Identification of canine papillomavirus by PCR in Greyhound dogs

Primer Sets ◽

Unrelated Adult ◽

Background – Corns are hard protuberances that occur on the digital footpads of Greyhound dogs. The cause of these lesions is unknown and there is little information about them in the veterinary literature. We received anecdotal examples of dog to dog spread of corns suggesting an infectious cause. The aim of this study was to determine if papillomavirus (PV) is associated with Greyhound corns. Methods – We examined four corns from two unrelated adult Greyhound dogs that resided in Florida and Washington, respectively, for PV by PCR. The samples were obtained by owner coring of two lesions from one dog and laser removal of two lesions from the other dog. Total nucleic acid was extracted and DNA was amplified using two PCR primer sets that have been shown to amplify a broad range of PVs from humans and animals: FAP59/ FAP64 and MY11/ MY09. The DNA sequences were compared with all sequences in GenBank. Formalin-fixed, paraffin-embedded tissue from the footpads of four dogs with other inflammatory dermatoses were also examined. Results – PV DNA was amplified from all four corn lesions, while no PV DNA was amplified from other tissues. Comparison of the 300-400-bp sequences amplified by the MY11/ MY09 primers identified two different PVs. One showed 96% nucleotide sequence homology with the L1 gene of canine PV type 12. The other showed 78% homology to canine PV type 16, and, therefore, represents a novel PV. In one of the corns, infection by two of the identified PVs was found. Discussion – These results suggest PV infection could be involved in the pathogenesis of corns in Greyhound dogs.

Identification of canine papillomavirus by PCR in Greyhound dogs

10.7287/peerj.preprints.2290v1 ◽

2016 ◽

Author(s):

Eman A Anis ◽

Linda A Frank ◽

Raquel Francisco ◽

Stephen A Kania

Keyword(s):

Nucleotide Sequence ◽

Dna Sequences ◽

The Other ◽

Total Nucleic Acid ◽

Inflammatory Dermatoses ◽

Formalin Fixed Paraffin ◽

Primer Sets ◽

Unrelated Adult ◽

Background – Corns are hard protuberances that occur on the digital footpads of Greyhound dogs. The cause of these lesions is unknown and there is little information about them in the veterinary literature. We received anecdotal examples of dog to dog spread of corns suggesting an infectious cause. The aim of this study was to determine if papillomavirus (PV) is associated with Greyhound corns. Methods – We examined four corns from two unrelated adult Greyhound dogs that resided in Florida and Washington, respectively, for PV by PCR. The samples were obtained by owner coring of two lesions from one dog and laser removal of two lesions from the other dog. Total nucleic acid was extracted and DNA was amplified using two PCR primer sets that have been shown to amplify a broad range of PVs from humans and animals: FAP59/ FAP64 and MY11/ MY09. The DNA sequences were compared with all sequences in GenBank. Formalin-fixed, paraffin-embedded tissue from the footpads of four dogs with other inflammatory dermatoses were also examined. Results – PV DNA was amplified from all four corn lesions, while no PV DNA was amplified from other tissues. Comparison of the 300-400-bp sequences amplified by the MY11/ MY09 primers identified two different PVs. One showed 96% nucleotide sequence homology with the L1 gene of canine PV type 12. The other showed 78% homology to canine PV type 16, and, therefore, represents a novel PV. In one of the corns, infection by two of the identified PVs was found. Discussion – These results suggest PV infection could be involved in the pathogenesis of corns in Greyhound dogs.

Different Assays for Her-2/neu Evaluation in Breast Cancer

Tumori Journal ◽

10.1177/030089169207800208 ◽

1992 ◽

Vol 78 (2) ◽

pp. 106-110 ◽

Cited By ~ 3

Author(s):

Stefania Tommasi ◽

Angelo Paradiso ◽

Gabriella Serio ◽

Anna Barletta ◽

Anita Mangia ◽

...

