Potential Propagation by Seed and Cuttings of the Azorean NativeCalluna vulgaris(L.) Hull

This work investigates the potential propagation by seed and cuttings of the Azorean nativeCalluna vulgaris(L.) Hull. for landscape conservation. With that purpose we have performed several germination and cuttings trials, using plant material from wild populations of this species. In the germination trials, we tested the effects of photoperiod length (8 and 16 h), temperature (10, 15, 20, and 20–10°C), seed age (6, 108, and 270 days), temperature of seed storage (4°C and room temperature), and seed surface sterilization on the germination characteristics. In the cuttings trials, we tested the effects of stem cutting type, cultural conditions, cuttings’ harvest month, and rooting substrates on the rooting percentages. The best percentages of germination, 93 and 90%, were obtained with fresh seeds and surface sterilized and sown under an 8 h photoperiod and with temperatures of 10°C or 15°C, respectively; germination after seed storage during 270 days is significantly superior (71%) when seeds are stored at 4°C. The best percentages of rooting were achieved for straight (96%) or heel cuttings (90%) harvested in March, planted on soil from natural stands ofC. vulgarisandErica azoricaHochst., outdoors in half shade, and partially covered with transparent polyethylene film.

Download Full-text

Optimization of cultural conditions affecting improved bioactive metabolite production by endophytic fungus Trichoderma harzianum

Bionatura ◽

10.21931/rb/2021.06.04.8 ◽

2021 ◽

Vol 6 (4) ◽

pp. 2187-2192

Author(s):

Rashid Rahim Hateet ◽

Zainab Alag Hassan ◽

Abdulameer Abdullah Al-Mussawi ◽

Shaima Rabeea Banoon

Keyword(s):

Antibacterial Activity ◽

Trichoderma Harzianum ◽

Endophytic Fungus ◽

Nitrogen Sources ◽

Bioactive Metabolites ◽

Bioactive Metabolite ◽

Metabolite Production ◽

Surface Sterilization ◽

Cultural Conditions

The present study aimed to optimize cultural conditions for optimum bioactive metabolite production by endophytic fungus Trichoderma harzianum, isolated by surface sterilization method from the leaf of the eucalyptus plant. The fungus was identified based on morphological characterization. Fungal metabolites were carried out by ethyl acetate solvent. The antibacterial activity was tested against Escherichia coli (ATCC 25922) and Staphylococcus aureus (NCTC 6571). Various carbon, nitrogen sources, pH, temperature, incubation period, and NaCl on the antibacterial metabolite production were studied. Bioactive metabolite production of T. harzianum exhibits a broad spectrum of in vitro antibacterial activity against two strains of bacteria. For the optimum production of bioactive metabolites, Dextrose and Glucose were found to be the best sources of carbon and the best sources of Nitrogen Yeast extract (YE) and (NH4)2SO. The maximum production of bioactive metabolites occurs at pH 7 and 25°C.; the NaCl showed a positive influence on bioactive metabolites.

Download Full-text

Overcoming root dormancy and identifying the storage behaviour of Lilium polyphyllum seeds

Botany ◽

10.1139/cjb-2018-0161 ◽

2019 ◽

Vol 97 (2) ◽

pp. 161-166 ◽

Cited By ~ 1

Author(s):

Anurag Dhyani ◽

Carol C. Baskin ◽

Bhagwati Prasad Nautiyal ◽

Mohan Chandra Nautiyal

Keyword(s):

