scholarly journals SIMULTANEOUS QUANTITATIVE 1H NMR ANALYSIS OF METHANOL, ETHANOL AND THEIR METABOLIC PRODUCTS IN HUMAN PLASMA: EARLY DIANOSIS AND MONITORING DURING TREATMENT OF ACUTE METHANOL POISONING IN VIETNAM

2021 ◽  
Vol 64 (2) ◽  
pp. 41-48
Author(s):  
Ta Thi Thao ◽  
Nguyen Van Thuc ◽  
Pham Quang Trung ◽  
Nguyen Thi Ngan ◽  
Vu Anh Phuong ◽  
...  
Keyword(s):  
Detection Limit ◽  
Human Plasma ◽  
1H Nmr ◽  
Plasma Sample ◽  
Pulse Sequence ◽  
Water Suppression ◽  
Methanol Poisoning ◽  
Water Signal ◽  
Metabolic Products ◽  
Instrument Detection Limit

The 1H-NMR method for simultaneous quantification of methanol, ethanol and their metabolic products (formic acid and acetic acid) in human plasma was established. The effect of protein in plasma sample was removed by 25% trichloroacetic acid (TCA) with the ratio of TCA and plasma sample at 1/5 (v/v). The small amount of D2O (estimated 1/10 v/v) was chosen due to the effect of water signal was eliminated by using the advance water suppression with NOESY pulse sequence. The different analytical parameters such as linearity, precision, accuracy, and specificity, instrument detection limit (IDL) and instrument quantification limit (IQL) were validated. The instrument detection limit of four compounds in blank plasma varied from 0.68 (for methanol) to 2.88 mg/L (for formic acids). The average recoveries of concentration in dynamic ranges were found to be 96 to105%. The good linearity between concentration calculated by integrated signal and measured obtained by calibration curves confirmed that concentration of analytes in plasma can be directly determined from the 1H-NMR signal. The proposed method has been successfully applied for direct determination of five compounds in 9 patient’s plasma samples with the satisfactory results compared to GC/FID method. The analytical procedure can be useful for diagnosis and evaluation of treatment of methanol poisoning in Vietnamese hospitals.

Two-dimensional 1H NMR spectroscopy of normal and pathological human plasma: complete water suppression and further assignment of resonances

Biochimie ◽  
1992 ◽  
Vol 74 (9-10) ◽  
pp. 913-918 ◽  
Author(s):  
M. Kriat ◽  
S. Confort-Gouny ◽  
J. Vion-Dury ◽  
P. Viout ◽  
P.J. Cozzone
Keyword(s):  
Nmr Spectroscopy ◽  
Human Plasma ◽  
1H Nmr ◽  
1H Nmr Spectroscopy ◽  
Water Suppression ◽  
Two Dimensional

Analysis of Changes in Signal Intensity of Choroid Plexus in MRI Using FLAIR-DW-EPI Pulse Sequence

2020 ◽  
Vol 3 (1) ◽  
pp. 9-15
Author(s):  
Jingyu Kim ◽  
◽  
Sang-Jin Im ◽  
Keyword(s):  
Choroid Plexus ◽  
Signal Intensity ◽  
Pulse Sequence ◽  
Signal Strength ◽  
Brain Parenchyma ◽  
Weighted Image ◽  
Diffusion Weighted ◽  
Water Signal ◽  
The Brain ◽  
Functional Problems

In this study, the signal intensity of choroid plexus, which is producing cerebrospinal fluid, is analyzed according to the FLAIR diffusion-weighted imaging technique. In the T2*-DW-EPI diffusion-weighted image, the FLAIR-DW-EPI technique, which suppressed the water signal, was additionally examined for subjects with high choroid plexus signals and compared and analyzed the signal intensity. As a result of the experiment, it was confirmed that the FLAIR-DW-EPI technique showed a signal strength equal to or lower than that of the brain parenchyma, and there was a difference in signal strength between the two techniques. As a result of this study, if the choroidal plexus signal is high in the T2 * -DW-EPI diffusionweighted image, additional examination of the FLAIR-DW-EPI technique is thought to be useful in distinguishing functional problems of the choroid plexus. In conclusion, if the choroidal plexus signal is high on the T2*-DW-EPI diffuse weighted image, it is thought that further examination of the FLAIR-DW-EPI technique will be useful in distinguishing functional problems of the choroidal plexus.

scholarly journals Carotenoid Profiling of a Red Seaweed Pyropia yezoensis: Insights into Biosynthetic Pathways in the Order Bangiales

