THE IMPACT OF NUTRIENT MEDIUM COMPOSITION ON PROPAGATION OF PHLOX SIBIRICA L. IN IN VITRO CULTURE

Background:: Knowledge of the permeation characteristics of new chemical entities across biological membranes is essential to drug research and development. Transport medium composition may affect the absorption of compounds during in vitro drug transport testing. To preserve the predictive values of screening tests, the possible influence of transport media on the solubility of model drugs, and on the activities of tight junctions and efflux transporter proteins (e.g. P-glycoprotein) must be known. Objective:: The aim of this study was to compare the impact of different transport media on the bi-directional transport of standard compounds, selected from the four classes of the Biopharmaceutical Classification System (BCS), across excised pig intestinal tissue. Methods:: The Sweetana-Grass diffusion apparatus was used for the transport studies. Krebs-Ringer bicarbonate (KRB) buffer and simulated intestinal fluids in the fed (FeSSIF) and fasted (FaSSIF) states were used as the three transport media, while the chosen compounds were abacavir (BCS class 1), dapsone (BCS class 2), lamivudine (BCS class 3) and furosemide (BCS class 4). Results:: Abacavir exhibited lower permeability in both the simulated intestinal fluids than in the KRB buffer. Dapsone showed similar permeability in all media. Lamivudine exhibited lower permeability in FaSSIF than in the other two media. Furosemide exhibited improved transport with pronounced efflux in FaSSIF. Conclusion:: Different permeation behaviors were observed for the selected drugs in the respective media, which may have resulted from their different physico-chemical properties, as well as from the effects that dissimilar transport media components had on excised pig intestinal tissue.

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Jacob’s ladder Polemonium caeruleum L. and black salsify Sсorzonera hispanica L. in vitro culture

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v22.951 ◽

2018 ◽

Vol 22 ◽

pp. 216-221

Author(s):

O. V. Bulko ◽

L. G. Lioshina

Keyword(s):

In Vitro Culture ◽

Root Culture ◽

Medium Composition ◽

Cultivation Medium ◽

Plant Cultivation ◽

Regenerated Plants ◽

Composition Modification ◽

Jacob's Ladder ◽

Optimal Medium

Aim. Micropropagation of Jacob’s ladder Polemonium caeruleum L. and black salsify Scorzonera hispanica L., obtaining root culture and regenerated plants. Methods. In vitro plant cultivation, medium composition modification for micropropagation, inoculation of explants with agrobacterial strains. Results. In vitro cultures of Jacob’s ladder and black salsify have been obtained, the optimal medium composition has been determined for the effective plants multiplication, rooting and growth, root cultures and regenerated plants of studied species have been obtained. Conclusions. Obtained technology of in vitro culture establishment of P. caeruleum and S. hispanica can be used for plants microclonal propagation so as root culture and regenerated plants acquiring due to the agrobacterial transformation – for further studies of secondary metabolism of these plants. Keywords: P. caeruleum L., S. hispanica L., micropropagation, phytohormones, root culture.

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Introduction to in vitro culture and callus initiation in Salvia hispanica L. (chia)

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv ◽

10.7124/visnyk.utgis.17.1.1198 ◽

2019 ◽

Vol 17 (1) ◽

pp. 33-37

Author(s):

A. Z. Revutskaya ◽

A. V. Holubenko ◽

N. V. Nuzhyna ◽

H. O. Rudik ◽

N. Yu. Taran

Keyword(s):

In Vitro Culture ◽

Growth Regulators ◽

Nutrient Medium ◽

Callus Growth ◽

Basic Medium ◽

High Concentration ◽

Salvia Hispanica ◽

Callus Initiation ◽

Salvia Hispanica L

Aim. Preparation of aseptic seedlings Salvia hispanica L., callus initiation in vitro and establishment of primary explants suitable for the callus production. Methods. Seeds are sprouted on our own modification of conventional methods. The non-hormonal Murashige-Skoog agarized nutrient medium was used as basic medium for the experiments. Parts of one-month seedlings (roots, hypocotyl, cotyledon leaves) were used as explants for the use of the colza. We added growth regulators (BAP, 2,4-D) in different concentration combinations into the nutrient medium for callus initiation. Statistical processing was performed in Microsoft Office Excel. Results. Aseptic S. hispanica seedlings have been obtained. The callus growth was initiated on all types of explants, the dependence of the callus intensity on the type of explants and the growth regulators content in the nutrient medium was established. Morphogenic callus and root-regenerants have been obtained. Conclusions. Hypocotyl was the most suitable primary explant for callus growth. Seedlings, leaves and roots showed low morphogenetic capacity. The nutrient medium with an elevated 2,4-D content was the most effective for initiation of callus genesis and proliferation of non-morphogenous callus. A high concentration of 2,4-D in the medium improves S. hispanica callus growth but suppresses its morphogenic ability.Keywords: Salvia hispanica (Chia), in vitro culture, callus.

