scholarly journals Salmonella enterica Subsp. houtenae Associated with an Abscess in Young Roe Deer (Capreolus capreolus)

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 654
Author(s):  
Adriana Trotta ◽  
Laura Del Sambro ◽  
Michela Galgano ◽  
Stefano Ciccarelli ◽  
Erika Ottone ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Roe Deer ◽  
Capreolus Capreolus ◽  
Epidemiological Data ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
Biochemical Identification ◽  
Salmonella Pathogenicity Islands ◽  
Insight Into

Background: S. enterica subsp. houtenae has been rarely documented, and very limited genomic information is available. This report describes a rare case of primary extraintestinal salmonellosis in a young roe deer, associated with Salmonella enterica subsp. houtenae. Methods: A traditional cultural-based analysis was carried out from the contents of a neck abscess; biochemical identification and PCR assay were performed to isolate and identify the pathogen. Through whole-genome sequencing (WGS), multilocus sequence typing (MLST), core genome MLST (cgMLST), and the Salmonella pathogenicity islands (SPIs) survey, resistome and virulome genes were investigated to gain insight into the virulence and antimicrobial resistance of S. houtenae. Results: Biochemical identification and PCR confirmed the presence of Salmonella spp. in the swelling. The WGS analysis identified Salmonella enterica subspecies houtenae serovar 43:z4,z23:- and ST 958. The virulence study predicted a multidrug resistance pattern with resistance shown against aminoglycosides, tetracycline, beta-lactamase, fluoroquinolones, fosfomycin, nitroimidazole, aminocoumarin, and peptide. Fifty-three antibiotic-resistant genes were identified. No plasmids were detected. Conclusion: This study demonstrates the importance of continuous surveillance of pathogenic salmonellae. Biomolecular analyses combined with epidemiological data can provide important information about poorly described Salmonella strains and can help to improve animal welfare.

scholarly journals Effects of Animal, Climatic, Hunting and Handling Conditions on the Hygienic Characteristics of Hunted Roe Doer (Caprelous capreolus L.)

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1076
Author(s):  
Raffaella Branciari ◽  
Andrea Onofri ◽  
Fausto Cambiotti ◽  
David Ranucci
Keyword(s):  
Listeria Monocytogenes ◽  
Roe Deer ◽  
Control Strategies ◽  
Capreolus Capreolus ◽  
Careful Consideration ◽  
Salmonella Spp ◽  
Climate Conditions ◽  
Factors Affecting ◽  
And Control ◽  
Carcass Contamination

The population of wild animals is increasing, and control strategies based on selective hunting are among the major options adopted. The game meat obtained is therefore available for controlled and certified valuable chains. The understanding of carcass contamination and the factors affecting it is therefore crucial to ensure meat safety and prolonged shelf-life. The carcass hygiene of 64 hunted wild male roe deer (Capreolus capreolus L.) was evaluated in relation to factors potentially affecting it. Aerobic colony and Enterobacteriaceae counts, as well as Salmonella spp. and Listeria monocytogenes detection, were performed. The interaction of the microbial determination with age and weight of the animals, the climate conditions, the shooting procedure, the time between the killing and the evisceration as well as the time of storage of the carcasses in refrigerated conditions before skinning, were evaluated. Neither Salmonella spp. nor Listeria monocytogenes were detected on the carcasses and the average loads detected were 3.39 ± 1.06 UFC/cm2 and 2.27± 1.11 UFC/cm2 for the aerobic colony count and Enterobacteriaceae count, respectively. The loads detected are similar to those reported by UE legislation for slaughtered species. The time of storage before skinning, the environmental temperature during hunting and the time between shooting and evisceration, associated with animal weight, affect the carcass hygiene and must be taken into careful consideration by hunters as food business operators.

scholarly journals Characterization of Antibiotic Resistant Salmonella spp Isolated from Chicken Eggs of Dhaka City

