scholarly journals Hyaluronic acid ameliorates the proliferative ability of human amniotic epithelial cells through activation of TGF-β/BMP signaling

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10104
Author(s):  
Ya-Bing Tian ◽  
Nuo-Xin Wang ◽  
Yan Xu ◽  
Chang-Yin Yu ◽  
Ru-Ming Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Epithelial Cells ◽  
Bmp Signaling ◽  
Inflammatory Factors ◽  
Proliferative Effect ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
Proliferative Ability

Human amniotic epithelial cells (hAECs) are a useful and noncontroversial source of stem cells for cell therapy and regenerative medicine, but their limited proliferative ability hinders the acquisition of adequate quantities of cells for clinical use due to not expressing telomerase in hAECs. Our previous study showed that hyaluronic acid (HA), an important component of the extracellular matrix, promoted the proliferation of human amniotic mesenchymal stem cells. Herein, we hypothesize that HA might improve the proliferative capability of hAECs. In the present study, the role of HA on the proliferation of human amniotic epithelial cells (hAECs) in vitro was investigated for the first time. HA at molecular weight of 300 kDa showed an obvious pro-proliferation effect on hAECs. Furthermore, HA not only kept phenotypic characteristics and differentiation capabilities of hAECs, but significantly promoted the secretion of the anti-inflammatory factors such as IL-10 and TGF-β1, and the expression of stem cell pluripotent factors such as Oct4 and Nanog. Analysis of PCR microarray data and RT-qPCR validation showed that TGF-β/BMP signaling was activated in the presence of HA. Further study showed that SB431542, an inhibitor of the TGF-β/BMP signaling, significantly suppressed the mRNA expression of TGFBR3, BMP4, BMP7, BMPR1B, SMAD3, SMAD4, and the pro-proliferative effect of HA on hAECs. These data suggest that HA is a safe and effective enhancer for in vitro expansion of hAECs, whose regulatory mechanism involves the TGF-β/BMP signaling.

scholarly journals Producing Human Amniotic Epithelial Cells-only Membrane for Transplantation

2019 ◽  
Author(s):  
Chenze Xu ◽  
Waqas Ahmed ◽  
Lili Xie ◽  
Yan Peng ◽  
Qizheng Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Epithelial Cells ◽  
Human Amniotic Membrane ◽  
Transcriptional Level ◽  
Type I ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
Conventional Methods ◽  
Clinical Potential

AbstractHuman amniotic epithelial cells (hAECs), as pluripotent stem cells, have characteristics of immune privilege and great clinical potential. Here, we produced hAECs membrane consisting of single-layer hAECs and basal membrane (BM) of human amniotic membrane (hAM). In conventional methods, hAECs were isolated from hAM by repeated trypsin digestion. In this study, collagenase I and cell scraper were used to remove human amniotic mesenchymal stem cells (hAMSCs) from hAM and hAECs-only membranes were produced. These hAECs on the membranes were evaluated by surface biomarkers including epithelial cell adhesion molecule (EpCAM), stage-specific embryonic antigen 4 (SSEA4) and endoglin (CD105), transcriptional level of biomarkers including POU class 5 homeobox 1 (OCT4), sex determining region Y-box 2 (SOX2), fibroblast growth factor 4 (FGF4), immunofluorescence of cytokeratin-8 (CK-8), alpha smooth muscle actin (α-SMA) and collagen type I alpha 1 chain (ColA1). Finally, the hAECs membrane were transplanted on skin-removed mice to evaluate its effect on wound healing. In comparison to the hAECs isolated by the conventional methods, the cells isolated by this proposed method had higher purity of hAECs, expressed higher in pluripotency related genes, and maintained an epithelium construction in a long-term culture. In mice application, the hAECs membrane effectively improved the skin wound healing. An efficient method was successfully established to produce hAECs membrane in this work which not only held promise to obtain hAECs in higher purity and quality, but also showed practical clinical potential.

scholarly journals Human Amniotic Epithelial Cells Recover Mouse model of Parkinson’s Disease mainly by Neuroprotective，Anti-oxidative and Anti-inflammatory factors

2020 ◽  
Author(s):  
Jiaofei Zhang ◽  
Hui Li ◽  
Hao Yang ◽  
Jianhua Lin ◽  
You Wang ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Epithelial Cells ◽  
Mouse Model ◽  
Inflammatory Factors ◽  
Amniotic Epithelial Cells ◽  
Human Amniotic Epithelial Cells ◽  
Anti Inflammatory

Abstract The authors have withdrawn this preprint due to author disagreement.

scholarly journals Human Amniotic Epithelial Cells Recover Mouse model of Parkinson’s Disease mainly by Neuroprotective，Anti-oxidative and Anti-inflammatory factors

2020 ◽  
Author(s):  
Jiaofei Zhang ◽  
Hui Li ◽  
Hao Yang ◽  
Jianhua Lin ◽  
You Wang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Dopaminergic Neurons ◽  
Microglial Activation ◽  
Inflammatory Factors ◽  
Motor Deficits ◽  
Antibody Array ◽  
Inflammatory Factor ◽  
Antibody Neutralization ◽  
Human Amniotic Epithelial Cells

Abstract Objectives: Human amniotic epithelial cells (hAECs) have been reported to have neuroprotective roles in neurological diseases including Parkinson’s Disease (PD) in animal models. However, the mechanism is not fully understood.Materials and method : Firstly, hAECs were transplanted into the striatum of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine ( MPTP )-induced PD mice. And the motor deficits were tested by Rotarod test. Secondly, injury severity of nigral dopaminergic neurons in substantia nigra and striatal axon fibers in PD mice was estimated by immunochemistry with tyrosine hydroxylase antibody. Thirdly, neuroinflammation was measured by microglial activation and the level of inflammatory factor levels. Lastly, the oxidative stress was tested by the level of reactive oxidative species (ROS) levels. In vitro, we examined a full spectrum of soluble proteins of hAECs by Raybiotech human Antibody Array 507. Besides, antibody neutralization experiments were used to determine the role of many factors on dopaminergic neurons.Results: hAECs significantly attenuated the motor deficits of PD mice. Moreover, the grafts prevented the loss of nigral dopaminergic neurons, promoted the outgrowth of their neurites and striatal axon fibers in PD mice. More importantly, decreased microglial activation, inflammatory factor levels and MPTP-induced excessive ROS levels were also observed in hAEC-treated PD mice. In vitro, analysis of an antibody array of 507 soluble target proteins using hAEC-CM revealed that the levels of many neurotrophic factors, growth factors， neuronal cell adhesion molecule ( NrCAM )，and anti-inflammatory factors were evidently high. In addition, antibody neutralization experiments showed that many of them contributed to the survival and growth of dopaminergic neurons and the outgrowth of their neurites.Conclusion: Our study indicates that the neuroprotection effects by hAECs grafts are achieved not only by neuroprotection and neurite outgrowth-promoting activities, but also by anti-inflammation and anti-oxidation functions in the microenvironment of the damaged dopaminergic neurons, so to facilitate the restoration of neurological functions.

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