Molecular biodiversity under ocean warming: proteomics and fitness data provide clues for a better understanding of thermal tolerance in fish

Ocean warming is known to alter the performance of marine organisms albeit the proteome underpinnings of thermal tolerance are still largely unknown. Following a 1-month exposure to elevated temperatures we assessed the vulnerability of the proteome in the sea bream Sparus aurata to ocean warming. Fish were exposed to 18°C (control), 24°C (nursery ground) and 30 °C (heat wave year 2100). Survival was impaired after 28 days, mainly at 30°C although fishes’ condition was unaltered. Protein expression profiles (assessed at 14 and 21 days) were similar between fish exposed to 18 and 24ºC, differing substantially from fish exposed to 30ºC. Fish subjected to 24ºC showed enhanced glycolysis and decreased glycogenolysis mainly at 14 days of exposure. Fish subjected to 30ºC also showed enhanced glycolysis and up-regulated proteins related to gene expression, cellular stress response (CSR), and homeostasis. However, inflammatory processes were elicited at 21 days along with a down-regulation of the tricarboxylic acid cycle. Thus, juvenile fish acclimated to 24 but not to 30ºC as a result of increasing physiological constraints associated with metabolic scope available for performance at higher temperatures. Consequently, recruitment of sea breams may be in jeopardy with potential effects on population persistence and distribution.

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Metformin counteracts glucose-dependent lipogenesis and impairs transdeamination in the liver of gilthead sea bream (Sparus aurata)

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00216.2018 ◽

2019 ◽

Vol 316 (3) ◽

pp. R265-R273 ◽

Cited By ~ 2

Author(s):

Ania Rashidpour ◽

Jonás I. Silva-Marrero ◽

Lidia Seguí ◽

Isabel V. Baanante ◽

Isidoro Metón

Keyword(s):

Molecular Mechanisms ◽

Sparus Aurata ◽

Current Knowledge ◽

Tricarboxylic Acid Cycle ◽

Intraperitoneal Administration ◽

Metabolic Effects ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Glucose Load ◽

Carnivorous Fish

Metformin is an antidiabetic drug with a major impact on regulating blood glucose levels by decreasing hepatic gluconeogenesis, but also by affecting other pathways, including glucose transport and energy/lipid metabolism. Carnivorous fish are considered glucose intolerant, as they exhibit poor ability in using dietary carbohydrates. To increase the current knowledge about the molecular mechanisms by which metformin can improve glucose homeostasis in carnivorous fish, we addressed the effect of intraperitoneal administration of metformin, in the presence or absence of a glucose load, on metabolic rate-limiting enzymes and lipogenic factors in the liver of gilthead sea bream ( Sparus aurata). Hyperglycemia markedly upregulated the expression of glycolytic enzymes (glucokinase and 6-phosphofructo-1-kinase, PFK1) 5 h following glucose administration, while at 24 h posttreatment, it increased isocitrate dehydrogenase (IDH) activity, a key enzyme of the tricarboxylic acid cycle, and the expression of lipogenic factors (PGC1β, Lpin1, and SREBP1). Metformin counteracted glucose-dependent effects, and downregulated glutamate dehydrogenase, alanine aminotransferase, and mammalian target of rapamycin 5 h posttreatment in the absence of a glucose load, leading to decreased long-term activity of PFK1 and IDH. The results of the present study suggest that hyperglycemia enhances lipogenesis in the liver of S. aurata and that metformin may exert specific metabolic effects in fish by decreasing hepatic transdeamination and suppressing the use of amino acids as gluconeogenic substrates. Our findings highlight the role of amino acid metabolism in the glucose-intolerant carnivorous fish model.

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Targets for TNF -induced lipolysis in gilthead sea bream (Sparus aurata L.) adipocytes isolated from lean and fat juvenile fish

Journal of Experimental Biology ◽

10.1242/jeb.029025 ◽

2009 ◽

Vol 212 (14) ◽

pp. 2254-2260 ◽

Cited By ~ 34

Author(s):

L. Cruz-Garcia ◽

A. Saera-Vila ◽

I. Navarro ◽

J. Calduch-Giner ◽

J. Perez-Sanchez

Keyword(s):

Sparus Aurata ◽

Juvenile Fish ◽

Gilthead Sea Bream ◽

Sea Bream

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Co-expression of IGFs and GH receptors (GHRs) in gilthead sea bream (Sparus aurata L.): sequence analysis of the GHR-flanking region

