Identification Pseudomonas aeruginosa by OprD Gene for Differentiation from Other Pseudomonas Species that Isolated from Clinical Samples

2019 ◽  
Vol 9 (01) ◽  
pp. 46-50
Author(s):  
Ashwak B Al-Hashimy ◽  
Huda S Alagely ◽  
Akeel K Albuaji ◽  
Khalid R Majeed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
General Hospital ◽  
Culture Media ◽  
Final Diagnosis ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Molecular Method ◽  
Biochemical Tests ◽  
Pseudomonas Species ◽  
Specific Sequences

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).

Bacteriological Study of Pseudomonas aeruginosa Isolated from Tonsillitis Patients

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Zahraa Hameed Oda Alquraishi ◽  
Israa Abdul Ameer Al-Kraety ◽  
Aqeel A. Alsadawi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Microscopic Study ◽  
Clinical Samples ◽  
Molecular Method ◽  
Biochemical Tests ◽  
Bacteriological Study ◽  
Study Results ◽  
Pcr Method ◽  
Vitek 2 ◽  
Physiological And Biochemical

Present study included (50) clinical samples were collected from patients suffering from tonsillitis signs during the period from) November 2018 to January, 2019). All specimens were cultured for microscopic and microscopic study. Results show that from 50 patients, male was 60% and 40% were female. Several morphological, physiological and biochemical tests showed that P. aeruginosa constituted 18 isolates (36%) of these isolates. P. aeruginosa isolates were 18 isolates diagnosed by the morphological ,cultural and biochemical characters, the identification was confirmed by automated VITEK-2 compact system and molecular method for the presence of OprL. The results showed that only 12 (66.6%) isolates diagnosed as P. aeruginosa by automated VITEK-2 compact system and that was carrying OprL which are diagnosed as P. aeruginosa by P.C.R. According to the different diagnostic above, VITEK and PCR method were more sensitivity for P. aeruginosa detection among tonsillitis patients

scholarly journals Study of some Virulence Factors for Clostridium perfringens isolated from Clinical Samples and Hospital Environment and showing their Sensitivity to Antibiotics

2020 ◽  
Vol 11 (02) ◽  
pp. 253-256
Author(s):  
Zainab I Tahseen ◽  
Muhsin H. Edham ◽  
Asma S. Karomi
Keyword(s):  
General Hospital ◽  
Virulence Factors ◽  
Clostridium Perfringens ◽  
High Resistance ◽  
High Sensitivity ◽  
Hospital Environment ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
The City

The study included taking 100 samples from different clinical sources, including wounds and burns, and from the hospital environment, in Kirkuk General Hospital and Azadi Teaching Hospital in the city of Kirkuk for the period from November 2017 to August 2018. The results of isolation and diagnosis showed the growth of 30 isolates that are positive for Clostridium perfringens, distributed between 15 isolates 37.5% from burns, 11 isolates 27.5% from wounds, and 4 isolates 20% from the hospital environment. These isolates were diagnosed based on microscopical, cultural and biochemical tests, in addition to being diagnosed with the Api 20A system. The sensitivity of isolates was tested toward a number of types of antibiotics, and all bacterial isolates showed a high sensitivity 100% against imipenem. As for the sensitivity to vancomycin, amikacin, tetracycline was 96.66, 90, and 66.66% respectively. While, all isolates showed a high resistance to metronidazole and colistin 100%, some virulence factors of C. perfringens isolates have been studied , and showed that all isolates (%100) have the ability to produce hemolysin, lecithinase, capsule, and spore, while 70% of the isolates produced DNAase.

scholarly journals Genotyping of fusA Gene from Clinical Isolates Acinetobacter baumannii in Baghdad

2018 ◽  
Vol 15 (1) ◽  
pp. 37-42
Author(s):  
Baghdad Science Journal
Keyword(s):  
Phylogenetic Tree ◽  
Acinetobacter Baumannii ◽  
Laboratory Diagnosis ◽  
Clinical Isolates ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Coding Region ◽  
Biochemical Tests ◽  
Amplification Process ◽  
Group A

This study aims at detecting the differences in genotyping of coding region fusA gene in clinical isolates of Acinetobacter baumannii from Baghdad, Iraq. Collected two hundred clinical samples (50 samples from urine, 50 samples from wound, 50 samples from sputum and 50 samples from otitis infections). Laboratory diagnosis for bacterial isolates carried out by some biochemical tests and confirmed by using VITEK- 2 compact system. The results appeared that twenty isolates of Acinetobacter baumannii in all these samples. Genotyping study was performed of coding region fusA gene of the extracted genome of all bacterial isolates and used specific primers in achieved amplification process of this target gene. DNA sequencing of this gene and alignment of sequencing in NCBI was achieved and drew phylogenetic tree by using Geneious 9 software among locally isolates alone and then among locally isolates and high identity global isolates in GenBank. The results in phylogenetic tree of fusA gene in locally isolates showed 4 groups of isolates included more than one source of isolation. The results in phylogenetic tree of the locally and global isolates showed that are four different groups and each group included some locally isolates and global isolates except group A (AE_22, AE_26) and group E (AE_35, AE_32, AE_33) that not identity with global isolates. The nucleotides sequence of fusA gene from localized isolate (AE_35) was registered in national GenBank under accession number (LOCUS KY818057) and protein ID "ARV90995.1.