Marine Drugs ◽  
2018 ◽  
Vol 16 (11) ◽  
pp. 426 ◽  
Author(s):  
Jiro Koizumi ◽  
Naoki Takatani ◽  
Noritoki Kobayashi ◽  
Koji Mikami ◽  
Kazuo Miyashita ◽  
...  
Keyword(s):  
1H Nmr ◽  
Metabolic Pathways ◽  
Pyropia Yezoensis ◽  
Biosynthetic Pathways ◽  
Red Seaweed ◽  
Red Seaweeds ◽  
Natural Pigments ◽  
Photosynthetic Organisms ◽  
Metabolic Products ◽  
Β Carotene

Carotenoids are natural pigments that contribute to light harvesting and photo-protection in photosynthetic organisms. In this study, we analyzed the carotenoid profiles, including mono-hydroxy and epoxy-carotenoids, in the economically valuable red seaweed Pyropia yezoensis, to clarify the detailed biosynthetic and metabolic pathways in the order Bangiales. P. yezoensis contained lutein, zeaxanthin, α-carotene, and β-carotene, as major carotenoids in both the thallus and conchocelis stages. Monohydroxy intermediate carotenoids for the synthesis of lutein with an ε-ring from α-carotene, α-cryptoxanthin (β,ε-caroten-3’-ol), and zeinoxanthin (β,ε-caroten-3-ol) were identified. In addition, β-cryptoxanthin, an intermediate in zeaxanthin synthesis from β-carotene, was also detected. We also identified lutein-5,6-epoxide and antheraxanthin, which are metabolic products of epoxy conversion from lutein and zeaxanthin, respectively, by LC-MS and 1H-NMR. This is the first report of monohydroxy-carotenoids with an ε-ring and 5,6-epoxy-carotenoids in Bangiales. These results provide new insights into the biosynthetic and metabolic pathways of carotenoids in red seaweeds.

scholarly journals Monitoring of Irinotecan in Human Plasma: Sensitive Fluorescent Nanogels by Molecular Imprinting

2019 ◽  
Author(s):  
Martina Tommasini ◽  
Elena Pellizzoni ◽  
Valentina Iacuzzi ◽  
Elena Marangon ◽  
Paola Posocco ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fluorescence Quenching ◽  
Human Plasma ◽  
Molecular Imprinting ◽  
Plasma Sample ◽  
Direct Detection ◽  
Fluorescent Nanoparticles ◽  
High Dilution ◽  
Molecularly Imprinted

A series of fluorescent molecularly imprinted nanogels to detect irinotecan (CPT11) were prepared and characterized. A set of amino acids and napthalimide polymerisable derivatives allowed to obtain polymers as soluble fluorescent nanoparticles by high dilution imprinted synthesis. The direct detection of irinotecan in human plasma was obtained by fluorescence quenching of the naphtalimide-based imprinted materials. The plasma sample treated with acetonitrile allowed the detection of irinotecan in the 10nM – 30μM range. The LOD was 9.4 nM, with within-run variability 10% and day to day variability 13%.<br>

scholarly journals Selective Colorimetric Sensors for Cyanide and Acetate Ion in Partially Aqueous Medium

2018 ◽  
Author(s):  
Divya Singhal ◽  
Neha Gupta ◽  
Ashok Kumar Singh
Keyword(s):  
Detection Limit ◽  
Aqueous Medium ◽  
1H Nmr ◽  
Binding Constant ◽  
High Selectivity ◽  
Good Sensitivity ◽  
Dft Studies ◽  
Nmr Titration ◽  
1H Nmr Titration ◽  
Nanomolar Detection

4-(thiazol-2-yldiazenyl)phenol (L1) and 2-((4-hydroxyphenyl) diazenyl)-5-nitrophenol (L2) based on azo phenol were synthesised and used as selective colorimetric sensor for CN- and AcO&minus; ion in DMSO/H2O-HEPES (v/v; 1:1, pH&ndash;7.3 &plusmn; 0.2) and showed good sensitivity with large red shifts and nanomolar detection limit for CN- and AcO- ion. The stoichiometry of L1 with CN&minus;/AcO&minus; ion was found to be 1:1 and L2 with CN&minus;/AcO&minus; ion was found to be 1:2. Binding constant for L1+ CN&minus;, L1 + AcO&minus;, L2 + CN&minus; and L2 + AcO&minus; were calculated by B-H plot as 1.6 &times; 103, 8.0 &times; 102, 8.4 &times; 103 and 1.7 &times; 102 respectively. L2 showed high selectivity towards CN&minus; ion with low detection limit of 81 nM and large binding constant. In addition, 1H NMR titration and DFT studies also supported the deprotonation mechanism of receptors in the presence of selective anions.

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