Assessment of the potential of Artemisia annua L. as a source of biologically active substances in Belarus

Aim. The aim of the work was to assess the composition of biologically active compounds in plants of Artemisia annua L. Methods. The plants were grown on the experimental plot of the Central Botanical Garden of the National Academy of Sciences of Belarus. Essential oil from the above-ground mass was isolated by the method of water-steam distillation. To analyze the content of flavonoids and hydroxycinnamic acids, we used the method of quantitative extraction and spectrophotometric determination of the total content of hydroxycinnamic acids in the presence of flavonoids in plant extractive substances, and the method of Folin-Chocalteu was used to determine the content of phenolic compounds. Analysis of the essential oils was performed by GC and NMR. The analysis of the content of phenolic acids in extracts was performed by HPLC. Results. The genetic potential of A. annua was evaluated under conditions of the central agroclimatic zone of Belarus. The yield and composition of essential oils, the content of phenolic compounds and flavonoids, the composition of phenolic acids were studied. Conclusions. A. annua in the conditions of the central agroclimatic zone of Belarus contains essential oil, which is dominated by isoartemisia ketone, β-selinene, β-myrcene and camphor. In the above-ground mass phenolic acids including chlorogenic acid and isomers of caffeoylquinic acid were predominant in the composition of phenolic compounds. Keywords: annual wormwood, essential oil, phenolic compounds, phenolic acids.

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INFLUENCE OF PLANTING DATE AND HARVEST TIME ON ARTEMISIA ANNUA L.

HortScience ◽

10.21273/hortsci.25.9.1165d ◽

1990 ◽

Vol 25 (9) ◽

pp. 1165d-1165 ◽

Cited By ~ 1

Author(s):

Ernst Cebert ◽

Denys J. Charles ◽

James E. Simon

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Artemisia Annua ◽

Planting Date ◽

Harvest Time ◽

Average Increase ◽

Optimum Time ◽

Planting Dates ◽

Oil Accumulation ◽

Artemisia Annua L

Artemisia annua L. is an aromatic and medicinal plant of importance for its volatile essential oils, and the non-volatile artemisinin used in the treatment of malaria. To determine the optimum time of planting for growth and the accumulation of essential oils, seedlings of A. annua (Purdue accession 012) were transplanted into the field in Central Indiana in a RBD with 3 replications on April 25, May 24, June 24, and July 25, 1988. Plant samples were harvested every 2 weeks until first frost.The April and May transplanting dates produced the tallest plants (>180 cm) while the May transplants accumulated the greatest fresh and dry weights. The average increase in plant height was greatest for the June 24 planting date at 9.8 cm per week. Regardless of planting date, all plants began to flower by early August and growth rate began to decrease by late August. Accumulation of essential oil (as rel. % dry wt.) was similar for all planting dates. Essential oil increased until floral initiation, then decreased for 2 weeks after which there was a rapid increase in oil accumulation. Maximum oil accumulation from all planting dates was reached on Sept. 28 after which growth continued to increase.

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Composition of essential oils and headspace constituents of Artemisia annua L. and A. scoparia Waldst. et Kit.

Journal of the Serbian Chemical Society ◽

10.2298/jsc200727061i ◽

2020 ◽

pp. 61-61

Author(s):

Jovana Ickovski ◽

Katarina Stepic ◽

Gordana Stojanovic

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Artemisia Annua ◽

The Other ◽

Static Headspace ◽

Main Constituent ◽

Headspace Volatiles ◽

First Report ◽

Artemisia Annua L ◽

Aerial Parts

Headspace volatiles (HS) and hydrodistilled essential oils (EO) of fresh aerial parts of Artemisia annua L. and A. scoparia Waldst. et Kit., were analyzed by GC-MS/FID. Artemisia ketone was found to be the most abundant component among the EO volatiles (55.8 %), as well as among HS (52.1 %) of A. annua. Additionally, in both A. annua samples, EO and HS, ?-pinene (12.7 and 24.2 %, respectively) was found in high percentage. On the other hand, it has been determined that the dominant components of A. scoparia EO and HS were different; in the essential oil capillene (63.8 %) was found as the main constituent, while ?-pinene (26.1 %), (Z)-?-ocimene (23.8 %) and limonene (10.7 %) were the major components among the HS. This is the first report on the composition of HS volatiles of the A. annua and A. scoparia obtained by direct static headspace.