Keyword(s):

Breast Cancer ◽

Western Blot ◽

Operable Breast Cancer ◽

The Other ◽

Primary Tumors ◽

Formalin Fixed Paraffin ◽

Her 2 ◽

Pair Analysis ◽

To evaluate different methodologic approaches for HER-2/neu analysis, we performed Southern, Northern, Western blot and histochemical assay on 112 samples from 86 primary tumors and 26 synchronous axillary metastatic lymph nodes of patients affected by operable breast cancer. Simultaneous statistical analysis of data obtained with the four methods (31 samples) showed that Western blot detected a higher percentage of alterations than the other assays (Cochran and Victor tests, 0.01 < p < 0.05). The same result was emphasized by pair analysis (McNemar, p < 0.05), which evaluated the assay data two by two. Immunohistochemical evaluations were more in accord with immunoprecipitation data when performed on frozen or Bouin-fixed, paraffin-embedded tissues than on formalin-fixed, paraffin-embedded tissues.

Phylogenetic analysis of Histoplasma capsulatum var duboisii in baboons from archived formalin-fixed, paraffin embedded tissues

Medical Mycology ◽

10.1093/mmy/myy003 ◽

2018 ◽

Vol 57 (2) ◽

pp. 256-259

Author(s):

M Hensel ◽

A Rodrigues Hoffmann ◽

M Gonzales ◽

M A Owston ◽

E J Dick

Keyword(s):

Dna Sequences ◽

Histoplasma Capsulatum ◽

Granulomatous Disease ◽

African Countries ◽

Archived Samples ◽

Formalin Fixed Paraffin ◽

Ffpe Tissues ◽

African Clade ◽

AbstractHistoplasma capsulatum var. duboisii (Hcd) infections have been well documented to cause chronic granulomatous disease, mainly involving the skin of baboons and humans in African countries primarily. This retrospective study classified the subspecies of Histoplasma and developed a phylogenetic tree utilizing DNA sequences extracted from formalin-fixed, paraffin embedded (FFPE) tissues from 9 baboons from a research colony in Texas histologically diagnosed with Hcd. Based on sequence analysis of ITS-2, Tub-1, and ARF, Hcd isolated from the archived samples closely aligns with the African clade and has 88% sequence homology with a sample isolated from an individual in Senegal.

The Utility of the BIOMED-2 Primers in the Detection of 2 Clonal, B-Lymphoproliferative Disorders Simultaneously Involving the Same Site

Archives of Pathology & Laboratory Medicine ◽

10.5858/arpa.2012-0666-oa ◽

2013 ◽

Vol 137 (11) ◽

pp. 1654-1659 ◽

Cited By ~ 5

Author(s):

Brenda Ly ◽

Claudiu V. Cotta

Keyword(s):

Monoclonal Gammopathy ◽

Tissue Sample ◽

Lymphoproliferative Disorders ◽

Molecular Tests ◽

Formalin Fixed Paraffin ◽

Primer Sets ◽

Formalin Fixed ◽

Undetermined Significance ◽

Abnormal Results

Context.—Molecular tests for clonality performed on atypical lymphoid lesions may yield abnormal results because of the coexistence of monoclonal B lymphocytosis or monoclonal gammopathy of undetermined significance in the sample investigated. Objective.—To investigate the ability of the BIOMED-2 sets of primers to identify 2 clonal populations in the same formalin-fixed, paraffin-embedded tissue sample. Design.—Ten cases with 2 B-lymphoproliferative disorders at the same site were analyzed using 5 BIOMED-2 primer sets (IGH FR1, FR2, FR3, IGK VJ, and IGK VKde). Results.—All 10 cases (100%) showed at least 1 clone; 8 of 10 cases (80%) had 2 clones with at least 1 primer set, and the 2 clones were shown by 4 or 5 primer sets in none of the cases (0%), by 3 sets in 1 of 10 cases (10%), by 2 sets in 4 of 10 cases (40%), and by 1 set in 3 of 10 cases (30%). The most effective set was IGH FR2, detecting 4 of 10 biclonal cases (40%). The IGK VJ and IGK VKde each showed 2 clones in 3 of 10 cases (30% each). The least effective sets were IGH FR1 and FR3, with 2 of 10 cases (20%) each, with IGH FR1 being the least useful. Conclusions.—The BIOMED-2 primers are effective in the detection of 2 clonal populations in the same sample.