2010 ◽  
Vol 3 (1) ◽  
pp. 191 ◽  
Author(s):  
M. M. Ahmed ◽  
M. M. Rahman ◽  
K. R. Mahbub ◽  
M. Wahiduzzaman
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Egg Yolk ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
Poultry Eggs ◽  
Potential Reservoir ◽  
Egg Shell ◽  
Chicken Eggs

In present study, the occurrence of Salmonella in local chicken eggs and their pattern of antibiotic resistance were determined. 100 egg samples collected from different locations of Dhaka city were analysed and Salmonella spp were found in 8% of the samples. Among all presumptive Salmonella isolates, 8 isolates were confirmed as Salmonella enterica subsp. salamae (4%), Salmonella enterica subsp. indica (1%), Salmonella Paratyphi-A (1%), Salmonella bongori (1%) and Salmonella Choleraesuis (1%) on the basis of serotyping and biochemical analysis. These isolates were subjected to susceptibility test against 10 antibiotic disks. All the isolates were found chloramphenicol sensitive. The highest percentage of resistance (87.5%) was found to amoxicillin and ampicillin. Resistance against erythromycin, cephalexin, doxycycline hydrochloride, ceftazidime, doxycycline and nalidixic acid was also found significant ranging from 25% to 62.5%. Salmonella isolated from egg shell surface were found more antibiotic resistant than that of egg yolk and white. The present study suggests that poultry eggs are potential reservoir of antibiotic resistant Salmonellae.Keywords: Egg; Salmonella; Characterization; Antibiotic resistance.© 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi:10.3329/jsr.v3i1.6109                J. Sci. Res. 3 (1), 191-196  (2011)

scholarly journals An Investigation of Antibacterial Resistance Patterns in Isolated Bacteria from Contaminated Water Samples in Poultry Slaughterhouses

2020 ◽  
Vol 1 (2) ◽  
pp. 85-90
Author(s):  
Hadis Tavafi
Keyword(s):  
Antibiotic Resistance ◽  
Water Samples ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Contaminated Water ◽  
Antibiotic Resistant Bacteria ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
Resistance Patterns ◽  
Klebsiella Spp

Nowadays, in the poultry industry, antibiotics are used to treat, prevent, and enhance poultry growth and production efficiency. Their irregular consumption has resulted in the spread of antibiotic-resistant bacteria in this industry. Antibiotic-resistant bacteria in contaminated waters can be transmitted into soil. The purpose of this study was to investigate the antibiotic resistance pattern of bacteria isolated from the water of chicken slaughterhouses around Hamadan (Iran) province. In this study, 20 water samples were collected from four slaughterhouses in Hamadan province (during spring and summer 2019). Initial isolation and identification of the bacteria were performed by pour plate culture and biochemical tests. The disc diffusion method was applied to investigate the resistance pattern. This study presents 109 screened isolates. Of these, 57.8% E.coli, 35.7% Salmonella spp., and 6.42% Klebsiella spp. were detected. Antibiograms of isolates showed that in E.coli, 23.09% were resistant to four types of the antibiotic tetracycline, amoxicillin, gentamicin, and chloramphenicol, 76.19% had only one type of antibiotic. Antibiotics for Salmonella spp. showed that 35.9% were resistant to tetracycline, gentamicin, and chloramphenicol, 64.10% to only one type of antibiotic. Also, in Klebsiella spp., 85.71% were sensitive to antibiotics, and only 14.28% were resistant to tetracycline. Conclusion: The results showed that the rate of multiple antibiotic resistance is relatively high, and contaminated water has a high potential for soil contamination. Therefore, resistant bacteria become more stable in the environment, and the health of the environment will be endangered. Therefore, it is necessary to study the antimicrobial resistance patterns of bacteria to study and maintain the health of the environment.

scholarly journals Evaluation of Class 1 and 2 Integrons and Antibiotic Resistance Pattern in Salmonella enterica Isolated from Diarrheal Food-Borne Outbreaks in Iran

