White spot and taura syndrome virus: disease as drivers in the shrimp farming industry

2012 ◽  
pp. 429-442
Author(s):  
David R.W. Griffith
2019 ◽  
Vol 6 (2) ◽  
pp. 48-63
Author(s):  
Truong Quoc Bao ◽  
Tran Chi Cuong ◽  
Nguyen Dinh Tu ◽  
Le Hoang Dang ◽  
Luu Trong Hieu

One of the most serious problems confronted by the shrimp farming industry is the disease caused by the yellow head virus (YHV). This research proposes an image processing algorithm to detect, identify and eliminate shrimp with the yellow head virus from the Litopenaeus vannamei gathering lines. Using a Raspberry Pi 3 module with the support of the OpenCV library which may be associated with Niblack’s algorithm is primarily suitable for segmentation. First, the shrimp object was identified and separated from the background using the image segmentation technique and the boundary that surrounds the object. Then, identification of diseased shrimp was analysed based on colour threshold. In this study, the sample of shrimp disease group had the highest amount of ratio, with about 6% to 11%. Most of the samples without the disease had a ratio of 0%. The experimental results show that the system can identify and accurately determine the coordinates of shrimp with yellow head virus disease and send information to the shrimp classification system in the food industry.


2004 ◽  
Vol 78 (4) ◽  
pp. 2057-2061 ◽  
Author(s):  
Jeroen Witteveldt ◽  
Carolina C. Cifuentes ◽  
Just M. Vlak ◽  
Mariëlle C. W. van Hulten

ABSTRACT White spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. No adequate treatments against this virus are available. It is generally accepted that invertebrates such as shrimp do not have an adaptive immune response system such as that present in vertebrates. As it has been demonstrated that shrimp surviving a WSSV infection have higher survival rates upon subsequent rechallenge, we investigated the potential of oral vaccination of shrimp with subunit vaccines consisting of WSSV virion envelope proteins. Penaeus monodon shrimp were fed food pellets coated with inactivated bacteria overexpressing two WSSV envelope proteins, VP19 and VP28. Vaccination with VP28 showed a significant lower cumulative mortality compared to vaccination with bacteria expressing the empty vectors after challenge via immersion (relative survival, 61%), while vaccination with VP19 provided no protection. To determine the onset and duration of protection, challenges were subsequently performed 3, 7, and 21 days after vaccination. A significantly higher survival was observed both 3 and 7 days postvaccination (relative survival, 64% and 77%, respectively), but the protection was reduced 21 days after the vaccination (relative survival, 29%). This suggests that contrary to current assumptions that invertebrates do not have a true adaptive immune system, a specific immune response and protection can be induced in P. monodon. These experiments open up new ways to benefit the WSSV-hampered shrimp farming industry.


2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Libia Zulema Rodriguez-Anaya ◽  
Jose Reyes Gonzalez-Galaviz ◽  
Ramón Casillas-Hernandez ◽  
Fernando Lares-Villa ◽  
Karel Estrada ◽  
...  

The first genome sequence of a Mexican white spot syndrome virus is presented here. White spot syndrome is a shrimp pandemic virus that has devastated production in Mexico for more than 10 years. The availability of this genome will greatly aid epidemiological studies worldwide, contributing to the molecular diagnostic and disease prevention in shrimp farming.


2019 ◽  
Vol 4 (1) ◽  
pp. 65
Author(s):  
Hari Suprapto, Yulia Kartika

Abstract The disease most dangerous for the cultivation activity is virus. Viruses are organisms subseluler that contain only nucleic acid (RNA or DNA) as genetic material. Koi Herpes Virus is one type of virus that causes mortality in cultured Cyprinids. KHV disease in Indonesia started in Blitar, East Java on March 2002 because the entry of imported koi fish that carry the virus KHV, while mortality prosentase could reach 80% - 85%, which causes loss of about 5 billion rupiah. In addition of KHV, there are several types of viral diseases in shrimp is White Spot Syndrome Virus (WSSV), Taura Syndrome Virus (TSV), dan Yellow Head Virus (YHV). Disease can cause losses in farming activities, such as WSSV. WSSV is an endemic disease since 1995. disease WSSV is exotic viral disease that attacks the shrimp monodon in 1998/1999 has resulted in decreased production of very large, so the Indonesian shrimp exports down 33,000 tons. Treatment of viral diseases is difficult because the virus resistant to certain antibiotics and chemical compounds. Therefore, prevention needs to be done, one through the monitoring activities conducted on the northern coast of East Java. The method implemented is monitoring in location and identification of viruses by PCR (Polymerase Chain Reaction). Monitoring in location includes water quality measurements and sampling. Identification of virus carried by IQ 2000TM. The identification procedure includes extraction, amplification and electrophoresis. Regional monitoring conducted on the northern coast of East Java includes Gresik, Lamongan, Tuban, Bangkalan, Sampang, Pamekasan, and Sumenep. Water quality at locations quite well. Results activities of monitoring on the northern coast of East Java is disease White Spot Syndrome Virus (WSSV) was found positive in several locations: Gresik, Lamongan and Tuban, while the virus Taura Syndrome Virus (TSV) and Yellow Head Virus (YHV) was not found at all locations . In tilapia, disease Koi Herpes Virus (KHV) was found positive in Tuban.


