scholarly journals Effect of medium gelatinized component on the effectiveness of androgenesis in vitro Oryza sativa L.

2016 ◽  
Vol 14 (2) ◽  
pp. 178-180
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
D. V. Shpak ◽  
A. O. Dobrova ◽  
S. O. Ignatova
Keyword(s):  
Anther Culture ◽  
Culture Medium ◽  
Oryza Sativa L ◽  
Modified Starch ◽  
Culture In Vitro ◽  
Chemically Modified ◽  
Androgenesis In Vitro ◽  
Negative Effect ◽  
Callus Regeneration

Aims. To study the effect of chemically modified starch D–5aM in the culture medium on the efficiency of androgenesis in vitro in anther culture of rice. Methods. Obtaining of rice double haploid lines by anther culture in vitro. The statistical methods. Results The influence different variants of gellatyne source in culture medium on the processes of induction and regeneration in anther culture of rice were studied. The 119 green plants-regenerants were received. Conclusions. The negative effect on the formation of green regenerants using a gel-forming components of the chemically modified starch D–5aM was shown. Keywords: rice, anther culture in vitro, callus, regeneration, chemically modified starch.

scholarly journals Effect of trophic, osmotically active and solidifying components of nutrient medium on the direct embryognesis in spring barley anther culture in vitro

2018 ◽  
Vol 22 ◽  
pp. 210-215
Author(s):  
E. V. Belinskaya ◽  
P. G. Dulnyev
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Spring Barley ◽  
Gelling Agent ◽  
Modified Starch ◽  
Hordeum Vulgare L ◽  
Culture In Vitro ◽  
Chemically Modified ◽  
Determine Mechanism

Aim. Ambryoidogenesis, or somatic embryogenesis, is known to be the most efficient mode of plant regeneration in plant cell, tissue and organ culture. The investigation was aimed to elucidate effects of trophic and osmogenic components of inductive medium on the frequency of direct embryoidogenesis in spring barley anther culture in vitro and to determine mechanism of morphogenesis improvement coursed by chemically modified starch D-5aM used as a gelling agent instead of agar. Methods. Anthers of DH-line with a high androgenetic capacity were inoculated on inductive media containing N6 macro-, MS micronutrients, organic supplements, maltose or mannitol (0.3 M) and solidified with agar or chemically modified starch. Results. A positive effect of combination of high maltose content and chemically modified starch on the induction and regeneration processes in spring barley anther culture in vitro was confirmed. It was also shown that mannitol didn’t keep any growth or development processes going in barley anther and embryo culture, but at the same time this substance had no toxic effect. Conclusions. In order to achieve a high frequency of induction in spring barley anther culture, it is necessary to use medium containing maltose – a low weight component both with trophic and with osmotic activity. Keywords: Hordeum vulgare L., anther culture in vitro, mannitol, maltose, embryo formation, plant regeneration.

scholarly journals Efficiency of spring barley haploid production in anther culture in vitro: comparison of basic and improved technologies

2019 ◽  
Vol 25 ◽  
pp. 178-183
Author(s):  
O. V. Bilinska ◽  
P. G. Dulnyev
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Spring Barley ◽  
Gelling Agent ◽  
Improved Method ◽  
Cold Pretreatment ◽  
Culture In Vitro ◽  
Chemically Modified ◽  
Modified Starches

Aim. Evaluation of innovative methodological approaches elaborated on model genotypes for usability to increase frequencies of morphogenic structure induction and plant regeneration in anther culture in vitro in spring barley diverse material. Methods. Spikes isolated from F1 and F2 hybrids of four crosses were pretreated using an improved method (4ºC, 28 days), and anthers were inoculated onto nutrient media containing chemically modified starches D5-M and D5a-1 instead of agar. In control cut tillers were emerged in water and pretreated at 4ºC for 5 days. Anthers were cultivated on agar solidified medium. Results. Positive effects of the improved method of cold pretreatment and cultivation of anthers on media solidified with starches were confirmed. The advantage of new gelling agent D5a-1 was proved. Particularly, its usage resulted in a three-fold increase in the frequency of green plant regeneration. Conclusions. In order to increase spring barley androgenic haploid yield, combination of prolonged cold pretreatment with anther cultivation on media solidified with chemically modified starches instead of agar in an integrated technological process is reasonable. Keywords: Hordeum vulgare L., anther culture in vitro, cold pretreatment, starch, agar, embryo formation, plant regeneration.

scholarly journals Variability of rice haploids obtained in anther culture in vitro

2019 ◽  
pp. 11-14
Author(s):  
M. V. Ilyushko ◽  
M. V. Romashova
Keyword(s):  
Anther Culture ◽  
Plant Height ◽  
Oryza Sativa L ◽  
Doubled Haploids ◽  
Morphological Variability ◽  
Callus Line ◽  
Culture In Vitro ◽  
Panicle Number ◽  
Callus Lines

Morphological variability of haploid plants and doubled haploids of rice obtained on one callus line in anther culture in vitro was studied. The work was carried out on rice plants Oryza sativa L. subspecies japonica Kato varietу Cascade. Regenerant plants of one callus line obtained from one rice anther (four in total) were divided into two or three groups of 20-30 plants, depending on the sample size in order of their differentiation on callus and transplantation on the rooting medium. Two callus lines (15.1 and 18.1) formed half of the haploids, half of the doubled haploids, and two other callus lines (5.1 and 7.2) numerous haploids. On callus lines with numerous haploids (5.1 and 7.2), as the regenant number increases, the size of plants decreases (plant height, number of flowers on the main panicle, number of panicles). On the lines 15.1 and 18.1 between groups of haploids and between groups of doubled haploids statistically significant differences absent. In breeding purposes for the induced doubling of the number of chromosomes in haploid regenerants with antitubulin substances such as colchicine, it is advisable to use plants that form on callus among the first. Between haploids of four callus lines and doubled haploids of two callus lines, statistically significant differences (at p=0.001) were revealed using the Hotelling's T2-criterion, calculated for the whole complex of biometric features. Haploids of different lines differed in three or four of them, doubled haploids on three of the five signs (length of panicle, productive bushiness and plant height). Varieties of interest to breeders may be improved by anther culture in vitro.

