Three new species of subterranean amphipods (Pseudocrangonyctidae: Pseudocrangonyx) from limestone caves in South Korea

Pseudocrangonyx Akatsuka & Komai, 1922 is the most diverse group of subterranean amphipods in the groundwater communities of Far East Asia. In Korea, the diversity of the group has been underestimated due to the records of morphological variants of Pseudocrangonyx asiaticus Uéno, 1934. To estimate the species diversity, we analyzed the morphological characteristics and conducted molecular analyses of specimens collected from Korean caves that we treated as morphological variants of P. asiaticus. We described three new subterranean pseudocrangonyctid amphipod species, P. deureunensis sp. nov., P. kwangcheonseonensis sp. nov., and P. hwanseonensis sp. nov., from the groundwater of limestone caves in South Korea. Additionally, we determined sequences of the nuclear large subunit ribosomal RNA and the mitochondrial cytochrome c oxidase subunit I gene of the new species for molecular analyses. Molecular phylogenetic analyses revealed that the three new species formed a monophylum together with P. joolaei Lee et al., 2020 and P. wonkimi Lee, Tomikawa & Min, 2020, which are species that are endemic to Korean caves.

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New Species of Tomentella (Thelephorales, Basidiomycota) from Temperate Continental Mountain Climate of China (Xinjiang Region)

Forests ◽

10.3390/f12111531 ◽

2021 ◽

Vol 12 (11) ◽

pp. 1531

Author(s):

Xu Lu ◽

Haisheng Yuan

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Morphological Characteristics ◽

Northwest China ◽

Tropical Regions ◽

Mountain Climate ◽

Molecular Phylogenetic ◽

Three New Species ◽

Xinjiang Autonomous Region

Species in the genus Tomentella are distributed throughout the temperate and tropical regions worldwide, but few studies associated with the taxonomy and phylogeny of this genus had been reported from Northwest China. In this paper, molecular phylogenetic analyses of the nuclear ribosomal ITS (internal transcribed spacer: ITS1-5.8S-ITS2) and LSU (large subunit: 28S) sequences combined with morphological characteristics identified three new species from Xinjiang Autonomous Region in Northwest China, which were named T. aurantispora, T. kanasensis, and T. schrenkiana. Similar macromorphological and anatomical characteristics are shared by these new species: arachnoid basidiocarps; byssoid sterile margins; utriform basidia with a clamp connection at the base; the absence of rhizomorphs and cystidia; and slightly thick-walled, subglobose to globose basidiospores. Among these new species, the color of the hymenophoral surface, the size of the basidiospores, and some other features can be used for species delimitation. The new species and closely related species in the phylogenetic tree were discussed, and a key to the identified species of Tomentella from China was provided.

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Diversity of Akanthomyces on moths (Lepidoptera) in Thailand

MycoKeys ◽

10.3897/mycokeys.71.55126 ◽

2020 ◽

Vol 71 ◽

pp. 1-22

Author(s):

Arifah Nur Aini ◽

Suchada Mongkolsamrit ◽

Wijanarka Wijanarka ◽

Donnaya Thanakitpipattana ◽

J. Jennifer Luangsa-ard ◽

...

Keyword(s):

New Species ◽

Rna Polymerase ◽

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Pathogenic Fungi ◽

Molecular Data ◽

Three New Species

Akanthomyces is a genus of invertebrate-pathogenic fungi from the family Cordycipitaceae (Ascomycota, Hypocreales). Its species occurs on two different types of hosts, spiders and insects, and in the latter case specifically Lepidoptera adults. Three new species of Akanthomyces, A. noctuidarum, A. pyralidarum, and A. tortricidarum occurring on adult moths from Thailand are proposed based on the differences of their morphological characteristics and molecular data. Phylogenetic analyses using a combined dataset, including the internal transcribed spacer regions, the large subunit of the ribosomal DNA, translation elongation factor 1-α, the largest subunit of RNA polymerase II, and the second largest subunit of RNA polymerase II, support the delimitation of these new species in Akanthomyces.

