scholarly journals Wnt signaling-mediated redox regulation maintains the germ line stem cell differentiation niche

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Su Wang ◽  
Yuan Gao ◽  
Xiaoqing Song ◽  
Xing Ma ◽  
Xiujuan Zhu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Wnt Signaling ◽  
Redox State ◽  
Germ Line ◽  
Self Renewal ◽  
Cellular Redox ◽  
Germ Line Stem Cell ◽  
And Function ◽  
Cellular Redox State

Adult stem cells continuously undergo self-renewal and generate differentiated cells. In the Drosophila ovary, two separate niches control germ line stem cell (GSC) self-renewal and differentiation processes. Compared to the self-renewing niche, relatively little is known about the maintenance and function of the differentiation niche. In this study, we show that the cellular redox state regulated by Wnt signaling is critical for the maintenance and function of the differentiation niche to promote GSC progeny differentiation. Defective Wnt signaling causes the loss of the differentiation niche and the upregulated BMP signaling in differentiated GSC progeny, thereby disrupting germ cell differentiation. Mechanistically, Wnt signaling controls the expression of multiple glutathione-S-transferase family genes and the cellular redox state. Finally, Wnt2 and Wnt4 function redundantly to maintain active Wnt signaling in the differentiation niche. Therefore, this study has revealed a novel strategy for Wnt signaling in regulating the cellular redox state and maintaining the differentiation niche.

scholarly journals Fly LMBR1/LIMR-type protein Lilipod promotes germ-line stem cell self-renewal by enhancing BMP signaling

2015 ◽  
Vol 112 (45) ◽  
pp. 13928-13933 ◽  
Author(s):  
Darin Dolezal ◽  
Zhiyan Liu ◽  
Qingxiang Zhou ◽  
Francesca Pignoni
Keyword(s):  
Stem Cell ◽  
Limb Development ◽  
Genetic Model ◽  
Germ Line ◽  
Receptor Activation ◽  
Bmp Signaling ◽  
Stem Cell Population ◽  
Self Renewal ◽  
Type Protein ◽  
Germ Line Stem Cell

Limb development membrane protein-1 (LMBR1)/lipocalin-interacting membrane receptor (LIMR)-type proteins are putative nine-transmembrane receptors that are evolutionarily conserved across metazoans. However, their biological function is unknown. Here, we show that the fly family member Lilipod (Lili) is required for germ-line stem cell (GSC) self-renewal in the Drosophila ovary where it enhances bone morphogenetic protein (BMP) signaling. lili mutant GSCs are lost through differentiation, and display reduced levels of the Dpp transducer pMad and precocious activation of the master differentiation factor bam. Conversely, overexpressed Lili induces supernumerary pMad-positive bamP-GFP–negative GSCs. Interestingly, differentiation of lili mutant GSCs is bam-dependent; however, its effect on pMad is not. Thus, although it promotes stem cell self-renewal by repressing a bam-dependent process, Lilipod enhances transduction of the Dpp signal independently of its suppression of differentiation. In addition, because Lili is still required by a ligand-independent BMP receptor, its function likely occurs between receptor activation and pMad phosphorylation within the signaling cascade. This first, to our knowledge, in vivo characterization of a LMBR1/LIMR-type protein in a genetic model reveals an important role in modulating BMP signaling during the asymmetric division of an adult stem cell population and in other BMP signaling contexts.

scholarly journals MILI, a PIWI-interacting RNA-binding Protein, Is Required for Germ Line Stem Cell Self-renewal and Appears to Positively Regulate Translation

2008 ◽  
Vol 284 (10) ◽  
pp. 6507-6519 ◽  
Author(s):  
Yingdee Unhavaithaya ◽  
Yi Hao ◽  
Ergin Beyret ◽  
Hang Yin ◽  
Satomi Kuramochi-Miyagawa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Rna Binding ◽  
Germ Line ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Self Renewal ◽  
Germ Line Stem Cell
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