scholarly journals Macrobiotus polypiformis sp. nov., a new tardigrade (Macrobiotidae; hufelandi group) from the Ecuadorian Pacific coast, with remarks on the claw abnormalities in eutardigrades

2017 ◽  
Author(s):  
Milena Roszkowska ◽  
Marta Ostrowska ◽  
Daniel Stec ◽  
Karel Janko ◽  
Łukasz Kaczmarek
Keyword(s):  
New Species ◽  
Taxonomic Status ◽  
28S Rrna ◽  
Microscopy Imaging ◽  
Morphometric Characters ◽  
Mitochondrial Coi ◽  
Taxonomic Description ◽  
As Species ◽  
Species Inquirenda

From a moss sample collected in the Manabí Province in Ecuador, we extracted 96 specimens of a new species of eutardigrade. No eggs were found. In order to obtain eggs, an in vitro culture was prepared. In total, 136 specimens (including ten simplex), one exuvia and 44 eggs (including two with embryos) of the new species were obtained. In addition to the traditional taxonomic description with morphometrics, light and scanning microscopy imaging, we also provide nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) DNA fragments of the new species. Macrobiotus polypiformis sp. nov. belongs to the hufelandi group and is most similar to Ma. paulinae Stec, Smolak, Kaczmarek & Michalczyk, 2015, but differs from it mainly by the lack of dorso-lateral patches of granulation on the cuticle, egg processes with longer and more numerous filaments and in some morphometric characters of both eggs and adults. Moreover, we provide a short discussion on the modifications/abnormalities of the claws in eutardigrades and the possible consequences on the taxonomic status of Mesobiotus armatus (Pilato & Binda, 1996), suggesting its consideration as species inquirenda (with uncertain taxonomic status).

An integrative description of Macrobiotus paulinae sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae: hufelandi group) from Kenya

Zootaxa ◽  
2015 ◽  
Vol 4052 (5) ◽  
pp. 501 ◽  
Author(s):  
DANIEL STEC ◽  
RADOSLAV SMOLAK ◽  
ŁUKASZ KACZMAREK ◽  
ŁUKASZ MICHALCZYK
Keyword(s):  
New Species ◽  
Taxonomic Status ◽  
Integrative Approach ◽  
28S Rrna ◽  
Microscopy Imaging ◽  
Mitochondrial Coi ◽  
Taxonomic Description ◽  
Independent Verification ◽  
Number Of Individuals

In this paper we describe Macrobiotus paulinae, a new species of the hufelandi group from the Kenyan highlands. In addition to the traditional taxonomic description, aided with morphometrics as well as light and scanning microscopy imaging, we also provide nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) DNA fragment of the new species. The sequences allowed not only a more accurate description but also provided an independent verification of the taxonomic status of Ma. paulinae sp. nov. Such integrative approach requires a considerable number of individuals and eggs, which we have partially subsidised by employing an in vitro culture of the new species. Our analyses revealed that Ma. paulinae sp. nov. is most similar to Macrobiotus madegassus Maucci, 1993 and Macrobiotus modestus Pilato & Lisi, 2009, however it differs from these species, as well as from all other known species of the hufelandi group, by the presence of seven paired dorso-lateral patches of cuticular granulation and the presence of chorionic filaments growing out of terminal discs of egg processes. Macrobiotus paulinae sp. nov. is an example of a species with a miniaturised buccal apparatus (i.e. with reduced peribuccal lamellae and oral cavity armature, and stylet supports inserted on the buccal tube more anteriorly than in typical Ma. hufelandi group species), and it therefore resembles two recently described two-macroplacoided Minibiotus species: Mi. acadianus Meyer & Domingue, 2011 and Mi. julianae Meyer, 2012. The re-examination of the type material for these two species confirmed that they are equipped with peribuccal lamellae and therefore we transfer them to the genus Macrobiotus, specifically to the hufelandi group. 

