Development of high throughput screening protocol and identification of novel sources of resistance against bakanae disease in rice (Oryza sativaL.)

2014 ◽  
Vol 74 (4) ◽  
pp. 414 ◽  
Author(s):  
R. Abdul Fiyaz ◽  
S. Gopala Krishnan ◽  
H. Rajashekara ◽  
Ashutosh K. Yadav ◽  
B. M. Bashyal ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Sources Of Resistance ◽  
Bakanae Disease ◽  
Screening Protocol

scholarly journals High-throughput computational-experimental screening protocol for the discovery of bimetallic catalysts

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Byung Chul Yeo ◽  
Hyunji Nam ◽  
Hyobin Nam ◽  
Min-Cheol Kim ◽  
Hong Woo Lee ◽  
...  
Keyword(s):  
High Throughput ◽  
Bimetallic Catalysts ◽  
High Throughput Screening ◽  
Direct Synthesis ◽  
Catalytic Performance ◽  
Platinum Group Metals ◽  
Pt Catalyst ◽  
Electronic Density ◽  
Bimetallic Alloy ◽  
Screening Protocol

AbstractTo accelerate the discovery of materials through computations and experiments, a well-established protocol closely bridging these methods is required. We introduce a high-throughput screening protocol for the discovery of bimetallic catalysts that replace palladium (Pd), where the similarities in the electronic density of states patterns were employed as a screening descriptor. Using first-principles calculations, we screened 4350 bimetallic alloy structures and proposed eight candidates expected to have catalytic performance comparable to that of Pd. Our experiments demonstrate that four bimetallic catalysts indeed exhibit catalytic properties comparable to those of Pd. Moreover, we discover a bimetallic (Ni-Pt) catalyst that has not yet been reported for H2O2 direct synthesis. In particular, Ni61Pt39 outperforms the prototypical Pd catalyst for the chemical reaction and exhibits a 9.5-fold enhancement in cost-normalized productivity. This protocol provides an opportunity for the catalyst discovery for the replacement or reduction in the use of the platinum-group metals.

scholarly journals A Comparative High-Throughput Screening Protocol to Identify Entry Inhibitors of Enveloped Viruses

2013 ◽  
Vol 19 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Juan Wang ◽  
Han Cheng ◽  
Kiira Ratia ◽  
Elizabeth Varhegyi ◽  
William G. Hendrickson ◽  
...  
Keyword(s):  
High Throughput ◽  
Viral Entry ◽  
High Throughput Screening ◽  
Marburg Virus ◽  
Lassa Virus ◽  
Viral Pathogens ◽  
Enveloped Viruses ◽  
Entry Inhibitors ◽  
Screening Protocol ◽  
Therapeutic Prevention

Emerging and reemerging human viral pathogens pose great public health concerns since therapeutics against these viruses are limited. Thus, there is an urgent need to develop novel drugs that can block infection of either a specific virus or a number of viruses. Viral entry is thought to be an ideal target for potential therapeutic prevention. One of the challenges of developing antivirals is that most of these viruses are highly pathogenic and therefore require high biosafety-level containment. In this study, we have adopted a comparative high-throughput screening protocol to identify entry inhibitors for three enveloped viruses (Marburg virus, influenza virus H5N1, and Lassa virus) using a human immunodeficiency virus–based pseudotyping platform. We demonstrate the utility of this approach by screening a small compound library and identifying putative entry inhibitors for these viruses. One major advantage of this protocol is to reduce the number of false positives in hit selection, and we believe that the protocol is useful for inhibitor screening for many enveloped viruses.

High-Throughput Screening Protocol for the Coupling Reactions of Aryl Halides Using a Colorimetric Chemosensor for Halide Ions

2016 ◽  
Vol 18 (8) ◽  
pp. 1720-1723 ◽  
Author(s):  
Min Sik Eom ◽  
Jieun Noh ◽  
Han-Sung Kim ◽  
Soyeon Yoo ◽  
Min Su Han ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Aryl Halides ◽  
Coupling Reactions ◽  
Halide Ions ◽  
Colorimetric Chemosensor ◽  
Screening Protocol

scholarly journals Development of a Simple High-Throughput Screening Protocol Based on Biosynthetic Activity of Mycobacterium tuberculosis Glutamine Synthetase for the Identification of Novel Inhibitors

2006 ◽  
Vol 11 (8) ◽  
pp. 1035-1042 ◽  
Author(s):  
Upasana Singh ◽  
Dhiman Sarkar
Keyword(s):  
Enzyme Activity ◽  
Mycobacterium Tuberculosis ◽  
Glutamine Synthetase ◽  
High Throughput ◽  
High Throughput Screening ◽  
Quantitative Estimation ◽  
Signal To Noise ◽  
Chemical Library ◽  
Validation Data ◽  
Screening Protocol