2016 ◽  
Vol 51 (Special-issue) ◽  
pp. S60-S65 ◽  
Author(s):  
Partho Pratim Debnath ◽  
Manjurul Karim ◽  
Hendrik Jan Keus ◽  
Chadag Vishnumurthy Mohan ◽  
Ben Belton

2020 ◽  
Vol 21 (4) ◽  
pp. 1243
Author(s):  
Heqian Zhang ◽  
Wenzhi Cheng ◽  
Jinbin Zheng ◽  
Panpan Wang ◽  
Qinghui Liu ◽  
...  

Kuruma prawn, Marsupenaeus japonicus, has the third largest annual yield among shrimp species with vital economic significance in China. White spot syndrome virus (WSSV) is a great threat to the global shrimp farming industry and results in high mortality. Pellino, a highly conserved E3 ubiquitin ligase, has been found to be an important modulator of the Toll-like receptor (TLR) signaling pathways that participate in the innate immune response and ubiquitination. In the present study, the Pellino gene from Marsupenaeus japonicus was identified. A qRT-PCR assay showed the presence of MjPellino in all the tested tissues and revealed that the transcript level of this gene was significantly upregulated in both the gills and hemocytes after challenge with WSSV and Vibrio parahaemolyticus. The function of MjPellino was further verified at the protein level. The results of the three-dimensional modeling and protein–protein docking analyses and a GST pull-down assay revealed that the MjPellino protein was able to bind to the WSSV envelope protein VP26. In addition, the knockdown of MjPellino in vivo significantly decreased the expression of MjAMPs. These results suggest that MjPellino might play an important role in the immune response of kuruma prawn.


Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 741 ◽  
Author(s):  
Ying Zou ◽  
Guosi Xie ◽  
Tianchang Jia ◽  
Tingting Xu ◽  
Chong Wang ◽  
...  

A new emerging disease called “translucent post-larvae disease” (TPD) or “glass post-larvae disease” (GPD) of Penaeus vannamei, characterized by pale or colorless hepatopancreas and digestive tract, has become an urgent threat to the shrimp farming industry. Following this clue that treatment of an antibacterial agent could alleviate the disease, systematic investigation of the potential infectious agent of TPD was conducted using bacterial identification and artificial challenge tests to fulfill Koch’s postulates. A dominant bacterial isolate, Vp-JS20200428004-2, from the moribund individuals was isolated and identified as Vibrio parahaemolyticus based on multi-locus sequence analysis. However, Vp-JS20200428004-2 differed from the V. parahaemolyticus that caused typical acute hepatopancreatic necrosis disease. Immersion challenge tests revealed that Vp-JS20200428004-2 could cause 100% mortality within 40 h at a dose of 1.83 × 106 CFU/mL, and experimental infected shrimp showed similar clinical signs of TPD. The Vp-JS20200428004-2 could be re-isolated and identified from the experimental infected individuals. Moreover, histopathological analysis of diseased samples indicated that Vp-JS20200428004-2 caused severe necrosis and sloughing of epithelial cells of the hepatopancreas and midgut in shrimp individuals both naturally and experimentally infected. Our present results indicated that Vp-JS20200428004-2 is a highly virulent infectious agent associated with the TPD and deserves further attention.


In any aquaculture business, sustainability of a system improved profits. At present although biosecurity and BAqP are in place, more needed to be done. With emerging disease challenges innovated designs and operation systems are developing for sustainable production. One of the most important factors the investors, shrimp farmers and technicians need to be aware of is that whatever waste discharged into environment will come back to you in a form of disease sooner or later. Before mid1990s major threats to shrimp farming was mainly bacterial diseases. In Asia from late 1994 appearance of viral diseases such as white spot syndrome virus (WSSV) and a few others like yellow head virus (YHV), infectious myonecrosis virus (IMNV). In 2001 with availability of Specific Pathogen Free (SPF) Penaeus vannamei broodstock from Hawaii, the shrimp farming industry took off much faster.


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