An integrative description of a new tardigrade species Mesobiotus romani sp. nov. (Macrobiotidae: harmsworthi group) from the Ecuadorian Pacific coast

Zootaxa ◽  
2018 ◽  
Vol 4450 (5) ◽  
pp. 550 ◽  
Author(s):  
MILENA ROSZKOWSKA ◽  
DANIEL STEC ◽  
MAGDALENA GAWLAK ◽  
ŁUKASZ KACZMAREK
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Pacific Coast ◽  
28S Rrna ◽  
Microscopy Imaging ◽  
Mitochondrial Coi ◽  
Lichen Sample ◽  
Taxonomic Description ◽  
North Western ◽  
A New Species

In a mixed moss and lichen sample collected in Esmeraldas Province in north-western Ecuador, 20 tardigrades and 11 eggs, belonging to a new species of the genus Mesobiotus, were found. In addition to the traditional taxonomic description with morphometrics, light and scanning microscopy imaging, we also provide nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) DNA fragments of the new species. Based on the egg chorion morphology, Mesobiotus romani sp. nov. is the most similar to: M. binieki, M. coronatus, M. dimentmani, M. patiens, M. perfidus, M. philippinicusi, M. pseudoblocki, M. pseudocoronatus, M. pseudopatiens, M. radiatus, M. rigidus, M. simulans and M. wuzhishanensis, but differs mainly by some specific characters of both egg and adult morphology, and morphometrics. 

Integrative taxonomy identifies Macrobiotus papei, a new tardigrade species of the Macrobiotus hufelandi complex (Eutardigrada: Macrobiotidae) from the Udzungwa Mountains National Park (Tanzania)

Zootaxa ◽  
2018 ◽  
Vol 4446 (2) ◽  
pp. 273 ◽  
Author(s):  
DANIEL STEC ◽  
REINHARDT MØBJERG KRISTENSEN ◽  
ŁUKASZ MICHALCZYK
Keyword(s):  
New Species ◽  
National Park ◽  
Taxonomic Status ◽  
Internal Surface ◽  
Laboratory Culture ◽  
Integrative Approach ◽  
28S Rrna ◽  
Microscopy Imaging ◽  
Morphometric Characters ◽  
Udzungwa Mountains

In this paper we describe Macrobiotus papei, a new species of the Macrobiotus hufelandi complex from the Udzungwa Mountains National Park in Tanzania. Our research included the traditional morphology-based taxonomic analysis, supported by morphometrics, light and scanning electron microscopy imaging, as well as analysis of nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) marker. The molecular sequences provided a more accurate description and independent verification of the taxonomic status of M. papei sp. nov. Such integrative approach requires a considerable number of individuals and eggs, which we obtained by establishing a laboratory culture of the new species. Our analyses revealed that M. papei sp. nov., by having the flexible filaments on the terminal discs of the egg processes, is most similar to Macrobiotus paulinae Stec et al., 2015 from Africa and Macrobiotus polypiformis Roszkowska et al., 2017 from South America. However, the new species can be easily distinguished from both these species by patches of cuticular granulation on the external and internal surface of legs I–III (only external patches present in in M. paulinae and M. polypiformis), two separate lateral transversal ridges with a rounded median tooth between instead of a single thin ventral transverse ridge in the third band of teeth in the oral cavity, smooth terminal discs and filaments on the egg processes (terminal discs and filaments covered by aggregations of small microgranules in M. paulinae and M. polypiformis), and by some morphometric characters of both adults and eggs. 

An integrative description of Pilatobius nuominensis sp. nov. (Tardigrada: Hypsibiidae) from China

Zootaxa ◽  
2021 ◽  
Vol 5026 (1) ◽  
pp. 65-76
Author(s):  
XUE-LING SUN ◽  
JING-YU ZHANG ◽  
NING WANG ◽  
MIN ZHAO ◽  
XUE-GANG LUO
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Taxonomic Status ◽  
First Record ◽  
Nucleotide Sequences ◽  
28S Rrna ◽  
Dna Fragments ◽  
Caudal Region ◽  
Mitochondrial Coi ◽  
Independent Verification

A newly identified tardigrade species from China, Pilatobius nuominensis sp. nov., belongs to the group of species with cuticle of the dorsal and lateral caudal region with evident irregular polygonal sculpture. Nucleotide sequences of two nuclear (18S rRNA, 28S rRNA) and one mitochondrial (COI) DNA fragments of the new species are provided, which allows an independent verification of the taxonomic status of the new species. This is the first record of the genus Pilatobius in the Great Hinggan Mountains.  