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Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition

Journal of Horticultural Research ◽

10.2478/johr-2014-0006 ◽

2014 ◽

Vol 22 (1) ◽

pp. 49-61 ◽

Cited By ~ 8

Author(s):

Rossen S. Sokolov ◽

Bistra Y. Atanassova ◽

Elena T. Iakimova

Keyword(s):

Nutrient Medium ◽

Culture Conditions ◽

Medium Composition ◽

Naphthaleneacetic Acid ◽

Axillary Shoots ◽

Regeneration Rate ◽

Primary Explants ◽

High Regeneration ◽

Basal Salts

AbstractThe objective of this study was to assess the regeneration response of in vitro cultured Magnolia × soulangeana ‘Alexandrina’ and Magnolia liliiflora ‘Nigra’ to nutrient medium composition. In the primary culture (initiated from dormant axillary buds) combinations of Murashige and Skoog (MS) basal salts with 6-benzylaminopurine and α-naphthaleneacetic acid were tested. The primary explants of cv. ‘Alexandrina’ expressed higher regeneration rate than cv. ‘Nigra’. For both species, the regen eration was most strongly potentiated at addition of 0.25 mg dm−3 of the cytokinin alone. The auxin exerted undesir–able effects. Several basal salts media were applied in proliferation stage and their physiological effects were evaluated in reference to traditionally used MS. At culturing on Chée & Pool C2d Vitis Medium (VM) that is for the first time introduced to magnolia and on MS, M. liliiflora formed more but less elongated shoots than M. soulangeana. However, on VM, substantial increase (25-30%) of the number of axillary shoots and leaves, shoot length and fresh and dry weights over MS was established for both species. This suggested VM as promising composition of nutrients in multiplication stage. Microshoots obtained on MS, VM, Rugini Olive Medium and DKW Juglans Medium were successfully rooted in vitro and subsequently established ex vitro. The findings expand the information on magnolia response to culture conditions and contribute to elaboration of innovative elements of protocols for establishing tissue cultures with high regeneration capacity.

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Clonal micropropagation of bog cranberry (Vaccinium oxycoccos L.) in the Udmurt Republic

Agricultural science Euro-North-East ◽

10.30766/2072-9081.2021.22.1.57-66 ◽

2021 ◽

Vol 22 (1) ◽

pp. 57-66

Author(s):

E. N. Cheremnykh ◽

T. G. Lekontseva ◽

A. V. Khudyakova ◽

A. V. Fedorov

Keyword(s):

In Vitro Culture ◽

Nutrient Medium ◽

Root Formation ◽

Sphagnum Moss ◽

Multiplication Factor ◽

Clonal Micropropagation ◽

Planting Material ◽

Udmurt Republic

The paper presents the results of 2018-2019 research on improving the technology of growing planting material of bog cranberry (Vaccinium oxycoccos L.) of Krasa Severa, Severyanka, Virussaare varieties on the basis of in vitro. Studied was the effect of the concentrations of growth regulators in the composition of the nutrient medium according to Anderson's recipe on the reproduction and subsequent rooting of micro cuttings, as well as the duration of cultivation and adaptation of micro plants depending on partial pruning of shoots. It has been established that at the stage of introduction into in vitro culture, sterilization of explants with 33% hydrogen peroxide in an exposure of 5-8 minutes with washing in 5 portions of sterile distillate gives 60-80 % of viable shoots. The optimum phase of plant development for the successful introduction of in vitro culture is the swelling of buds. Cultivation of micro cuttings was carried out in a light room at a temperature of 25±2 °С, a photoperiod of 16 hours. The duration of each subculturing was 30-60 days. For the stage of actual micropropagation on Anderson's nutrient medium, an increase in the dose of cytokinin 6-benzylaminopurine (6-BAP) from 0.2 to 0.5 mg/l and an increase in the duration of cultivation from 30 to 60 days contributed to a significant increase in the multiplication factor on average for the tested cranberry varieties.According to the efficiency of micropropagation, the varieties Virussaare and Krasa Severa were distinguished – 9.3-12.0 pcs/stalk, respectively. At the rooting stage, the use of a root-forming reagent of indolyl-3-acetic acid (IUK) in doses of 0.2, 0.5 and 1.0 mg/l in the composition of Anderson's nutrient medium did not affect the quality of root formation and the length of shoots of Virussaare micro-plants. No significant varietal differences in the root-forming ability of microcuttings were found. The tendency of better rooting of micro cuttings was observed in the Virussaare variety (90.3 %) compared to the Severyanka (85.7 %) and Krasa Severa (79.3 %) varieties. Micro plants of the Krasa Severa cultivar were characterized by the longest shoots, the total number of roots was less, but their length was longer in comparison with other cultivars. For the adaptation stage, a substrate from a mixture of lowland peat and sphagnum moss was used (1:1). The efficiency of adaptation of micro plants of cranberry varieties when cutting the tip of the shoots was 100 %. Pruning of micro plants shoots contributed to the formation of more side shoots and better development of the aboveground part of the plants.

