The Utility of the BIOMED-2 Primers in the Detection of 2 Clonal, B-Lymphoproliferative Disorders Simultaneously Involving the Same Site

2013 ◽  
Vol 137 (11) ◽  
pp. 1654-1659 ◽  
Author(s):  
Brenda Ly ◽  
Claudiu V. Cotta
Keyword(s):  
Monoclonal Gammopathy ◽  
Tissue Sample ◽  
Lymphoproliferative Disorders ◽  
Molecular Tests ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Primer Sets ◽  
Formalin Fixed ◽  
Undetermined Significance ◽  
Abnormal Results

Context.—Molecular tests for clonality performed on atypical lymphoid lesions may yield abnormal results because of the coexistence of monoclonal B lymphocytosis or monoclonal gammopathy of undetermined significance in the sample investigated. Objective.—To investigate the ability of the BIOMED-2 sets of primers to identify 2 clonal populations in the same formalin-fixed, paraffin-embedded tissue sample. Design.—Ten cases with 2 B-lymphoproliferative disorders at the same site were analyzed using 5 BIOMED-2 primer sets (IGH FR1, FR2, FR3, IGK VJ, and IGK VKde). Results.—All 10 cases (100%) showed at least 1 clone; 8 of 10 cases (80%) had 2 clones with at least 1 primer set, and the 2 clones were shown by 4 or 5 primer sets in none of the cases (0%), by 3 sets in 1 of 10 cases (10%), by 2 sets in 4 of 10 cases (40%), and by 1 set in 3 of 10 cases (30%). The most effective set was IGH FR2, detecting 4 of 10 biclonal cases (40%). The IGK VJ and IGK VKde each showed 2 clones in 3 of 10 cases (30% each). The least effective sets were IGH FR1 and FR3, with 2 of 10 cases (20%) each, with IGH FR1 being the least useful. Conclusions.—The BIOMED-2 primers are effective in the detection of 2 clonal populations in the same sample.

scholarly journals The Dutch National TissueArchive Portal enables efficient, consistent, and transparent procurement of diagnostic tissue samples for scientific use

2021 ◽  
Author(s):  
Robin Verjans ◽  
Annette H. Bruggink ◽  
Robby Kibbelaar ◽  
Jos Bart ◽  
Aletta Debernardi ◽  
...  
Keyword(s):  
The Netherlands ◽  
Tissue Sample ◽  
Ffpe Tissue ◽  
Tissue Samples ◽  
Internet Portal ◽  
Formalin Fixed Paraffin ◽  
Practical Support ◽  
Formalin Fixed Paraffin Embedded ◽  
The Rich ◽  
Formalin Fixed

AbstractBiobanks play a crucial role in enabling biomedical research by facilitating scientific use of valuable human biomaterials. The PALGA foundation—a nationwide network and registry of histo- and cytopathology in the Netherlands—was established to promote the provision of data within and between pathology departments, and to make the resulting knowledge available for healthcare. Apart from the pathology data, we aimed to utilize PALGA’s nationwide network to find and access the rich wealth of Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples for scientific use.  We implemented the Dutch National TissueArchive Portal (DNTP) to utilize PALGA’s nationwide network for requesting FFPE tissue samples. The DNTP consists of (1) a centrally organized internet portal to improve the assessing, processing, harmonization, and monitoring of the procurement process, while (2) dedicated HUB-employees provide practical support at peripheral pathology departments. Since incorporation of the DNTP, both the number of filed requests for FFPE tissue samples and the amount of HUB-mediated support increased 55 and 29% respectively. In line, the sample procurement duration time decreased significantly (− 47%). These findings indicate that implementation of the DNTP improved the frequency, efficiency, and transparency of FFPE tissue sample procurement for research in the Netherlands. To conclude, the need for biological resources is growing persistently to enable precision medicine. Here, we access PALGA’s national, pathology network by implementation of the DNTP to allow for efficient, consistent, and transparent exchange of FFPE tissue samples for research across the Netherlands.

scholarly journals Retrospective study of central nervous system lesions and association with Parelaphostrongylus species by histology and specific nested polymerase chain reaction in domestic camelids and wild ungulates

2014 ◽  
Vol 26 (6) ◽  
pp. 748-754 ◽  
Author(s):  
Carrie L. Dobey ◽  
Caroline Grunenwald ◽  
Shelley J. Newman ◽  
Lisa Muller ◽  
Richard W. Gerhold
Keyword(s):  
Polymerase Chain Reaction ◽  
Cervus Elaphus ◽  
Plasma Cells ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Molecular Tests ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Polymerase Chain ◽  
Formalin Fixed