2019 ◽  
Author(s):  
S.F. Sayadnouri ◽  
M.M. Soltan Dallal ◽  
S. Akbarzadeh ◽  
R. Mazaheri Nezhad Fard
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Control Measures ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Chi Square ◽  
Class 1 Integron ◽  
Antibiotic Resistance Pattern ◽  
Food Borne ◽  
Class 1

Background: Salmonella spp. are major causes of food-borne disease and have been identified among many diarrheal outbreaks. The major aim of the current investigation was to evaluate the class 1 and 2 integrons and antibiotic resistance pattern in Salmonella enterica isolated from diarrheal food-borne outbreaks in Iran.  Methods: This study was carried out on 115 diarrheal feces samples obtained from food-borne outbreak in 2016 in Iran. Antimicrobial resistance patterns of 27 isolated S. enterica seovars and presence of class 1 and class 2 integrons in the serovars were investigated using conventional and molecular methods. Results were statistically analyzed using SPSS software v. 21 and Chi-Square test. Results: Overall, 27 S. enterica were characterized as 14 S. Paratyphi C, 7 S. Enteritidis, 5 S. Paratyphi D, and 1 S. Paratyphi A serovars. Results of molecular assay showed that class 1 integron presented in all and class 2 integron in three strains. All isolates with class 2 integron genes were resistant to almost all the antimicrobials. Conclusion: Most studied Salmonella strains from diarrheal food-borne outbreak of Iran in 2016 were multiple resistant to the highlighted antimicrobials. Knowledge about risk factor involving the salmonellosis and their control measures could help the national authorities to prevent the outbreaks. Further comprehensive studies with larger sample sizes are necessary to acquire more data about risk factors of multiple resistant Salmonella outbreaks in the country.

scholarly journals Microbial, chemical-physical, rheological and organoleptic characterisation of roe deer (Capreolus capreolus) salami

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
David Ranucci ◽  
Rossana Roila ◽  
Dino Miraglia ◽  
Chiara Arcangeli ◽  
Francesca Vercillo ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Rural Areas ◽  
Roe Deer ◽  
Meat Products ◽  
Capreolus Capreolus ◽  
Lipid Levels ◽  
Salmonella Spp ◽  
Local Economies ◽  
Game Meat ◽  
The Difference

Game meat and related products are important in the promotion of local economies and rural areas. Microbiological, chemical–physical, rheological and sensory characteristics of fermented meat products (salami) made by different percentages of pork and hunted roe-deer (Capreolus capreolus) meat were evaluated. The microbiological determination indicated that the products are safe to eat, as neither Listeria monocytogenes nor Salmonella spp. was isolated from the samples. The hygienic adequacy of the process was guaranteed, as there was below 3 log CFU/g of Enterobacteriaceae level in the final products. The proximal composition analyses showed lower lipid levels in comparison to pork salami. The difference in chemical composition affects the rheological and sensory traits of the final products; the products were harder and with higher gumminess when 50% of roe-deer meat was used. Game meat flavour and odour increased with the increasing percentage of roe-deer meat.

scholarly journals Isolation, Identification, and Antibiotic Sensitivity Pattern of Salmonella spp from Locally Isolated Egg Samples

2019 ◽  
pp. 1-11 ◽  
Keyword(s):  
Antibiotic Susceptibility ◽  
Public Health Problem ◽  
Resistance Pattern ◽  
Human Consumption ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
Poultry Eggs ◽  
Sensitivity Pattern ◽  
Antibiotic Susceptibility Test

Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered as major sources for these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg sample collected from different retailer markets and farm of Savar area, Bangladesh. Antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from eggs yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found to Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from egg surface were found more antibiotic resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.

scholarly journals Phenotype and Molecular Characterization of Antibiotic Resistance of Salmonella spp. from Cattle in Abidjan District, Côte d'Ivoire

2019 ◽  
pp. 1-10
Author(s):  
Coulibaly Kalpy Julien ◽  
Yao Kouamé René ◽  
Dagnogo Komissiri ◽  
Tiékoura Konan Bertin ◽  
Koffi Kouassi Eugène ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Cow Dung ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
Strain Isolation ◽  
Healthy Cattle ◽  
Microbiological Techniques ◽  
Biochemical Identification

