Repellent and Larvicidal Activity of Ruta graveolens Essential Oil and its Major Individual Constituents Against Aedes aegypti

Aedes aegypti is a species of mosquito with wide distribution worldwide, involved in the cycles of transmission of dengue, Zika and chikungunya. The lack of vaccines for most of these arboviruses highlights the importance of vector control as a measure to reduce the incidence of these diseases. Several researches have been carried out in order to find substances of plant origin that are candidates for the control of A. aegypti. In this sense, medicinal plants that also have an insecticidal action have shown promise for the development of new products, environmentally safer than traditional chemical insecticides. The objective of this work was to evaluate the larvicidal activity of the essential oil of the leaves of Croton rhamnifolioides and its formulation in nanocapsules for A. aegypti. The essential oil was extracted by hydrodistillation and analyzed by gas chromatography coupled to a mass spectrometer (CG/MS). Formulations were produced containing concentrations of pure oil, which ranged from 40 to 80 μg/mL and polycaprolactone nanocapsules with concentrations from 30 to 120 μg/mL. Larvicide tests were performed using varying concentrations of an aqueous suspension, in triplicates, containing 20 larvae / concentration, in addition to an untreated control. As a result, the essential oil had an average content that varied between 1.4% and 0.58%, depending on the time of collection and, as main constituents, eucalyptol (16.57%) and (E) -cariophylene (11, 32%). LC50 values ranged from 26.3 μg/mL to 52.2 μg/mL and CL90 from 37.8 μg/mL to 68.7 μg/mL for pure oil. In encapsulated oil, the LC50 = 63.4 μg/mL and the LC90 = 104.8 μg/mL. The pure oil of Croton rhamnifolioides was considered more active against the larvae of A. aegypti than the encapsulated one, although both have great potential for application as a larvicide.

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Composition and Larvicidal Activity of Artemisia Vulgaris L. Stem Essential Oil against Aedes Aegypti

Jordan Journal of Biological Sciences ◽

10.12816/0000252 ◽

2013 ◽

Vol 6 (1) ◽

pp. 11-16 ◽

Cited By ~ 9

Author(s):

Sujatha Govindaraj ◽

Bollipo D. Ranjitha Kumari

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Artemisia Vulgaris

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Effect of stalk and leaf extracts from Euphorbiaceae species on Aedes aegypti (Diptera, Culicidae) larvae

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652006000400007 ◽

2006 ◽

Vol 48 (4) ◽

pp. 211-214 ◽

Cited By ~ 24

Author(s):

Maria Goretti Araújo de Lima ◽

Ismália Cassandra Costa Maia ◽

Bruna Dantas de Sousa ◽

Selene Maia de Morais ◽

Sílvia Maria Freitas

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Aqueous Solutions ◽

Larvicidal Activity ◽

Steam Distillation ◽

Variance Analysis ◽

Leaf Extracts ◽

Plant Parts ◽

Tukey Test ◽

Different Parts

The objective of this study was to evaluate the larvicidal activity of essential oil aqueous solutions (hydrolates) obtained by steam distillation of stalks and leaves of Croton argyrophylloides, Croton nepetaefolius, Croton sonderianus and Croton zehntneri against Aedes aegypti larvae. Twenty-five larvae of third instar were placed in plastic beckers, containing the hydrolates (50 mL), in a four repetitions scheme. Water was used as control and the number of dead larvae was counted after 24 hours. The data obtained were submitted to Variance Analysis and Tukey test. Significant differences were observed among the hydrolates from different species and from different parts of each plant (p < 0.001). The hydrolates of stalk and leaf from C. nepetaefolius and C. zehntneri and leaf hydrolate of C. argyrophylloides presented 100% mortality against larvae. The compounds present in C. zenhtneri and C. nepetaefolius are oxygenated phenylpropanoids that are more soluble in water than the monoterpenes and sesquiterpenes detected in the oils of C. argyrophylloides and C. sonderianus. This study showed that all species analyzed presented compounds with larvicidal properties, with differences between each plant parts.

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Chemical Composition and Larvicidal Activity of Eugenia triquetra Essential Oil from Venezuelan Andes

Natural Product Communications ◽

10.1177/1934578x1000500633 ◽

2010 ◽

Vol 5 (6) ◽

pp. 1934578X1000500 ◽

Cited By ~ 1

Author(s):

Flor D. Mora ◽

Jorge L. Avila ◽

Luis B. Rojas ◽

Rosslyn Ramírez ◽

Alfredo Usubillaga ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Chemical Constituents ◽

Ms Analysis ◽

Third Instar Larvae

The chemical constituents of the essential oil obtained by hydrodistillation of the leaves of Eugenia triquetra O. Berg, collected in Táchira State, Venezuela, were identified by GC-MS analysis. Twenty-six components, which made up 88.5% of the oil, were identified. The major constituents were linalool (17.5%), limonene (16.9%), α-pinene (11.6%), β-pinene (8.7%), and p-cymene (3.7%). The essential oil was tested against third-instar larvae of Aedes aegypti, showing a LC50 value of 64.8 ± 5.6 ppm.

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Larvicidal activity of the essential oil from Lippia sidoides cham. against Aedes aegypti linn

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762003000400027 ◽

2003 ◽

Vol 98 (4) ◽

pp. 569-571 ◽

Cited By ~ 102

Author(s):

Ana Fontenele Urano Carvalho ◽

Vânia Maria Maciel Melo ◽

Afrânio Aragão Craveiro ◽

Maria Iracema Lacerda Machado ◽

Márcia Braga Bantim ◽

...

