scholarly journals Cryopreservation of Embryos and Oocytes of South American Fish Species

Author(s):  
Danilo Pedro Streit ◽  
Leandro Cesar de Godoy ◽  
Ricardo Pereira Ribeiro ◽  
Darci Carlos Fornari ◽  
Melanie Digmayer ◽  
...  
Keyword(s):  
2015 ◽  
Vol 75 (1) ◽  
pp. 224-228
Author(s):  
FA. Alves-Costa ◽  
AP. Wasko

Differential Display (DD) is a technique widely used in studies of differential expression. Most of these analyses, especially those involving fish species, are restricted to species from North America and Europe or to commercial species, as salmonids. Studies related to South American fish species are underexplored. Thus, the present work aimed to describe DD technique modifications in order to improve outcomes related to the isolation of DETs (Differentially Expressed Transcripts), using Leporinus macrocephalus, a large commercially exploited South American species, as a fish design. Different DDRT-PCR approaches were applied to brain samples and the products of the reactions were analyzed on 6% polyacrylamide gels stained with 0.17% Silver Nitrate (AgNO3). The use of PCR reactions under high stringency conditions and longer oligonucleotides based on VNTR (Variable Number of Tandem Repeats) core sequences led to better results when compared to low stringency PCR conditions and the use of decamer oligonucleotides. The improved approach led to the isolation of differentially expressed transcripts on adult males and females of L. macrocephalus. This study indicates that some modifications on the DDRT-PCR method can ensure isolation of DETs from different fish tissues and the development of robust data related to this approach.


2021 ◽  
Author(s):  
Giovana Souza Branco ◽  
Lázaro Wender O. De Jesus ◽  
Monica Cassel ◽  
Chayrra Chehade ◽  
Marília de Paiva Camargo ◽  
...  

Abstract Pituitary gland morphogenesis of the adenohypophyseal (AH) cells of Astyanax lacustris are presented herein. This Characiformes species show great ecological and commercial importance, and it has been increasingly used as a biological model. The first AH cells of A. lacustris were detected at 1 dah by the immunostaining of PRL producing cells. The morphology of the gland presented changes in shape throughout the development, starting elongated but more oval at the end. The neurohypophysis was differentiated at 3 dah, along with the identification of ACTH, MSH, TSH, and FSH producing cells. Identification of the immunoreactive cells to anti-LH, anti-SL, and anti-GH antibodies occurred at 5 dah. At 20 dah, an increase in pituitary size and the presence of the pituitary stalk were observed. At 60 dah, the pituitary already had the same shape seen in adults. The ontogeny of adenohypophyseal cells in A. lacustris corroborates the heterogeneity in the appearance of these cell types in teleosts and suggests that these hormones actively participate during the early development of this species. Our results collaborate with the understanding of the morphogenesis of the hypothalamic-pituitary-gonadal axis in South American teleosts, showing essential data for the development of future studies related to pituitary morphophysiology.


2021 ◽  
Vol 14 (5) ◽  
pp. 597-605
Author(s):  
I. A. Stolbunov ◽  
V. A. Gusakov ◽  
Tran Duc Dien ◽  
Nguyen Thi Hai Thanh

Abstract The food spectrum and trophic and length–weight characteristics of an invasive species, South American suckermouth armored catfishes Pterygoplichthys spp. (Loricariidae), from lotic and lentic inland waters of Vietnam have been studied. It is found that the diet of suckermouth armored catfishes consists of plant and animal food, as well as organic detritus. According to the predominant type and pattern of feeding, suckermouth armored catfishes can be classified as detritivore gatherers (janitor). Some specimens of catfish with a high consumption of animal food have been identified. It is noted that invasive suckermouth armored catfishes can be a significant food competitor for aboriginal fish species; detritivores; and, possibly, benthophages in the lotic and lentic inland waters of Vietnam.


Author(s):  
Sara M. Pinho ◽  
Luiz H. David ◽  
Fabiana Garcia ◽  
Karel J. Keesman ◽  
Maria Célia Portella ◽  
...  

AbstractTilapia and catfish are the most popular fish species in aquaponics. However, they are not well-accepted in all markets, and finding alternative species is important in order to increase the variety of food products and meet market demands. South America has several potential fish species for aquaponics systems. Encouraging the implementation of integrated aquaculture systems by providing information about the production of South American species can help to increase the supply of high-quality food and aquaculture diversification. Thus, data for five South American fish species with potential for aquaponics were compared with existing data for the main traditional warm water species in this system, tilapia and catfish. Moreover, the degree of suitability of the novel species for these systems in terms of zootechnical performance, tolerance to water quality and nutritional composition of fish flesh were discussed. The South American species considered were jundia or silver catfish (Rhamdia quelen), yellowtail lambari (Astyanax lacustris), pacu (Piaractus mesopotamicus), tambaqui (Colossoma macropomum) and snook (Centropomus spp.). Their description and the tabular comparison with the most traditional aquaponic-cultured species show they are suited for this production system. How suitable they are will depend on the system design, as well as the regional characteristics of the market where they will be produced.


2006 ◽  
Vol 49 (2) ◽  
pp. 297-303 ◽  
Author(s):  
Marcelo Ricardo Vicari ◽  
Rubens Pazza ◽  
Roberto Ferreira Artoni ◽  
Vladimir Pavan Margarido ◽  
Luiz Antonio Carlos Bertollo

Hoplias malabaricus (trahyra) is a widespread fish species over the Neotropical region with diversified inter-populational karyotypes (cytotypes), which may correspond to a species complex. Despite the wide distribution in the South American basins, some authors have questioned its natural origin in the Iguaçu river, an important Brazilian river basin which is characterized by several endemic fish species. We have analyzed the karyotype of H. malabaricus from different collection sites of this river, by conventional and banding methods. Our results, in addition to our previous data concerning geographic distribution of the cytotypes, contribute to better understand the origin of H. malabaricus on the Iguaçu river, reinforcing the proposition that it is a natural fish species in this river basin.


Aquaculture ◽  
2014 ◽  
Vol 432 ◽  
pp. 402-417 ◽  
Author(s):  
M.C. Portella ◽  
R.K. Jomori ◽  
N.J. Leitão ◽  
O.C.C. Menossi ◽  
T.M. Freitas ◽  
...  

2011 ◽  
Vol 71 (2) ◽  
pp. 375-380 ◽  
Author(s):  
MC Barros ◽  
EC Fraga ◽  
JLO Birindelli

The Itapecuru is a relatively large river in the northeastern Brazilian state of Maranhão. During several expeditions to this basin, we collected 69 fish species belonging to 65 genera, 29 families and 10 orders. Characiformes and Siluriformes were the orders with the largest number of species and Characidae, Loricariidae, Cichlidae, Auchenipteridae and Pimelodidae were the richest families. About 30% of the fish fauna of the Itapecuru basin is endemic or restricted to northeastern Brazil. Just over a fifth (22%) of the species is also known to occur in the Amazon basin and only a few are more widely distributed in South American.


2018 ◽  
Vol 28 (4) ◽  
pp. 693-714 ◽  
Author(s):  
Jamile Queiroz-Sousa ◽  
Eduardo Meneguzzi Brambilla ◽  
James Raul Garcia-Ayala ◽  
Fabio Alexandre Travassos ◽  
Vanessa Salete Daga ◽  
...  

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