Development of an Environmentally Friendly Larvicidal Formulation Based on Essential Oil Compound Blend to Control Aedes aegypti Larvae: Correlations between Physicochemical Properties and Insecticidal Activity

Due to the increasing rate of morbidity and mortality caused by vector diseases in the current context, especially by Aedes aegypti, substances of plants have been studied as alternatives to chemical insecticides, among them, the essential oil (EO) of the species Cymbopogon winterianus. Thus, this study evaluated the larvicidal activity of the EO of C. winterianus against the A. aegypti larvae. EO was extracted through the hydro-distillation technique and physicochemical properties were determined. To evaluate larvicidal activity, tests were performed with larvae in the third instar at the final concentrations of 19.54; 26.50; 55.59; 138.98; 208.47 and 277.97 mg L-1 of C. winterianus EO. In addition, Artemia salina Leach bioassay was used to verify toxicity effect. EO obtained presented satisfactory results in 2.64%. In the larvicidal assay, 100% mortality of larvae was observed after 24 hours at concentrations of 208.47 mg L-1 and 277.97 mg L-1 of the EO, showed effective in the other concentrations and with LC50 of 46.18 mg L-1, considered highly active. In the toxicity assay, the EO presented LC50 at 532.34 mg L-1, showed considered nontoxic. These results reinforce the use of EO front A. aegypt larvae control.

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Insecticidal and Attractant Activities of Magnolia citrata Leaf Essential Oil against Two Major Pests from Diptera: Aedes aegypti (Culicidae) and Ceratitis capitata (Tephritidae)

Molecules ◽

10.3390/molecules26082311 ◽

2021 ◽

Vol 26 (8) ◽

pp. 2311

Author(s):

Ngoc Anh Luu-Dam ◽

Nurhayat Tabanca ◽

Alden S. Estep ◽

Duy Hung Nguyen ◽

Paul E. Kendra

Keyword(s):

Puerto Rico ◽

Essential Oil ◽

Aedes Aegypti ◽

Insecticidal Activity ◽

Ceratitis Capitata ◽

Fruit Fly ◽

Positive Control ◽

Sterile Male ◽

Leaf Essential Oil ◽

First Time

In this study, Magnolia citrata Noot and Chalermglin (Magnoliaceae) essential oil (MCEO) was evaluated for insecticidal activity against the yellow fever mosquito Aedes aegypti and attractant activity for the Mediterranean fruit fly Ceratitis capitata. The leaves of Magnolia citrata (Giổi chanh) were collected from northwestern Vietnam, and the water-distilled MCEO was analyzed by gas-chromatography and mass spectrometry (GC-MS). The major constituents of MCEO were identified as linalool 19%, geranial 16%, citronellal 14%, neral 14%, and sabinene 12%. MCEO showed 100% mortality at 1 μg/μL against 1st instar larvae of Ae. aegypti (Orlando strain, ORL), and the oil exhibited 54% (ORL) and 68% (Puerto Rico strain) mortality at 5 μg/mosquito against Ae. aegypti adult females. Initial screens showed that MCEO had weak insecticidal activity compared to the positive control permethrin. In bioassays with sterile male C. capitata, MCEO exhibited moderately strong attraction, comparable to that observed with a positive control, Tetradenia riparia essential oil (TREO). Herein, the insecticidal and attractant activities of MCEO are reported for the first time.

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ACTIVITY LARVICIDE OF THE ESSENTIAL OIL OF CINNAMOMUM ZEYLANICUM BLUME

Periódico Tchê Química ◽

10.52571/ptq.v16.n31.2019.24_periodico31_pgs_18_26.pdf ◽

2019 ◽

Vol 16 (31) ◽

pp. 18-26

Author(s):

Paulo Roberto Barros GOMES ◽

Dayara Pereira SANTOS ◽

Victor Elias Mouchrek FILHO ◽

Leandra Sofia dos Santos MENDES ◽

Maria Alves FONTENELE

Keyword(s):

Gas Chromatography ◽

Refractive Index ◽

Essential Oil ◽

Aedes Aegypti ◽

Physicochemical Properties ◽

Biological Properties ◽

The Other ◽

Spectroscopic Techniques ◽

Cinnamomum Zeylanicum ◽

Oil Density

The genus Cinnamomum (Lauraceae) consists of approximately 350 species, many of which are essential oil producers, with the most frequently used parts of the plants being dehydrated bark. Therefore, Cinnamomum zeylanicum Blume, known popularly as cinnamon, has the following biological properties: analgesic, antiseptic, antispasmodic, astringent, insecticidal, antifungal and antimicrobial. Thus, this work evaluates the physicochemical properties and larvicidal activity against Aedes aegypti of the essential oil of Cinnamomum zeylanicum Blume leaves that are found in the Municipality of São Luís, Maranhão. The essential oil was quantitatively extracted by hydrodistillation. The physicochemical properties of the essential oil (density, refractive index, solubility, color, and appearance) were determined. The oil was characterized analytically by gas chromatography coupled to mass spectrometer (GC-MS) and the CL50 of the oil was calculated from the Reed-Muench Pizzi methods, respectively. The yield of the oil was 4.33% of mass per volume, which allowed the identification of 4 components, being the major presence of eugenol that soon was confirmed by the spectroscopic techniques. The essential oil obtained CL50 of 56.49 mg∙L-1. In the identification of the major component and the other components, as well as their quantifications, the techniques were precise and the methods efficient, providing a good analytical performance in the determinations.