Notes on the known species of Trichomyia Haliday of Mexico, with the establishment of a synonymy and the description of a new species (Diptera: Psychodidae)

Zootaxa ◽  
2004 ◽  
Vol 523 (1) ◽  
pp. 1 ◽  
Author(s):  
SERGIO IBÁÑEZ-BERNAL
Keyword(s):  
New Species ◽  
The State ◽  
Female Specimen ◽  
Male And Female ◽  
First Report ◽  
As Species ◽  
Species Inquirenda ◽  
A New Species

Only three species of Trichomyia Haliday have been recorded in Mexico. Trichomyia cirrata Coquillett, 1902, is recognized by its female characteristics, T. fairchildi Vargas and D az-N jera, 1953, was described based only on the wing and must be considered as species inquirenda, and T. maldonadoi (Vargas, 1953), which was described based on one female specimen. Specimens of Trichomyia collected in the states of Campeche, Yucatan, and Oaxaca, Mexico, allowed this female to be associated with the male of T. brevitarsa (Rapp, 1945), thus providing a first report of this species in Mexico. Consequently, T. maldonadoi is proposed as a synonym of T. brevitarsa. Additionally, a new species of Trichomyia from the state of Veracruz, Mexico, is described and illustrated based on the male and female characteristics.

scholarly journals Taxonomical considerations and molecular phylogeny of the closely related genera Bitylenchus, Sauertylenchus and Tylenchorhynchus (Nematoda: Telotylenchinae), with one new and four known species from Iran

2020 ◽  
Vol 94 ◽  
Author(s):  
M. Hosseinvand ◽  
A. Eskandari ◽  
S. Ganjkhanloo ◽  
R. Ghaderi ◽  
P. Castillo ◽  
...  
Keyword(s):  
New Species ◽  
Anterior Part ◽  
Phylogenetic Analyses ◽  
Ribosomal Subunit ◽  
Molecular Data ◽  
28S Rrna ◽  
Natural Habitats ◽  
Morphometric Characters ◽  
Molecular Approaches ◽  
Tail Tip

Abstract During several nematological surveys in cultivated and natural habitats in Khuzestan and Zanjan provinces of Iran, a new species, Bitylenchus parvulus n. sp., two new records for Iran – namely, Tylenchorhynchus agri and Tylenchorhynchus graciliformis – and a population of Bitylenchus parvus and one of Sauertylenchus maximus were recovered and characterized based upon morphological and molecular approaches. The new species is characterized by lip region with five to seven annuli, stylet 17.7 (17.0–18.5) μm long, sub-cylindrical tail narrowing abruptly near terminus giving a bluntly digitate shape to the tail tip, cuticle near anterior part of vulva wrinkled and post-rectal sac occupies whole of tail cavity. The phylogenetic analyses were carried out using molecular data from D2–D3 expansion segments of large ribosomal subunit (28S rRNA) for all studied species and the partial small ribosomal subunit (18S rRNA) for the new species. The representatives of Bitylenchus and Sauertylenchus formed distinct clades from Tylenchorhynchus members, supporting the hypothesis in which Bitylenchus and Sauertylenchus could be considered as valid genera, but rejecting the ‘large-genus’ concept for Tylenchorhynchus. Also, Sauertylenchus ibericus was proposed as a junior synonym of S. maximus based on the results from morphological and phylogenetic analysis. Furthermore, an identification key for all known species included in the three genera Bitylenchus, Tylenchorhynchus and Sauertylenchus is presented herein. The number of transverse annuli on the lip region and presence/absence of post-rectal sac were considered as the main diagnostic characters for classifying the species into seven groups, and other morphological and morphometric characters were subsequently used for distinguishing species in each group.

scholarly journals Integrative description of two new species of Malagasy chirping giant pill-millipedes, genus Sphaeromimus (Diplopoda: Sphaerotheriida: Arthrosphaeridae)

2017 ◽  
Author(s):  
Leif Moritz ◽  
Thomas Wesener
Keyword(s):  
New Species ◽  
Coi Gene ◽  
Micro Ct ◽  
Mitochondrial Coi ◽  
Taxonomic Description ◽  
Mitochondrial Coi Gene ◽  
Two New Species ◽  
Ct Analysis ◽  
Ct Technology ◽  
First Time