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Generic classification of some more hyphomycetes with solitary conidia borne on phialides

Canadian Journal of Botany ◽

10.1139/b98-104 ◽

1998 ◽

Vol 76 (9) ◽

pp. 1570-1583 ◽

Cited By ~ 7

Author(s):

W Gams ◽

K O'Donnell ◽

H -J Schroers ◽

M Christensen

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

28S Rdna ◽

New Combination ◽

Rdna Sequences ◽

28S Rdna Sequences ◽

Three New Species ◽

Morphological Species

Unlike most phialide-producing fungi that liberate a multiplicity of conidia from each conidiogenous cell, only single conidia are formed on phialide-like conidiogenous cells in Aphanocladium, Verticimonosporium, and some species of Sibirina. A group of isolates obtained from soil of native Artemisia tridentata (sagebrush) grassland in Wyoming and from desert soil in Iraq is compared with these genera and classified as a fourth genus, Stanjemonium, honouring Stanley J. Hughes. Phylogenetic analyses of partial nuclear small- (18S) and large-subunit (28S) rDNA sequences indicate that Stanjemonium spp. form a monophyletic group with Emericellopsis. Sequences from the nuclear 18S and 28S rDNA were too conserved to resolve morphological species of Stanjemonium; however, phylogenetic analysis of b-tubulin and translation elongation factor 1a gene exons and introns resolved all species distinguished morphologically. Numerous conidiogenous cells or denticles are scattered along the cells of aerial hyphae in Aphanocladium and Stanjemonium spp., very rapidly collapsing into denticles in the former, somewhat more persistent and leaving broad scars in the latter. In Cladobotryum-Sibirina and Verticimonosporium spp., conidiogenous cells are discrete in terminal and intercalary whorls; phialides of the latter taxon are particularly swollen. The taxonomy of Aphanocladium is not yet resolved. Two species are recognized in Verticimonosporium. Three new species of Stanjemonium are described, and one new combination from Aphanocladium is proposed, along with one new species of Cladobotryum.Key words: Aphanocladium, Cladobotryum, conidiogenesis, hyphomycetes, molecular phylogeny, phialide, Stanjemonium, systematics, Verticimonosporium.

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Four new species in the Tremella fibulifera complex (Tremellales, Basidiomycota)

MycoKeys ◽

10.3897/mycokeys.82.63241 ◽

2021 ◽

Vol 82 ◽

pp. 33-56

Author(s):

Long-Fei Fan ◽

Renato Lúcio Mendes Alvarenga ◽

Tatiana Baptista Gibertoni ◽

Fang Wu ◽

Yu-Cheng Dai

Keyword(s):

New Species ◽

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Molecular Data ◽

Small Subunit ◽

Molecular Evidence ◽

Rrna Gene

Samples of species close to Tremella fibulifera from China and Brazil are studied, and T. fibulifera is confirmed as a species complex including nine species. Five known species (T. cheejenii, T. fibulifera s.s., T. “neofibulifera”, T. lloydiae-candidae and T. olens) and four new species (T. australe, T. guangxiensis, T. latispora and T. subfibulifera) in the complex are recognized based on morphological characteristics, molecular evidence, and geographic distribution. Sequences of eight species of the complex were included in the phylogenetic analyses because T. olens lacks molecular data. The phylogenetic analyses were performed by a combined sequence dataset of the internal transcribed spacer (ITS) and the partial nuclear large subunit rDNA (nLSU), and a combined sequence dataset of the ITS, partial nLSU, the small subunit mitochondrial rRNA gene (mtSSU), the translation elongation factor 1-α (TEF1), the largest and second largest subunits of RNA polymerase II (RPB1 and RPB2). The eight species formed eight independent lineages with robust support in phylogenies based on both datasets. Illustrated description of the six species including Tremella fibulifera s.s., T. “neofibulifera” and four new species, and discussions with their related species, are provided. A table of the comparison of the important characteristics of nine species in the T. fibulifera complex and a key to the whitish species in Tremella s.s. are provided.

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Multi-Gene Phylogeny and Taxonomy of Hydnellum (Bankeraceae, Basidiomycota) from China

Journal of Fungi ◽

10.3390/jof7100818 ◽

2021 ◽

Vol 7 (10) ◽

pp. 818

Author(s):

Yan-Hong Mu ◽

Jia-Rui Yu ◽

Ting Cao ◽

Xiang-Hua Wang ◽

Hai-Sheng Yuan

Keyword(s):

New Species ◽

Rna Polymerase Ii ◽

Ribosomal Rna ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Morphological Characteristics ◽

