Extraction and Isolation of bioactive compounds from Lantana camara leaves by column Chromatographic techniques

2021 ◽  
Vol 14 (3) ◽  
pp. 1607-1611
Author(s):  
Poongothai Annadurai
Keyword(s):  
Bioactive Compounds ◽  
Lantana Camara ◽  
Chromatographic Techniques ◽  
Column Chromatographic

Phytochemical screening for identification of bioactive compounds of Lantana camara Linn. (Verbenaceae) responsible for larvicidal action against Aedes triseriatus Say, 1823 (Diptera : Culicidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 117-126
Author(s):  
Rhitayu Chakraborti ◽  
Probir Kumar Bandyopadhyay
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Active Ingredient ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Mosquito Larvae ◽  
Aedes Triseriatus ◽  
Phytochemical Screening ◽  
Lc50 Value

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

Hydroxyethyl Isoflavonoids from the Leaves of Maclura tricuspidata

2021 ◽  
Vol 25 ◽  
Author(s):  
Yang Hee Jo ◽  
Sang Won Yeon ◽  
Se Hwan Ryu ◽  
Solip Lee ◽  
Seon Beom Kim ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
2D Nmr ◽  
Chromatographic Techniques ◽  
Glucosidase Activity ◽  
New Compounds

: Maclura tricuspidata, which belongs to the Moraceae family, is widely distributed in Asia, including Korea. All parts of this plant, such as roots, fruits, and leaves, are rich in diverse bioactive compounds, especially xanthones and isoflavonoids. Investigation of the leaves of M. tricuspidata through various chromatographic techniques yielded fourteen compounds, including five new compounds. The new compounds were determined to comprise an isoflavonoid structure with hydroxyethyl moiety and defined as hydroxyethylisoflavones A-E (10-14) on the basis of 1D and 2D NMR and MS data. These compounds efficiently inhibited α-glucosidase activity.

scholarly journals Isolation and purification of plant secondary metabolites using column-chromatographic technique

2016 ◽  
Vol 11 (4) ◽  
pp. 844 ◽  
Author(s):  
Vivek K. Bajpai ◽  
Rajib Majumder ◽  
Jae Gyu Park
Keyword(s):  
Secondary Metabolites ◽  
High Performance ◽  
Plant Secondary Metabolites ◽  
Biologically Active ◽  
Chromatographic Technique ◽  
Isolation And Purification ◽  
Chromatographic Techniques ◽  
Visual Demonstration ◽  
Layer Chromatography ◽  
Column Chromatographic

<p>Chromatographic techniques have significant role in natural products chemistry as well as contribute dramatically in the discovery of novel and innovative compounds of pharmaceutical and biomedical importance. This study focused on step-by-step visual demonstration of fractionation and isolation of biologically active plant secondary metabolites using column-chromatographic techniques. Isolation of bioactive compounds using column-chromatographic involves: a) Preparation of sample; b) Packing of column; c) Pouring of sample into the column; d) Elution of fractions; and e) Analysis of each fractions using thin layer chromatography. However, depending on nature of research, compounds can be further purified using high performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR) spectral analyses.</p><p><strong>Video Clips</strong></p><p><a href="https://www.youtube.com/v/pr8mrBoI8xA">Part 1:</a> 3 min 45 sec</p><p><a href="https://www.youtube.com/v/rYrfClKn-og">Part 2:</a> 6 min 21 sec</p><p><a href="https://www.youtube.com/v/kffHXxuPwbo">Part 3</a>: 4 min 45 sec</p>

scholarly journals Studies on the Chemistry of Lichens, IVX. Chemical Investigation of the Lichen Species Anaptychia fusca, Peltigera canina, and Omphalodiscus spodochrous

1975 ◽  
Vol 30 (7-8) ◽  
pp. 445-450 ◽  
Author(s):  
Yngve Solberg
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Linoleic Acid ◽  
Magnetic Resonance ◽  
Lichen Species ◽  
Chromatographic Techniques ◽  
Nuclear Magnetic Resonance Spectra ◽  
Chemical Content ◽  
Peltigera Canina ◽  
Column Chromatographic ◽  
Gyrophoric Acid

Abstract The three lichen species Anaptychia fusca, Peltigera canina, and Omphalodiscus spodochrous have been studied with regard to their chemical content. Atranorin, gyrophoric acid, tenuiorin, allantoin, taurine, linoleic acid, a new sterol-C31H52O2 , and waxes have been determined. The identity of the compounds was established by consideration of infrared, mass and nuclear magnetic resonance spectra. Besides, thin layer and column chromatographic techniques were used in this work.

Performance assessment of the GammafloTM automated radioimmunoassay system by assaying for digoxin.

1979 ◽  
Vol 25 (7) ◽  
pp. 1254-1258 ◽  
Author(s):  
R Valdes ◽  
G Savory ◽  
D Bruns ◽  
B Renoe ◽  
J Savory ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Continuous Operation ◽  
Automated System ◽  
System Operation ◽  
Chromatographic Techniques ◽  
Automated Method ◽  
Performance System ◽  
Assay Precision ◽  
Digoxin Radioimmunoassay ◽  
Column Chromatographic

Abstract We report an evaluation of the GammafloTM automated continuous-flow radioimmunoassay instrument in which we used a digoxin assay to assess system performance. System operation was based on combined continuous-flow and column-chromatographic techniques. No drift or carryover was detectable in 180 within-assay consecutive determinations performed at a rate of 42 determinations per hour (5 h of continuous operation). Within-assay and between-assay precision were less than 6% (coefficient of variation). The automated method correlated well (r = 0.960 and 0.952, respectively) with two established manual digoxin radioimmunoassay procedures. The data suggest this automated system offers a valid alternative to manual radioimmunoassay procedures in terms of overall precision, simplicity of operation, and sample throughout capacity.

Improved column-chromatographic isolation of glycosylated hemoglobin S.

1985 ◽  
Vol 31 (4) ◽  
pp. 631-633
Author(s):  
C Cirotto ◽  
M Allegrucci ◽  
M G Cartechini ◽  
P Compagnucci ◽  
F Santeusanio ◽  
...  
Keyword(s):  
Glycosylated Hemoglobin ◽  
Beta Subunits ◽  
Hemoglobin S ◽  
Chromatographic Techniques ◽  
Colorimetric Test ◽  
Accurate Temperature ◽  
A Minor ◽  
Accurate Temperature Control ◽  
Chromatographic Isolation ◽  
Column Chromatographic

Abstract We investigated glycosylated hemoglobin S by means of chromatography on Bio-Rex 70. The selected elution conditions were similar to those described by Trivelli et al. (N Engl J Med 284: 353-357, 1971), except for modified ionic strength and accurate temperature control. This enabled us to isolate a minor hemoglobin fraction whose properties, as determined by chromatography, electrophoresis, and two-dimensional maps of its tryptic peptides, were typical of a hemoglobin S tetramer with blocked N-terminal residues of the beta subunits. The colorimetric test indicated this to be a glycosylated hemoglobin. This procedure is notably improved over previous chromatographic techniques for isolating glycosylated hemoglobin S. Moreover, it can be easily used in any laboratories where Trivelli's method is routinely in use.

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