Multiplex Immunoassay Analysis of Cytokines in Idiopathic Inflammatory Myopathy

2008 ◽  
Vol 132 (2) ◽  
pp. 232-238 ◽  
Author(s):  
Geoffrey S. Baird ◽  
Thomas J. Montine
Keyword(s):  
Adhesion Molecule ◽  
Muscle Protein ◽  
Inflammatory Myopathy ◽  
Idiopathic Inflammatory Myopathy ◽  
Monocyte Chemoattractant Protein 1 ◽  
Multiplex Immunoassay ◽  
Markers Of Inflammation ◽  
Intracellular Adhesion Molecule ◽  
Histopathologic Analysis ◽  
Total Lysate

Abstract Context.—Idiopathic inflammatory myopathies (IIMs), including dermatomyositis, polymyositis, and inclusion-body myositis, can be difficult to diagnose. Objective.—To determine if a multiplex immunoassay for markers of inflammation in muscle homogenates correlates with a diagnosis of IIM. Design.—Frozen archived muscle biopsy specimens from 30 patients with IIM and 34 patients without IIM were homogenized and analyzed for cytokine content with a multiplex microbead-based immunoassay system. Analyte concentrations were normalized to total lysate protein concentration prior to comparison. Results.—Two cytokines, interleukin 1ra and monocyte chemoattractant protein 1, and 1 soluble adhesion molecule, intracellular adhesion molecule 1, were found at significantly greater concentrations in muscle samples from patients with IIM. Intracellular adhesion molecule 1 levels alone were 83% sensitive and 91% specific for IIM at a cutoff of 1240 pg/mg muscle protein. Conclusions.—Immunoassays for selected inflammatory markers can serve in conjunction with histopathologic analysis as sensitive and specific tools for the diagnosis of IIM.

scholarly journals Effects of Acute Fructose Loading on Markers of Inflammation—A Pilot Study

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 3110
Author(s):  
Camilla Olofsson ◽  
Monica Eriksson ◽  
Ann-Christin Bragfors Helin ◽  
Björn Anderstam ◽  
Nicola Orsini ◽  
...  
Keyword(s):  
Adhesion Molecule ◽  
Inflammatory Markers ◽  
Intercellular Adhesion Molecule ◽  
Low Grade ◽  
Intercellular Adhesion Molecule 1 ◽  
Monocyte Chemoattractant Protein 1 ◽  
Postprandial State ◽  
Markers Of Inflammation ◽  
Bacterial Lipopolysaccharides ◽  
Cell Adhesion Molecule 1

Inflammation plays a role in development of diabetic complications. The postprandial state has been linked to chronic low grade inflammation. We therefore aimed to investigate the acute effects of fructose loading, with and without a pizza, on metabolic and inflammatory markers in patients with type 2 diabetes (T2D) (n = 7) and in healthy subjects (HS) (n = 6), age 47–76 years. Drinks consumed were blueberry drink (18 g fructose), Coca-Cola (17.5 g fructose), and fructose drink (35 g fructose). The levels of glucose, insulin, insulin-like growth factor binding protein-1 (IGFBP-1) and inflammatory markers: Interleukin-6 (IL-6), Monocyte chemoattractant protein-1 (MCP-1), Interleukin-18 (IL-18), Intercellular Adhesion Molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), and bacterial lipopolysaccharides (LPS) were analyzed in blood. The postprandial responses were assessed using Wilcoxon’s matched-pairs test, Friedman’s ANOVA and Mann–Whitney U test. There was no difference in baseline levels of inflammatory markers between the groups. In T2D, MCP-1 decreased following blueberry drink and Coca-Cola (p = 0.02), Coca-Cola + pizza and fructose + pizza (p = 0.03). In HS, IL-6 increased following blueberry + pizza and fructose + pizza (p = 0.03), there was a decrease in MCP-1 following blueberry drink and Coca-Cola (p = 0.03), and in ICAM-1 following blueberry + pizza (p = 0.03). These results may indicate a role for MCP-1 as a link between postprandial state and diabetes complications, however further mechanistic studies on larger population of patients with T2D are needed for confirmation of these results.

Combined modulation of the mesangial machinery for monocyte recruitment by inhibition of NF-κB

2001 ◽  
Vol 281 (6) ◽  
pp. C1881-C1888 ◽  
Author(s):  
Alma Zernecke ◽  
Kim S. C. Weber ◽  
Christian Weber
Keyword(s):  
Adhesion Molecule ◽  
Mesangial Cells ◽  
Nuclear Factor Κb ◽  
Pharmacological Intervention ◽  
Monocyte Chemoattractant Protein 1 ◽  
Intracellular Adhesion Molecule ◽  
Tnf Α ◽  
Monocyte Chemotaxis ◽  
Or Gene ◽  
Factor Α

