Dichlorophenoxyacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
The Impact

Ionic liquids are novel compounds with unique chemical and physical properties. They can be received based on synthetic auxins like 2,4-dichlorophenoxyacetic acid or dicamba, which are commonly used hormones in microspore embryogenesis. Nevertheless, ionic liquids have not been adapted in plant in vitro culture thus far. Therefore, we studied the impact of ionic liquids on the ability to undergo microspore embryogenesis in anther cultures of wheat. Two embryogenic and two recalcitrant genotypes were used for this study. Ten combinations of ionic liquids and 2,4-dichlorophenoxyacetic acid were added to the induction medium. In most cases, they stimulated induction of microspore embryogenesis and green plant regeneration more than a control medium supplemented with only 2,4-dichlorophenoxyacetic acid. Two treatments were the most favorable, resulting in over two times greater efficiency of microspore embryogenesis induction in comparison to the control. The effect of breaking down the genotype recalcitrance (manifested by green plant formation) was observed under the influence of 5 ionic liquids treatments. Summing up, ionic liquids had a positive impact on microspore embryogenesis induction and green plant regeneration, increasing the efficiency of these phenomena in both embryogenic and recalcitrant genotypes. Herbicidal ionic liquids can be successfully used in in vitro cultures.

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