scholarly journals PREVALENCE AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF PSEUDOMONAS SPECIES ISOLATED FROM CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL

2021 ◽  
pp. 50-53
Author(s):  
M. ABDUR RAHMAN ◽  
POOJA NAIR
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care ◽  
Control Measures ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Clinical Samples ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Pseudomonas Species

Objective: Pseudomonas aeruginosa is the commonest agent causing opportunistic nosocomial infections, contributing to mortality and morbidity worldwide. Along with its intrinsic and acquired resistance mechanisms, infections caused by Pseudomonas species further lead to treatment failure. This study was done to determine the prevalence and antimicrobial susceptibility pattern of different species of Pseudomonas isolated from various clinical samples by phenotypic methods. Methods: This study involved the examination of clinical samples for various species of Pseudomonas by using different standard biochemical tests. Their antimicrobial susceptibility profile was performed by the Kirby Bauer disc diffusion method. Analysis of the antibiogram pattern was done to study the multi-drug resistance among Pseudomonas species. Results: Out of the 1249 bacterial isolates, Pseudomonas species accounted for 12.9%. Pseudomonas species showed maximum resistance to gentamicin (31.4%), followed by ciprofloxacin (30.2%. and showed the least resistance tocolistin and polymyxin B. 46 isolates out of the 162 (28%) were found to be Multi-Drug Resistant (MDR) Pseudomonas aeruginosa. Conclusion: The present study highlights that Pseudomonas species remains a major cause of hospital-acquired infections. Multidrug resistance was observed in most of the strains, which makes the therapeutic options more difficult. Surveillance of antimicrobial resistance and strict infection control measures are essentially to be practiced in managing and control of infections caused by Pseudomonas.

scholarly journals Isolation of Pseudomonas species from soil sample for production of Pyoverdine and evaluation of its potential as an antimicrobial agent

2021 ◽  
pp. 355-361
Author(s):  
Didhiti Agarwal ◽  
Dhevang Rokkala ◽  
Shreyas Tawde ◽  
Prachi Bhatia ◽  
Sejal Rathod
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agent ◽  
Pseudomonas Putida ◽  
Soil Sample ◽  
Growth Conditions ◽  
Biochemical Tests ◽  
Iron Deficient ◽  
Pseudomonas Species ◽  
Fe Complex ◽  
Ecological Habitats

Pyoverdine, a fluorescent siderophore that have high- affinity for iron is produced by Pseudomonas putida and Pseudomonas aeruginosa that synthesizes it under iron-deficient growth conditions. Pseudomonas species are often encountered in diverse ecological habitats along side being censurable for nosocomial infections spread round the world. Due to these characteristics, there's a growing interest during this microbe for a spread of uses. One such is the production of Pyoverdine, it influences the uptake of iron, along with eliminating the limited iron availability condition. Pyoverdine is important for Pseudomonas putida and Pseudomonas aeruginosa to cause acute infections. Intense research and study led to the invention of Pyoverdines being a crucial source of chelating Iron. This study is predicated on Pyoverdine extracted from two different species of Pseudomonas which will act as an antimicrobial agent for various species including Escherichia coli and Staphylococcus aureus. The organism, i.e., Pseudomonas putida and Pseudomonas aeruginosa, were isolated from soil sample using medias like: Cetrimide media, King’s B media. It had been confirmed using primary biochemical tests, along side species level identification (MALDI - TOF). Isolation was followed by studying the antimicrobial activity of Pyoverdine on different organisms using antibiotics as standard for the same. Results for the tests were obtained, colonies were observed on specific media and zone of inhibition was observed on Muller Hinton plate. Comparative studies were carried out to find which organism used up Pyoverdine or Pyoverdine - Fe complex without using FeCl3 as sole standard source. Thus, these compounds can synergize with conventional antimicrobials, forming a simpler treatment with serving as a useful gizmo

scholarly journals Enumeration of the Antimicrobial Susceptibility Patterns of Different Bacterial Isolates from ENT Patients with Ear Infections

2020 ◽  
pp. 68-73
Keyword(s):  
Developing Countries ◽  
Culture Media ◽  
Bacterial Species ◽  
Positive Culture ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Ear Infection ◽  
Pseudomonas Spp ◽  
Multiple Drugs ◽  
Klebsiella Spp

