scholarly journals Peer Review #1 of "Transcriptomic response of maize primary roots to low temperatures at seedling emergence (v0.1)"

1978 ◽  
Vol 58 (2) ◽  
pp. 493-498 ◽  
Author(s):  
PRASANTA C. BHOWMIK

Germination percentage of common milkweed (Asclepias syriaca L.) seeds was low 1 mo after seed collection. Seed dormancy decreased with time at storage temperatures of −12°, 5° or 21 °C. After 11 months of storage, seeds stored at 21 °C had 15–18% higher germination compared to the seeds stored at −12° and 5 °C. The best seedling emergence was obtained at a temperature of 27 °C when seeds were planted at a depth of 0.5 or 1 cm. Seedling emergence was better in muck or sandy soil than in clay soil. Seedlings developed slowly up to 30 days after emergence at 15 °C under an 8-, 12- or 16-h photoperiod. High temperatures (27 °C) stimulated seedling growth under each photoperiod. Taller seedlings with more leaves, longer primary roots, more lateral roots and adventitious root buds grew at 27 °C as compared to 15° or 21 °C. Increasing the photoperiod from 8 to 16 h increased plant height and number of leaves but not primary root length.


HortScience ◽  
1993 ◽  
Vol 28 (9) ◽  
pp. 902-903 ◽  
Author(s):  
Jack Fry ◽  
Ward Upham ◽  
Larry Leuthold

Field studies were conducted in consecutive years to evaluate the influence of seeding month and seed soaking on buffalograss [Buchloë ductyloides (Nutt.) Engelm.] establishment, as measured by percentage of coverage and seedling emergence. In 1991, plots where `Sharp's Improved' buffalograss burrs were seeded in May, June, or July exhibited complete coverage 7 weeks after seeding (WAS). Between Apr. and Sept. 1992, mean high and low temperatures were ≈ 3C cooler than in 1991, and seeding in June or July resulted in >95% coverage 9 WAS. In the same year, seeding in April or May required 12 to 13 weeks for complete coverage. Buffalograss seeded in August exhibited <25% coverage by the end of the first growing season. Soaking buffalograss burrs in water before seeding resulted in the emergence of >30% more seedlings 2 WAS compared with nonsoaked burrs and increased coverage by up to 18% on selected rating dates 3 to 13 WAS. However, complete coverage occurred only ≈ week sooner where soaked vs. nonsoaked burrs were planted.


2020 ◽  
Vol 80 ◽  
pp. 13-33
Author(s):  
Parvin Salehi Shanjani ◽  
Amir Mousavi ◽  
Faeze Rasoulzadeh Farsad

Ferula assa-foetida and F. gummosa, Apiaceae, are important endemic and endangered medicinal plants. Survival of the species is threatened by climate change, overexploiting (as source of oleo-gum resin and for-age) and lack of organized cultivation. Cultivation of these valuable medicinal plants is restricted by insuffi-cient domestication knowledge. Germination characteristics of different populations of Ferula taxa were studied with the aim of describing and comparing their responses to continuous cold stratification condition. Germination cues for the species were complex, with dormancy mechanisms present to restrict germination until cold stratification are fulfilled. Results indicated that a period of 4 weeks of stratification is sufficient for germination of F. assa-foetida, but optimal germination of F. gummosa require stratification for periods of 8 weeks. Both species were able to germinate at very low temperatures (4°C). Within-taxon differences in dor-mancy breaking and seedling emergence may interpret as local adaptations. The continued regeneration and propagation of the species in the wild will depend on the temperature and moisture status of the soil during winter and the maintenance of conditions suitable for stratification for an appropriate length of time.


2017 ◽  
Vol 39 (4) ◽  
pp. 401-409 ◽  
Author(s):  
Marcelo do Nascimento Araujo ◽  
Marisol Ferraz ◽  
Fabiana Karla Araújo Américo ◽  
Fabrício Francisco Santos Silva ◽  
Bárbara França Dantas ◽  
...  

Abstract: The aim of this study was to evaluate the effects of storage conditions on the germination of A. cearensis seeds. The experimental design was completely randomized into split plots over time with four replicates. The storage conditions of the airtight containers in the refrigerator and laboratory, paper bags in the laboratory and liquid nitrogen were assessed for 27 months. In the laboratory, we evaluated the germination, the germination rate, uniformity of germination, and total soluble and reducing sugars in the radicle. In the greenhouse, we evaluated seedling emergence, emergence rate and height of 30-day-old seedlings. Seeds stored in the refrigerator maintained a high initial germination rate, which decreased from the 21st month. Seeds stored in paper bags in the laboratory showed low emergence and small seedlings. Total soluble sugars and reducing sugars were mobilized when the seeds were stored at low temperatures. Thus, it is not advisable to store A. cearensis seeds in a laboratory environment without airtight containers. A. cearensis seeds kept in a refrigerated environment maintained their viability for at least two years.


1974 ◽  
Vol 83 (3) ◽  
pp. 551-555 ◽  
Author(s):  
D. Gupta ◽  
I. Kovács

SummarySix opaque·2 (O2) inbred lines and the single, three-way and double-cross hybrids derived from them were compared for emergence at low temperatures with their analogous normal inbred lines and hybrids. The results demonstrate that there is an increasing difference in emergence between the o2 and the normal forms with an increase in the period of incubation, the o2 types being inferior, in general, to the normal.A treatment of seeds with fungicides like Quinolate V4X, TMTD–Lindan and Vitavax 75W before sowing improved the cold tolerance of both the o2 and normal analogues, but the o2 forms remained far below the level of the normal.


2016 ◽  
Author(s):  
Mauro Di Fenza ◽  
Bridget Hogg ◽  
Jim Grant ◽  
Susanne Barth

Background. Maize (Zea mays) is C 4 tropical cereal and its adaptation to temperate climates can be problematic due to low soil temperatures at early stages of establishment. Methods. We report on a physiological and transcriptomic experiment on twelve maize varieties from a chilling condition adapted gene pool which identified four genotypes with significant contrasting chilling tolerance.These four varieties were subject to microarray analysis to identify up and down regulated genes under chilling conditions. Results. Stress induced by low temperature in the varieties Picker,PR39B29, Fergus and Codisco was reflected only in the expression profiles of the varieties Picker and PR39B29. No significant changes in expression were observed in Fergus and Codisco upon chilling stress. The overall number of genes up and down regulated in the two chilling tolerant varieties amounted to69. These two varieties exhibited two different trancriptomic patterns in which only four genes were shared, although not all with the same degree of regulation. Overall the expression pattern was similar between the two chilling tolerant varieties, indicating a common response to chilling stress. Discussion. Varieties with an enhanced root/shoot growth ratio under low temperature were more tolerant which could be an early and inexpensive measure for germ plasm screening. We have identified novel cold inducible genes in an already adapted maize breeding gene pool. This illustrates that further varietal selection for enhanced chilling tolerance is possible in an already preselected gene pool.


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