scholarly journals Requirements for breaking dormancy and germination characteristics of Toxicodendron vernicifluum seeds

2021 ◽  
Vol 47 (1) ◽  
pp. 99-104
Author(s):  
Shunsuke IMAI ◽  
Koji INAKI ◽  
Jun OBARA ◽  
Shigeharu KOGUSHI ◽  
Toshihiko KINUGASA
Keyword(s):  
Breaking Dormancy ◽  
Germination Characteristics ◽  
Toxicodendron Vernicifluum

Drought stress and osmo-priming effects on physiological and biochemical characteristics of Ocimum basilicum

2016 ◽  
Vol 6 (1) ◽  
pp. 905-913
Author(s):  
Bahram Majd Nassiry ◽  
Neda Mohammadi
Keyword(s):  
Drought Stress ◽  
Total Protein ◽  
Catalase Activity ◽  
Seedling Emergence ◽  
Seed Priming ◽  
Ocimum Basilicum ◽  
Field Development ◽  
Physiological And Biochemical Characteristics ◽  
Germination Characteristics ◽  
And Control

    One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development. Seed priming technique has been known as a challenge to improve germination and seedling emergence under different environmental stresses. The objectives of this research were to evaluate the effects of osmo-priming on germination characteristics and changes of proline, protein and catalase activity of Ocimum basilicum seeds. Results showed that drought stress reduced the germination characteristics and drought stress in -8 bar was the critical stress.  Priming treatments were include KNO3, PEG and NaCl by 0, -4 and -8 bar concentrations. The seeds were primed with those materials for 8 and 16 hours. The highest germination characteristics were obtained from nitrate potassium in -8 bar for 16 hours priming. Therefore the best seed treatment under drought stress during germination was obtained from the osmo-primed with -8 bar nitrate potassium for 16 hours. The drought stress increased proline and catalase activity but reduced total protein. Priming treatment increases proline, total protein and catalase activity under drought and control conditions. It is concluded that priming results in improvement in germination components of Ocimum basilicum in drought stress conditions and increases the resistance to drought stress with improvement of proline, protein and catalase activity in germination phase.

EFFECT OF Cd2+ STRESS ON SEED GERMINATION CHARACTERISTICS OF RYEGRASS, INDIAN MUSTARD AND GRAIN AMARANTH

2019 ◽  
Vol 18 (9) ◽  
pp. 1875-1884
Author(s):  
Zhang Jun ◽  
Wang Wenke ◽  
Geng Yani ◽  
Wang Zhoufeng ◽  
Cao Shumiao
Keyword(s):  
Seed Germination ◽  
Indian Mustard ◽  
Grain Amaranth ◽  
Germination Characteristics

scholarly journals Comprehensive analysis of the isozyme composition of laccase derived from Japanese lacquer tree, Toxicodendron vernicifluum

2021 ◽  
Vol 67 (1) ◽  
Author(s):  
Mariko Takano ◽  
Masaya Nakamura ◽  
Masanobu Tabata
Keyword(s):  
Isoelectric Focusing ◽  
Laccase Activity ◽  
Maximum Activity ◽  
Acetone Powder ◽  
Blue Color ◽  
Buffer Solution ◽  
Activity Staining ◽  
Water Extracts ◽  
Ph Range ◽  
Toxicodendron Vernicifluum

AbstractWe performed an analysis using isoelectric focusing to comprehensively clarify the isozyme composition of laccase derived from Japanese lacquer tree, Toxicodendron vernicifluum. When water extracts of acetone powder obtained from lacquer were subjected to isoelectric focusing, five bands within pI 7.35–9.30 and nine bands within pI 3.50–5.25 were detected using Coomassie staining. Similarly, laccase activity staining using guaiacol showed five bands within pI 7.35–9.30 and three bands within pI 3.50–4.25. However, laccase activity staining using gallic acid showed remarkable staining within pI 3.50–5.85, whereas staining was very weak within pI 7.35–9.30. When the water extracts of acetone powder were fractionated into the fractions containing bands within pI 7.35–9.30 and pI 3.50–5.85 by SP-Sepharose column chromatography, the former had a blue color and the latter a yellow color. The laccase activity was measured for each of the fractions in buffer solution in the pH range of 2.5–8.0. When syringaldazine, guaiacol, and 2,6-dimethoxyphenol were used as substrates, the yellow fraction showed considerably higher activity than the blue fraction for pH 5.5–7.5. When 3-methylcatechol and 4-methylcatechol were used as substrates, the yellow fraction showed higher activity for pH 4.5–6.5, and the blue fraction showed higher activity for pH 7.0–8.0. When 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) was used as the substrate, both fractions showed maximum activity at optimum pH of 3.0–4.0. Conventionally, in research on blue laccase derived from lacquer, the non-blue fraction corresponding to the yellow fraction lower than pI 6 has been removed during the purification process and thus has not been analyzed. Our results indicated that yellow laccase was present in the non-blue components of lacquer and that it may play a role in urushiol polymerization with previously reported blue laccase.

