An integrative description of Pilatobius nuominensis sp. nov. (Tardigrada: Hypsibiidae) from China

Zootaxa ◽  
2021 ◽  
Vol 5026 (1) ◽  
pp. 65-76
Author(s):  
XUE-LING SUN ◽  
JING-YU ZHANG ◽  
NING WANG ◽  
MIN ZHAO ◽  
XUE-GANG LUO
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Taxonomic Status ◽  
First Record ◽  
Nucleotide Sequences ◽  
28S Rrna ◽  
Dna Fragments ◽  
Caudal Region ◽  
Mitochondrial Coi ◽  
Independent Verification

A newly identified tardigrade species from China, Pilatobius nuominensis sp. nov., belongs to the group of species with cuticle of the dorsal and lateral caudal region with evident irregular polygonal sculpture. Nucleotide sequences of two nuclear (18S rRNA, 28S rRNA) and one mitochondrial (COI) DNA fragments of the new species are provided, which allows an independent verification of the taxonomic status of the new species. This is the first record of the genus Pilatobius in the Great Hinggan Mountains.  

An integrative description of Macrobiotus paulinae sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae: hufelandi group) from Kenya

Zootaxa ◽  
2015 ◽  
Vol 4052 (5) ◽  
pp. 501 ◽  
Author(s):  
DANIEL STEC ◽  
RADOSLAV SMOLAK ◽  
ŁUKASZ KACZMAREK ◽  
ŁUKASZ MICHALCZYK
Keyword(s):  
New Species ◽  
Taxonomic Status ◽  
Integrative Approach ◽  
28S Rrna ◽  
Microscopy Imaging ◽  
Mitochondrial Coi ◽  
Taxonomic Description ◽  
Independent Verification ◽  
Number Of Individuals

In this paper we describe Macrobiotus paulinae, a new species of the hufelandi group from the Kenyan highlands. In addition to the traditional taxonomic description, aided with morphometrics as well as light and scanning microscopy imaging, we also provide nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) DNA fragment of the new species. The sequences allowed not only a more accurate description but also provided an independent verification of the taxonomic status of Ma. paulinae sp. nov. Such integrative approach requires a considerable number of individuals and eggs, which we have partially subsidised by employing an in vitro culture of the new species. Our analyses revealed that Ma. paulinae sp. nov. is most similar to Macrobiotus madegassus Maucci, 1993 and Macrobiotus modestus Pilato & Lisi, 2009, however it differs from these species, as well as from all other known species of the hufelandi group, by the presence of seven paired dorso-lateral patches of cuticular granulation and the presence of chorionic filaments growing out of terminal discs of egg processes. Macrobiotus paulinae sp. nov. is an example of a species with a miniaturised buccal apparatus (i.e. with reduced peribuccal lamellae and oral cavity armature, and stylet supports inserted on the buccal tube more anteriorly than in typical Ma. hufelandi group species), and it therefore resembles two recently described two-macroplacoided Minibiotus species: Mi. acadianus Meyer & Domingue, 2011 and Mi. julianae Meyer, 2012. The re-examination of the type material for these two species confirmed that they are equipped with peribuccal lamellae and therefore we transfer them to the genus Macrobiotus, specifically to the hufelandi group. 

scholarly journals Two new species of the Macrobiotus hufelandi complex (Tardigrada: Eutardigrada: Macrobiotidae) from Australia and India, with notes on their phylogenetic position

2019 ◽  
Author(s):  
Kyle Coughlan ◽  
Daniel Stec
Keyword(s):  
Dna Sequences ◽  
18S Rrna ◽  
Taxonomic Status ◽  
Phylogenetic Position ◽  
28S Rrna ◽  
Future Studies ◽  
Mitochondrial Coi ◽  
Definition Of ◽  
Group Species ◽  
Independent Species

In this paper we describe two new tardigrade species belonging to the Macrobiotus hufelandi complex: Macrobiotus noongaris sp. nov. from Perth, Australia, and Macrobiotus kamilae sp. nov. from Mussoorie, India. Live specimens extracted from moss samples were used to establish laboratory cultures in order to obtain additional animals and eggs needed for their integrative descriptions. These descriptions are based on traditional morphological and morphometric data collected using both light and scanning electron microscopy, which, at the same time, were associated with DNA sequences of four genetic markers, three nuclear (18S rRNA, 28S rRNA and ITS-2) and one mitochondrial (COI). The use of DNA sequences allowed for a more accurate verification of the taxonomic status of M. noongaris sp. nov. and M. kamilae sp. nov as independent species of the hufelandi group. Although they both exhibit typical inverted goblet-shaped processes, they represent a recently discovered clade, which was thought to group species with modified morphology of egg processes. Thus, this contribution expands the definition of the mentioned clade and constitutes another link that will be helpful for future studies on the evolution of the M. hufelandi complex.

