Actinomyces pyogenes isolates from Sheep: Biochemical identification and confirmation by molecular method

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).

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BIOCHEMICAL IDENTIFICATION OF PATOVARS OF THE PATHOGEN OF VASCULAR BACTERIOSIS OF THE CRUCIFEROUS XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS

SCIENTIFIC LIFE ◽

10.35679/1991-9476-2020-15-1-82-88 ◽

2020 ◽

Vol 15 (1) ◽

pp. 82-88

Author(s):

Mikhail Kuznetsov ◽

◽

Anatoly Scherbakov ◽

Elena Gorelnikova ◽

Nadezhda Chervyakova ◽

...

Keyword(s):

Xanthomonas Campestris ◽

Biochemical Identification ◽

Xanthomonas Campestris Pv Campestris

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In-depth biochemical identification of a novel methyl parathion hydrolase from Azohydromonas australica and its high effectiveness in the degradation of various organophosphorus pesticides

Bioresource Technology ◽

10.1016/j.biortech.2020.124641 ◽

2021 ◽

pp. 124641

Author(s):

Sumao Zhao ◽

Wei Xu ◽

Wenli Zhang ◽

Hao Wu ◽

Cuie Guang ◽

...

Keyword(s):

Methyl Parathion ◽

Organophosphorus Pesticides ◽

Methyl Parathion Hydrolase ◽

High Effectiveness ◽

Biochemical Identification ◽

Parathion Hydrolase ◽

Azohydromonas Australica

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Seewis hantavirus in common shrew (Sorex araneus) in Sweden

Virology Journal ◽

10.1186/s12985-020-01477-w ◽

2020 ◽

Vol 17 (1) ◽

Author(s):

Olivia Wesula Lwande ◽

Nahla Mohamed ◽

Göran Bucht ◽

Clas Ahlm ◽

Gert Olsson ◽

...

Keyword(s):

Common Shrew ◽

Hemorrhagic Fever ◽

Sorex Araneus ◽

Genome Segment ◽

Cytochrome C Oxidase I ◽

Emerging Viruses ◽

Molecular Method ◽

Typing Method ◽

Common Shrew Sorex Araneus ◽

Genetic Assay

Abstract Background Rodent borne hantaviruses are emerging viruses infecting humans through inhalation. They cause hemorrhagic fever with renal syndrome and hemorrhagic cardiopulmonary syndrome. Recently, hantaviruses have been detected in other small mammals such as Soricomorpha (shrews, moles) and Chiroptera (bats), suggested as reservoirs for potential pandemic viruses and to play a role in the evolution of hantaviruses. It is important to study the global virome in different reservoirs, therefore our aim was to investigate whether shrews in Sweden carried any hantaviruses. Moreover, to accurately determine the host species, we developed a molecular method for identification of shrews. Method Shrews (n = 198), caught during 1998 in Sweden, were screened with a pan-hantavirus PCR using primers from a conserved region of the large genome segment. In addition to morphological typing of shrews, we developed a molecular based typing method using sequencing of the mitochondrial cytochrome C oxidase I (COI) and cytochrome B (CytB) genes. PCR amplified hantavirus and shrew fragments were sequenced and phylogenetically analysed. Results Hantavirus RNA was detected in three shrews. Sequencing identified the virus as Seewis hantavirus (SWSV), most closely related to previous isolates from Finland and Russia. All three SWSV sequences were retrieved from common shrews (Sorex araneus) sampled in Västerbotten County, Sweden. The genetic assay for shrew identification was able to identify native Swedish shrew species, and the genetic typing of the Swedish common shrews revealed that they were most similar to common shrews from Russia. Conclusion We detected SWSV RNA in Swedish common shrew samples and developed a genetic assay for shrew identification based on the COI and CytB genes. This was the first report of presence of hantavirus in Swedish shrews.

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Application of Lactiplantibacillus plantarum SCH1 for the Bioconservation of Cooked Sausage Made from Mechanically Separated Poultry Meat

Applied Sciences ◽

10.3390/app11041576 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1576

Author(s):

Beata Łaszkiewicz ◽

Piotr Szymański ◽

Dorota Zielińska ◽

Danuta Kołożyn-Krajewska

Keyword(s):

Sodium Nitrite ◽

Sensory Quality ◽

Microbiological Quality ◽

Poultry Meat ◽

Control Treatment ◽

Nitrite Level ◽

Negative Effect ◽

Biochemical Identification ◽

Nitrates Content ◽

Physical And Chemical

The aim of the research was an assessment of the effect of the Lactiplantibacillus plantarum SCH1 strain isolated from ecological raw fermented pork roast on pH, redox potential, nitrites, and nitrates content, L a* b* color parameters, total heme pigments content, nitrosyl myoglobin concentration, as well as the microbiological quality and sensory traits of cooked sausages produced from mechanically separated poultry meat (MSPM), cured with a lower sodium nitrite level (NaNO2 50 mg/kg) after production as well as after storage (1 and 3 weeks of storage). The biochemical identification of the Lactobacillus bacteria after storage was also performed. Tests were performed in two sausage treatments: C—control sausage made from MSPM and L—sausage made from MSPM inoculated with L. plantarum at approx. 107 cfu/g. No negative effect of using the L. plantarum SCH1 strain on the physical and chemical MSPM sausage features was found. The treatment with L. plantarum SCH1 was of better microbiological quality after 3 weeks of storage. The sausages with L. plantarum SCH1 kept good sensory quality while the control treatment was spoiled after storage.

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Salmonella enterica Subsp. houtenae Associated with an Abscess in Young Roe Deer (Capreolus capreolus)

Pathogens ◽

10.3390/pathogens10060654 ◽

2021 ◽

Vol 10 (6) ◽

pp. 654

Author(s):

Adriana Trotta ◽

Laura Del Sambro ◽

Michela Galgano ◽

Stefano Ciccarelli ◽

Erika Ottone ◽

...

Keyword(s):

Salmonella Enterica ◽

Roe Deer ◽

Capreolus Capreolus ◽

Epidemiological Data ◽

Resistance Pattern ◽

Salmonella Spp ◽

Antibiotic Resistant ◽

Biochemical Identification ◽

Salmonella Pathogenicity Islands ◽

Insight Into

Background: S. enterica subsp. houtenae has been rarely documented, and very limited genomic information is available. This report describes a rare case of primary extraintestinal salmonellosis in a young roe deer, associated with Salmonella enterica subsp. houtenae. Methods: A traditional cultural-based analysis was carried out from the contents of a neck abscess; biochemical identification and PCR assay were performed to isolate and identify the pathogen. Through whole-genome sequencing (WGS), multilocus sequence typing (MLST), core genome MLST (cgMLST), and the Salmonella pathogenicity islands (SPIs) survey, resistome and virulome genes were investigated to gain insight into the virulence and antimicrobial resistance of S. houtenae. Results: Biochemical identification and PCR confirmed the presence of Salmonella spp. in the swelling. The WGS analysis identified Salmonella enterica subspecies houtenae serovar 43:z4,z23:- and ST 958. The virulence study predicted a multidrug resistance pattern with resistance shown against aminoglycosides, tetracycline, beta-lactamase, fluoroquinolones, fosfomycin, nitroimidazole, aminocoumarin, and peptide. Fifty-three antibiotic-resistant genes were identified. No plasmids were detected. Conclusion: This study demonstrates the importance of continuous surveillance of pathogenic salmonellae. Biomolecular analyses combined with epidemiological data can provide important information about poorly described Salmonella strains and can help to improve animal welfare.

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