Aims: The overall objective of this study was to study antibiotic-resistant strains of Salmonella in livestock sales sites in the Abidjan district. Place and Duration of Study: Researchers team from Institute Pasteur of Ivory Coast and their students collected samples of cattle feces in five townships of the district of Abidjan from April to September 2016. Methodology: Fresh cow dung has been collected from sales outlets and livestock pens in five municipalities in Abidjan. The prevalence of Salmonella carrying has been studied by classical microbiological techniques. These included strain isolation by culture on the Hektoen medium, biochemical identification using Leminor's reduced rack and strain confirmation by MALDI-TOF MS. Phenotypic determination of antibiotic resistance and detection their genes were carried out respectively by the method of discs diffusion on the Muller-Hinton agar and using PCR simplex. Results: In this study, we collected 420 samples. The results showed that the overall prevalence of Salmonella isolated from cattle feceswas 20% (84/420). Twenty-six (26) strains were resistant to at least one antibiotic. The blaCTX-M, blaSHV, blaTEM and TetA resistance genes were detected with the respective frequencies of 7.7%, 57.7% and 7.7%. Conclusion: The isolation of antibiotic-resistant Salmonella strains in these healthy cattle poses a significant threat to public health. However, a good use of antibiotics in farms could help limit the phenomenon of resistance of Salmonella to antibiotics.

scholarly journals Detection of Zoonotic Antibiotic Resistant Salmonella spp. Carrying Virulence Genes in Rural Mus musculus, Golestan Province, North of Iran

2019 ◽  
Vol 6 (3) ◽  
pp. 96-100
Author(s):  
Somayeh Namroodi
Keyword(s):  
Antibiotic Resistance ◽  
Mus Musculus ◽  
Rural Areas ◽  
Virulence Genes ◽  
Native People ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
Climate Conditions ◽  
Golestan Province

Background and aims: Salmonella spp. infect cold-blooded and warm-blooded animals and may cause a worldwide zoonotic disease, salmonellosis, in infected animals. Rodents can be Salmonella carriers without any signs of salmonellosis. The frequency of salmonella contamination, the presence of virulence genes (SpvR and SpvB) and antibiotic resistance pattern of isolated Salmonella were studied in rural Mus musculus to reveal the possible role of them in Salmonella dissemination in Golestan province. Methods: A total of 190 wet rectal swabs of M. musculus were obtained from rural areas of Golestan province with different climate conditions. The swabs were cultured and positive samples were serotyped and their antibiotic resistance patterns were studied. The presence of Salmonella and virulence genes was analyzed by SpvR and SpvB genes primers, respectively. Results: Salmonella spp. were detected in 15 (7%) of 190 fecal samples by bacterial culture and PCR. S. enteritidis (n=9) and S. typhimurium (n=6) were identified. The highest and lowest frequencies were detected in humid (13.1%) and arid areas (3.1%), respectively. SpvR and SpvB genes were diagnosed in 10 of 15 (66.6%) isolated Salmonella. The highest resistance of detected Salmonella spp. was observed against streptomycin (53%). All the isolates were sensitive to chloramphenicol, gentamicin and trimethoprim. Conclusion: The Salmonella contamination in sampled house mice indicates that native people should be made aware of the risk of Salmonella infection and possible ways of salmonella transmission through rodents. In addition, the application of appropriate therapeutic approaches to prevent the spread of antibiotic resistant Salmonella is recommended.

Enamel Thickness Differs between Field and Forest European Roe Deer Capreolus capreolus

2020 ◽  
Vol 68 (1) ◽  
pp. 100
Author(s):  
Jan Demesko ◽  
Marta Kurek ◽  
Patrycja Podlaszczuk ◽  
Janusz Markowski
Keyword(s):  
Roe Deer ◽  
Capreolus Capreolus ◽  
Enamel Thickness
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