scholarly journals Macrobiotus polypiformis sp. nov., a new tardigrade (Macrobiotidae; hufelandi group) from the Ecuadorian Pacific coast, with remarks on the claw abnormalities in eutardigrades

2017 ◽  
Author(s):  
Milena Roszkowska ◽  
Marta Ostrowska ◽  
Daniel Stec ◽  
Karel Janko ◽  
Łukasz Kaczmarek
Keyword(s):  
New Species ◽  
Taxonomic Status ◽  
28S Rrna ◽  
Microscopy Imaging ◽  
Morphometric Characters ◽  
Mitochondrial Coi ◽  
Taxonomic Description ◽  
As Species ◽  
Species Inquirenda

From a moss sample collected in the Manabí Province in Ecuador, we extracted 96 specimens of a new species of eutardigrade. No eggs were found. In order to obtain eggs, an in vitro culture was prepared. In total, 136 specimens (including ten simplex), one exuvia and 44 eggs (including two with embryos) of the new species were obtained. In addition to the traditional taxonomic description with morphometrics, light and scanning microscopy imaging, we also provide nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) DNA fragments of the new species. Macrobiotus polypiformis sp. nov. belongs to the hufelandi group and is most similar to Ma. paulinae Stec, Smolak, Kaczmarek & Michalczyk, 2015, but differs from it mainly by the lack of dorso-lateral patches of granulation on the cuticle, egg processes with longer and more numerous filaments and in some morphometric characters of both eggs and adults. Moreover, we provide a short discussion on the modifications/abnormalities of the claws in eutardigrades and the possible consequences on the taxonomic status of Mesobiotus armatus (Pilato & Binda, 1996), suggesting its consideration as species inquirenda (with uncertain taxonomic status).

An integrative description of a new tardigrade species Mesobiotus romani sp. nov. (Macrobiotidae: harmsworthi group) from the Ecuadorian Pacific coast

Zootaxa ◽  
2018 ◽  
Vol 4450 (5) ◽  
pp. 550 ◽  
Author(s):  
MILENA ROSZKOWSKA ◽  
DANIEL STEC ◽  
MAGDALENA GAWLAK ◽  
ŁUKASZ KACZMAREK
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Pacific Coast ◽  
28S Rrna ◽  
Microscopy Imaging ◽  
Mitochondrial Coi ◽  
Lichen Sample ◽  
Taxonomic Description ◽  
North Western ◽  
A New Species

In a mixed moss and lichen sample collected in Esmeraldas Province in north-western Ecuador, 20 tardigrades and 11 eggs, belonging to a new species of the genus Mesobiotus, were found. In addition to the traditional taxonomic description with morphometrics, light and scanning microscopy imaging, we also provide nucleotide sequences of three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) DNA fragments of the new species. Based on the egg chorion morphology, Mesobiotus romani sp. nov. is the most similar to: M. binieki, M. coronatus, M. dimentmani, M. patiens, M. perfidus, M. philippinicusi, M. pseudoblocki, M. pseudocoronatus, M. pseudopatiens, M. radiatus, M. rigidus, M. simulans and M. wuzhishanensis, but differs mainly by some specific characters of both egg and adult morphology, and morphometrics. 

scholarly journals New species of Acoela from the Mediterranean, the Red Sea, and the South Pacific

Zootaxa ◽  
2011 ◽  
Vol 2867 (1) ◽  
pp. 1 ◽  
Author(s):  
KARIN SARA NILSSON ◽  
ANDREAS WALLBERG ◽  
Ulf JONDELIUS
Keyword(s):  
New Species ◽  
Red Sea ◽  
18S Rrna ◽  
New Caledonia ◽  
South Pacific ◽  
28S Rrna ◽  
The South ◽  
Mitochondrial Coi ◽  
Molecular Phylogenetic ◽  
The Mediterranean