Children are more susceptible to ear infection than adults worldwide, especially in developing countries. In Bangladesh like other developing countries antibiotics is a common choice of treatment in an ear infection. This study was sought to determine causative agents both in right and left ear infection and their antibacterial susceptibility pattern from patients with an ear infection. Specimens of ear discharge were collected aseptically using a sterile swab and cultured on MacConkey’s agar, blood agar, and chocolate agar. After inoculating on appropriate culture media bacterial isolates were identified by their colony morphology, culture characteristics, Gram reaction, and biochemical tests. In this study, a total of 70 positive cases of patients were included. Among these 27 (38.6%) were male, and 43(61.4%) were female. The prevalence of ear infection was significantly high in females (61.4%) than males (38.6%). The predominant bacterial isolates from positive culture were Staphylococcus aureus 43(61.4%), followed by Pseudomonas spp., 13 (18.6%), Streptococcus spp., 8(11.4%), Proteus spp., 5(7.2%), and Klebsiella spp., 1(1.4%). The antibacterial agent like Amikacin and Gentamicin showed a high level of antibacterial effect on all identified bacterial isolates. On the other hand, 98.6% of isolates were found highly resistant to Co-Trimoxazole and Flucloxacillin. Moreover, Streptococcus spp., Proteus spp., Klebsiella spp., and Pseudomonas spp., were highly resistant to multiple drugs (more than 4). A high degree of antibiotic resistance was observed among most of the drugs used in this study. However, Amikacin and Gentamicin were highly effective against the isolated bacterial species. Therefore, culture and susceptibility tests are vital for the appropriate treatment of ear infection.

scholarly journals Frequency of bacterial isolates and antibiotics resistance patterns in urine and pus samples.

2021 ◽  
Vol 28 (11) ◽  
pp. 1571-1577
Author(s):  
Sajid Ali ◽  
Hina Khalid ◽  
Noor Muhammad ◽  
Syed Luqman Shuaib ◽  
Anees Muhammad
Keyword(s):  
Culture Media ◽  
Cross Sectional Study ◽  
Urine Samples ◽  
Antibiotics Resistance ◽  
Klebsiella Pneumonia ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Cross Sectional ◽  
E Coli ◽  
Antibiotic Resistance Profile

Objectives: The present study is aimed to determine frequency of bacteria in urine and pus sample along with the antibiotic resistance profile of isolated bacteria. Study Design: Cross-Sectional Study. Setting: Real-Time PCR Laboratory at Dabgari Garden, Peshawar with the Collaboration of Health Biotechnology Laboratory, Department of Biotechnology, Abdul Wali Khan University, Mardan. Period: June 2018 to July 2019. Material & Methods: The urine and pus samples were collected from suspected patients and were cultured on appropriate culture media. The biochemical tests were also performed after growing on culture plates for identification and confirmation of bacterial isolates. The disc diffusion technique was used to evaluate the antibiotic pattern of retrieved isolates. Result: A total of 525 samples of pus and urine samples were collected from different regions of Peshawar. Out of the total, 237 (45.1%) samples were found positive for bacterial growth whereas the remaining (54.9%) were observed negative. Among total positive isolates, 220 (92.8%) were from urine samples, and 17 (7.2%) were from pus samples. The predominant isolate was Escherichia coli (E. coli) (90.3%) retrieved from positive samples, followed by Pseudomonas aeruginosa (7.2%), Klebsiella pneumonia (1.3%), and Staphylococcus aureus (1.3%). The most effective antibiotic was Fosfomycin against bacteria whereas Nalidixic acid, Nitrofurantoin, and Amoxicillin/clavulanic acid were found less effective against bacterial isolates. Conclusion: Most frequent bacteria isolated was E. coli and the most efficient drug was Fosmomycin and the least was Nitrofurantoin, and Amoxicillin.

scholarly journals Upstream Region of OprD Mutations in Imipenem-Resistant and Imipenem-Sensitive Pseudomonas Aeruginosa Isolates

2020 ◽  
Author(s):  
Masoumeh Kiani ◽  
Akram Astani ◽  
Hamed Afkhami ◽  
Mansoor Khaledi ◽  
Javad Fathi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Medical Science ◽  
Point Mutations ◽  
Upstream Region ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Base Substitution ◽  
Coding Region ◽  
Biochemical Tests ◽  
Disk Diffusion Method