Room Temperature ◽

Seed Storage ◽

Seedling Production ◽

Spring Temperature ◽

Indole Butyric Acid ◽

Nitrogenous Compounds ◽

Morphophysiological Dormancy ◽

Mean Germination Time ◽

The Mean ◽

Endangered Medicinal Herb

Lilium polyphyllum D.Don ex Royle is a critically endangered medicinal herb of Himalaya. Seeds have epicotyl morphophysiological dormancy, and in the field a minimum of 34 weeks is required for root emergence. To date, treatments to decrease the time for root protrusion and seed storage behaviour have not been determined. We evaluated the effects of plant growth regulators and nitrogenous compounds as well as seed scarification on root emergence, and determined the storage behaviour of seeds. Root emergence at 20 °C was significantly increased by 100 ppm of indole butyric acid (IBA), KNO3, and thiourea, and by the application of NaOCl solution for 30 min. The mean germination time was significantly decreased by 50 ppm of gibberellic acid (GA3; 28 days) compared with 100 ppm GA3 (36 days). After 12 months of dry storage, the viability of seeds (TTC method) at room temperature (10–35 °C), 0–4 °C, and −20 ± 1 °C was 95%, 100%, and 100%, respectively; thus, the seeds have orthodox storage behaviour. Our recommendation for seedling production is to store the seeds at room temperature until they can be sown under warm wet conditions. Roots emerge after about 8 weeks, and then seeds should be given a 2-week cold moist treatment to break shoot dormancy. After 2 weeks of cold, transfer the seeds to warm (20 °C) spring temperature conditions to promote growth.

Download Full-text

Seed storage behaviour and germination characteristics of trees and shrubs of Hong Kong

10.5353/th_b3122287 ◽

1999 ◽

Author(s):

Wing-yee, Vicky Lam

Keyword(s):

Hong Kong ◽

Seed Storage ◽

Germination Characteristics ◽

Trees And Shrubs

Download Full-text

619 PB 325 Respiration as an indicator of health in excised, dormant vegetative apple buds subjected to cryopreservation treatments

HortScience ◽

10.21273/hortsci.29.5.521a ◽

1994 ◽

Vol 29 (5) ◽

pp. 521a-521

Author(s):

L.E. Towill ◽

S.A. Blackman

Keyword(s):

Moisture Content ◽

Low Temperatures ◽

Room Temperature ◽

Seed Storage ◽

Sublethal Injury ◽

The Status ◽

And Storage

cryopreservation of dormant, vegetative apple buds at the National Seed Storage Laboratory is used to maintain a base collection for germplasm held in the National Clonal Germplasm Repository for apple and grape, Geneva, NY, and is performed by a method previously reported1. Growth of buds after grafting is now used to test for survival after exposure to and storage at very low temperatures (ca.-160°C). We are interested in determining if measures of respiration can be used to assess 1. the status of buds and bark used for preservation, 2. survival after different treatments related to cryopreservation, and 3. the extent of sublethal injury after treatments. A Licor 6252 CO2 analyzer was used to measure respiration. Reproducible measurements of respiration required at least 2-3 buds. Buds from winter harvested twigs (ca 45-48% moisture content) that were briefly warmed to room temperature respired at a rate of 34 umoles CO2 g-1 (dw) hr-1. Survival of buds is enhanced if twigs are dried prior to cooling. We found such treatments reduced respiration over non-dried controls. Respiration increased as the bud was rehydrated. Buds from dried twigs slowly cooled to low temperatures had levels of respiration after warming and rehydrating similar to undried, unfrozen controls. Buds from undried twigs directly placed at -196°C and warmed gave little CO2 production.

Download Full-text

Comparison of Germination Characteristics and Daily Seed Germinating Pattern in Fine-textured Fescues Grown Under Alternative and Natural Conditions at the Room Temperature

Weed & Turfgrass Science ◽

10.5660/wts.2013.2.1.047 ◽

2013 ◽

Vol 2 (1) ◽

pp. 47-54 ◽

Cited By ~ 3

Author(s):

Kyoung-Nam Kim

Keyword(s):

Room Temperature ◽

Natural Conditions ◽

Germination Characteristics

Download Full-text

Seed Germination and Storage of the Endangered Species Manglietia crassipes Y. W. Law (Magnoliaceae)

Horticulturae ◽

10.3390/horticulturae7030042 ◽

2021 ◽

Vol 7 (3) ◽

pp. 42

Author(s):