Eight new species of Acoela are reported including the first Acoela recorded from New Caledonia and the first acoel described from volcanic carbon dioxide vents. Pharyngia furva gen. et sp.nov. (Isodiametridae) and Philactinoposthia brevis sp.nov. (Actinoposthiidae) from the Red Sea; Childia curinii sp.nov. (Childiidae), Philactinoposthia ischiae sp.nov. and Philactinoposthia multipunctata sp.nov. from the Mediterranean; Philactinoposthia novaecaledoniae sp.nov., Childia aculifera sp.nov. and Solenofilomorpha justinei sp.nov. (Solenofilomorphidae) from the South Pacific are described. Nucleotide sequences for the nuclear ribosomal 18S rRNA, 28S rRNA and the mitochondrial COI genes were determined for the new species and used in a Bayesian molecular phylogenetic analysis.

scholarly journals Integrative description of Mesobiotus anastasiae sp. nov. (Eutardigrada, Macrobiotoidea) and first record of Lobohalacarus (Chelicerata, Trombidiformes) from the Republic of South Africa

2020 ◽  
Vol 726 ◽  
Author(s):  
Denis V. Tumanov
Keyword(s):  
South Africa ◽  
New Species ◽  
18S Rrna ◽  
First Record ◽  
Mite Species ◽  
28S Rrna ◽  
Unique Combination ◽  
Morphological Approach ◽  
The Republic ◽  
A New Species

A new species of the genus Mesobiotus is described from the Republic of South Africa using a traditional morphological approach (light and scanning electron microscopy) combined with molecular analysis (18S rRNA, 28S rRNA, ITS-2 and COI markers). Mesobiotus anastasiae sp. nov. differs from all known Mesobiotus species by having a unique combination of characters of the adult animals and the eggs. Adults of the new species have an oral cavity armature without elongate teeth in the second band, while the processes of the egg chorion have a basal collar and distinct rows of large pores. An updated key to the species of the genus Mesobiotus, including 66 of 70 currently described species, is given. An aquatic mite species from the Lobohalacarus weberi complex (freshwater Halacaridae) co-occurs with M. anastasiae sp. nov., suggesting that the newly described tardigrade inhabits constantly wet moss cushion habitats.

Whale falls, multiple colonisations of the deep, and the phylogeny of Hesionidae (Annelida)

2015 ◽  
Vol 29 (2) ◽  
pp. 105 ◽  
Author(s):  
Mindi Summers ◽  
Fredrik Pleijel ◽  
Greg W. Rouse
Keyword(s):  
New Species ◽  
Maximum Likelihood ◽  
16S Rrna ◽  
Deep Sea ◽  
Phylogenetic Relationships ◽  
18S Rrna ◽  
Shallow Waters ◽  
28S Rrna ◽  
Mitochondrial Cytochrome ◽  
New Members

Phylogenetic relationships within Hesionidae Grube, 1850 are assessed via maximum parsimony and maximum likelihood analyses of mitochondrial (cytochrome c oxidase subunit I and 16S rRNA) and nuclear (18S rRNA, and 28S rRNA) data. The analyses are based on 42 hesionid species; six of these being new species that are described here. The new species, all from deep (>200 m depth) benthic environments (including whale falls) in the eastern Pacific, are Gyptis shannonae, sp. nov., Neogyptis julii, sp. nov., Sirsoe sirikos, sp. nov., Vrijenhoekia ketea, sp. nov., Vrijenhoekia falenothiras, sp. nov., and Vrijenhoekia ahabi, sp. nov. The molecular divergence among the new members of Vrijenhoekia is pronounced enough to consider them cryptic species, even though we cannot distinguish among them morphologically. Our results also showed that the subfamily Hesioninae Grube, 1850, as traditionally delineated, was paraphyletic. We thus restrict Hesioninae to include only Hesionini Grube, 1850 and refer the remaining members to Psamathinae Pleijel, 1998. The present study increases the number of hesionid species associated with whale falls from one to six and markedly increases the number of described deep-sea hesionid taxa. There appear to have been multiple colonisations of the deep sea from shallow waters by hesionids, though further sampling is warranted.

