scholarly journals Determination of Inorganic Arsenic in Seaweed and Seafood by LC-ICP-MS: Method Validation

2019 ◽  
Vol 102 (2) ◽  
pp. 612-618 ◽  
Author(s):  
Eri Matsumoto-Tanibuchi ◽  
Toshiaki Sugimoto ◽  
Toshiyuki Kawaguchi ◽  
Naoki Sakakibara ◽  
Michiaki Yamashita
Keyword(s):  
Brown Algae ◽  
Inductively Coupled Plasma ◽  
Mobile Phase ◽  
Arsenic Speciation ◽  
Inorganic Arsenic ◽  
Food Samples ◽  
Ion Pair ◽  
Arsenic Compounds ◽  
Intermediate Precision ◽  
Icp Ms

Abstract Background: Seaweed and seafoodoften contain both inorganic and organic arsenic compounds showing distinct toxicities. Speciation must be taken into account when determining the concentrations of arsenic compounds and how they relate to overall toxicity. Objective: An analytical method for the quantitation of inorganicarsenic was validated in seaweed and seafood. Methods: Food samples were heated at 100°C in 0.3 mol/L nitric acid. Arsenic speciation was quantitatively determined by LC-inductively coupled plasma-MS (LC-ICP-MS) using an octadecilsilane (ODS) column with a mobile phase containing an ion-pair reagent. Results: Limits of detection (0.0023–0.012 mg/kg), LOQ (0.0077–0.042 mg/kg), repeatability (3.0–7.4%), intermediate precision (4.4–7.4%), and trueness (recoveries 94–107%) of the proposed method were satisfactory for inorganic arsenicin seaweed and seafood. Inorganic arsenic was detected in almost all the evaluated dried seaweed products, the Japanese oyster, nam pla, oyster sauce, and the intestinal organs of seafood. Conclusions: Among the dried seaweed products, significant inorganic arsenic was detected in the brown algae akamoku, hijiki, and mozuku. The small amounts of inorganic arsenic detected in nam pla and oyster sauce likely derive from the internal organs of the raw seafood used in their preparation. Highlights: Arsenic speciation in seaweed and seafood was measured by LC-ICP-MS using an ODS column with a mobile phase containing an ion-pair reagent. Among the dried seaweed products, brown algae akamoku, hijiki, and mozuku contained significantly high levels of inorganic arsenic. The intestinal organs of oyster, sardine, and scallop contained higher arsenic levels than the muscles.

Determination of Inorganic Arsenic in Fish Oil and Fish Oil Capsules by LC-ICP-MS

2020 ◽  
Author(s):  
Eri Matsumoto ◽  
Toshiaki Sugimoto ◽  
Toshiyuki Kawaguchi ◽  
Naoki Sakakibara ◽  
Michiaki Yamashita
Keyword(s):  
Fish Oil ◽  
Mobile Phase ◽  
Arsenic Speciation ◽  
Inorganic Arsenic ◽  
Ion Pair ◽  
Tetramethylammonium Hydroxide ◽  
Intermediate Precision ◽  
Low Level ◽  
Icp Ms ◽  
Anchovy Oil

Abstract Background As inorganic arsenic is a highly toxic compound, its concentration in foods should be determined. Objective Develop an analytical method for determining inorganic arsenic in fish oil and fish oil capsules. Method Inorganic arsenic was extracted from fish oil by heating at 80°C in 1.6% tetramethylammonium hydroxide-ethanol. The concentration of inorganic arsenic in fish oil was determined by liquid chromatography (LC) inductively coupled plasma (ICP) MS using an octadecylsilane (ODS) column with a mobile phase containing an ion-pair reagent. Results The LOD (0.005, 0.004 mg/kg), LOQ (0.016, 0.011 mg/kg), repeatability (4.2, 3.5%), intermediate precision (5.4, 3.5%), and trueness (recoveries 94–109% based on spiked samples) of the proposed method were satisfactory for inorganic arsenic in fish oil and fish oil capsules. A low level of inorganic arsenic was detected only in anchovy oil among all fish oil samples that were used in this study. Inorganic arsenic levels were below the quantitation limit in all fish oil capsules. Conclusions Inorganic arsenic was determined after extraction from fish oil by heating at 80°C in 1.6% tetramethylammonium hydroxide–ethanol. The level of inorganic arsenic in all fish oil samples examined in this study was lower than 0.1 mg/kg of the maximum level defined in the Codex. Highlights Arsenic speciation in fish oil and fish oil capsules was analyzed by LC-ICP-MS using an ODS column with a mobile phase containing an ion-pair reagent. A low level of inorganic arsenic was detected only in anchovy oil. No inorganic arsenic was detected in fish oil capsules.

