scholarly journals Sustainable Shrimp Farming: Biosecure Systems to Prevent or Control Emerging Diseases

2020 ◽  
Vol 3 (3) ◽  
Keyword(s):  
White Spot Syndrome Virus ◽  
Emerging Diseases ◽  
Shrimp Farming ◽  
Penaeus Vannamei ◽  
Business Sustainability ◽  
Bacterial Diseases ◽  
Yellow Head Virus ◽  
Infectious Myonecrosis Virus ◽  
Specific Pathogen ◽  
Farming Industry

In any aquaculture business, sustainability of a system improved profits. At present although biosecurity and BAqP are in place, more needed to be done. With emerging disease challenges innovated designs and operation systems are developing for sustainable production. One of the most important factors the investors, shrimp farmers and technicians need to be aware of is that whatever waste discharged into environment will come back to you in a form of disease sooner or later. Before mid1990s major threats to shrimp farming was mainly bacterial diseases. In Asia from late 1994 appearance of viral diseases such as white spot syndrome virus (WSSV) and a few others like yellow head virus (YHV), infectious myonecrosis virus (IMNV). In 2001 with availability of Specific Pathogen Free (SPF) Penaeus vannamei broodstock from Hawaii, the shrimp farming industry took off much faster.

scholarly journals Determination of the Infectious Agent of Translucent Post-Larva Disease (TPD) in Penaeus vannamei

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 741 ◽  
Author(s):  
Ying Zou ◽  
Guosi Xie ◽  
Tianchang Jia ◽  
Tingting Xu ◽  
Chong Wang ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Infectious Agent ◽  
Systematic Investigation ◽  
Shrimp Farming ◽  
Histopathological Analysis ◽  
Penaeus Vannamei ◽  
Challenge Tests ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Farming Industry

A new emerging disease called “translucent post-larvae disease” (TPD) or “glass post-larvae disease” (GPD) of Penaeus vannamei, characterized by pale or colorless hepatopancreas and digestive tract, has become an urgent threat to the shrimp farming industry. Following this clue that treatment of an antibacterial agent could alleviate the disease, systematic investigation of the potential infectious agent of TPD was conducted using bacterial identification and artificial challenge tests to fulfill Koch’s postulates. A dominant bacterial isolate, Vp-JS20200428004-2, from the moribund individuals was isolated and identified as Vibrio parahaemolyticus based on multi-locus sequence analysis. However, Vp-JS20200428004-2 differed from the V. parahaemolyticus that caused typical acute hepatopancreatic necrosis disease. Immersion challenge tests revealed that Vp-JS20200428004-2 could cause 100% mortality within 40 h at a dose of 1.83 × 106 CFU/mL, and experimental infected shrimp showed similar clinical signs of TPD. The Vp-JS20200428004-2 could be re-isolated and identified from the experimental infected individuals. Moreover, histopathological analysis of diseased samples indicated that Vp-JS20200428004-2 caused severe necrosis and sloughing of epithelial cells of the hepatopancreas and midgut in shrimp individuals both naturally and experimentally infected. Our present results indicated that Vp-JS20200428004-2 is a highly virulent infectious agent associated with the TPD and deserves further attention.

scholarly journals Diseases of shrimps and natural methods to protect their health

2019 ◽  
Vol 75 (01) ◽  
pp. 6154-2019 ◽  
Author(s):  
BARBARA KAZUŃ ◽  
KRZYSZTOF KAZUŃ ◽  
ANDRZEJ KRZYSZTOF SIWICKI
Keyword(s):  
Positive Impact ◽  
Animal Health ◽  
Shrimp Farming ◽  
Economic Losses ◽  
Viral Diseases ◽  
Human Consumption ◽  
Virus Genotype ◽  
White Spot Disease ◽  
Bacterial Diseases ◽  
Yellow Head Virus

