Ethnobotany and species specific molecular markers of some medicinal sakhan (Piper, Piperaceae)

5S rDNA is organized as a cluster of tandemly repeated monomers that consist of the conservative 120 bp coding part and non-transcribed spacers (NTSs) with different lengths and sequences among different species. The polymorphism in the 5S rDNA NTSs of closely related species is interesting for phylogenetic and evolutional investigations, as well as for the development of molecular markers. In this study, the 5S rDNA NTSs were amplified with universal 5S1/5S2 primers in some species of the Elaeagnaceae Adans. family. The polymerase chain reaction (PCR) products of five Elaeagnus species had similar lengths near 310 bp and were different from Shepherdia canadensis (L.) Nutt. and Sh. argentea (Pusch.) Nutt. samples (260 bp and 215 bp, respectively). The PCR products were cloned and sequenced. An analysis of the sequences revealed that intraspecific levels of NTS identity are high (approximately 95–96%) and similar in the Elaeagnus L. species. In Sh. argentea, this level was slightly lower due to the differences in the poly-T region. Moreover, the intergeneric and intervarietal NTS identity levels were studied and compared. Significant differences between species (except E. multiflora Thunb. and E. umbellata Thunb.) and genera were found. Herein, a range of the NTS features is discussed. This study is another step in the investigation of the molecular evolution of Elaeagnaceae and may be useful for the development of species-specific DNA markers in this family.

Download Full-text

Embryo-induced transcriptome changes in bovine endometrium reveal species-specific and common molecular markers of uterine receptivity

Reproduction ◽

10.1530/rep.1.00996 ◽

2006 ◽

Vol 132 (2) ◽

pp. 319-331 ◽

Cited By ~ 146

Author(s):

Stefan Bauersachs ◽

Susanne E Ulbrich ◽

Karin Gross ◽

Susanne E M Schmidt ◽

Heinrich H D Meyer ◽

...

Keyword(s):

Molecular Markers ◽

Molecular Mechanisms ◽

Cdna Array ◽

Early Embryo ◽

Cdna Libraries ◽

Control Group ◽

Regulation Of Transcription ◽

Uterine Receptivity ◽

Subtracted Cdna Libraries ◽

Species Specific

The endometrium plays a central role among the reproductive tissues in the context of early embryo–maternal communication and pregnancy. This study investigated transcriptome profiles of endometrium samples from day 18 pregnant vs non-pregnant heifers to get insight into the molecular mechanisms involved in conditioning the endometrium for embryo attachment and implantation. Using a combination of subtracted cDNA libraries and cDNA array hybridisation, 109 mRNAs with at least twofold higher abundance in endometrium of pregnant animals and 70 mRNAs with higher levels in the control group were identified. Among the mRNAs with higher abundance in pregnant animals, at least 41 are already described as induced by interferons. In addition, transcript levels of many new candidate genes involved in the regulation of transcription, cell adhesion, modulation of the maternal immune system and endometrial remodelling were found to be increased. The different expression level was confirmed with real-time PCR for nine genes. Localisation of mRNA expression in the endometrium was shown byin situhybridisation forAGRN,LGALS3BP,LGALS9,USP18,PARP12andBST2. A comparison with similar studies in humans, mice, and revealed species-specific and common molecular markers of uterine receptivity.

Download Full-text

The Development of Populus alba L. and Populus tremula L. Species Specific Molecular Markers Based on 5S rDNA Non-Transcribed Spacer Polymorphism

Forests ◽

10.3390/f10121092 ◽

2019 ◽

Vol 10 (12) ◽

pp. 1092 ◽

Cited By ~ 1

Author(s):

Alexandrov ◽

Karlov

Keyword(s):

Molecular Markers ◽

Tree Species ◽

5S Rdna ◽

Populus Tremula ◽

Plant Science ◽

Populus Alba ◽

Natural Hybrid ◽

Species Specific ◽

Genetics Of Populations

The Populus L. genus includes tree species that are botanically grouped into several sections. This species successfully hybridizes both in the same section and among other sections. Poplar hybridization widely occurs in nature and in variety breeding. Therefore, the development of poplar species’ specific molecular markers is very important. The effective markers for trees of the Aigeiros Duby section have recently been developed using the polymorphism of 5S rDNA non-transcribed spacers (NTSs). In this article, 5S rDNA NTS-based markers were designed for several species of the Leuce Duby section. The alb9 marker amplifies one fragment with the DNA matrix of P. alba and P. × canescens (natural hybrid P. alba × P. tremula). The alb2 marker works the same way, except for the case with Populus bolleana. In this case, the amplification of three fragments was observed. The tremu1 marker amplification was detected with the DNA matrix of P. tremula and P. × canescens. Thus, the developed markers may be applied as a useful tool for P. alba, P. tremula, P. × canescens, and P. bolleana identification in various areas of plant science such as botany, dendrology, genetics of populations, variety breeding, etc.