Formalin-fixed, paraffin-embedded tissues from elk ( Cervus elaphus), goats, and camelids with case histories and lesions suggestive of Parelaphostrongylus tenuis were examined by histology to characterize lesions that could aid in definitively diagnosing P. tenuis infection. Additionally, sections of paraffin-embedded tissue were used in a nested polymerase chain reaction (nPCR) using Parelaphostrongylus-specific primers to determine how PCR results corresponded with histological findings. Histological changes in brain and spinal cord consisted of linear tracks of hemorrhage; tracks or perivascular accumulations of hemosiderin-laden macrophages; acute foci of axonal degeneration and/or linear glial scars; and perivascular, parenchymal, or meningeal accumulations of eosinophils and/or lymphocytes and plasma cells. Of the 43 samples with histologic lesions consistent with neural larval migrans, 19 were PCR positive; however, only 8 were confirmed Parelaphostrongylus by DNA sequencing. Additionally, 1 goat was identified with a protostrongylid that had a 97% identity to both Parelaphostrongylus odocoilei and a protostrongylid nematode from pampas deer ( Ozotoceros bezoarticus celer) from Argentina. None of the histologic lesions individually or in combination correlated statistically to positive molecular tests for the nematode. The results indicate that it is possible to extract Parelaphostrongylus DNA from formalin-fixed, paraffin-embedded tissue, but extended fixation presumably can cause DNA crosslinking. Nested PCR provides another diagnostic tool to identify the cause of neurologic disease in camelids and elk with histologic lesions consistent with neural larval migrans. Furthermore, potential novel protostrongylid DNA was detected from a goat with lesions consistent with P. tenuis infection, suggesting that other neurotropic Parelaphostrongylus species may occur locally.

scholarly journals CellBlockistry: Chemistry and art of cell-block making – A detailed review of various historical options with recent advances

CytoJournal ◽  
2019 ◽  
Vol 16 ◽  
pp. 12 ◽  
Author(s):  
Vinod B Shidham
Keyword(s):  
Ffpe Tissue ◽  
Cell Block ◽  
Molecular Tests ◽  
Recent Advances ◽  
Pattern Approach ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Technical Advances ◽  
Ancillary Studies ◽  
Formalin Fixed

Cell-blocks are paraffin-embedded versions of cytology specimens comparable to the formalin-fixed paraffin-embedded (FFPE) tissue from surgical pathology specimens. They allow various elective ancillary studies on a variety of specimens with enhanced cytopathologic interpretation, including opportunity to perform molecular tests. However, different dictionaries and internet search engines primarily project “cellblock” and “cell block” definition in relation to prisons. Most of the top searches lead to information related to “prison cells” followed by a few cytopathology-related searches. Due to this in the current review, it is recommended that the word for cytopathology purposes should be hyphenated and spelled as “cell-block.” Cell-blocks have been increasingly indicated on most cytology specimens. Its role is growing further with the ongoing addition of new immunohistochemistry (IHC) markers with technical advances including multicolor IHC and the SCIP (subtractive coordinate immunoreactivity pattern) approach. In addition, it is an important source of tissue for many ancillary studies even as archived material retrospectively at later stage of management if the cell-blocks are improved qualitatively and quantitatively. Because of this, the significance of cell-block is critical with the increasing number of molecular markers standardized predominantly on FFPE tissue. As compared to core biopsies, high-quality cell-blocks prepared with enhanced methodologies predominantly contain concentrated diagnostic tumor cells required for the molecular tests without significant stromal contamination. This review introduces the terminology of CellBlockistry as the science of studying chemistry and the art of achieving quantitatively and qualitatively improved cell-blocks from different types of specimens. The review addresses the cell-block making process as “cell-blocking” and discusses different historical limitations with emphasis on recent advances.

scholarly journals Tissue Digestion for Ferruginous Bodies for Light Microscopy

1998 ◽  
Vol 6 (1) ◽  
pp. 8-11
Author(s):  
Margaret Gondo
Keyword(s):  
Tissue Sample ◽  
Lung Parenchyma ◽  
Asbestos Fibers ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
The Individual ◽  
Ferruginous Bodies ◽  
Formalin Fixed ◽  
Tissue Digestion

Asbestos fibers are commonly called ferruginous bodies due to the iron content of the fibers. After they are inhaled, the asbestos fibers become coated with glycoprotein and hemosiderin, deposited by macrophages.This procedure may be used on either formalin fixed, paraffin embedded blocks or formalin fixed wet tissue. Because this procedure will result in complete destruction of the tissue sample used, it is very important to obtain written approval documenting this fact from the individual requesting this procedure, if the procedure is being done for someone else. The tissue should be from the lung parenchyma. Also, it is a good idea to use sterile, disposable tubes throughout this procedure to limit the possibility of contamination.

scholarly journals Identification of canine papillomavirus by PCR in Greyhound dogs

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2744 ◽  
Author(s):  
Eman A. Anis ◽  
Linda A. Frank ◽  
Raquel Francisco ◽  
Stephen A. Kania
Keyword(s):  
Dna Sequences ◽  
Sequence Similarity ◽  
The Other ◽  
Total Nucleic Acid ◽  
Inflammatory Dermatoses ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Primer Sets ◽  
Unrelated Adult ◽  
Formalin Fixed