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Chemical composition of Paeonia anomala essential oil and its insecticidal activity against Aedes aegypti

Planta Medica ◽

10.1055/s-0035-1545176 ◽

2015 ◽

Vol 81 (05) ◽

Author(s):

N Tabanca ◽

A Ali ◽

UR Bernier ◽

G Ozek ◽

T Ozek ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Insecticidal Activity

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Insecticidal activity of essential oil from Chamaecyparis formosensis Matsum

Holzforschung ◽

10.1515/hf.2007.087 ◽

2007 ◽

Vol 61 (5) ◽

pp. 595-599 ◽

Cited By ~ 17

Author(s):

Pei-Min Kuo ◽

Fang-Hua Chu ◽

Shang-Tzen Chang ◽

Wen-Feng Hsiao ◽

Sheng-Yang Wang

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Aedes Albopictus ◽

Insecticidal Activity ◽

Tree Species ◽

Raw Material ◽

Mosquito Larvae ◽

Chamaecyparis Formosensis ◽

Insecticidal Activities

Abstract Chamaecyparis formosensis is an endemic precious tree species grown in Taiwan. Based on the results obtained in our current study, the essential oil of C. formosensis wood possesses significant insecticidal activities. In an assay to assess mosquito larvicidal properties, the essential oil completely killed the larvae of Aedes aegypti and Aedes albopictus at a dosage of 100 μg ml-1 in water. Concentrations of the essential oil at a dosage of 0.16 mg cm-3 in air resulted in 100% mortality of silverfish after 2 h. It was found that the extractives of C. formosensis possess multiple bioactivities against insects. There is great potential for the development of insecticides for domestic application to kill mosquito larvae and silverfish based on this raw material.

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Physicochemical Characterization and Antinociceptive Effect of β-cyclodextrin/Lippia pedunculosa Essential Oil in Mice

Current Topics in Medicinal Chemistry ◽

10.2174/1568026618666180607081742 ◽

2018 ◽

Vol 18 (9) ◽

pp. 797-807 ◽

Cited By ~ 1

Author(s):

Paula dos Passos Menezes ◽

Francielly de Oliveira Araujo ◽

Tatianny Araujo Andrade ◽

Igor Araujo Santos Trindade ◽

Heitor Gomes de Araujo-Filho ◽

...

Keyword(s):

Essential Oil ◽

Physicochemical Properties ◽

Water Solubility ◽

Therapeutic Potential ◽

Biological Activities ◽

Antinociceptive Effect ◽

Physicochemical Characterization ◽

Good Tool ◽

Main Challenge ◽

Higher Temperature

Background: Some research studies have shown that Lippia pedunculosa essential oil (EOLP) has interesting biological activities. However, its low water solubility is the main challenge to achieve its therapeutic potential. In this context, Cyclodextrins (CDs) have been widely used in order to overcome this problem due to your capability to improve the physicochemical properties of drugs. Objective: In this perspective, the main goal of this study was to investigate how the improvement of the physicochemical properties of inclusion complexes (EOLP and β-CD) enhance the antinociceptive effect in mice. Methods: To achieve that, we prepared samples by Physical Mixture (PM), Paste Complexation (PC) and Slurry Complexation (SC) methods, followed by their physicochemical characterization. In addition, it was evaluated if the use of β-CD enhances the antinociceptive effect of EOLP in mice. Results: The analysis showed that rotundifolone (72.02%) was the major compound of EOLP and we found out based on DSC results that β-CD protected it from oxidation. In addition, TG techniques demonstrated that the best inclusion methods were PC and SC, due to their greater weight loss (10.8 and 11.6%, respectively) in the second stage (171-312°C), indicating that more complexed oil was released at the higher temperature than oil free. Other characteristics, such as changes in the typical crystalline form, and reduced particle size were observed by SEM and laser diffraction, respectively. The SC was the most effective complexation method, once the presence of rotundifolone was detected by FTIR. Based on that, SC method was used in all mice tests. In this regard, the number of paw licks was reduced for both compounds (all doses), but EOLP was more effective in reducing the nociceptive behavior. Conclusion: Therefore, CDs seem not to be a good tool to enhance the pharmacological properties of EOs rich in peroxide compounds such as rotundifolone.

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