Download Full-text

The Development of New Species-Specific Molecular Markers Based on 5S rDNA in Elaeagnus L. Species

Plants ◽

10.3390/plants10122713 ◽

2021 ◽

Vol 10 (12) ◽

pp. 2713

Author(s):

Oleg S. Alexandrov ◽

Gennady I. Karlov

Keyword(s):

Molecular Markers ◽

New Species ◽

Western Europe ◽

Economic Value ◽

Ornamental Plants ◽

Far East ◽

Genetic Research ◽

5S Rdna ◽

Identification Of Species ◽

Species Specific

The Elaeagnus L. species are trees and bushes that mainly grow in temperate zones of Western Europe; Minor, Central, and Southeast Asia; the Far East; and North America. Some species are used as fruit or ornamental plants and have economic value. Problems with the identification of species in the Elaeagnus genus by molecular genetical methods arise in the study of populations, systematics, breeding, and other areas of plant science and practice. Recently, the polymorphism of 5S ribosomal DNA non-transcribed spacers (5S rDNA NTSs) in Elaeagnaceae Adans. has been described. The results were used in our study as a basis for development of new species-specific molecular markers for some members of the Elaeagnus genus. The author’s method was applied for finding regions that were potentially applicable for species-specific primer design. As a result, some species-specific molecular markers were developed for Elaeagnus angustifolia L., E. commutata Bernh., E. pungens Thunb., and E. multiflora Thunb. These markers were tested in a range of samples and showed the presence of amplified fragments in lanes of the marked species only. Samples of other species showed no amplifications. Thus, the developed markers may be useful for the species identification of the studied Elaeagnus plants in botanical, dendrological, and genetic research (especially in a leafless period of year), as well as in breeding and hybridization experiments.

Download Full-text

Termite Taxonomy, Challenges and Prospects: West Africa, A Case Example

Insects ◽

10.3390/insects10010032 ◽

2019 ◽

Vol 10 (1) ◽

pp. 32 ◽

Cited By ~ 6

Author(s):

Judith Korb ◽

Boris Kasseney ◽

Yvonne Cakpo ◽

Robin Casalla Daza ◽

Jean Gbenyedji ◽

...

Keyword(s):

Molecular Markers ◽

Species Delimitation ◽

Morphological Traits ◽

Global Scale ◽

West African ◽

Species Level ◽

Ecological Research ◽

Species Specific ◽

Structured Approach

Termites are important ecosystem engineers. Yet they are often difficult to identify due to the lack of reliable species-specific morphological traits for many species, which hampers ecological research. Recently, termitologists working with West African termites (West African Termite Taxonomy Initiative) convened for a workshop with the aim of beginning to address this problem. Repeated determination of the same termite samples by the most renowned taxonomists for West African termites identified the huge scale of the problem, as less than 10% of all species could be unambiguously determined to the species level. Intensive discussions and comparisons increased the identification success to around 25% at the end of the workshop. Yet many groups remained problematic and molecular markers and barcoding techniques combined with species delimitation approaches will be needed to help resolve these existing taxonomic problems. Based on the outcome of this workshop, we propose concerted initiatives to address termite taxonomy on a global scale. We are convinced that dedicated workshops on regional taxonomy that follow a similar structured approach, with repeated determination of the same sample, will help overcome the difficulties that termite taxonomy faces. This initiative can also serve as a blueprint for other taxonomical groups that are difficult to identify.

Download Full-text

Phlebotomus caucasicus and Phlebotomus mongolensis (Diptera: Psychodidae): indistinguishable by the mitochondrial cytochrome b gene in Iran

Bulletin of Entomological Research ◽

10.1017/s0007485309990423 ◽

2009 ◽

Vol 100 (4) ◽

pp. 415-420 ◽

Cited By ~ 20

Author(s):

P. Parvizi ◽

H. Taherkhani ◽

P.D. Ready

Keyword(s):

Molecular Markers ◽

Cytochrome B ◽

External Genitalia ◽

Morphological Characters ◽

Gene Fragment ◽

Lineage Sorting ◽

Zoonotic Cutaneous Leishmaniasis ◽

Phlebotomine Sandflies ◽

Mitochondrial Cytochrome B ◽

Species Specific

AbstractDiagnostic molecular markers for the females of Phlebotomus (Paraphlebotomus) caucasicus and P. mongolensis were sought by characterizing from individual Iranian specimens a gene fragment, namely mitochondrial cytochrome b, that had previously proven useful for the taxonomy of phlebotomine sandflies. Males of both species were used as reference material because their external genitalia provide the only diagnostic morphological characters. A phylogenetic analysis of the new sequences, and those previously reported for P. grimmi, found no support for recognizing more than one species (P. caucasicus s.l.) in Iran. Most of the genetic variation was geographical. An absence of lineage sorting was demonstrated, and it is proposed that any search for species-specific molecular markers for these three taxonomic species should be continued by applied biologists only if there is better evidence for associating any one of them with phenotypes important for understanding the transmission of Leishmania species in foci of zoonotic cutaneous leishmaniasis.