The species-rich giant pill-millipedes (Sphaerotheriida) often represent a microendemic component of Madagascar’s mega-invertebrate fauna. Of the chirping genus Sphaeromimus de Saussure & Zehntner, 1902, ten species have been described. Here, we describe two new species of Sphaeromimus integratively, combining light microscopy, scanning electron microscopy, DNA barcoding and micro-CT technology for the first time in a taxonomic description of a giant pill-millipede. S. kalambatritra sp. nov. and S. midongy sp. nov. are the first giant pill-millipedes collected and described from the mountainous rainforests of Kalambatritra and Midongy. Both species show island gigantism compared to their congeners. Our analysis of the mitochondrial COI gene shows that the two species are related to one another with a moderate genetic distance (9.4%), while they are more closely related to an undetermined specimen from the forest of Vevembe (6.3% and 8.4%). They stand in a basal position with S. ivohibe Wesener, 2014 and S. musicus (de Saussure & Zehntner, 1897). The four aforementioned species share a high number of stridulation ribs on the male harp. Our micro-CT analysis provides a look into the head of S. kalambatritra sp. nov. and shows that non-destructive CT methods are a useful tool for studying the inner morphology of giant pill-millipedes.

scholarly journals Integrative Descriptions of Two New Tardigrade Species along with the New Record of Mesobiotus skorackii Kaczmarek et al., 2018 from Canada

Diversity ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 394
Author(s):  
Pushpalata Kayastha ◽  
Milena Roszkowska ◽  
Monika Mioduchowska ◽  
Magdalena Gawlak ◽  
Łukasz Kaczmarek
Keyword(s):  
Large Population ◽  
Original Description ◽  
Molecular Data ◽  
28S Rrna ◽  
Moss Sample ◽  
Morphometric Characters ◽  
Mitochondrial Coi ◽  
Canadian Population ◽  
Coi Sequences ◽  
Morphology And Morphometry

Two new tardigrade species from a moss sample collected in Canada, one representing Macrobiotus hufelandi complex and the second one belonging to the genus Bryodelphax, are described. Integrative analysis was undertaken based on morphological and morphometric data (using both light and scanning electron microscopy (SEM)) combined with multilocus molecular analysis (nuclear sequences, i.e., 18S rRNA, 28S rRNA and ITS-2 as well as mitochondrial COI barcode sequences). Based on COI sequences, Macrobiotus birendrai sp. nov. is most similar to Mac. canaricus (p-distance 17%), whereas Bryodelphax mareki sp. nov. is most similar to Bry. parvulus (p-distance 16%). Both species differ also from their congeners in some morphological and morphometric characters of adults and/or details of egg chorion. Additionally, a large population of Mesobiotus skorackii was found in the sample and this is the first report of this species outside its terra typica in Kirghizia. The original description of this species was prepared based solely on the morphology and morphometry, therefore, here we provide updated data for this species enclosing morphometric and molecular data for the Canadian population.

The superficial ant: a revision of the Neotropical ant-mimicking spider genus Myrmecium Latreille, 1824 (Araneae, Corinnidae, Castianeirinae)

Zootaxa ◽  
2017 ◽  
Vol 4230 (1) ◽  
pp. 1
Author(s):  
DAVID F. CANDIANI ◽  
ALEXANDRE B. BONALDO
Keyword(s):  
New Species ◽  
British Guiana ◽  
Mato Grosso ◽  
Male And Female ◽  
Madre De Dios ◽  
As Species ◽  
Species Inquirenda ◽  
First Time