Shrimp farming is an economically important part of aquaculture. Shrimps constitute the largest portion of shellfish intended for human consumption. According to the World Organization for Animal Health (OIE), viral diseases pose the most serious threat to shrimp farming. There are currently six viral and two bacterial diseases on the OIE list. The most serious viral diseases are white spot disease (WSD), Taura syndrome (TS), infection with yellow head virus genotype 1 (YHV), infectious hypodermal and haematopoietic necrosis (IHHN), infectious myonecrosis (IMN) and white tail disease (WTD), whereas the most dangerous bacterial diseases are acute hepatopancreatic necrosis disease (AHPND) and necrotizing hepatopancreatitis (NHP). All these diseases result in high mortality, thus limiting shrimp production and causing large economic losses. Therefore, effective preparations are constantly sought to control infectious diseases in shrimps. Probiotics and immunostimulants, thanks to their demonstrated antibacterial and antiviral effects, as well their positive impact on the immune system, may play a crucial role in protecting the health of shrimps.

scholarly journals ​​Nursery Rearing of Penaeus vannamei in Biofloc Systems with Different Salinities and Organic Carbon Sources

2022 ◽  
Author(s):  
T. Anand ◽  
A. Srinivasan ◽  
P. Padmavathy ◽  
P. Jawahar ◽  
J. Stephen Sampathkumar
Keyword(s):  
Present Experiment ◽  
Carbon Sources ◽  
Shrimp Farming ◽  
Penaeus Vannamei ◽  
Nitrogenous Waste ◽  
Significant Difference ◽  
Farming Industry ◽  
Waste Accumulation ◽  
Average Body ◽  
Lower Production

Background: Nursery rearing of Penaeus vannamei became inevitable in the Indian shrimp farming industry, since intensification of culture practices in grow-out systems caused nitrogenous wastes accumulation, diseases, mortality and premature harvests resulted in high food conversion ratio, lower production and profits. The nursery rearing in traditional water exchange systems often getting failure because of nitrogenous waste accumulation. Hence, the present experiment was planned to rear P. vannamei in bioflocs systems (BFS) with different salinities and carbon sources. Methods: The experiment was conducted in 0.22 m3 tanks with three different salinity groups viz., 35 ppt, 20 ppt and 5 ppt and in each salinity sugar, molasses used as carbon sources to maintain an estimated C/N ratio of 15:1 and controls without carbon sources. Experimental tanks were stocked @6 post larvae/l, with pre-salinity acclimatized P.vannamei seeds having 0.0029±0.0003g size and nursed for a period of 5 weeks. Result: The nitrogenous waste accumulation was reduced significantly (p less than 0.05), also average body weight and survival rate of the seeds showed significant difference (p less than 0.001) between treatments and controls and within treatments (p less than 0.05). Salinity, carbon sources and their interaction influenced the growth characteristics significantly (p less than 0.01). The present experiment manifested promising results of bioflocs nurseries in rearing of P. vannamei seeds at different salinities.

scholarly journals Designing the Yellow Head Virus Syndrome Recognition Application for Shrimp on an Embedded System

2019 ◽  
Vol 6 (2) ◽  
pp. 48-63
Author(s):  
Truong Quoc Bao ◽  
Tran Chi Cuong ◽  
Nguyen Dinh Tu ◽  
Le Hoang Dang ◽  
Luu Trong Hieu
Keyword(s):  
Image Processing ◽  
Embedded System ◽  
Food Industry ◽  
Virus Disease ◽  
Shrimp Farming ◽  
Raspberry Pi ◽  
Disease Group ◽  
Image Processing Algorithm ◽  
Yellow Head Virus ◽  
Farming Industry

One of the most serious problems confronted by the shrimp farming industry is the disease caused by the yellow head virus (YHV). This research proposes an image processing algorithm to detect, identify and eliminate shrimp with the yellow head virus from the Litopenaeus vannamei gathering lines. Using a Raspberry Pi 3 module with the support of the OpenCV library which may be associated with Niblack’s algorithm is primarily suitable for segmentation. First, the shrimp object was identified and separated from the background using the image segmentation technique and the boundary that surrounds the object. Then, identification of diseased shrimp was analysed based on colour threshold. In this study, the sample of shrimp disease group had the highest amount of ratio, with about 6% to 11%. Most of the samples without the disease had a ratio of 0%. The experimental results show that the system can identify and accurately determine the coordinates of shrimp with yellow head virus disease and send information to the shrimp classification system in the food industry.