Evaluation of the Anyplex II HPV28 Assay in the Detection of Human Papillomavirus in Archival Samples of Oropharyngeal Carcinomas

Archives of Pathology & Laboratory Medicine ◽

10.5858/arpa.2019-0199-oa ◽

2019 ◽

Vol 144 (5) ◽

pp. 620-625 ◽

Cited By ~ 2

Author(s):

Francesca Rollo ◽

Maria Gabriella Donà ◽

Barbara Pichi ◽

Raul Pellini ◽

Renato Covello ◽

...

Keyword(s):

Human Papillomavirus ◽

Detection Rate ◽

The Other ◽

Hpv Dna ◽

Formalin Fixed Paraffin ◽

Hpv Status ◽

Good Concordance ◽

Cancer Tissues ◽

Context.— The improved survival and better response to treatments of human papillomavirus (HPV)–related oropharyngeal squamous cell carcinoma (OPSCC) highlight the need for effective tools in evaluating HPV status on formalin-fixed, paraffin-embedded (FFPE) cancer tissues. To date, there is no agreement regarding the most appropriate method for HPV testing on FFPE materials. Objective.— We aimed to investigate the performance of the Anyplex II HPV28 (Anyplex) on FFPE OPSCC tissues and to compare it with 2 other methods for HPV-DNA detection and p16 overexpression. Design.— One hundred sixty FFPE OPSCCs were evaluated, which had already been analyzed with the INNO-LiPA HPV assay, Xpert HPV assay, and p16 immunostaining. Results.— All the samples but 1 provided valid results with the Anyplex, which showed the highest HPV detection rate and a good concordance with all the other methods (κ = 0.75, 95% CI, 0.65–0.85 versus INNO-LiPA; κ = 0.80, 95% CI, 0.70–0.89 versus Xpert; κ = 0.76, 95% CI, 0.65–0.86 versus p16). Moreover, the HPV-driven fraction, based on HPV-DNA and p16 double positivity, was higher with Anyplex (83 of 159, 52.2%) than with the other 2 assays, that is, 78 of 156 (50.0%) for INNO-LiPA and 80 of 160 (50.0%) for Xpert. Conclusions.— Anyplex II HPV28 showed a higher HPV detection rate and HPV-associated fraction than the other methods used. This assay is suitable for HPV detection in archival OPSCC tissues.

Cutaneous Sarcoids in Captive African Lions Associated With Feline Sarcoid-Associated Papillomavirus Infection

Veterinary Pathology ◽

10.1177/0300985810391111 ◽

2010 ◽

Vol 48 (6) ◽

pp. 1176-1179 ◽

Cited By ~ 19

Author(s):

G. M. B. Orbell ◽

S. Young ◽

J. S. Munday

Keyword(s):

Dna Sequences ◽

Skin Lesions ◽

Cutaneous Lesions ◽

Clinically Normal ◽

Papillomavirus Infection ◽

Formalin Fixed Paraffin ◽

Polymerase Chain ◽

African Lions ◽

Solitary and multiple cutaneous and mucocutaneous masses were identified in 5 of 24 captive African lions ( Panthera leo) over a 6-month-period. All masses were surgically excised, and all were histologically similar to equine and feline sarcoids. DNA was extracted from formalin-fixed, paraffin-embedded tissue. Polymerase chain reaction amplified DNA sequences that had been previously detected in feline sarcoids and clinically normal bovine skin. All lions had been fed a diet that included bovine carcasses that had not been skinned. Since the cessation of feeding bovine carcasses with cutaneous lesions, no additional skin lesions have been observed within any of the lions. Herein is described the clinical, gross, and histopathological findings of sarcoids in 5 captive lions. As the causative papillomavirus most likely has a bovine definitive host, it is hypothesized that the lions were exposed to the virus by feeding on bovine carcasses with skin still attached.