The activation of nuclear factor-κB (NF-κB) is required for the induction of many of the adhesion molecules and chemokines involved in the inflammatory leukocyte recruitment to the kidney. Here we studied the effects of NF-κB inhibition on the machinery crucial for monocyte infiltration of the glomerulus during inflammation. In mesangial cells (MC), the protease inhibitors MG-132 and N-α-tosyl-l-lysine chloromethyl ketone or adenoviral overexpression of IκB-α prevented the complete IκB-α degradation following tumor necrosis factor-α (TNF-α) stimulation. This resulted in a marked inhibition of TNF-α-induced expression of mRNA and protein for the immunoglobulin molecules intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 and the chemokines growth-related oncogene-α, monocyte chemoattractant protein-1, interleukin-8, or fractalkine in MC. Finally, the inhibition of IκB-α degradation or IκB-α overexpression suppressed the chemokine-induced transendothelial monocyte chemotaxis toward MC and the chemokine-triggered firm adhesion of monocytic cells to MC. The inhibition of NF-κB by pharmacological intervention or gene transfer may present a multimodal approach to control the machinery propagating inflammatory recruitment of monocytes during glomerular disease.

scholarly journals T1 mapping-derived signature of myocardial involvement in idiopathic inflammatory myopathy compared to acute viral myocarditis: a texture-based analysis

2021 ◽  
Vol 22 (Supplement_1) ◽  
Author(s):  
E Bollache ◽  
AT Huber ◽  
J Lamy ◽  
E Afari ◽  
TM Bacoyannis ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Texture Analysis ◽  
T1 Mapping ◽  
Inflammatory Myopathy ◽  
Viral Myocarditis ◽  
Texture Features ◽  
Idiopathic Inflammatory Myopathy ◽  
Post Contrast ◽  
Myocardial T1 ◽  
Myocardial Involvement

Abstract Funding Acknowledgements Type of funding sources: None. Background. Recent studies revealed the ability of MRI T1 mapping to characterize myocardial involvement in both idiopathic inflammatory myopathy (IIM) and acute viral myocarditis (AVM), as compared to healthy controls. However, neither myocardial T1 nor T2 maps were able to discriminate between IIM and AVM patients, when considering conventional myocardial mean values and derived indices such as lambda and extracellular volume. Purpose. To investigate the ability of T1 mapping-derived texture analysis to differentiate IIM from AVM. Methods. Forty patients, 20 with IIM (51 ± 17 years, 9 men) and 20 with AVM (34 ± 13 years, 16 men) underwent 1.5T MRI T1 mapping using a modified Look-Locker inversion-recovery sequence before and 15 minutes after injection of a gadolinium contrast agent. After manual delineation of endocardial and epicardial borders and co-registration of all inversion time images, native and post-contrast T1 maps were estimated. Myocardial texture analysis was performed on native T1 maps. Textural features such as: autocorrelation, contrast, dissimilarity, energy and sum entropy were used to build a least squares-based linear regression model. Finally, receiver operating characteristic (ROC) analysis was used to investigate the ability of such texture features score to classify IIM vs. AVM patients, compared to the performance of mean myocardial T1. A Wilcoxon rank-sum test was also used to test difference significance between groups. Results. Both native and post-contrast mean myocardial T1 values were comparable between IIM (native: 1022 ± 43 ms; post-contrast: 319 ± 44 ms) and AVM (1056 ± 59 ms, p = 0.07; 318 ± 35 ms, p = 0.90, respectively) groups. Results of ROC analyses are provided in the Table, indicating that a better discrimination between IIM and AVM patients was obtained when using texture features, with higher AUC and accuracy than mean T1 values (Figure). Conclusion. Texture analysis derived from MRI T1 maps without contrast agent injection was able to discriminate between IIM and AVM with higher accuracy, sensitivity and specificity than conventional T1 indices. Such analysis could provide a useful myocardial signature to help diagnose and manage cardiac alterations associated with IIM in patients presenting with myocarditis and primarily suspected of AVM. Table Area under curve (AUC) Accuracy Sensitivity Specificity Native T1 0.67 0.70 0.65 0.75 Post-contrast T1 0.49 0.60 0.25 0.95 Texture features score 0.85 0.82 0.90 0.75 ROC analyses for classification between IIM and AVM patients Abstract Figure

Role of integrins and intracellular adhesion molecule-1 in lung injury after intestinal ischemia-reperfusion

2002 ◽  
Vol 183 (1) ◽  
pp. 70-74 ◽  
Author(s):  
M.Ayhan Kuzu ◽  
Cüneyt Köksoy ◽  
Işınsu Kuzu ◽  
Ismet Gürhan ◽  
Hakan Ergün ◽  
...  
Keyword(s):  
Lung Injury ◽  
Adhesion Molecule ◽  
Intestinal Ischemia ◽  
Ischemia Reperfusion ◽  
Intracellular Adhesion Molecule ◽  
Intestinal Ischemia Reperfusion
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