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Regeneration capacity of Cerasus fruticosa and Prunus domestica into the in vitro culture

Agrarian Bulletin of the ◽

10.32417/1997-4868-2021-209-06-43-52 ◽

2021 ◽

Vol 209 (06) ◽

pp. 43-52

Author(s):

Marina Markova ◽

Elena Somova

Keyword(s):

In Vitro Culture ◽

Nutrient Medium ◽

Optimal Time ◽

In Vitro Cultivation ◽

Initiation Time ◽

Multiplication Factor ◽

Optimal Conditions ◽

Prunus Domestica ◽

Nutrient Media

Abstract. The aim of these studies was to introduce into the in vitro culture the steppe cherry (Cerasus fruticosa) variety Shchedraya and the domestic plum (Prunus domestica) variety Sineokaya for subsequent micropropagation. Methods. Optimal conditions for obtaining viable explants, such as sterilizing agent and initiation time, have been investigated. The suitability of various nutrient media for in vitro cultivation of these cultures has also been tested. As a result of the experiments, it was revealed that the most effective sterilizing agents were 38 % perhydrol (control) and 6% chlorhexidine: the yield of viable cherry explants was 63.8 % and 61.5 %, plums – 69.8 % and 66.6 %, respectively. The optimal time for the initiation of cherry explants in vitro was January, where the yield of viable explants averaged 53.9 %, in June – 49.1 %, and for plums the initiation time did not matter – the yield of explants was 55.8 % in winter and 53.1 % in summer. In vitro cultivation of cherries and plums on the Quoirin – Lepoivre nutrient medium provided a significantly high multiplication factor, which averaged 4.1 for cherries (2.7 in control) and 6.0 for plums (3.9 in control). On the same medium, the maximum multiplication factor was obtained, which was 6.2 for cherries and 8.2 for plums. Thus, the scientific novelty of these studies is that the optimal conditions (sterilizing agent, time, nutrient medium) have been selected for the regeneration of cherry and plum explants in vitro with their subsequent micropropagation.

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123 In Vitro Embryo Production in Lyophilized In Vitro Culture Medium as a Method to Increase the Medium's Shelf-Life

Reproduction Fertility and Development ◽

10.1071/rdv30n1ab123 ◽

2018 ◽

Vol 30 (1) ◽

pp. 201

Author(s):

M. Rubessa ◽

F. Salerno ◽

D. Weisgerber ◽

B. Gasparrini ◽

B. Harley ◽

...

Keyword(s):

In Vitro Culture ◽

Shelf Life ◽

Statistical Difference ◽

Cleavage Rate ◽

Embryo Production ◽

Freeze Dried ◽

In Vitro Embryo Production ◽

Α Level ◽

The Impact

The worldwide production of livestock embryos requires stable medium with long shelf life. In this experiment, we evaluated the impact of the freeze-dried in vitro culture (IVC) medium (Mdry) on in vitro embryo production. We compared the standard IVC and Mdry media for cleavage rate and embryo production. Media solutions (10 mL) were aliquoted into 50-mL conical tubes and lyophilized to form a powder concentrate using a Genesis freeze-dryer (VirTis, Gardener, NY, USA). Lyophilization consisted of a constant cooling from 20°C to –10°C at a constant rate of 1°C/min with a 2-h hold at –10°C before sublimation at 0°C. Mdry medium were held at –80°C for 4 months. When the IVC medium was rehydrated, the pH were adjusted to 7.4. Abattoir-derived Holstein oocytes (n = 618, in 7 replicates) were in vitro matured and fertilized with sexed semen, according to standard procedures (Rubessa et al. 2011 Theriogenology 76, 1347-1355). Twenty hours after IVF, presumptive zygotes were cultured in SOF medium with 5% BS at 39°C with 5% CO2, 7% O2, and 88% N2. On Day 7, embryo yields were assessed. All recorded parameters were subjected to a Chi-Square Test 2 × 2. The parameters compared were percent cleavage, blastocysts, and embryos/cleaved. The α level was set at 0.05. All data were expressed as quadratic means and mean standard errors. The results (Table 1) showed not a statistical difference between control and Mdry. The Mdry had a higher percentage of cleaved zygotes (65.4% v. 53.4%) but not enough for a statistical difference. However, when we compared embryo production, there was no difference between treatments. The ratio between blastocysts and cleaved embryos was higher in the control group but not significant according to our selected α level. These results indicate that it is possible lyophilize IVC medium without interfering with the potential quality of the medium. Further studies will be needed to better understand the positive effect of the lyophilization on the cleavage rate. Table 1.Mean (SD in parentheses) percentage cleavage and blastocysts