The ant-mimiking spider genus Myrmecium Latreille, 1824 is revised, including 38 species, all herein diagnosed, described and illustrated. The following synonymies are proposed: Myrmecium aurantiacum Mello-Leitão, 1941 syn. nov. with M. camponotoides Mello-Leitão, 1932; M. gounellei Simon, 1896 syn. nov. and M. obscurum Keyserling, 1891 syn. nov. with M. latreillei Lucas, 1857; M. itatiaiae Mello-Leitão, 1932 syn. nov. and M. vertebratum Walckenaer, 1837 syn. nov. with M. rufum Latreille, 1824. Myrmecium bonaerense Holmberg, 1881 is considered as species inquirenda. Males of M. dacetoniforme, Mello-Leitão, 1932, M. fuscum Dahl, 1907, M. latreillei Lucas, 1857, M. trifasciatum Caporiacco, 1947 and adults of M. viehmeyeri Dahl, 1907 and M. reticulatum Dahl, 1907 are described for the first time. Myrmecium bifasciatum Taczanowski, 1874, M. monacanthum Simon, 1897 and M. rufum Latreille, 1824 are also redescribed and illustrated. The following 28 new species are described, diagnosed and illustrated: M. amphora sp. nov. (female from Chichiriviche, Venezuela); M. bolivari sp. nov. (male and female from Caracas and Sucre, Venezuela and Colombia); M. carajas sp. nov. (male from Pará, Brazil); M. carvalhoi sp. nov. (female from Piauí, Tocantins and Goiás, Brazil); M. catuxy sp. nov. (female from Amazonas, Brazil and Puerto Lopez, Colombia); M. chikish sp. nov. (female from Huánuco, Peru); M. cizauskasi sp. nov. (male and female from Amazonas, Brazil); M. oliveirai sp. nov. (male from Amazonas, Brazil); M. deladanta sp. nov. (male from Sucúmbios, Ecuador); M. diasi sp. nov. (male and female from Amazonas, Brazil); M. erici sp. nov. (female from British Guiana); M. ferro sp. nov. (female from Paraiba, Brazil); M. indicatti sp. nov. (male and female from Pará, Brazil); M. nogueirai sp. nov. (female from Amazonas, Brazil and Madre de Dios, Peru); M. lomanhungae sp. nov. (male and female from Amazonas and Pará, Brazil); M. machetero sp. nov. (female from Beni, Bolivia); M. malleum sp. nov. (male and female from Aragua and Lara, Venezuela and Caldas, Colombia); M. oompaloompa sp. nov. (male and female from Bahia, Brazil and Kurupukari, Guyana); M. otti sp. nov. (male and female from Pará, Amazonas and Mato Grosso, Brazil and Madre de Dios in Peru); M. pakpaka sp. nov. (male and female from Huánuco, Peru); M. raveni sp. nov. (male and female from Amazonas and Pará, Brazil); M. ricettii sp. nov. (male and female from the states of Pará, Alagoas, Sergipe, Bahia, Goiás and Mato Grosso, Brazil and Puerto Lopez, Colombia); M. luepa sp. nov. (male from Bolívar, Venezuela); M. souzai sp. nov. (male from Amazonas, Brazil); M. tanguro sp. nov. (male and female from Rondonia, Mato Grosso, Brazil); M. tikuna sp. nov. (male from Amazonas, Brazil); M. urucu sp. nov. (female from Amazonas, Brazil); M. yamamotoi sp. nov. (male and female from Amapá, Amazonas and Pará, Brazil and Marowijne, Suriname). 

An annotated world catalogue of the millipede order Callipodida (Arthropoda: Diplopoda)

Zootaxa ◽  
2008 ◽  
Vol 1706 (1) ◽  
pp. 1 ◽  
Author(s):  
PAVEL STOEV ◽  
PETRA SIERWALD ◽  
AMBER BILLEY
Keyword(s):  
Taxonomic Status ◽  
Original Description ◽  
Nominal Species ◽  
Index Card ◽  
Species List ◽  
As Species ◽  
Species Inquirenda ◽  
On Line ◽  
First World ◽  
First Descriptions

The paper presents the first world catalogue of the millipede order Callipodida (Diplopoda: Helminthomorpha). The order is currently known to comprise 3 suborders, 7 families, 35 valid (sub-)genera and 133 (sub-)species. Furthermore, 10 nominal species, whose taxonomic status has not been considered in any publications other than the first descriptions, are listed as species inquirenda. The synonymy of Paracortina wangi Stoev, 2004 under Angulifemur unidigitis Zhang, 1997 is formalized here. Given for each (sub-)species are the original description with author, year, pages and figures; complete chronological list of subsequent faunistic or taxonomic references; type material and, if known, current repository; type locality; species range; and sometimes additional remarks on its status or distribution. The relevant taxonomic and faunistic literature on Diplopoda was consulted to complete the data presented here. The species list is based on a species index card catalogue housed in the Museum National d’Histoire Naturelle in Paris (“Fichier iconographiqueBrolemann et successeurs”), with additions from the Zoological Record and various on-line resources. A bibliography containing 286 taxonomic references relevant to the Callipodida is included.

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