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Comparative phytochemical study on the phenolic compounds content of Artemisia annua L. from wild populations in Republic of Moldova

Planta Medica ◽

10.1055/s-0036-1596344 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

CP Stefanache ◽

OC Bujor ◽

R Necula ◽

V Ghendov ◽

A Trifan ◽

...

Keyword(s):

Phenolic Compounds ◽

Artemisia Annua ◽

Wild Populations ◽

Phytochemical Study ◽

Republic Of Moldova ◽

Artemisia Annua L

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Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Applied Microbiology and Biotechnology ◽

10.1007/s00253-021-11101-8 ◽

2021 ◽

Vol 105 (3) ◽

pp. 1215-1226

Author(s):

Wojciech Makowski ◽

Aleksandra Królicka ◽

Anna Nowicka ◽

Jana Zwyrtková ◽

Barbara Tokarz ◽

...

Keyword(s):

Phenolic Compounds ◽

Phenolic Acids ◽

Antibacterial Properties ◽

Biologically Active ◽

Plant Genome ◽

Resistant Bacteria ◽

Shoot Cultures ◽

Transformed Plants ◽

Dionaea Muscipula ◽

Bactericidal Properties

Abstract The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D. muscipula tissues is presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 and ATCC 15834) were suitable vector organisms in the transformation process. Transformation led to the formation of teratoma (transformed shoot) cultures with the bacterial rolB gene incorporated into the plant genome in a single copy. Using high-pressure liquid chromatography, we demonstrated that transgenic plants were characterized by an increased quantity of phenolic compounds, including 1,4-naphtoquinone derivative, plumbagin (up to 106.63 mg × g−1 DW), and phenolic acids (including salicylic, caffeic, and ellagic acid), in comparison to non-transformed plants. Moreover, Rhizobium-mediated transformation highly increased the bactericidal properties of teratoma-derived extracts. The antibacterial properties of transformed plants were increased up to 33% against Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli and up to 7% against Pseudomonas aeruginosa. For the first time, we prove the possibility of D. muscipula transformation. Moreover, we propose that transformation may be a valuable tool for enhancing secondary metabolite production in D. muscipula tissue and to increase bactericidal properties against human antibiotic-resistant bacteria. Key points • Rhizobium-mediated transformation created Dionaea muscipula teratomas. • Transformed plants had highly increased synthesis of phenolic compounds. • The MBC value was connected with plumbagin and phenolic acid concentrations.

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Toxic essential oils. Part II: Chemical, toxicological, pharmacological and microbiological profiles of Artemisia annua L. volatiles

Food and Chemical Toxicology ◽

10.1016/j.fct.2013.04.016 ◽

2013 ◽

Vol 58 ◽

pp. 37-49 ◽

Cited By ~ 45

Author(s):

Niko S. Radulović ◽

Pavle J. Randjelović ◽

Nikola M. Stojanović ◽

Polina D. Blagojević ◽

Zorica Z. Stojanović-Radić ◽

...

Keyword(s):

Essential Oils ◽

Artemisia Annua ◽

Artemisia Annua L

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Comparative investigation of sesquiterpene components of essential oils originating from intact plants and hairy root chamomile cultures

GSC Advanced Research and Reviews ◽

10.30574/gscarr.2021.6.2.0016 ◽

2021 ◽

Vol 6 (2) ◽

pp. 028-049

Author(s):