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Influence of the Nutrient Medium and Photoperiod on Tuberization of Potato Micro-plants of Promising Potato Varieties in vitro Culture

Bulletin of Science and Practice ◽

10.33619/2414-2948/49/18 ◽

2019 ◽

Vol 5 (12) ◽

pp. 177-181

Author(s):

E. Vlasevskaya ◽

I. Mukhametshin

Keyword(s):

In Vitro Culture ◽

Nutrient Medium ◽

New Technologies ◽

Sugar Concentration ◽

Potato Seed ◽

Potato Varieties ◽

Federal Research ◽

Production Technologies ◽

Academy Of Sciences

The results of studies on the influence of the nutrient medium and photoperiod on tuberization of micro-plants of promising potato cultivars in an in vitro culture under are presented the conditions of a potato renewal laboratory at the Udmurt Research Institute of Agriculture of the Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences in 2018. Two experiments were carried out to identify patterns of the influence of potato cultivation conditions in vitro on the efficiency of its propagation and to develop an improved method for propagating potato micro-tubers in vitro. The experiments were carried out in accordance with the recommendation “New technologies for the production of healthy source material in elite potato seed production”, “Technologies for microclonal propagation of plants”. Object of research: micro-plants of potato varieties Alena, Charodei, Nayada. The studied variants of sugar concentration are 0%, 2% (control), 4%, 6%, 8%, 10%. The studied photoperiod options are 16 hours (control), 14 hours, 12 hours, 10 hours, 8 hours, 6 hours, 4 hours. Based on the results of studies in 2018, experimental data were obtained. It was revealed that the lack of sugar in the nutrient medium negatively affects the growth and development of potato micro-plants. An increase in sugar concentration to 6–10%, in comparison with the control (2%), significantly increases the tuber-forming ability of micro-plants by an average of 6.1–7.2% and increases the number of tubers from one micro-plant. The highest yield of micro-tubers from one micro-plant on average for varieties was obtained with a 14-hour photoperiod and amounted to 1.3 pcs. With a 12-hour photoperiod, micro-tubers begin to form 7–14 days earlier than in the other variants of the experiment.

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The impact of medium composition and photosynthetically active radiation level on the initial in vitro growth and production of flavonoids of Vernonia condensata Baker

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2019.101063 ◽

2019 ◽

Vol 18 ◽

pp. 101063 ◽

Cited By ~ 3

Author(s):

Fabíola Rebouças Rodrigues ◽

Dayse Alessandra Almeida Silva Bispo ◽

Hugo Neves Brandão ◽

Taliane Leila Soares ◽

Weliton Antonio Bastos de Almeida ◽

...

Keyword(s):

Photosynthetically Active Radiation ◽

Medium Composition ◽

In Vitro Growth ◽

Radiation Level ◽

Active Radiation ◽

The Impact

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Effect of medium composition on the in vitro culture of bovine pre-antral follicles: morphology and viability do not guarantee functionality

Zygote ◽

10.1017/s0967199412000044 ◽

2012 ◽

Vol 21 (2) ◽

pp. 125-128 ◽

Cited By ~ 23

Author(s):

Rafael Rossetto ◽

Márcia Viviane Alves Saraiva ◽

Regiane Rodrigues dos Santos ◽

Cleidson Manoel Gomes da Silva ◽

Luciana Rocha Faustino ◽

...

Keyword(s):

In Vitro Culture ◽

Culture Media ◽

Medium Composition ◽

Minimum Essential Medium ◽

Antral Follicles ◽

Suitable Medium

SummaryThis study investigated the effect of three different culture media (α minimum essential medium (α-MEM), McCoy or TCM199 during the in vitro culture (IVC) of bovine isolated pre-antral follicles. Pre-antral follicles greater than 150 μm in size were isolated and cultured for 0 (control), 8 or 16 days in one of the abovementioned culture media. Follicles were evaluated for survival, growth and antrum formation at days 8 and 16. The results showed that TCM199 was the most suitable medium to preserve follicular viability and ultrastructure, resulting in the highest rates of antrum formation. In conclusion, TCM199 promotes the in vitro development of isolated pre-antral follicles without hampering follicular functionality by sustaining in vitro growth and antrum formation.

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