Journal of Endocrinology ◽

10.1677/joe-06-0229 ◽

2007 ◽

Vol 194 (2) ◽

pp. 361-372 ◽

Cited By ~ 26

Author(s):

Alfonso Saera-Vila ◽

Josep Alvar Calduch-Giner ◽

Jaume Pérez-Sánchez

Keyword(s):

Adipose Tissue ◽

Transcription Start Site ◽

Sparus Aurata ◽

Juvenile Fish ◽

Sea Bream ◽

Start Site ◽

Transcription Start ◽

Igf I ◽

Functional Partitioning ◽

Gh Receptors

The tissue-specific expression of IGFs and GH receptors (GHRs) was analyzed in gilthead sea bream (Sparus aurata L.) as an attempt to understand the functional partitioning of duplicated GHRs on the regulation offish growth by season and aging. Gene transcripts were measured in liver, muscle, and adipose tissue by means of quantitative real-time PCR assays. In juvenile fish, concurrent increases in circulating levels of GH and IGF-I and hepatic mRNA levels of IGF-I and GHR-I were evidenced with the summer growth spurt. Conversely, muscle and adipose tissue expression of GHR-I and IGF-II were significantly upregulated by over wintering. The aging decrease of growth rates was accompanied by a reduced activity of the liver GH/IGF axis, and parallel increases in muscle IGF expression would be dictated at the local tissue level by the enhanced expression of GHR-I. Extra-hepatic expression of IGFs and GHR-II did not correlate seasonally in juvenile fish, and nonsignificant effects of aging were found on the summer expression of GHR-II in any analyzed tissue. One transcription start site was identified by RLM-RACE in GHR-I and GHR-II. Sequence analyses indicated that both genes have TATA-less promoters containing consensus initiator sequences and downstream promoter elements surrounding the transcription start site. Conserved CCAAT-boxes and GC-rich regions were retrieved in the GHR-I promoter, whereas stress- and redox-sequence elements (cAMP-responsive element-binding protein, activator proteins; AP-1, and AP-4) were characteristic features of GHR-II. All this supports the functional partitioning of fish GHRs regardless of fish species differences.

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Sinop İli Su Ürünleri Üretiminin Mevcut Durumu ve Türkiye Su Ürünleri Üretimindeki Yeri

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v9isp.2437-2441.4844 ◽

2022 ◽

Vol 9 (sp) ◽

pp. 2437-2441

Author(s):

Şennan Yücel ◽

Birol Baki ◽

Tuçe Altın ◽

Gülşen Uzun Gören

Keyword(s):

Sparus Aurata ◽

Juvenile Fish ◽

Dicentrarchus Labrax ◽

Sea Bream ◽

Horse Mackerel ◽

The Black Sea ◽

Trachurus Trachurus ◽

Pomatomus Saltatrix ◽

Black Sea Region ◽

Engraulis Encrasicolus

In the present study, the production amount in the last five years (2015-2019) of Sinop province, which is located in the Black Sea Region and a significant part of fisheries production is carried out, was evaluated. For this purpose; Sinop Provincial Directorate of Agriculture and Forestry and TUIK (Turkish Statistical Institute) data were used. There are 2.326 commercial fishermen and 444 fishing boats in Sinop province. In the sea area of Sinop, 5 enterprises still produce culture fish in cages. The 2 potential marine culture areas identified in Sinop have an annual production capacity of 32.430 tons (31 enterprises). In addition, 5 bivalves (4.940 tons/year) and 1.000.000 number/year capacity juvenile fish production facilities are at the project stage. The total fisheries production of Sinop Province in 2019 was 34.620,9 tons, and 1.505 tons were obtained from aquaculture. In addition to these, 3.924 tons of fish processing facilities per year, and 35 tons of fish meal and oil processing facilities per year are operated. 66% of sea fish caught in Sinop are anchovy (Engraulis encrasicolus ponticus), 16% sprat (Sprattus sprattus phalericus), 13% horse mackerel (Trachurus trachurus), 3% bluefish (Pomatomus saltatrix) and 2% consists of other species. The most preferred marine fish by the consumers are anchovy (Engraulis encrasicolus ponticus), horse mackerel (Trachurus trachurus), whiting (Merlangus merlangus), red mullet (Mullus barbatus), bluefish (Pomatomus saltatrix), turbot (Psetta maxima) and bonito (Sarda sarda) and sea bream (Sparus aurata), sea bass (Dicentrarchus labrax) and rainbow trout (Oncorhyncus mykiss) are the culture fish species offered for sale throughout the year.

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