Morphological and molecular characterisation of Helicotylenchus ciceri n. sp. and H. scoticus Boag & Jairajpuri, 1985 (Nematoda: Hoplolaimidae) from Iran

Nematology ◽  
2020 ◽  
Vol 22 (6) ◽  
pp. 611-626
Author(s):  
Fariba Mohammadi Zameleh ◽  
Akbar Karegar ◽  
Reza Ghaderi ◽  
Abbas Mokaram Hesar
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
18S Rrna ◽  
Phylogenetic Analyses ◽  
Rrna Genes ◽  
28S Rrna ◽  
Molecular Characterisation ◽  
Closely Related Species ◽  
Partial 18S ◽  
Molecular Characters

Summary Helicotylenchus ciceri n. sp. and H. scoticus are described and illustrated based on morphological, morphometric and molecular characters. The new species is characterised by a conical and truncated lip region with five or six distinct annuli, stylet 32-37 μm long with anteriorly concave knobs, secretory-excretory pore posterior to the pharyngo-intestinal valve, dorsally convex-conoid tail with a terminal projection, phasmids 14 (7-20) annuli anterior to the level of anus, empty spermatheca and absence of males. Intraspecific variation of 16 populations of H. scoticus, collected from chickpea and lentil fields in Kermanshah province, western Iran, is discussed. The results of the phylogenetic analyses based on the sequences of the partial 18S rRNA, D2-D3 expansion segments of 28S rRNA and ITS rRNA genes are provided for the studied species, confirming their differences from each other and determining the position of them and their relationships with closely related species.

Determining the position of Diomocoris, Micromimetus and Taylorilygus in the Lygus-complex based on molecular data and first records of Diomocoris and Micromimetus from Australia, including four new species (Insecta : Hemiptera : Miridae : Mirinae)

2021 ◽  
Author(s):  
Anna A. Namyatova ◽  
Michael D. Schwartz ◽  
Gerasimos Cassis
Keyword(s):  
New Species ◽  
16S Rrna ◽  
Morphological Study ◽  
18S Rrna ◽  
Initial Step ◽  
Sister Group ◽  
Molecular Data ◽  
28S Rrna ◽  
Group Relationships ◽  
First Time

The Lygus-complex is one of the most taxonomically challenging groups of Miridae (Heteroptera), and its Australian fauna is poorly studied. Here we examine the Australian taxa of the Lygus-complex using morphological and molecular methods. After a detailed morphological study of the material collected throughout Australia, Taylorilygus nebulosus is transferred to Diomocoris, with the genus recorded for the first time in this country. Taylorilygus apicalis, also widely distributed in Australia, is redescribed on the basis of Australian material. The genus Micromimetus is recorded for the first time in Australia, with M. celiae, sp. nov., M. hannahae, sp. nov., M. nikolai, sp. nov. and M. shofneri, sp. nov. described as new to science. Micromimetus pictipes is redescribed and its distributional range is increased. The monophyly of the Lygus-complex and relationships within this group were tested using cytochrome c oxidase subunit I (COI), 16S rRNA, 18S rRNA and 28S rRNA markers. The Lygus-complex has been found to be non-monophyletic. Phylogeny confirmed the monophyly of Micromimetus, and it has shown that Taylorilygus apicalis is closer to Micromimetus species than to Diomocoris nebulosus. This study is the initial step in understanding the Lygus-complex phylogeny; analyses with more taxa, more genes and morphology are needed to reveal the interrelationships within this group, and sister-group relationships of Australian taxa. http://zoobank.org/urn:lsid:zoobank.org:pub:7393D96B-2BBA-438D-A134-D372EFE7FB9E

Periconia citlaltepetlensis sp. nov. (Periconiaceae, Pleosporales): a psychrotolerant fungus from high elevation volcanic glacier (Mexico)

Phytotaxa ◽  
2020 ◽  
Vol 459 (3) ◽  
pp. 235-247
Author(s):  
ROSA P. CALVILLO-MEDINA ◽  
AURORA COBOS-VILLAGRÁN ◽  
TANIA RAYMUNDO
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
First Record ◽  
High Elevation ◽  
Nucleotide Sequences ◽  
Species Level ◽  
Rdna Analysis

Periconia citlaltepetlensis sp. nov. is the first record of a fungus identified to species level from high elevation volcanic glacier in Mexico. It is a novel dothideomycete species supported by morphological, cultural characters and phylogenetic analysis (nucleotide sequences data rDNA analysis). The new species is characterized by conidiophores often with 2–4 enteroblastic, percurrent proliferations, large, globose, golden brown or reddish brown conidia that are borne singly or in basipetal chains, verrucose to irregular lobate crests, born singly or in basipetal chains of two conidia; acropetally proliferating; conidiogenesis holoblastic. The morphology of P. citlaltepetlensis is compared to other Periconia species and a detailed description and morphological illustrations are provided.

Sign in / Sign up

Export Citation Format

Share Document