Speciation and Determination of Inorganic Arsenic in Rice Using Liquid Chromatography-Inductively Coupled Plasma/Mass Spectrometry: Collaborative Study

2014 ◽  
Vol 97 (3) ◽  
pp. 946-955 ◽  
Author(s):  
Takanori Ukena ◽  
Eri Matsumoto ◽  
Tsutomu Nishimura ◽  
Joanne Chan Sheot Harn ◽  
Ch'ng Ai Lee ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Collaborative Study ◽  
Inorganic Arsenic ◽  
Dimethylarsinic Acid ◽  
Inductively Coupled ◽  
Monomethylarsonic Acid ◽  
Inorganic As ◽  
Plasma Mass Spectrometry ◽  
Rice Samples

Abstract An analytical method to speciate two inorganic As forms [arsenite, As(III) and arsenate, As(V)] in indica and japonica types of rice (both husked and polished) and determine the inorganic As concentration as the sum of these two was internationally validated. The method can additionally determine two organic As compounds, monomethylarsonic acid and dimethylarsinic acid, in rice as separate LC peaks. The method is based on LC separation and inductively coupled plasma (ICP)-MS detection. The method was evaluated through the International Union of Pure and Applied Chemistry/International Organization for Standardization/AOAC harmonized protocol. Sixteen laboratories from four countries participated in the study, and 13 laboratories returned valid data. Twenty test portions of 10 blind duplicates of indica and japonica type rice samples (both husked and polished) were used in this study. Repeatability RSD (RSDr) and reproducibility RSD (RSDR) were calculated at five concentrations of total inorganic As between 0.03 and 0.68 mg/kg. The RSDr was in a range of 3.8 to 7.7% and the RSDR was in a range of 10 to 36%. These performance characteristics were found to be sufficient for determination of inorganic As at or higher than 0.03 mg/kg. Applicability of the method was estimated to be in a range of 0.02–2.0 mg/kg.

scholarly journals Pharmacokinetic Properties of Arsenic Species after Intravenous and Intragastrical Administration of Arsenic Trioxide Solution in Cynomolgus Macaques Using HPLC-ICP-MS

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 241 ◽  
Author(s):  
Qiaoli Shi ◽  
Mingyan Ju ◽  
Xiaoxia Zhu ◽  
Hui Gan ◽  
Ruolan Gu ◽  
...  
Keyword(s):  
Arsenic Trioxide ◽  
Inductively Coupled Plasma ◽  
High Performance ◽  
Cynomolgus Macaque ◽  
Arsenic Species ◽  
Dimethylarsinic Acid ◽  
Inductively Coupled ◽  
Monomethylarsonic Acid ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

A rapid and sensitive method was established for arsenic (As) speciation based on high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (HPLC-ICP-MS). This method was validated for the quantification of four arsenic species, including arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMAV) and dimethylarsinic acid (DMAV) in cynomolgus macaque plasma. Separation was achieved in just 3.7 min with an alkyl reverse phase column and highly aqueous mobile phase containing 20 mM citric acid and 5 mM sodium hexanesulfonate (pH = 4.3). The calibration curves were linear over the range of 5–500 ng·mL−1 (measured as As), with r > 0.99. The above method was validated for selectivity, precision, accuracy, matrix effect, recovery, carryover effect and stability, and applied in a comparative pharmacokinetic study of arsenic species in cynomolgus macaque samples following intravenous and intragastrical administration of arsenic trioxide solution (0.80 mg·kg−1; 0.61 mg·kg−1 of arsenic); in addition, the absolute oral bioavailability of the active ingredient AsIII of arsenic trioxide in cynomolgus macaque samples was derived as 60.9 ± 16.1%.

scholarly journals Stability of inorganic arsenic species in simulated raw waters with the presence of NOM

2006 ◽  
Vol 6 (6) ◽  
pp. 175-182 ◽  
Author(s):  
G.J. Liu ◽  
X.R. Zhang ◽  
J. Jain ◽  
J.W. Talley ◽  
C.R. Neal
Keyword(s):  
Natural Organic Matter ◽  
Inductively Coupled Plasma ◽  
Inorganic Arsenic ◽  
Arsenic Species ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Mass Spectrometry System ◽  
The Stability ◽  
Inorganic Arsenic Species

Effect of natural organic matter (NOM) on the stability of inorganic arsenic species in simulated raw water was examined at circumneutral pH. An ion chromatography–inductively coupled plasma mass spectrometry system was used for simultaneous determination of As(III) and As(V). A reduction of arsenate (As(V)) to arsenite (As(III)) was observed in the unfiltered simulated raw waters (USW). The As(V) reduction to As(III) did not occur in the simulated waters that passed through a 0.2 μm membrane (FSW). Microorganism activities is probably the major reason causing As(V) reduction in the USW. In the FSW without NOM, As(III) tended to be oxidized into As(V). The addition of 0.036 mM of Fe(II) significantly facilitated the oxidation. The presence of 10 mg/L Suwannee River NOM as C inhibited As(III) oxidation no matter whether Fe(II) existed or not. The experimental results suggest that NOM can mediate distribution of inorganic arsenic species in water, thus it is an important factor controlling the mobility and toxicity of arsenic in drinking water.

scholarly journals Cyanobacteria produce arsenosugars

2012 ◽  
Vol 9 (5) ◽  
pp. 474 ◽  
Author(s):  
Shin-ichi Miyashita ◽  
Shoko Fujiwara ◽  
Mikio Tsuzuki ◽  
Toshikazu Kaise
Keyword(s):  
Inductively Coupled Plasma ◽  
High Performance ◽  
Inorganic Arsenic ◽  
Freshwater Ecosystems ◽  
Dimethylarsinic Acid ◽  
Arsenic Compounds ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Synechocystis Sp ◽  
Pcc 6803

Environmental contextAlthough arsenic is known to accumulate in both marine and freshwater ecosystems, the pathways by which arsenic is accumulated and transferred in freshwater systems are reasonably unknown. This study revealed that freshwater cyanobacteria have the ability to produce arsenosugars from inorganic arsenic compounds. The findings suggest that not only algae, but cyanobacteria, play an important role in the arsenic cycle of aquatic ecosystems. AbstractMetabolic processes of incorporated arsenate in axenic cultures of the freshwater cyanobacteria Synechocystis sp. PCC 6803 and Nostoc (Anabaena) sp. PCC 7120 were examined. Analyses of arsenic compounds in cyanobacterial extracts using a high-performance liquid chromatography–inductively coupled plasma mass spectrometry system showed that both strains have an ability to biotransform arsenate into oxo-arsenosugar-glycerol within 20 min through (1) reduction of incorporated arsenate to arsenite and (2) methylation of produced arsenite to dimethylarsinic acid by methylarsonic acid as a possible intermediate product. In addition, Synechocystis sp. PCC 6803 cells are able to biosynthesise oxo-arsenosugar-phosphate from incorporated arsenate. These findings suggest that arsenosugar formation as well as arsenic methylation in cyanobacteria possibly play a significant role in the global arsenic cycle.

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