scholarly journals Stabilization of soft wheat selection material which is the result of crossing with wild relatives

2021 ◽  
Vol 28 ◽  
pp. 83-87
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
T. P. Nargan ◽  
M. S. Chekalova
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Callus Formation ◽  
Wild Relatives ◽  
Soft Wheat ◽  
Ability To Regenerate ◽  
Androgenesis In Vitro ◽  
Callus Regeneration ◽  
Soft Winter Wheat

Aim. Testing the haploproduction ability of 30 hybrids of winter soft wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of callus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The microspores of 17 of 30 hybrids formed callus by in vitro anther culture were shown. The intensity of one process was different: more than half of the genotypes (18 pcs.) were characterized by a low percentage of callus (from 0.10 to 1.0%), 6 genotypes - medium (from 1.0 to 3.0%), and three - high (4.36%; 15.11% and 15.81%, respectively). Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed Samples P26 and P27 showed the highest level of callus formation. The 10 green regenerating plants were obtained. Keywords: hybrids, soft winter wheat, anther culture in vitro, callus, regeneration.

scholarly journals The efficiency of androgenesis in vitro in anther culture of soft wheat winter varieties and their different generations hybrids

1970 ◽  
pp. 194-197
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
M. S. Boiko ◽  
H. O. Dobrova ◽  
S. V. Ahafonova
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Bread Wheat ◽  
Culture In Vitro ◽  
Winter Bread Wheat ◽  
Effective Technology ◽  
Androgenesis In Vitro ◽  
Soft Winter Wheat ◽  
Wheat Hybrids

Aim. To estimate the level of in vitro haploproduction androgenic ability of different generations populations (F1, F2) soft winter wheat hybrids of the same combination to maximize the use of biotechnology cereals. Methods. Obtaining of bread wheat double haploid lines by anther culture in vitro. The statistical methods. Results. The influence different generation of wheat hybrids on the processes of induction and regeneration in anther culture of winter bread wheat were studied. The 21 green plants-regenerants were received. Conclusions. The using of anthers of the second generation simple hybrids as donor material for the effective technology creation of doubled haploid of bread winter wheat was shown.Keywords: wheat, anther culture in vitro, callus, regeneration, generation simple hybrids.

scholarly journals Grain barley starch as a gelling agent of nutrient media for cultivation of anthers and isolated embryos of H. vulgare L.

2020 ◽  
Vol 26 ◽  
pp. 158-163
Author(s):  
O. V. Bilynska
Keyword(s):  
Embryo Culture ◽  
Spring Barley ◽  
Gelling Agent ◽  
Modified Starch ◽  
Normal Type ◽  
Nutrient Media ◽  
Culture In Vitro ◽  
Chemically Modified ◽  
Pea Starch

Aim. Determination the possibility of agar replacement with normal and waxy type starches produced from barley seeds in the nutrient media for spring barley haploid production. Methods. Anthers of spring barley line DH00-126 were inoculated on inductive media containing N6 macro-, MS micronutrients, organic supplements, maltose (9.0 %) and differed in solidifying agents (agar, starch from barley seeds or chemically modified starch D5a-1). Trophic capacities of barley starch were determined both in anther and embryo culture of barley. Results. It was determined that barley starch of waxy type was not suitable for solidifying of nutrient medium. Use of normal type barley starch (6.5 %) resulted in sufficient decrease in the frequency of green plant regeneration. At the same time, this modification had no effect on the number of morphogenic anthers. Barley starch was inferior to pea starch in the ability to support seedling growth in embryo culture in vitro. Conclusions. Barley starch of normal type has worse trophic capacities in comparison to pea starch in embryo culture. It was appeared barley starch to be a less suitable gelling agent of medium for spring barley haploid production in anther culture in vitro than agar and chemically modified starch D5a-1. Keywords: Hordeum vulgare L., anther culture in vitro, embryo culture in vitro, agar, barley starch.

scholarly journals The testing of haploproduction ability of soft winter wheat different hybrids in anther culture in vitro

2020 ◽  
Vol 26 ◽  
pp. 207-211
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
M. S. Chekalova ◽  
E. A. Golub
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Hybrid Population ◽  
Culture In Vitro ◽  
Green Plants ◽  
Ability To Regenerate ◽  
Androgenesis In Vitro ◽  
Soft Winter Wheat ◽  
Range Of Variation

Aim. Study the the efficiency of androgenesis in vitro in anther culture of different genotyps winter wheat. Methods. Obtaining of soft winter wheat double haploid lines by anther culture in vitro. The statistical methods. Results The ability to androgenesis in an in vitro anthers culture of 30 soft winter wheat genotypes was tested. The differences in the frequency of callus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The range of variation haploproduction activiti (from % of implanted anthers) was broad. The sign of "the formation of callus" was in limited from 0 to 13.2 % and on the sign of "regeneration of green plants" – from 0 to 1.8 %. The 126 green plants-regenerants were received. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. It is advisable to use the anthers from the F2 hybrid population plant as a donor material to create an effective technology for producing double haploids of soft winter wheat was shown. Keywords: wheat hybrid, anther culture in vitro, callus, regeneration.

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