A high-throughput screening protocol has been developed for Mycobacterium tuberculosis glutamine synthetase by quantitative estimation of inorganic phosphate. The Km values determined at pH 6.8 are 22 mM for L-glutamic acid, 0.75 mM for NH4Cl, 3.25 mM for MgCl2, and 2.5 mM for adenosine triphosphate. The Km value for glutamine is affected significantly by the increase in pH of assay buffer. At the saturating level of the substrate, the enzyme activity at pH 6.8 and 25° C is found to be linear up to 3 h. The reduction of enzyme activity is negligible even in presence of 10% DMSO. The Z′ factor and signal-to-noise ratio are found to be 0.75 and 6.18, respectively, when the enzyme is used at 62.5 μg/ml concentration. The IC50 values obtained at pH 6.8 for both L-methionine S-sulfoximine and DL-phosphothriacin are 500 μM and 30 μM, respectively, which is lowest compared to the values obtained at other pH levels. The Beckman Coulter high-throughput screening platform was found to take 5 h 9 min to complete the screening of 60 plates. For each assay plate, a replica plate is used to normalize the data. Screening of 1164 natural product fractions/extracts and synthetic molecules from an in-house library was able to identify 12 samples as confirmed hits. Altogether, the validation data from screening of a small set of an in-house library coupled with Z′ and signal-to-noise values indicate that the protocol is robust for high-throughput screening of a diverse chemical library.

scholarly journals A Modified High-Throughput Screening Protocol to Isolate Bacteriophages from Environmental Samples

2021 ◽  
Author(s):  
Abdallah Abdelsattar ◽  
Rana Nofal ◽  
Salsabil Makky ◽  
Amera El-Sayed ◽  
Ayman El-Shibiny
Keyword(s):  
Double Layer ◽  
High Throughput ◽  
Environmental Samples ◽  
High Throughput Screening ◽  
Phage Therapy ◽  
Resistant Bacteria ◽  
Sewage Sample ◽  
Antibiotic Resistant ◽  
Screening Protocol ◽  
Phage Isolation

In the post antimicrobial era, increasing attention is paid towards using bacteriophage (phage in short) therapy to control antibiotic-resistant bacteria. The first step in phage therapy applications is isolating highly efficient lytic phages or phage cocktails from various sources. When a double-layer- agar with around 0.7% agar in top agar is employed, it results in a low number of phage isolation with a poor resolution, and in many cases, you miss the phage. To address this problem, a low concentration of agar in top agar is examined for better phage isolation. Here, our results proved the efficiency of isolating phage upon formulating a double-layer agar with 0.3% agar in top agar. A sewage sample was collected then phages were isolated, purified, and spotted on a top layer agar with 0.3% agar. The results showed the possibility of isolating a higher number of phages on 0.3% top agar than 0.7%. The finding advocates using 0.3% top agar for the double-layer agar, as it will provide fast, better, and easy phage screening and isolation.

scholarly journals Energy-based descriptors for photo-catalytically active metal–organic framework discovery

2020 ◽  
Vol 8 (8) ◽  
pp. 4473-4482 ◽  
Author(s):  
Maria Fumanal ◽  
Gloria Capano ◽  
Senja Barthel ◽  
Berend Smit ◽  
Ivano Tavernelli
Keyword(s):  
High Throughput ◽  
Light Absorption ◽  
High Throughput Screening ◽  
Metal Organic Framework ◽  
Metal Organic Frameworks ◽  
Cost Effective ◽  
Active Metal ◽  
Catalytically Active ◽  
Screening Protocol ◽  
Metal Organic

A high-throughput screening protocol based on cost-effective computations of light absorption and photo-redox capabilities is proposed to discover promising photo-catalytically active metal–organic frameworks.

scholarly journals High-Throughput Screening—Based Identification of Paramyxovirus Inhibitors

2008 ◽  
Vol 13 (7) ◽  
pp. 591-608 ◽  
Author(s):  
Jeong-Joong Yoon ◽  
Dhruv Chawla ◽  
Tanja Paal ◽  
Maina Ndungu ◽  
Yuhong Du ◽  
...  
Keyword(s):  
High Throughput ◽  
Viral Entry ◽  
High Throughput Screening ◽  
Measles Virus ◽  
Human Pathogens ◽  
Response Curves ◽  
Major Morbidity ◽  
Screening Protocol ◽  
Hit Identification ◽  
Chemistry Journal

Several members of the paramyxovirus family constitute major human pathogens that, collectively, are responsible for major morbidity and mortality worldwide. In an effort to develop novel therapeutics against measles virus (MV), a prominent member of the paramyxovirus family, the authors report a high-throughput screening protocol that uses a nonrecombinant primary MV strain as targets. Implementation of the assay has yielded 60 hit candidates from a 137,500-entry library. Counterscreening and generation of dose-response curves narrows this pool to 35 compounds with active concentrations ≤15.3 µM against the MV-Alaska strain and specificity indices ranging from 36 to >500. Library mining for structural analogs of several confirmed hits combined with retesting of identified candidates reveals a high accuracy of primary hit identification. Eleven of the confirmed hits interfere with viral entry, whereas the remaining 24 compounds target postentry steps of the viral life cycle. Activity testing against selected members of the paramyxovirus family reveals 3 patterns of activity: 1) exclusively MV-specific blockers, 2) inhibitors of MV and related viruses of the same genus, and 3) broader range inhibitors with activity against a different Paramyxovirinae genus. Representatives of the last class may open avenues for the development of broad-range paramyxovirus inhibitors through hit-to-lead chemistry. ( Journal of Biomolecular Screening 2008:591-608)

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