Speciation of Six Arsenic Compounds in Korean Seafood Samples by HPLC-ICP-MS

2005 ◽  
Vol 277-279 ◽  
pp. 431-437 ◽  
Author(s):  
Kyung Su Park ◽  
Jeong Sook Kim ◽  
Hyo Min Lee ◽  
Hee Soo Pyo ◽  
Soon Tae Kim ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
High Performance ◽  
Certified Reference Materials ◽  
Arsenic Speciation ◽  
Inorganic Arsenic ◽  
Arsenic Species ◽  
Arsenic Compounds ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Fish And Shellfish

Extracts of 33 samples of seaweed, shrimp, fish and shellfish, including two certified reference materials, were investigated for their contents of arsenic compounds (arsenic speciation).An anion exchange high performance liquid chromatography procedure was optimized to separate six arsenic compounds present in the seafood samples with dynamic reaction gas cell by inductively coupled plasma mass spectrometry. The concentration of each species in the sample were: arsenobetaines - 0.019-1.04 mg/kg, arsenocholine - 0.033-69.0 mg/kg, arseniousacid - ND-1.25 mg/kg, dimethylarsinate - ND-3.75 mg/kg, monomethylarsonate - ND-8.33 mg/kg, arsenic acid - ND-0.55 mg/kg. Additionally, unknown arsenic species were present in most of samples. The intake of inorganic arsenic via ingestion of the seafood samples that were analyzed did not represent a toxicological problem to humans. The limits of detection (LOD) were in the range of 0.5-2.5 µg/kg .

Arsenic speciation in rice and risk assessment of inorganic arsenic from Ghentugachhi village of Chakdaha block, Nadia, West Bengal, India

2020 ◽  
Vol 57 (2) ◽  
pp. 85-93
Author(s):  
B Sinha ◽  
K Bhattacharyya
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
West Bengal ◽  
Arsenic Speciation ◽  
Inorganic Arsenic ◽  
Rice Grain ◽  
Species Determination ◽  
Inductively Coupled ◽  
As Species ◽  
Plasma Mass Spectrometry

The purpose of the present study was to assess arsenic (As) speciation in rice from West Bengal, India, in order to improve understanding of the health risk posed by arsenic in Indian rice. Rice is a potentially important route of human exposure to arsenic, especially in populations with rice-based diets. However, arsenic toxicity varies greatly with species. Determination of arsenic (As) species in rice is necessary because inorganic As species are more toxic than organic As. Total arsenic was determined by inductively coupled plasma mass spectrometry; arsenite, arsenate, monomethylarsonic acid, and dimethyarsinic acid were quantified by high-performance liquid chromatography- inductively coupled plasma mass spectrometry. The analysis of a rice flour certified reference material (SRM-1568-a) were evaluated for quality assurance. The use of 2M TFA for extraction with an isocratic mobile phase was optimized for extraction and employed for arsenic speciation in rice. The extraction method showed a high recovery of arsenic. Most of the As species in rice were noticed to be inorganic [Arsenite (As-III), Arsenate As-V]. It appeared very clear from the present study that inorganic arsenic shared maximum arsenic load in rice straw while in grains it is considerably low. As species recovered from rice grain and straw are principally As-III and As-V with a little share of DMA and almost non-detectable MMA and As-B. The order of As species in rice grain revealed in this study were As-III (54.5-65.4 %)>As-V(21.2-28.3%)>DMA(5.2%).

scholarly journals Arsenic speciation in food chains from mid-Atlantic hydrothermal vents

