scholarly journals Celecoxib, a COX-2 Selective Inhibitor, Induces Cell Cycle Arrest at the G2/M Phase in HeLa Cervical Cancer Cells

2016 ◽  
Vol 17 (4) ◽  
pp. 1655-1659 ◽  
Author(s):  
Agustina Setiawati
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Selective Inhibitor ◽  
Cycle Arrest ◽  
M Phase ◽  
Cervical Cancer Cells ◽  
Cox 2

Resveratrol analog, N-(4-methoxyphenyl)-3,5-dimethoxybenzamide induces G2/M phase cell cycle arrest and apoptosis in HeLa human cervical cancer cells

2019 ◽  
Vol 124 ◽  
pp. 101-111 ◽  
Author(s):  
Kyung-Won Lee ◽  
Kyung-Sook Chung ◽  
Jeong-Hun Lee ◽  
Jung-Hye Choi ◽  
Sang Yoon Choi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Phase Cell ◽  
Cycle Arrest ◽  
M Phase ◽  
Cervical Cancer Cells ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

scholarly journals Anticancer activity of isomultiflorenol against human cervical cancer cells due to G2/M cell cycle arrest, autophagy and mitochondrial mediated apoptosis

2020 ◽  
Vol 19 (7) ◽  
pp. 1423-1428
Author(s):  
Juan Li ◽  
Yuanyuan Chen
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Hela Cells ◽  
Cycle Arrest ◽  
M Phase ◽  
Cervical Cancer Cells ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

Purpose: To determine the anticancer effect of a pentacyclic triterpenoid, isomultiflorenol, against human cervical cancer.Methods: The proliferation of cancer cells was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. Cell viability was measured with colony forming assay, while flow cytometry was used to study phase distribution in cancer cell mitosis. Electron microscopy was employed for the determination of autophagy induction in the cancer cells, while western blotting was used to assay protein expressions.Results: Isomultiflorenol significantly (p < 0.05) inhibited the proliferation and viability of cervical cancer cells in a concentration-dependent manner. The IC50 of isomultiflorenol was 10 μM for HeLa cells, and 90 μM for normal EV304 cells. The anti-proliferative effects were exerted as a result of arrest of HeLa cells at G2/M phase. The G2/M phase cells increased from 10.34 % in control to 30.21 % on treatment with 20 μM isomultiflorenol. Furthermore, administration of isomultiflorenol led to induction of cancer cell autophagy via mitochondrial apoptotic signaling.Conclusion: Isomultiflorenol inhibits human cervical cancer cells in vitro by inducing cell cycle arrest and autophagy. Thus, it is a potential lead molecule in the development of cervical cancer chemotherapy. Keywords: Cervical cancer, Terpenoids, Isomultiflorenol, Autophagy, Cell cycle arrest, Apoptosis

scholarly journals Buformin increases radiosensitivity in cervical cancer cells via cell-cycle arrest and delayed DNA-damage repair

2018 ◽  
Vol 243 (14) ◽  
pp. 1133-1140
Author(s):  
Ling Chen ◽  
Ting Zhang ◽  
Qiuli Liu ◽  
Mei Tang ◽  
Yu’e Yang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Clinical Treatment ◽  
Phase Cell ◽  
Antitumor Effects ◽  
Cycle Arrest ◽  
Cervical Cancer Cells ◽  
Old Drugs

Buformin is a commonly used hypoglycemic agent, and numerous studies have shown that buformin has potent antitumor effects in several malignancies. In this study, we aimed to assess the cytotoxic effect of buformin combined with ionizing radiation (IR) on two human cervical cancer cell lines (Hela and Siha). Cytotoxicity was detected by colony formation assays; impacts on the cell cycle and apoptosis were detected by flow cytometric analyses. Changes in histone H2AX (γ-H2AX) phosphorylation and impacts on the AMPK/S6 pathway were also explored. Our data show that the combination of buformin and IR had a much stronger antiproliferative effect and resulted in more apoptosis than did buformin or IR alone. Combination treatment with a low dose of buformin (10 µM) and IR (4 Gy) caused G2/M-phase cell cycle arrest. Consistent with these findings, Western blotting showed that the combination of buformin and IR activated AMPK and suppressed S6. In addition, delayed disappearance of γ-H2AX was detected by immunofluorescence in cervical cancer cells treated with buformin plus IR. Taken together, the data indicate that the combination of a low concentration of buformin and IR increases the radiosensitivity of cervical cancer cells via cell cycle arrest and inhibition of DNA repair. Based on these results, we strongly support the use of buformin as an effective agent for improving IR treatment efficiency in the context of cervical cancer. Impact statement Our idea originated in the thought of discovering new effects of old drugs. Although this study is a basic research, it is very close to clinical treatment. Flow cytometry and immunofluorescence were used to verify that buformin increases radiosensitivity. We aimed to address one of the thorniest problems in treatment process. Based on discovering new effects of old drugs, it is feasible to use buformin as an anticancer drug in clinical application. This will provide new ideas for clinical treatment.

scholarly journals The anticancer effects of ferulic acid is associated with induction of cell cycle arrest and autophagy in cervical cancer cells

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Jinhua Gao ◽  
Hui Yu ◽  
Weikang Guo ◽  
Ying Kong ◽  
lina Gu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Ferulic Acid ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Cycle Arrest ◽  
Anticancer Effects ◽  
Cervical Cancer Cells

scholarly journals SH003-induced G1 phase cell cycle arrest induces apoptosis in HeLa cervical cancer cells

2017 ◽  
Vol 16 (6) ◽  
pp. 8237-8244 ◽  
Author(s):  
Kang Min Lee ◽  
Kangwook Lee ◽  
Youn Kyung Choi ◽  
Yu-Jeong Choi ◽  
Hye-Sook Seo ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Phase Cell ◽  
G1 Phase ◽  
Cycle Arrest ◽  
Cervical Cancer Cells

scholarly journals 1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione Induces G1 Cell Cycle Arrest and Autophagy in HeLa Cervical Cancer Cells

2016 ◽  
Vol 17 (8) ◽  
pp. 1274 ◽  
Author(s):  
Jie-Heng Tsai ◽  
Li-Sung Hsu ◽  
Hsiu-Chen Huang ◽  
Chih-Li Lin ◽  
Min-Hsiung Pan ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Cycle Arrest ◽  
Cervical Cancer Cells ◽  
G1 Cell Cycle Arrest
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