Ai-Hua Wang ◽

Xiao-Ling Yu ◽

Yan-Yan Liu ◽

Shu-Gang Chen ◽

Fa-Guo Wang

Keyword(s):

Seed Germination ◽

Endangered Species ◽

Seed Dormancy ◽

Germination Rate ◽

Seed Storage ◽

Storage Conditions ◽

Seed Vigor ◽

Water Control ◽

Critically Endangered Species ◽

Germination Characteristics

Manglietia crassipes, a critically endangered species narrowly distributed on Mount Dayao in Guangxi, China, is also a species of ornamental interest whose variability has not been explored. Key factors leading to its endangerment have also not been studied. Here, two experiments were conducted to test the effects of different plant growth regulators and different storage conditions on germination characteristics of M. crassipes seeds. Fruit morphology was measured, and germination characteristics of fresh mature seeds were tested in order to assess natural seed vigor. Seeds were soaked in distilled water (control), or gibberellic acid (GA3), 6-benzylaminopurine (6-BA) or indoleacetic acid (IAA) solutions of different concentrations, for 48 h to determine their effects on seed germination. In addition, the effects of different seed storage conditions (constant 4 °C, −7 °C, −20 °C, or 25 °C for 100 days, wet stratification at 4 °C for 100 days) on seed germination were investigated. Results showed that the abortive rate of the mature fruits was high (28.9%) and fresh natural seeds had a low germination rate (G) and germination index (GI). Seed germination was inhibited with 6-BA at all concentrations, but only at less than 100 m·L−1 IAA; otherwise, IAA slightly promoted seed germination. GA3 broke seed dormancy and significantly accelerated seed germination by improving G, GI and initial germination time (IGT), especially over 2500 mg·L−1. The viability of seeds declined no matter how they were stored. However, 4 °C wet stratification storage was preferable for seed vigor and germination power. Our results suggest that the high abortive rate of fruits, low germination of the natural seed, seed dormancy and its intolerance to storage, contributed to the endangerment of M. crassipes. GA3 can break M. crassipes seed dormancy, which can be a benefit for future ornamental breeding and further protection or conservation.

Download Full-text

Viabilitas Benih Bawang Putih Varietas Tawang Mangu Setelah Penyimpanan pada Berbagai Suhu

Comm Horticulturae Journal ◽

10.29244/chj.2.3.43-47 ◽

2019 ◽

Vol 2 (3) ◽

pp. 43

Author(s):

. Sulassih ◽

Yohanes Aris Purwanto ◽

. Sobir ◽

Naekman Naibaho ◽

Siaga Yudha Pratama ◽

...

Keyword(s):

Weight Loss ◽

High Temperature ◽

Moisture Content ◽

Room Temperature ◽

Seed Quality ◽

Light Condition ◽

Seed Storage ◽

Physical Damage ◽

Small Weight ◽

Field Emergence

Viability of garlic seed is important for seed quality. Periode of seed storage Penyimpanan benih berkaitan dengan viabilitas benih. Viability testing measured after storage at 0 oC, 7 oC and room temperate. Moisture content decreased (40-45%) after 1 month in the storage under light condition, but the weight loss approximately at 1-8%, still lower than ASEAN rules (2009) that is not over than 10-15% for physical damage. Tawang Mangu variety showed higher for percentage of field emergence (83.33%) after was storages at 0 oC for one month and produced higher bulb (11 bulbs), but at the room temperature showed the lower for percentage of field emergence (56.94%) and produced 2-3 bulbs. All the treatment (7oC and room temperature) produced small size of bulbs in weight (1-2 g), height (1.5-1.7 cm) and width (1.5-1.6 cm). Three of the temperature condition was produced single bulb called “bawang lanang”, it suggested causing the land unfertilizer. Garlic was not suitable for medium land and high temperature such as Bogor.Keywords: diameter, height, male garlic, small, weight

Download Full-text

Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.2008.024 ◽

2011 ◽

Vol 77 (3) ◽

pp. 201-205 ◽

Cited By ~ 2

Author(s):