scholarly journals Protection of Penaeus monodon against White Spot Syndrome Virus by Oral Vaccination

2004 ◽  
Vol 78 (4) ◽  
pp. 2057-2061 ◽  
Author(s):  
Jeroen Witteveldt ◽  
Carolina C. Cifuentes ◽  
Just M. Vlak ◽  
Mariëlle C. W. van Hulten
Keyword(s):  
Immune Response ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
Relative Survival ◽  
Envelope Proteins ◽  
Shrimp Farming ◽  
White Spot ◽  
Oral Vaccination ◽  
Adaptive Immune ◽  
Farming Industry

ABSTRACT White spot syndrome virus (WSSV) occurs worldwide and causes high mortality and considerable economic damage to the shrimp farming industry. No adequate treatments against this virus are available. It is generally accepted that invertebrates such as shrimp do not have an adaptive immune response system such as that present in vertebrates. As it has been demonstrated that shrimp surviving a WSSV infection have higher survival rates upon subsequent rechallenge, we investigated the potential of oral vaccination of shrimp with subunit vaccines consisting of WSSV virion envelope proteins. Penaeus monodon shrimp were fed food pellets coated with inactivated bacteria overexpressing two WSSV envelope proteins, VP19 and VP28. Vaccination with VP28 showed a significant lower cumulative mortality compared to vaccination with bacteria expressing the empty vectors after challenge via immersion (relative survival, 61%), while vaccination with VP19 provided no protection. To determine the onset and duration of protection, challenges were subsequently performed 3, 7, and 21 days after vaccination. A significantly higher survival was observed both 3 and 7 days postvaccination (relative survival, 64% and 77%, respectively), but the protection was reduced 21 days after the vaccination (relative survival, 29%). This suggests that contrary to current assumptions that invertebrates do not have a true adaptive immune system, a specific immune response and protection can be induced in P. monodon. These experiments open up new ways to benefit the WSSV-hampered shrimp farming industry.

scholarly journals The Complete Genome of an Endogenous Nimavirus (Nimav-1_LVa) From the Pacific Whiteleg Shrimp Penaeus (Litopenaeus) Vannamei

Genes ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 94
Author(s):  
Weidong Bao ◽  
Kathy Tang ◽  
Acacia Alcivar-Warren
Keyword(s):  
Litopenaeus Vannamei ◽  
White Spot Syndrome Virus ◽  
Shrimp Farming ◽  
Endogenous Virus ◽  
Free Living ◽  
Host Interactions ◽  
The Pacific ◽  
The Family ◽  
Farming Industry ◽  
Host Genes

White spot syndrome virus (WSSV), the lone virus of the genus Whispovirus under the family Nimaviridae, is one of the most devastating viruses affecting the shrimp farming industry. Knowledge about this virus, in particular, its evolution history, has been limited, partly due to its large genome and the lack of other closely related free-living viruses for comparative studies. In this study, we reconstructed a full-length endogenous nimavirus consensus genome, Nimav-1_LVa (279,905 bp), in the genome sequence of Penaeus (Litopenaeus) vannamei breed Kehai No. 1 (ASM378908v1). This endogenous virus seemed to insert exclusively into the telomeric pentanucleotide microsatellite (TAACC/GGTTA)n. It encoded 117 putative genes, with some containing introns, such as g012 (inhibitor of apoptosis, IAP), g046 (crustacean hyperglycemic hormone, CHH), g155 (innexin), g158 (Bax inhibitor 1 like). More than a dozen Nimav-1_LVa genes are involved in the pathogen-host interactions. We hypothesized that g046, g155, g158, and g227 (semaphorin 1A like) were recruited host genes for their roles in immune regulation. Sequence analysis indicated that a total of 43 WSSV genes belonged to the ancestral/core nimavirus gene set, including four genes reported in this study: wsv112 (dUTPase), wsv206, wsv226, and wsv308 (nucleocapsid protein). The availability of the Nimav-1_LVa sequence would help understand the genetic diversity, epidemiology, evolution, and virulence of WSSV.

scholarly journals The Honeybee Gut Mycobiota Cluster by Season Versus the Microbiota Which Cluster by Gut Segment

