Modified Magnetic Nanoparticle-Based Solid-Phase Extraction for the Determination of Trace Amounts of Aflatoxins B1 and B2 in Chinese Patent Medicines: The Use of Fupuganmao Granules as a Case Study

2019 ◽  
Vol 102 (3) ◽  
pp. 761-766 ◽  
Author(s):  
Qing Li ◽  
Luyi Jiang ◽  
Huan Zhang ◽  
Mengmeng Wei ◽  
Chu Chu ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Magnetic Nanoparticle ◽  
Solid Phase ◽  
Low Cost ◽  
Phase Extraction ◽  
Chromatography Method ◽  
Patent Medicines ◽  
Functionalized Magnetic Nanoparticles ◽  
Chinese Patent

Abstract Background: Aflatoxins (AFs) have attracted increasing amounts of attention in recent years for their high toxicity. Previously, AF-polluted Chinese Patent Medicines (CPMs) were ignored, but their quality, safety, and efficacy might be influenced by the toxic compounds. Objective: The objective is to develop a simple, low-cost, and efficient method for quantitative analysis of aflatoxins B1 (AFB1) and B2 (AFB2) in CPMs to ensure their safe use. Methods: A simple, modified, magnetic nanoparticle-based solid-phase extraction (SPE) combined with HPLC-fluorescence detection for the determination of trace amounts of AFB1 and AFB2 was established. The main parameters affecting the efficiency of modified magnetic nanoparticle-based SPE, such as pH of sample solution, adsorbent amount, adsorption time, and desorption condition, were investigated. Results: Under the optimum conditions, AFB1 and AFB2 were linear in the ranges of 0.3–10 and 0.04–3 ng/mL with the correlation coefficient (R) of 0.9998 and 0.9999, respectively. Their intraday precisions were 1.16 and 2.30% and the interday precisions were 1.28 and 1.87% for AFB1 and AFB2, respectively. The developed SPE was applied for AFB1 and AFB2 extraction in three commercially available Fupuganmao granule samples, and the results were compared with the official immunoaffinity column chromatography method. Conclusions: The method provided a preferable candidate for the determination approach of AF measurement in CPMs. Highlights: Amine-functionalized magnetic nanoparticles were successfully applied to SPE for adsorbing AFB1 and AFB2 in CPMs for the first time.

A Simple and Sensitive Dispersive Micro-Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography for Quantification of Honokiol and Magnolol in Complex Matrices

2020 ◽  
Vol 103 (5) ◽  
pp. 1406-1411
Author(s):  
Chu Chu ◽  
Jing Li ◽  
Shan Wang ◽  
Lvnan Weng ◽  
Luyi Jiang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
High Performance ◽  
Solid Phase ◽  
Water Soluble ◽  
Phase Extraction ◽  
Patent Medicines ◽  
Chinese Patent

Abstract Background Honokiol and magnolol were considered as markers for the analysis of Cortex Magnoliae Officinalis, its related Chinese Patent Medicines and their metabolites. However, the determination of these two analytes in a water-soluble sample is difficult and therefore requires a more efficient method. Objective To develop a sensitive method for the determination of honokiol and magnolol in a water-soluble sample for better quality control of Cortex Magnoliae Officinalis and its related Chinese Patent Medicines. Method In this work, a combination of dispersive micro-solid-phase extraction (DMSPE) and high-performance liquid chromatography (HPLC) has been developed for simultaneous preconcentration and determination of honokiol and magnolol in complex bio-samples. Several experimental factors affecting the extraction efficiency were optimized by single factor test. Results Under the optimized extraction conditions, the proposed method exhibited good linearity of not less than 0.9998, satisfactory precision with relative standard deviation of less than 1.3%, and acceptable mean recoveries of 97.3% and 101.5% for honokiol and magnolol, respectively. Furthermore, the method exhibits extremely high sensitivity with detection limits of 0.0097 and 0.0231 ng/mL, which is even more sensitive than those methods developed by MS. Conclusions The method established in this study is fast, economic, accurate, easy to operate, and importantly well suited to the extraction and analysis of honokiol and magnolol in a real complex sample matrix.

Evaluation of modified Fe3O4 magnetic nanoparticle graphene for dispersive solid-phase extraction to determine trace PAHs in seawater

2015 ◽  
Vol 7 (22) ◽  
pp. 9587-9595 ◽  
Author(s):  
Shaojun Zhang ◽  
Wanqing Wu ◽  
Qinggong Zheng
Keyword(s):  
Solid Phase Extraction ◽  
Magnetic Nanoparticle ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Fe3o4 Magnetic Nanoparticle

A novel Fe3O4 MNG@CTAB composite was prepared as a DSPE adsorbent for determination of PAH contaminants in seawater.

scholarly journals Adsorption of nitrate, nitrite, and ammonium ions on carbon adsorbents

2017 ◽  
Vol 35 (7-8) ◽  
pp. 721-727 ◽  
Author(s):  
Andrzej Gierak ◽  
Iwona Łazarska
Keyword(s):  
Solid Phase Extraction ◽  
Extraction Method ◽  
Adsorption Process ◽  
Solid Phase ◽  
Carbon Adsorbent ◽  
Carbon Adsorbents ◽  
Phase Extraction ◽  
Ammonium Ions ◽  
Chromatography Method

This work presents the results of the investigations of the adsorption process of nitrate, nitrite, and ammonium ions on carbon adsorbent with the application of the solid phase extraction method to isolate and enrich the investigated ions from aqueous solutions of the concentration below 1 mg/L. For the determination of the concentration of the analyzed ions, we applied the ion chromatography method. The sorption capacity for the tested adsorbents as well as the efficiency of the enrichment of the investigated ions were determined.

Development of an on-line solid phase extraction method based on new functionalized magnetic nanoparticles. Use in the determination of mercury in biological and sea-water samples

Talanta ◽  
2016 ◽  
Vol 153 ◽  
pp. 228-239 ◽  
Author(s):  
Elisa Vereda Alonso ◽  
M.M. López Guerrero ◽  
Pilar Colorado Cueto ◽  
José Barreno Benítez ◽  
José Manuel Cano Pavón ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Solid Phase Extraction ◽  
Water Samples ◽  
Extraction Method ◽  
Sea Water ◽  
Solid Phase ◽  
Phase Extraction ◽  
Functionalized Magnetic Nanoparticles ◽  
On Line

Magnetic nanoparticle based dispersive micro-solid-phase extraction for the determination of malachite green in water samples: optimized experimental design

2015 ◽  
Vol 39 (12) ◽  
pp. 9813-9823 ◽  
Author(s):  
Arash Asfaram ◽  
Mehrorang Ghaedi ◽  
Alireza Goudarzi ◽  
Mustafa Soylak ◽  
Sanaz Mehdizadeh Langroodi
Keyword(s):  
Experimental Design ◽  
Solid Phase Extraction ◽  
Malachite Green ◽  
Water Samples ◽  
Extraction Method ◽  
Solid Phase Microextraction ◽  
Magnetic Nanoparticle ◽  
Solid Phase ◽  
Phase Extraction

This paper presents an extraction method based on dispersive-nanoparticle-solid phase microextraction for the preliminary preconcentration and subsequent spectrophotometric determination of trace amounts of malachite green.

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