ROLE OF OCIMUM CANUM IN PREVENTION OF REPERFUSION-INDUCED RENAL ISCHEMIA IN WISTAR ALBINO RATS

Aim: In cyclosporin-A (CsA)-induced toxicity, oxidative stress has been implicated as a potential responsible mechanism. Therefore, we aimed to investigate the protective role of erdosteine against CsA-induced nephrotoxicity in terms of tissue oxidant/antioxidant parameters and light microscopy in rats. Materials and methods: Wistar albino rats were randomly separated into four groups. Group 1 rats treated with sodium chloride served as the control, group 2 rats were treated with CsA, group 3 with CsA plus erdosteine, and group 4 with erdosteine alone. Animals were killed and blood samples were analyzed for blood urea nitrogen (BUN), serum creatinine (Cr), uric acid (UA), total protein (TP), and albumin (ALB) levels. Kidney sections were analyzed for malondialdehyde (MDA) and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, as well as for histopathological changes. Results: In the CsA group, MDA, GSH-Px, BUN, and Cr levels were increased. The TP and ALB levels were decreased. These changes had been improved by erdosteine administration. Other biochemical parameters did not show any significant change. Conclusion: These results indicate that erdosteine produces a protective mechanism against CsA-induced nephrotoxicity and suggest a role of oxidative stress in pathogenesis.

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Role of Diosmin in protection against the oxidative stress induced damage by gamma-radiation in Wistar albino rats

Regulatory Toxicology and Pharmacology ◽

10.1016/j.yrtph.2020.104622 ◽

2020 ◽

Vol 113 ◽

pp. 104622 ◽

Cited By ~ 3

Author(s):

Shahenda Mahgoub ◽

Anas O. Sallam ◽

Hazem K.A. Sarhan ◽

Amal A.A. Ammar ◽

Sameh H. Soror

Keyword(s):

Oxidative Stress ◽

Gamma Radiation ◽

Albino Rats ◽

Wistar Albino Rats

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Modulation ofN-methyl-d-aspartate receptors in isolated rat heart

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2017-0056 ◽

2017 ◽

Vol 95 (11) ◽

pp. 1327-1334 ◽

Cited By ~ 5

Author(s):

Ivan Srejovic ◽

Vladimir Zivkovic ◽

Tamara Nikolic ◽

Nevena Jeremic ◽

Isidora Stojic ◽

...

Keyword(s):

Oxidative Stress ◽

Rat Heart ◽

Isolated Rat Heart ◽

Albino Rats ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Combined Application ◽

Wistar Albino Rats ◽

Isolated Rat

Considering the limited data on the role of NMDA-Rs in the cardiovascular system, the aim of the present study was to examine the effects of NMDA and DL-Hcy TLHC, alone and in combination with glycine, memantine, and ifenprodil, in the isolated rat heart. The hearts of Wistar albino rats were retrogradely perfused according to the Langendorff technique at a constant perfusion pressure. The experimental protocol for all experimental groups included the stabilization period, application of estimated substance for 5 min, followed by a washout period of 10 min. Using a sensor placed in the left ventricle, we registered the following parameters of myocardial function: dp/dtmax, dp/dtmin, SLVP, DVLP, HR; CF was measured using flowmetry). We estimated the following oxidative stress biomarkers in the coronary venous effluent using spectrophotometry: TBARS, NO2−, O2−, and H2O2. NMDA alone did not induce any change in any of the observed parameters, while DL-Hcy TLHC alone, as well as a combined application of NMDA and DL-Hcy TLHC with glycine, induced a reduction of most cardiodynamic parameters. Memantine and ifenprodil induced a reduction of cardiodynamic parameters and CF, as well as some oxidative stress biomarkers.

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Nanocurcumin Improves the Therapeutic Role of Mesenchymal Stem Cells in Liver Fibrosis Rats

Biointerface Research in Applied Chemistry ◽

10.33263/briac116.1446314479 ◽

2021 ◽

Vol 11 (6) ◽

pp. 14463-14479

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Liver Fibrosis ◽

Therapeutic Agent ◽

Albino Rats ◽

Therapeutic Role ◽

Recovery Group ◽

Wistar Albino Rats ◽

Promising Therapeutic Agent

Nano-curcumin (Nano-Cur) is a promising therapeutic agent that has a wide array of effective medicinal potentials. Therefore, the present inquiry aimed to assess Nano-Cur's impact on the therapeutic effect of bone-marrow-derived mesenchymal stem cells (BM-MSCs) in the rat model of liver fibrosis prompted by carbon tetrachloride (CCl4). Liver fibrosis was developed in 30male Wistar albino rats which were divided into five groups, six animals each. The 1st group (CCl4 group) was sacrificed immediately after the induction of liver fibrosis. The 2nd group received a single iv injection of BM-MSCs and left for 4weeks, the3rd group received 100mg/kg b.w. Nano-Cur 3times/week for 4weeks, the 4th group received a single iv injection of 107 BM-MSCs accompanied with Nano-Cur 3times/week for 4weeks, and the 5th group left for 4weeks without any intervention. Data revealed that treatment with BM-MSCs plus Nano-Cur alleviated liver fibrosis through reducing liver oxidative stress and restoring both liver histological picture and enzymatic profile. Additionally, companied treatment resulted in reducing TGFβ1 levels and attenuating the expression of Smad 2,3 and collagen I, III genes. Conversely, most of the pathological lesions were still detected in the recovery group. Nano-Cur improves the therapeutic role of BM-MSCs in liver fibrosis rats.

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Calcitriol and Erythropoietin Protect Against Cardiac Injury Induced by Renal Ischemia-Reperfusion

Biointerface Research in Applied Chemistry ◽

10.33263/briac106.67186727 ◽

2020 ◽

Vol 10 (6) ◽

pp. 6718-6727 ◽

Cited By ~ 1

Keyword(s):

Morphological Changes ◽

Ischemia Reperfusion ◽

Cardiac Injury ◽

Renal Ischemia ◽

Albino Rats ◽

Cardiac Damage ◽

Beneficial Effects ◽

Tubular Necrosis ◽

Wistar Albino Rats

Cardiac abnormalities and dysfunction are the most important complications after renal ischemia-reperfusion (IR). Thus, investigation and development of effective treatment to decrease cardiac damage induced by renal ischemia are necessary. This study examined the effects of treatment with calcitriol and erythropoietin (EPO) on cardiac injury induced by renal ischemia. Wistar albino rats were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion, followed by 24 h reperfusion. Calcitriol and EPO were administered before ischemia. After 24 h reperfusion, blood samples were collected for the determination of biochemical parameters, and kidney and cardiac samples were taken for histological studies. Renal IR increased BUN-Cr levels, lipid profiles, and myocardial injury markers (CK-MB and LDH). Histopathological findings of the IR group confirmed that there were glomerular atrophy and acute tubular necrosis in the renal tissues and lymphocyte infiltration and intercellular edema in the cardiac samples. Treatment with calcitriol and EPO boosted cardiac and renal functions and improved the morphological changes. It seems that calcitriol or EPO administration could protect against the kidney and cardiac damage induced by IR. Also, the combination of calcitriol and EPO may exert more beneficial effects than either agent used alone.

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