Background Corns are hard protuberances that occur on the digital footpads of Greyhound dogs. The cause of these lesions is unknown and there is little information about them in the veterinary literature. We received anecdotal examples of dog to dog spread of corns suggesting an infectious cause. The aim of this study was to determine if papillomavirus (PV) is associated with Greyhound corns. Methods We examined four corns from two unrelated adult Greyhound dogs that resided in Florida and Washington, respectively, for PV by PCR. The samples were obtained by owner coring of two lesions from one dog and laser removal of two lesions from the other dog. Total nucleic acid was extracted and DNA was amplified using two PCR primer sets that have been shown to amplify a broad range of PVs from humans and animals: FAP59/ FAP64 and MY11/ MY09. The DNA sequences were compared with all sequences in GenBank. Formalin-fixed, paraffin-embedded tissue from the footpads of four dogs with other inflammatory dermatoses were also examined. Results PV DNA was amplified from all four corn lesions, while no PV DNA was amplified from other tissues. Comparison of the 444-bp sequences amplified by the MY11/ MY09 primers identified two different PVs. One showed 96% nucleotide sequence similarity with the L1 gene of canine PV type 12. The other showed 78% similarity to canine PV type 16 and, therefore, represents a novel PV. In one of the corns, infection by two of the identified PVs was found. Discussion These results suggest PV infection could be involved in the pathogenesis of corns in Greyhound dogs.

scholarly journals Identification of canine papillomavirus by PCR in Greyhound dogs

2016 ◽  
Author(s):  
Eman A Anis ◽  
Linda A Frank ◽  
Raquel Francisco ◽  
Stephen A Kania
Keyword(s):  
Nucleotide Sequence ◽  
Dna Sequences ◽  
The Other ◽  
Total Nucleic Acid ◽  
Inflammatory Dermatoses ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Primer Sets ◽  
Unrelated Adult ◽  
Formalin Fixed

Background – Corns are hard protuberances that occur on the digital footpads of Greyhound dogs. The cause of these lesions is unknown and there is little information about them in the veterinary literature. We received anecdotal examples of dog to dog spread of corns suggesting an infectious cause. The aim of this study was to determine if papillomavirus (PV) is associated with Greyhound corns. Methods – We examined four corns from two unrelated adult Greyhound dogs that resided in Florida and Washington, respectively, for PV by PCR. The samples were obtained by owner coring of two lesions from one dog and laser removal of two lesions from the other dog. Total nucleic acid was extracted and DNA was amplified using two PCR primer sets that have been shown to amplify a broad range of PVs from humans and animals: FAP59/ FAP64 and MY11/ MY09. The DNA sequences were compared with all sequences in GenBank. Formalin-fixed, paraffin-embedded tissue from the footpads of four dogs with other inflammatory dermatoses were also examined. Results – PV DNA was amplified from all four corn lesions, while no PV DNA was amplified from other tissues. Comparison of the 300-400-bp sequences amplified by the MY11/ MY09 primers identified two different PVs. One showed 96% nucleotide sequence homology with the L1 gene of canine PV type 12. The other showed 78% homology to canine PV type 16, and, therefore, represents a novel PV. In one of the corns, infection by two of the identified PVs was found. Discussion – These results suggest PV infection could be involved in the pathogenesis of corns in Greyhound dogs.

scholarly journals Identification of canine papillomavirus by PCR in Greyhound dogs

2016 ◽  
Author(s):  
Eman A Anis ◽  
Linda A Frank ◽  
Raquel Francisco ◽  
Stephen A Kania
Keyword(s):  
Nucleotide Sequence ◽  
Dna Sequences ◽  
The Other ◽  
Total Nucleic Acid ◽  
Inflammatory Dermatoses ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Primer Sets ◽  
Unrelated Adult ◽  
Formalin Fixed

Background – Corns are hard protuberances that occur on the digital footpads of Greyhound dogs. The cause of these lesions is unknown and there is little information about them in the veterinary literature. We received anecdotal examples of dog to dog spread of corns suggesting an infectious cause. The aim of this study was to determine if papillomavirus (PV) is associated with Greyhound corns. Methods – We examined four corns from two unrelated adult Greyhound dogs that resided in Florida and Washington, respectively, for PV by PCR. The samples were obtained by owner coring of two lesions from one dog and laser removal of two lesions from the other dog. Total nucleic acid was extracted and DNA was amplified using two PCR primer sets that have been shown to amplify a broad range of PVs from humans and animals: FAP59/ FAP64 and MY11/ MY09. The DNA sequences were compared with all sequences in GenBank. Formalin-fixed, paraffin-embedded tissue from the footpads of four dogs with other inflammatory dermatoses were also examined. Results – PV DNA was amplified from all four corn lesions, while no PV DNA was amplified from other tissues. Comparison of the 300-400-bp sequences amplified by the MY11/ MY09 primers identified two different PVs. One showed 96% nucleotide sequence homology with the L1 gene of canine PV type 12. The other showed 78% homology to canine PV type 16, and, therefore, represents a novel PV. In one of the corns, infection by two of the identified PVs was found. Discussion – These results suggest PV infection could be involved in the pathogenesis of corns in Greyhound dogs.

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