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Lippia Sidoides

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Larvicidal Activity of Essential Oils from the Leaves and Fruits of Nutmeg (Myristica fragrans Houtt) Against Aedes aegypti (Diptera: Culicidae)

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v4i7.552-556.705 ◽

2016 ◽

Vol 4 (7) ◽

pp. 552

Author(s):

Anne Carolina ◽

Maman Maman

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Steam Distillation ◽

Chemical Compounds ◽

Chemical Components ◽

Myristica Fragrans ◽

Leaf Oil ◽

Myristica Fragrans Houtt ◽

Fruit Oil

The aim of this research was to examine the larvicidal activity of essential oil (EO) extracted from nutmeg (Myristica fragrans Houtt) leaves and fruits by steam distillation, and to analyze its chemical compounds. The EO yield of nutmeg leaves and fruits collected from the same tree was 0.66% and 0.30%, respectively. Larvicidal tests with the EO were carried out against Aedes aegypti (L.) (Diptera: Culicidae). The concentrations of nutmeg EO used for the larvicidal assay were 50, 100, 150, 200, and 250 μg/mL. The results showed that fruit oil was more toxic than the leaf oil. LC50 values of leaf and fruit EOs were 133.8 and 110.1 µg/mL, respectively. The chromatogram of GC-MS showed that the chemical components in nutmeg leaf and fruit EOs were dominated by α-pinene, sabinene, β-pinene, delta-3-carene, limonene, β-phellandrene, α-terpinolene, linalool, safrole, croweacin, and myristicin.

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Larvicidal activity of microparticles of Melissa officinalis L. essential oil (Lamiaceae) against Aedes aegypti (Diptera, Culicidae)

Research Society and Development ◽

10.33448/rsd-v10i1.11166 ◽

2021 ◽

Vol 10 (1) ◽

pp. e35710111166

Author(s):

Thércia Gabrielle Teixeira Martins ◽

Paulo Victor Serra Rosa ◽

Mariana Oliveira Arruda ◽

Andressa Almeida Santana Dias ◽

Ari Pereira de Araújo Neto ◽

...

Keyword(s):

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Fourth Instar ◽

Tween 20 ◽

Distilled Water ◽

Melissa Officinalis ◽

The Face ◽

Muench Method

This study aimed to evaluate the larvicidal activity of essential oil microparticles (EO) of Melissa officinalis L. against Aedes aegypti. The leaves of M. officinalis were collected in the municipality of São José de Ribamar, Maranhão, Brazil, later dried, crushed and ground. 90g of the dried leaves were used to obtain the EO by the hydrodistillation method. For the synthesis of microencapsulated EO, 60g of sodium alginate (2.5% m/v) was added to the mixture of 15g of Tween 20 with 6g of EO. The mixture was homogenized and drips over CaCl2 5% m/v solution for the hardening of particles via crosslinking. The microparticles were washed with distilled water in filter and dried at 35ºC/24h and 15 days at tamb (30ºC). The eggs of Aedes aegypti were collected at the Federal University of Maranhão by the ovitrampas method. The larvae that hatched were fed until they reached the fourth instar. Groups of larvae (n=20) were submitted to solutions of EO and microparticles of 10-90 mg/L . After 24 h, live and dead larvae were counted and LC50 was calculated by the Reed&Muench method, using Cheng's criterion for classification of active potential. All larvae presented mortality in all concentrations tested. The LC50 obtained for the EO was 40.60 mg/L and for the microparticles 22.10 mg/L, both classified as active according to the adopted criterion, but it is observed that the microparticles increased the larvicidal potential of the EO. Through the results obtained, it is concluded that the microparticles formulated with the EO proved to be efficient in the face of the larvae of Aedes aegypti, being interesting and important in controlling and combating the mosquito that transmits dengue.

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Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

10.1101/595900 ◽

2019 ◽

Cited By ~ 1

Author(s):

Renata do Socorro Barbosa Chaves ◽

Rosany Lopes Martins ◽

Alex Bruno Lobato Rodrigues ◽

Érica de Menezes Rabelo ◽

Ana Luzia Ferreira Farias ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Aedes Aegypti ◽

Larvicidal Activity ◽

Artemia Salina ◽

High Cytotoxic Activity ◽

Origanum Majorana ◽

Main Component

ABSTRACTThis study evaluated the larvicidal activity of O. majorana essential oil, identified the chemical composition, evaluated the antimicrobial, cytotoxic and antioxidant potential. The larvicidal activity was evaluated against larvae of the third stage of Aedes aegypti, whereas the chemical composition was identified by gas chromatography coupled to mass spectrometer, the antimicrobial activity was carried out against the bacteria Pseudomonas aeruginosa, Escherichia coli and Staphylococcus auereus, the antioxidant activity was evaluated from of 2.2-diphenyl-1-picryl-hydrazila sequestration and Artemia salina cytotoxicity. Regarding to the results, the larvicidal activity showed that O. majorana essential oil caused high mortality in A. aegypti larvae. In the chromatographic analysis, the main component found in O. majorana essential oil was pulegone (57.05%), followed by the other components verbenone (16.92%), trans-p-menthan-2-one (8.57%), iso-menthone (5.58%), piperitone (2.83%), 3-octanol (2.35%) and isopulegol (1.47%). The antimicrobial activity showed that E. coli and P. aeruginosa bacteria were more sensitive to oil than S. aureus, which was resistant at all concentrations. Essential oil did not present antioxidant activity, but it has high cytotoxic activity against A. salina.

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