Éva Szőke ◽

Éva Lemberkovics

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Hairy Root ◽

Folk Medicine ◽

Root Culture ◽

Hairy Root Culture ◽

Comparative Investigation ◽

Biologically Active

The importance of chamomile (Chamomilla recutita) inflorescence is widely known in classical and folk medicine, with the largest group of its effective constituents forming the essential oil (chamazulene, a-bisabolol, α-farnesene, trans-β-farnesene, spathulenol, cis/trans-en-in-dicycloethers). Among cultivated species, the Hungarian BK-2 contains more chamazulene in its essential oil than the German Degumil type, which is mainly cultivated for its a-bisabolol. Both components have important antiinflammatory activities. Wild populations can be easily distinguished from cultivated ones by their high amount of bisaboloides, particularly the flower of Hungarian Szabadkígyós wild type, which contained on average 48 % of the biologically active (-)-a-bisabolol. The population of Szabadkígyós has good salt tolerance which is important owing to global warming, because the proportion of saline areas is increasing worldwide. To keep the genome of Szabadkígyós having high (-)-a-bisabolol content, Szőke and research team used biotechnological methods. Sterile plantlets, were infected by Agrobacterium rhizogenes strains #A-4, #15834, #R-1601. The hairy root clones possessing the best growing and biosynthetical potential were multiplied for phytochemical investigations. Pharmacologically important compounds of their essential oils were followed in great detail. The amount of in vitro cultured terpenoids and polyin compounds was compared with that of in vivo plants. GC-MS studies showed that sterile chamomile cultures generated the most important terpenoid and polyin compounds characteristics of the mother plant. Berkheyaradulene, geranyl-isovalerat and cedrol as new components were identified in these sterile cultures. The main component of hairy root cultures (D/400, D/1, D/100 and Sz/400) was tr-b-farnesene and in addition one new compound: a-selinene was identified. Hairy root culture originated from chamomile collected in Szabadkígyós was intensive increased the essential oil content and pharmacological active compounds: (-) -α-bisabolol and β-eudesmol was also synthetized in large quantity. Furthermore, in vitro organized cultures were made from this population to obtain propagation material containing numerous active substances.

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GC MS Analysis of the Volatile Constituents of Essential Oil and Aromatic Waters of Artemisia Annua L. at Different Developmental Stages

Natural Product Communications ◽

10.1177/1934578x0800301225 ◽

2008 ◽

Vol 3 (12) ◽

pp. 1934578X0800301 ◽

Cited By ~ 4

Author(s):

Anna Rita Bilia ◽

Guido Flamini ◽

Fabrizio Morgenni ◽

Benedetta Isacchi ◽

Franco FrancescoVincieri

Keyword(s):

Essential Oil ◽

Developmental Stages ◽

Artemisia Annua ◽

Volatile Oil ◽

Flowering Stage ◽

Development Stages ◽

Qualitative And Quantitative ◽

Artemisia Annua L ◽

Germacrene D ◽

Long Time

Artemisia annua L. (Asteraceae) still represents the only source of artemisinin, considered as one of the most important drugs for the treatment of malaria and which, more recently, has been shown to be effective against numerous types of tumors. The foliage and inflorescence of A. annua also yield an essential oil upon hydrodistillation. This oil has been evaluated at different development stages (pre-flowering and flowering) by GC/MS. The volatile oil from plants at full blooming showed numerous constituents, with germacrene D (21.2%), camphor (17.6%), β-farnesene (10.2%), β-caryophyllene (9%), and bicyclogermacrene (4.2%) among the main ones. Aromatic waters, after extraction with n-hexane, showed the presence, among others, of camphor (27.7%), 1,8-cineole (14%), artemisia ketone (10.1%), α-terpineol (6.1%), trans-pinocarveol (5.4%), and artemisia alcohol (2%). From plants at the pre-flowering stage, aromatic waters were obtained with camphor (30.7%), 1,8-cineole (12.8%), artemisia alcohol (11.4%), artemisia ketone (9.5%), alpha-terpineol (5.8%), and trans-pinocarveol (3.0%) as the main constituents. The qualitative and quantitative profiles of the two aromatic waters were similar. These results permitted the conclusion to be made that A. annua could be harvested a long time before the onset of flowering to obtain higher yields of artemisinin or could be allowed to attain maturity to obtain valuable yields of volatiles.

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