Germination Characteristics of Raphanus raphanistrum. I. Laboratory Studies

1975 ◽  
Vol 102 (5) ◽  
pp. 243 ◽  
Author(s):  
Michael R. Mekenian ◽  
Roger W. Willemsen
Keyword(s):  
Laboratory Studies ◽  
Raphanus Raphanistrum ◽  
Germination Characteristics

scholarly journals Potential Propagation by Seed and Cuttings of the Azorean NativeCalluna vulgaris(L.) Hull

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Maria João Pereira ◽  
Helena Fagundo ◽  
Tiago Menezes ◽  
João Couto
Keyword(s):  
Room Temperature ◽  
Seed Storage ◽  
Calluna Vulgaris ◽  
Landscape Conservation ◽  
Wild Populations ◽  
Surface Sterilization ◽  
Cultural Conditions ◽  
Germination Characteristics ◽  
Seed Age ◽  
Natural Stands

This work investigates the potential propagation by seed and cuttings of the Azorean nativeCalluna vulgaris(L.) Hull. for landscape conservation. With that purpose we have performed several germination and cuttings trials, using plant material from wild populations of this species. In the germination trials, we tested the effects of photoperiod length (8 and 16 h), temperature (10, 15, 20, and 20–10°C), seed age (6, 108, and 270 days), temperature of seed storage (4°C and room temperature), and seed surface sterilization on the germination characteristics. In the cuttings trials, we tested the effects of stem cutting type, cultural conditions, cuttings’ harvest month, and rooting substrates on the rooting percentages. The best percentages of germination, 93 and 90%, were obtained with fresh seeds and surface sterilized and sown under an 8 h photoperiod and with temperatures of 10°C or 15°C, respectively; germination after seed storage during 270 days is significantly superior (71%) when seeds are stored at 4°C. The best percentages of rooting were achieved for straight (96%) or heel cuttings (90%) harvested in March, planted on soil from natural stands ofC. vulgarisandErica azoricaHochst., outdoors in half shade, and partially covered with transparent polyethylene film.

Comparison of pre-treatments for inducing germination in highly dormant wheat genotypes

2003 ◽  
Vol 83 (4) ◽  
pp. 729-735 ◽  
Author(s):  
M. A. Matus-Cádiz ◽  
P. Hucl
Keyword(s):  
Seedling Growth ◽  
Biomass Yield ◽  
Triticum Aestivum L ◽  
Potassium Nitrate ◽  
Dormancy Breaking ◽  
Yield Data ◽  
Breeding Populations ◽  
Breaking Dormancy ◽  
Seedling Length ◽  
Pre Treatment