Abstract Background: The current study was aimed to investigate the prevalence of the mutations of the oprD gene among imipenem-resistant and -sensitive Pseudomonas aeruginosa isolated from educational hospitals in Yazd, Iran.Methods: In this study, 90 P. aeruginosa isolates were collected from different clinical samples and transferred to the Department of Microbiology, Shahid Sadoghi University of Medical Science, during 2015 to 2016. All isolates were identified by the conventional biochemical tests and antibiotic resistance was determined using disk diffusion method. E. test was performed to determine the minimum inhibitory concentrations (MIC) of imipenem. The mutations of upstream of the oprD coding region and its promoters and 54 primary nucleotide of this gene were recognized by the amplification of this region and subsequently sequenced.Results: Seventy (77.7%) of isolates had MIC≥16 and were resistant to imipenem. The results showed that the rate of resistance to imipenem is increasing. Mutations of the upstream of the oprD gene and its promoters were seen in 25 (86.2%) of isolates and 4 strains had no mutation. All of the imipenem-resistant isolates had mutations. One isolate had a base substitution A→ C at nt 25 in coding region and this isolate had a point mutation leading to an amino acid change at positions 9 (I→L). Conclusion: The results showed that imipenem resistance is increasing in P. aeruginosa, also indicated that the point mutations were the most common cause of the inactivation of upstream of the oprD coding region among P. aeruginosa isolates, it seems this mechanism is effective in resistance of isolates to imipenem.

scholarly journals Effects of Nd:Yag Laser on some virulence factor genes of Pseudomonas aeruginosa bacteria

2020 ◽  
Vol 25 (2) ◽  
pp. 86
Author(s):  
Aidah Abd Al-doori1 ◽  
Awatef Saber Jasem1 ◽  
Adnan F. AL-Azzawie2
Keyword(s):  
Pseudomonas Aeruginosa ◽  
General Hospital ◽  
Biological Effects ◽  
Genetic Effect ◽  
Environmental Water ◽  
Clinical Samples ◽  
Genetic Changes ◽  
Yag Laser ◽  
Ear Infections ◽  
Effects Of Radiation

The aim of this study was to assess effects of the 532nm Nd-yag laser on the genes of Tox A, Exo S, and Opr L, of Pseudomonas aeruginosa (P. aeruginosa) bacteria isolated from clinical (wounds, burns, otitis media) and environmental (water, soil) samples. Clinical samples were collected from patients coming to Saladdin General Hospital from wound, burns and middle ear infections while environmental samples were extracted from water and soil for Saladdin General Hospital . Bacterial samples irradiated by Nd-Yag laser with wavelength of 532 nm using energies (300mj,500mj) with (15 and 25 sec) and genomic DNA were extracted from all samples after the diagnosis of P. aeruginosa bacteria depending on the macroscopic and biochemical examination, then the PCR technique was performed. The results have shown an impact on P. aeruginosa bacteria of Nd-Yag laser by comparing PCR results of treated samples with control (unexposed) as loss of normal bands. This indicates that the laser had a genetic effect on the P. aeruginosa bacteria. We conclude that the laser induces genetic changes in P. aeruginosa's DNA so that lasers can be used in treatment and sterilization for clinical and environmental . The PCR technique could be used as a biomarker study to determine the biological effects of radiation on bacteria.   http://dx.doi.org/10.25130/tjps.25.2020.034

Isolation and Diagnosis of Pathogenic Bacteria from the Upper Surface of the Cell Phone Screen and Conduct an Antibiotic Sensitivity Test

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Muthana Badeea Farhan ◽  
Khadija Khleaf Abdulla
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Phone ◽  
Pathogenic Bacteria ◽  
Bacterial Culture ◽  
Culture Media ◽  
Antibiotic Sensitivity ◽  
Personal Communication ◽  
Total Sample ◽  
Final Diagnosis ◽  
Positive Bacterial Culture

A total of 120 samples (80) female samples and 40 male samples were collected from the front of the personal communication (the cell phone) for female students of Education College for Women (65) as well the medical staff of Ramadi Teaching Hospital (55), The samples are collected by swabs, The ages ranged between 19-60 years, These samples were initially culture on the blood agar medium and then on different selective and differential culture media. Api test strips were also used for the final diagnosis of bacterial isolates. The results showed that all samples gave 100% a positive result of general bacterial culture and 80% of pathogenic bacteria. The results of isolating and diagnosism indicated the presence of the pathogenic bacteria Staphylococcus aureus (58.3% of the total sample), Escherichia coli (44.2%), Enterococcus faecalis (25.8%), Pseudomonas aeruginosa (23.3%), Proteus sp. (5%), The results showed that the positive bacterial culture rate for male samples was 87.5%, whereas the female bacterial culture rate was 76.3%. The most important isolates were highly resistant to cephalexin, followed kanamycin. Proteus sp. gave the largest percentage of the resistance to 100% of those antibiotics, except for nitrofurantoin followed by Pseudomonas aeruginosa in the proportion of resistance to antibiotics used in this study.

Sign in / Sign up

Export Citation Format

Share Document