2012 ◽  
Vol 9 (2) ◽  
pp. 130 ◽  
Author(s):  
Vivien F. Taylor ◽  
Brian P. Jackson ◽  
Matthew R. Siegfried ◽  
Jana Navratilova ◽  
Kevin A. Francesconi ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Deep Sea ◽  
Hydrothermal Vents ◽  
Arsenic Speciation ◽  
Inorganic Arsenic ◽  
Food Sources ◽  
Arsenic Compounds ◽  
Marine Animals ◽  
Δ13c And Δ15n ◽  
Organic Arsenic

Environmental contextArsenic occurs in marine organisms at high levels and in many chemical forms. A common explanation of this phenomenon is that algae play the central role in accumulating arsenic by producing arsenic-containing sugars that are then converted into simpler organic arsenic compounds found in fish and other marine animals. We show that animals in deep-sea vent ecosystems, which are uninhabited by algae, contain the same organic arsenic compounds as do pelagic animals, indicating that algae are not the only source of these compounds. AbstractArsenic concentration and speciation were determined in benthic fauna collected from the Mid-Atlantic Ridge hydrothermal vents. The shrimp species, Rimicaris exoculata, the vent chimney-dwelling mussel, Bathymodiolus azoricus, Branchipolynoe seepensis, a commensal worm of B. azoricus and the gastropod Peltospira smaragdina showed variations in As concentration and in stable isotope (δ13C and δ15N) signature between species, suggesting different sources of As uptake. Arsenic speciation showed arsenobetaine to be the dominant species in R. exoculata, whereas in B. azoricus and B. seepensis arsenosugars were most abundant, although arsenobetaine, dimethylarsinate and inorganic arsenic were also observed, along with several unidentified species. Scrape samples from outside the vent chimneys covered with microbial mat, which is a presumed food source for many vent organisms, contained high levels of total As, but organic species were not detectable. The formation of arsenosugars in pelagic environments is typically attributed to marine algae, and the pathway to arsenobetaine is still unknown. The occurrence of arsenosugars and arsenobetaine in these deep sea organisms, where primary production is chemolithoautotrophic and stable isotope analyses indicate food sources are of vent origin, suggests that organic arsenicals can occur in a foodweb without algae or other photosynthetic life.

Ionic imprinted polymer – Vortex-assisted dispersive micro-solid phase extraction for inorganic arsenic speciation in rice by HPLC-ICP-MS

Talanta ◽  
2020 ◽  
Vol 220 ◽  
pp. 121418
Author(s):  
Kamal K. Jinadasa ◽  
Elena Peña-Vázquez ◽  
Pilar Bermejo-Barrera ◽  
Antonio Moreda-Piñeiro
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Arsenic Speciation ◽  
Inorganic Arsenic ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Icp Ms

Speciation Analysis of Arsenic Compounds in Healthy Human Urine by HPLC-ICP-MS for the Evaluation of the Occupational Inorganic Arsenic Exposure

Epidemiology ◽  
2009 ◽  
Vol 20 ◽  
pp. S154-S155 ◽  
Author(s):  
Yoshihiro Suzuki ◽  
Yasuyo Shimoda ◽  
Yoko Endo ◽  
Akihisa Hata ◽  
Kenzo Yamanaka ◽  
...  
Keyword(s):  
Human Urine ◽  
Arsenic Exposure ◽  
Inorganic Arsenic ◽  
Speciation Analysis ◽  
Arsenic Compounds ◽  
Healthy Human ◽  
Icp Ms

A sequential solid phase microextraction system coupled with inductively coupled plasma mass spectrometry for speciation of inorganic arsenic

2014 ◽  
Vol 6 (12) ◽  
pp. 4205-4211 ◽  
Author(s):  
Peng Li ◽  
Xiao-qin Zhang ◽  
Yi-jun Chen ◽  
Hong-zhen Lian ◽  
Xin Hu
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Inorganic Arsenic ◽  
Inductively Coupled ◽  
Monolithic Capillary Columns ◽  
Icp Ms ◽  
Separation And Preconcentration ◽  
Plasma Mass Spectrometry

A sequential solid phase microextraction (SPME) system consisting of two monolithic capillary columns was developed for simultaneous separation and preconcentration of inorganic arsenic, followed by inductively coupled plasma mass spectrometry (ICP-MS) detection.