Download Full-text

Development of species-specific molecular markers in Vanilla for seedling selection of hybrids

Molecular Biology Reports ◽

10.1007/s11033-020-05287-9 ◽

2020 ◽

Vol 47 (3) ◽

pp. 1905-1920

Author(s):

Juan Li ◽

Lynhe Demesyeux ◽

Maria Brym ◽

Alan H. Chambers

Keyword(s):

Molecular Markers ◽

Species Specific ◽

Selection Of

Download Full-text

Development of 5S rDNA-Based Molecular Markers for the Identification of Populus deltoides Bartr. ex Marshall, Populus nigra L., and Their Hybrids

Forests ◽

10.3390/f9100604 ◽

2018 ◽

Vol 9 (10) ◽

pp. 604 ◽

Cited By ~ 4

Author(s):

Oleg Alexandrov ◽

Gennady Karlov

Keyword(s):

Molecular Markers ◽

Species Identification ◽

Populus Deltoides ◽

5S Rdna ◽

High Ability ◽

Hybrid Analysis ◽

Marker System ◽

Interspecies Hybridization ◽

Poplar Breeding ◽

Species Specific

Populus L. is a tree genus that includes species with a high ability for interspecies hybridization. This process takes place in nature, and is used in poplar breeding. As а result, species identification in poplar populations and plantations is very difficult. In this study, a molecular marker system was developed for the identification of the most significant poplar species (P. nigra L. and P. deltoids Bartr. ex Marshall). The basis of the system is a polymorphism in non-transcribed spacers (NTSs) of 5S rDNA. The species-specific molecular markers were tested on a number of species and hybrids of poplars. It was shown that the marker system is a powerful tool for species identification, hybrid analysis, parent identification, and poplar breeding.

Download Full-text

Molecular markers for Peristenus spp. (Hymenoptera: Braconidae) parasitoids associated with Lygus spp. (Hemiptera: Miridae)

The Canadian Entomologist ◽

10.4039/n02-017 ◽

2003 ◽

Vol 135 (1) ◽

pp. 71-83 ◽

Cited By ~ 25

Author(s):

M. Erlandson ◽

L. Braun ◽

D. Baldwin ◽

J. Soroka ◽

M. Ashfaq ◽

...

Keyword(s):

Molecular Markers ◽

Dna Sequence ◽

Sequence Data ◽

Pcr Primers ◽

Species Level ◽

Rrna Genes ◽

Specific Primers ◽

Canadian Prairies ◽

Dna Sequence Data ◽

Species Specific

AbstractMolecular markers for identifying Peristenus spp. parasitoids to species level and preliminary molecular markers to distinguish two groups of Lygus spp. common to the Canadian prairies were developed. Peristenus species-specific polymerase chain reaction (PCR) primers were developed based on DNA sequence data from a 1600-bp region of the internal transcribed spacer region between the 5.8S and 18S nuclear rRNA genes (ITS2). These primers were able to distinguish Peristenus digoneutis Loan, Peristenus stygicus Loan, and Peristenus pallipes (Curtis). Their ability to identify to species-level parasites dissected from field-collected Lygus spp. nymphs was examined by analysis of DNA from 100 parasite samples. Of those samples showing positive PCR amplification with both control (ITS2) and species-specific primers, all were positive for P. pallipes; none of the samples amplified appropriately sized products with P. digoneutis specific or P. stygicus specific primers. These findings were validated using restriction enzyme digests of amplified regions of the Peristenus spp. cytochrome oxidase 1 gene. Both methods were consistent with earlier studies that showed P. pallipes to be the only species of the genus Peristenus to be associated with Lygus spp. on the Canadian prairies. PCR primers based on DNA sequence data from a 550-bp region of the mitochondrial 16S rRNA gene were designed to discriminate Lyguslineolaris (Palisot de Beauvois) from Lygus borealis (Kelton), and Lygus elisus (Van Duzee). These PCR primers were used to identify field-collected nymphs, with most being identified as either L. borealis/L. elisus (72–82%) orL. lineolaris (14–18%). These estimates of species composition closely reflected those of subsequent adult population surveys from the same fields.

Download Full-text