Amelioration of Streptozotocin induced hyperglycemia in rats using combination of Gymnema sylvestre and Eugenia jambolana plant extracts

Background: Diabetes Mellitus is a chronic disorder characterised by abnormally elevated glucose levels in the blood. Diabetes is caused by one of two mechanisms: insufficient insulin synthesis (which is produced by the pancreas and reduces blood glucose) or insufficient response of cells to insulin action. The current aim of this research project was to formulate and evaluate the Polyherbal preparation (PHP) of the plants constituted with Cinnamonum zeylanicium (CJ) bark, Eugenia jambolana (EJ) seeds, Vinca rosea (VR) whole plant, Gymnema sylvestre (GS) leaves and determination of the anti-diabetic potential of the formulation in the animal model induced by Streptozotocin. Methods: Plant components in the current study used were Cinnamonum zeylanicium (CJ) bark, Eugenia jambolana (EJ) seeds, Vinca rosea (VR) whole plant, Gymnema sylvestre (GS) leaves were collected.Using a hydroalcoholic solvent, physico-chemical parameters and active chemical constituents were evaluated. The active components present in the extracts were identified by Preliminary phytochemical screening. The PHP acute toxicity analysis was conducted in compliance with OECD Guideline 423, with 200 mg/kg and 4000 mg/kg administered orally to rats over 28 days. Results: Diabetes was induced by STZ and treated with PHF did not show any alterations in behavior and no mortality was observed up to the 2000 mg/kg dose level during the interventional period. By oral administration of PHP with a dosage of 200 and 400 mg/kg, OGTT resulted in a steady decrease in blood glucose levels of 68.74±4.63 mg/dl and 63.83±1.74 mg/dl at 180min after the trial which proves that PHP possess anti-diabetic activity. By mixing each extract in varying proportions, PHP was developed and evaluated. PHP (200 and 400mg/kg) antidiabetic activity wasdetermined for streptozotocin (STZ)-induced diabetes in rats and glibenclamide (5.0mg/kg body weight) was used as a standard drug.The investigational drug was administered for 28 days and the blood glucose level effect of the PHP was analysed on the 28th day after the intervention time. Conclusion: The experimental study showed that a persistent and substantial decrease in the average blood glucose level of diabetic rats was observed with repeated administration of PHP and glibenclamide for 28 days. PHP demonstrated substantial antidiabetic and antihyperlipidemic activity similar to the standard drug. The formulation will emerge as a possible mixture that may challenge the synthetic drug.

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In vitro hypoglycemic effects of Gymnema sylvestre, Tinospora cordifolia, Eugenia jambolana and Aegle marmelos

Journal of Natural Pharmaceuticals ◽

10.4103/2229-5119.83950 ◽

2011 ◽

Vol 2 (2) ◽

pp. 52 ◽

Cited By ~ 7

Author(s):

Faiyaz Ahmed ◽

NS Siddaraju ◽

Asna Urooj

Keyword(s):

Tinospora Cordifolia ◽

Gymnema Sylvestre ◽

Aegle Marmelos ◽

Eugenia Jambolana

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Complementary and Comparative Study on Hypoglycemic and Antihyperglycemic Activity of Various Extracts of Eugenia jambolana Seed, Momordica charantia Fruits, Gymnema sylvestre, and Trigonella foenum graecum Seeds in Rats

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-009-8799-1 ◽

2009 ◽

Vol 160 (8) ◽

pp. 2388-2400 ◽

Cited By ~ 33

Author(s):

Mukesh Yadav ◽

Amita Lavania ◽

Radha Tomar ◽

G. B. K. S. Prasad ◽

Shalini Jain ◽

...

Keyword(s):

Comparative Study ◽

Momordica Charantia ◽

Gymnema Sylvestre ◽

Antihyperglycemic Activity ◽

Trigonella Foenum Graecum ◽

Eugenia Jambolana

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Anticancer activity of Eugenia jambolana seeds against Hep2 cell lines

The Journal of Phytopharmacology ◽

10.31254/phyto.2015.4604 ◽

2016 ◽

Vol 4 (6) ◽

pp. 295-298

Author(s):

Denis Mabeya Ogato ◽

◽

Eliakim Mbaka Mauti ◽

Godfrey Omare Mauti ◽

Barasa Ambrose ◽

...

Keyword(s):

Anticancer Activity ◽

Plant Extracts ◽

Extraction Method ◽

Active Components ◽

Cell Control ◽

Anticancer Compounds ◽

Hep2 Cell ◽

Eugenia Jambolana ◽

Hot Air ◽

Life Threatening

Cancer is a life-threatening disease and leads to high rates of mortality worldwide, after cardiovascular disease, is the second leading cause of death. Investigations for finding new plant based anticancer compounds are imperative and interesting. There are many studies on anticancer herb/plant extracts in cell line models. Eugenia jambolana has been reported to contain phytochemicals like coumarin, flavanoids, glycosides, phenols, tannins and steroids. The various part of Eugenia jambolana have therapeutic applications. Plant active components were extracted using the decoction extraction method and the filtrate was obtained by means of filtering through a Whattman no.1 filter paper. The filtrate was evaporated in a weighed flask in a hot air oven set at 50°C. Extracts were reconstituted by re-dissolving in respective solvents. Different concentration i.e. 8, 15.6, 31.25, 62.5, 125, 250, 500 and 1000 µg. of the plant extracts were tested for the anticancer activity. The anticancer assay was performed on Human laryngeal epithiloma cells (Hep 2) obtained from King Institute of Preventive Medicine, Chennai, India. The cell viability was measured using MTT assay. Controls were maintained throughout the experiment (Untreated wells as cell control and diluent treated wells as diluent control). The assay was performed in triplicates for each of the extracts.

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