Determination of Arsenic (III) and Arsenic (V) Species by Preconcentration and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) in Drinking Water Samples Analysis

2011 ◽  
Vol 264-265 ◽  
pp. 1684-1689 ◽  
Author(s):  
M.M. Rahman ◽  
Alias Mohd Yusof ◽  
A.K.H. Wood ◽  
A. Shamsiah
Keyword(s):  
Mass Spectrometry ◽  
Solvent Extraction ◽  
Water Samples ◽  
Inductively Coupled Plasma ◽  
Inorganic Arsenic ◽  
Arsenic Species ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Dissolved Inorganic Arsenic

A simple and less expensive solvent extraction method was used with inductively coupled plasma mass spectrometry (ICP-MS) in the speciation of two environmentally significant, toxic forms of arsenic: arsenite and arsenate. Dissolved inorganic arsenic species in drinking waters from reservoirs and treatment plants were determined by the inductively coupled plasma mass spectrometry analyses. Prior to the analysis the water samples were precocentrated by solvent extraction using APCDT to separate the arsenic species from elemental interferences. The detection limit of this method achieved was 0.059 gL-1. The suitability of the technique in this work is discussed in relation to risk assessment studies of public health.

scholarly journals Routine determination of inorganic arsenic speciation in precipitates from acid mine drainage using orthophosphoric acid extraction followed by HPLC-ICP-MS

2016 ◽  
Vol 8 (40) ◽  
pp. 7420-7426 ◽  
Author(s):  
Resongles E. ◽  
Le Pape P. ◽  
Fernandez-Rojo L. ◽  
Morin G. ◽  
Delpoux S. ◽  
...  
Keyword(s):  
Acid Mine Drainage ◽  
Orthophosphoric Acid ◽  
Mine Drainage ◽  
Arsenic Speciation ◽  
Inorganic Arsenic ◽  
Acid Extraction ◽  
Acid Mine ◽  
Routine Measurement ◽  
Icp Ms

As(iii)/As(v) ratio determined from orthophosphoric acid extraction/HPLC-ICP-MS matched XANES results, allowing routine measurement of As oxidation state in acid mine drainage precipitates.

scholarly journals Study of toxicity of arsenic-containing compounds isolated from brown algae Saccharina japonica in laboratory animals

Trudy VNIRO ◽  
2020 ◽  
Vol 181 ◽  
pp. 223-234
Author(s):  
L.S. Abramova ◽  
◽  
V.V. Gershunskaya ◽  
A.V. Kozin ◽  
D.A. Bondarenko ◽  
...  
Keyword(s):  
Brown Algae ◽  
Inorganic Arsenic ◽  
Laminaria Japonica ◽  
Saccharina Japonica ◽  
Raw Material ◽  
Toxic Effects ◽  
Laboratory Animals ◽  
Arsenic Content ◽  
Total Arsenic ◽  
Arsenic Compounds

The ability of various marine organisms, especially algae and invertebrates, to accumulate arsenic in high concentrations can pose a threat to public health when consumed. It is known from the literature that inorganic arsenic compounds (arsenites and arsenates) are the most toxic, in comparison with methylated forms of the element, and especially with complex organic compounds (arsenobetain, arsenocholine, tetramethylarsonium, arsenoriboses), which are considered non-toxic for live organisms. Monitoring of safety indicators of aquatic biological resources in the main commercial basins of the Russian Federation has shown that the most common excess of total arsenic content is characteristic for algae. According to TR CU 021/2011, the total arsenic content in algae should be 5 mg / kg and the established norm without separation of organic and inorganic arsenic compounds creates a barrier to the rational use of seafood. In this regard, the justification of the norms for the content of inorganic arsenic in algae and the assessment of their toxicity is a very urgent problem. Study of the samples of commercial brown algae Saccharina (=Laminaria) japonica and its derivates with ICP-MS, HPLC–MS-ISP methods, the maximum permissible level of arsenic was found to be exceeded, but the most toxic inorganic forms made up from 6 to14 % of the total amount of arsenic in the raw material. Acute toxicity on laboratory animals (rats) was studied and the absence of toxic effects was shown when an oral suspension containing high doses of arsenic was administered. Repeated administration of the same substances to laboratory mice of the CD 1 line has shown no toxic effects even after multiple doses of arsenic isolated from algae.

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