An effective dormancy-breaking method may be of interest to wheat (Triticum aestivum L.) breeders selecting for increased seed dormancy prior to advancing their populations in greenhouse grow-outs. The objective of this study was to identify an effective pre-treatment for breaking dormancy in wheat that did not result in seedling etiolation. In 2000, eight dormant (W98616, line 211, EMDR-4, EMDR-9, EMDR-14, RL4137, Columbus, and AC Domain) and one nondormant line (Roblin) were grown at two locations in Saskatchewan. Seeds were: (i) stored for zero to 21 wks at 24°C before incubating at 20°C for 7 d; (ii) incubated at 5, 10, 15, 20, and 25°C for 14 d; and (iii) treated with gibberellic acid (GA3) (0.0006 and 0.0014 M), potassium nitrate (KNO3) (0.01 and 0.02 M), chilling, heating, chilling with 0.01 M KNO3, and heating with 0.01 M KNO3 before incubating at 10°C for 14 d. Seedling growth was observed in a duplicated growth chamber experiment. Seedling length, first inter-node length, and biomass yield data were collected from plants grown from seeds treated with four effective pretreatments. Data were subjected to an ANOVA. Six to 18 weeks of storage at 24°C were required to break the dormancy (≥ 95% germination) in dormant genotypes. Incubation at 10°C was the most effective temperature for promoting germination in dormant seeds after 10d of testing. Four pre-treatments including 0.0006 M GA3, 0.0014 M GA3, chilling with 0.01 M KNO3, and heating with 0.01 M KNO3 led to ≥ 95% germination within 10 d of testing. Only GA3 treatments were associated with etiolated seedling growth. Heating with 0.01 M KNO3 or chilling with 0.01 M KNO3, applied before incubating at 10°C in darkness, may be of interest to breeders selecting for increased dormancy before advancing breeding populations in greenhouse grow-outs. Key words: Triticum, dormancy, nitrate, chilling, heating, etiolated seedling

Effects of light, temperature and population variability on the germination of seven Spanish pines

2002 ◽  
Vol 12 (4) ◽  
pp. 261-271 ◽  
Author(s):  
A. Escudero ◽  
F. Pérez-García ◽  
A. L. Luzuriaga
Keyword(s):  
Habitat Preferences ◽  
Population Variability ◽  
Nested Models ◽  
Germination Response ◽  
Germination Characteristics ◽  
Different Populations ◽  
Effects Of Temperature ◽  
Germination Traits ◽  
The Relationship ◽  
Pine Species

AbstractMost Pinus species are obligate seeders. Thus, knowledge of germination characteristics can help in the understanding, prediction and manipulation of the regeneration and dynamics of pine forests. Seven pine species with contrasting habitat preferences and different genetic pairwise distances are present in the Iberian Peninsula and the Canary Islands: P. halepensis, P. nigra, P. pinaster, P. pinea, P. sylvestris, P. uncinata and P. canariensis. These seven pine species comprise an exceptional experimental set to test some questions related to germination traits, such as: (1) What are the effects of light and temperature on germination, taking into account interpopulation variability? (2) Is there any association of germination traits with habitat (montane versus lowland) preferences? and (3) What is the relationship between germination traits and the genetic distance between pine species? P. nigra, P. sylvestris and P. uncinata seeds showed faster germination rates. Seeds of P. nigra and P. sylvestris reached high total germination percentages in every temperature and light treatment, suggesting an opportunistic germination strategy. Unlike montane pines, lowland pines did show significant effects of temperature on germination response: final germination was higher between 15°C and 20°C than at warmer and alternating temperatures. Relatively low temperatures associated with the winter rainy season would favour germination of most of these species. Nested models showed that population variability was the main source of variation in germination response. Thus, there is no phylogenetic control of the germination response and, surprisingly, germination traits were not related to habitat preferences. As a consequence, we believe that studies of the germination characteristics of a pine species should consider different populations.

Nitrogen fertilization stimulates germination of dormant pin cherry seed

1979 ◽  
Vol 9 (4) ◽  
pp. 514-516 ◽  
Author(s):  
L. R. Auchmoody
Keyword(s):  
Ammonium Sulfate ◽  
Nitrogen Fertilization ◽  
Forest Floor ◽  
Calcium Nitrate ◽  
Nitrogen Fertilizers ◽  
Triple Superphosphate ◽  
Breaking Dormancy ◽  
Muriate Of Potash

Nitrogen fertilizers triggered germination of dormant Prunuspensylvanica L. seed naturally buried in the forest floor of 60-year-old Allegheny hardwood stands. Neither triple superphosphate nor muriate of potash applied with urea increased germination over that which occurred with urea alone. Rates as low as 56 kg/ha N from urea and calcium nitrate and 112 kg/ha N from ammonium sulfate stimulated germination. Nitrate was apparently responsible for breaking dormancy.

Variation of fruiting and germination characteristics of Rhododendron aganniphum var. schiaopeplum with environmental factors

2021 ◽  
Vol 41 (1) ◽  
Author(s):  
杨紫玲，喻舞阳，郑维列，罗建，王国严，卢杰，李江荣 YANG Ziling
Keyword(s):  
Environmental Factors ◽  
Germination Characteristics
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