2020 ◽  
Vol 8 (1) ◽  
pp. 4
Author(s):  
Jane Ludvigsen ◽  
Åsmund Andersen ◽  
Linda Hjeljord ◽  
Knut Rudi
Keyword(s):  
Emerging Diseases ◽  
Insect Species ◽  
Current Work ◽  
Bacterial Diseases ◽  
Honeybee Health

Honeybees represent one of the most important insect species we have, particularly due to their pollinating services. Several emerging fungal and bacterial diseases, however, are currently threatening honeybees without known mechanisms of pathogenicity. Therefore, the aim of the current work was to investigate the seasonal (winter, spring, summer, and autumn) fungal and bacterial distribution through different gut segments (crop, midgut, ileum, and rectum). This was done from two hives in Norway. Our main finding was that bacteria clustered by gut segments, while fungi were clustered by season. This knowledge can therefore be important in studying the epidemiology and potential mechanisms of emerging diseases in honeybees, and also serve as a baseline for understanding honeybee health.

scholarly journals The effect of salinity on enterocytozoon hepatopenaei infection in Penaeus vannamei under experimental conditions

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
L. F. Aranguren Caro ◽  
F. Alghamdi ◽  
K. De Belder ◽  
J. Lin ◽  
H. N. Mai ◽  
...  
Keyword(s):  
Penaeus Monodon ◽  
Oral Route ◽  
Western Hemisphere ◽  
Penaeus Vannamei ◽  
Asian Countries ◽  
Experimental Conditions ◽  
Reliable Source ◽  
Specific Pathogen ◽  
Enterocytozoon Hepatopenaei ◽  
First Time

Abstract Background Enterocytozoon hepatopenaei (EHP) is an enteric pathogen that affects Penaeus vannamei and Penaeus monodon shrimp in many SE Asian countries. In the western hemisphere, EHP was reported for the first time in 2016 in farmed P. vannamei in Venezuela. Anecdotal evidence suggests that EHP is more prevalent in grow-out ponds where the salinity is high (> 15 parts per thousand (ppt)) compared to grow-out ponds with low salinities (< 5 ppt). Considering that P. vannamei is an euryhaline species, we were interested in knowing if EHP can propagate in P. vannamei in low salinities. Results In this study, we described an experimental infection using fecal strings as a source inoculum. Specific Pathogen Free (SPF) P. vannamei were maintained at three different salinities (2 ppt, 15 ppt, and 30 ppt) while continuously challenged using feces from known EHP-infected P. vannamei over a period of 3 weeks. The fecal strings, used as a source of EHP inocula in the challenges, was sufficient to elicit an infection in shrimp maintained at the three salinities. The infectivity of EHP in shrimp reared at 2 ppt, 15 ppt, and 30 ppt salinities was confirmed by PCR and histopathology. The prevalence and the severity of the EHP infection was higher at 30 ppt than at 2 ppt and 15 ppt. Conclusion The data suggests that fecal strings are a reliable source of EHP inoculum to conduct experimental challenges via the fecal-oral route. An EHP infection can occur at a salinity as low as 2 ppt, however, the prevalence and the severity of the EHP infection is higher at a salinity of 30 ppt.

scholarly journals Draft Genome Sequence of White Spot Syndrome Virus Isolated from Cultured Litopenaeus vannamei in Mexico

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Libia Zulema Rodriguez-Anaya ◽  
Jose Reyes Gonzalez-Galaviz ◽  
Ramón Casillas-Hernandez ◽  
Fernando Lares-Villa ◽  
Karel Estrada ◽  
...  
Keyword(s):  
Disease Prevention ◽  
Genome Sequence ◽  
Litopenaeus Vannamei ◽  
White Spot Syndrome Virus ◽  
Draft Genome ◽  
Epidemiological Studies ◽  
Shrimp Farming ◽  
White Spot ◽  
Molecular Diagnostic ◽  
White Spot Syndrome

The first genome sequence of a Mexican white spot syndrome virus is presented here. White spot syndrome is a shrimp pandemic virus that has devastated production in Mexico for more than 10 years. The availability of this genome will greatly aid epidemiological studies worldwide, contributing to the molecular diagnostic and disease prevention in shrimp farming.

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