Agnieszka I. Piotrowicz-Cieślak ◽

Dariusz J. Michalczyk ◽

Kamila Górska ◽

Zofia Bulińska-Radomska ◽

Ryszard J. Górecki

Keyword(s):

Seed Coat ◽

Room Temperature ◽

Seed Storage ◽

Lupinus Luteus ◽

Soluble Carbohydrates ◽

Raffinose Family Oligosaccharides ◽

Seed Vigour ◽

Long Term Storage ◽

Different Temperatures ◽

Lupin Seeds

Seed vigour, viability, the contents of soluble carbohydrates, total protein, albumins, and globulins, as well as seed coat structure, were analysed in yellow lupin (Lupinus luteus L.) cv. Iryd seeds stored for 20 years at -14oC, 0oC or at room temperature (approx. +20oC). Seed storage at room temperature reduced viability (to 2%) and increased seed leachate electroconductivity. Determinations of total proteins showed that protein content was significantly reduced in seeds stored at +20oC compared to the other storage regimens. Raffinose family oligosaccharides were the main soluble carbohydrates in seeds stored at 0oC and -14oC, whereas sucrose dominated in seeds stored at room temperature. Scanning electron microscopy (SEM) of seed surface and seed coat sections revealed appearance of an amorphic layer on the surface of seeds stored at room temperature (not observed in other seeds) and distinct shrinking of macrosclereid layer in seeds stored at -14oC. Macrosclereids layer in all seeds was 100 um thick and accounted for 60% of seed coat thickness. The obtained results suggest that for long term storage of lupin seeds at 0oC is the most advisable temperature if both costs of storage and seed storability are considered.

Download Full-text

EFFECTS OF STORAGE CONDITIONS AND PRESENCE OF FRUITING BRACTS ON THE GERMINATION OF ATRIPLEX HALIMUS AND SALSOLA VERMICULATA

Experimental Agriculture ◽

10.1017/s0014479797000021 ◽

1997 ◽

Vol 33 (2) ◽

pp. 149-155 ◽

Cited By ~ 16

Author(s):

A. E. OSMAN ◽

F. GHASSALI

Keyword(s):

Seed Germination ◽

Cold Storage ◽

North Africa ◽

Room Temperature ◽

Storage Temperature ◽

Seed Storage ◽

Storage Conditions ◽

Shrub Species ◽

Atriplex Halimus ◽

Different Temperatures

Two shrub species, Atriplex halimus L. and Salsola vermiculata L., are considered useful for rehabilitation of degraded rangelands in west Asia and north Africa. They can be established from direct seeding and are capable of self-sowing. In this study, seed storage at different temperatures and the influence of fruiting bracts on seed germination were examined for the two species during two seasons. Fruits (utricles) were stored at 20–22°C (room temperature), 0°C or −22°C. Germination tests were carried out after 33, 56, 90, 152, 272 and 397 d in storage in the first season and after 44, 76, 104, 170, 288 and 412 d in the second season. Seeds were germinated in their fruiting bracts or after bract removal. Bract removal significantly improved seed germination of both shrubs regardless of storage temperature. For S. vermiculata the increase in germination was in the range of 1.3- to 14.7-fold compared with values for the intact fruit in Season 1 and 0.5 to 3.8 in Season 2. Similarly the ranges for A. halimus were 0.5- to 4.2-fold and 0.7- to 5.3-fold in the two seasons respectively. The effect of cold storage was greater on Salsola than on Atriplex. The reduction of the storage temperature from 21°C to 0°C and −22°C increased the longevity of S. vermiculata seeds by 2.8–46.6 times in Season 1 and by 2.9–2.6 times in Season 2. There was little or no effect on the longevity of A. halimus. A leachate prepared by soaking fruiting bracts from S. vermiculata significantly depressed germination (p < 0.01), the effect being greater on Salsola seeds (20% reduction) than on Atriplex seeds (8% reduction). A leachate from A. halimus produced a slight but non-significant reduction in germination.

Download Full-text