Small Subunit ◽

Phylogenetic Position ◽

Ribosomal Rna Gene ◽

Morphological Studies

The genus Hydnellum is an important group of stipitate hydnaceous fungi which can form ectomycorrhiza with many species of woody plants. In recent decades, the frequency and number of basidiocarps observed in China have been declining significantly. So far, however, we know little about the species diversity of Hydnellum in China. In this study, we conducted molecular phylogenetic analyses based on sections of multiple loci, including the large subunit of nuclear ribosomal RNA gene (nLSU), the internal transcribed spacer regions (ITS), the small subunit of nuclear ribosomal RNA gene (SSU) and the second-largest subunit of RNA polymerase II gene (RPB2), as well as morphological studies, of collected samples of Hydnellum from China. We also inferred Maximum Likelihood and Bayesian phylogenies for the order Thelephorales from the dataset of the combined nLSU and ITS. This study has revealed the phylogenetic position of Hydnellum in the order Thelephorales, and phylogenetically confirmed ten major clades in Thelephorales; Twenty-nine taxa are proposed, described or reported, including 10 new subgenera (Hydnellum subgenus Hydnellum, subg. Caesispinosum, subg. Croceum, subg. Inflatum, subg. Rhizomorphum, subg. Scabrosum, subg. Spongiosum, subg. Subindufibulatum, subg. Violaceum and subg. Zonatum), 11 new species (Hydnellum atrorubrum, H. atrospinosum, H. bomiense, H. brunneorubrum, H. fibulatum, H. granulosum, H. inflatum, H. rubidofuscum, H. squamulosum, H. sulcatum and H. yunnanense), 3 newly recorded species (H. caeruleum, H. peckii and H. spongiosipes) and 5 notable specimens (Hydnellum sp 1, H. sp 2, H. sp 3, H. sp 4 and H. sp 5). A classification system based on the morphological characteristics (especially the hyphal structure types) and molecular analyses is proposed to accommodate most species in Hydnellum. The distinguishing characters of the subgenera and the new species with their closely related taxa are discussed. A key to the species of Hydnellum from China is provided.

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Dothidea kunmingensis, a novel asexual species of Dothideaceae on Jasminum nudiflorum (winter jasmine) from Southwestern China

Phytotaxa ◽

10.11646/phytotaxa.529.1.3 ◽

2021 ◽

Vol 529 (1) ◽

pp. 43-56

Author(s):

YING GAO ◽

JUTAMART MONKAI ◽

ELENI GENTEKAKI ◽

GUANG-CONG REN ◽

DHANUSHKA N. WANASINGHE ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Small Subunit ◽

Morphological Characterization ◽

Small Subunit Rrna ◽

Sister Relationship ◽

Large Subunit Rrna

During a survey of saprobic microfungi in Southwest China, a coelomycetous fungus was found on dead twigs of Jasminum nudiflorum in Kunming, Yunnan Province. Based on a detailed morphological characterization coupled with multi-locus phylogenetic analyses, the fungus was identified as a new species in the genus Dothidea. Phylogenetic analyses using a combined matrix consisting of internal transcribed spacer (ITS), large subunit rRNA (LSU), small subunit rRNA (SSU), beta tubulin (tub2) and translation elongation factor-1 alpha (tef1-α) confirmed its placement in Dothideaceae and revealed a sister relationship to Dothidea eucalypti. The new species is characterized by pycnidial conidiomata, ampulliform or doliiform conidiogenous cells as well as aseptate, subglobose to ovoid, hyaline to pale-brown conidia. Comprehensive descriptions and illustrations are provided. Morphological characteristics of asexual morph taxa in Dothideaceae are also summarized and discussed.

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Three new species of Micropsalliota (Agaricaceae, Agaricales) from China

Phytotaxa ◽

10.11646/phytotaxa.491.2.6 ◽

2021 ◽

Vol 491 (2) ◽

pp. 167-176

Author(s):

JIA-XIN LI ◽

MAO-QIANG HE ◽

RUI-LIN ZHAO

Keyword(s):

New Species ◽

Species Diversity ◽

Phylogenetic Tree ◽

Phylogenetic Analyses ◽

Morphological Characteristics ◽

Southwestern China ◽

Molecular Phylogenetic ◽

Three New Species

Species diversity of Micropsalliota in China remains poorly known, especially in southwestern China, a hotspot of biodiversity. Based on morphological characteristics and molecular phylogenetic analyses using ITS and nrLSU sequences, three new species named Micropsalliota delicatula, M. dentatomarginata and M. digitatocystis are introduced from China. Phylogenetc analyses results indicated the unique phylogenetic positions of three new species in Micropsalliota. Full descriptions, photo plates, illustrations and a phylogenetic tree to show the placement of three new species are presented.

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Morphological characteristics and phylogenetic analyses revealed a new Calocybe (Lyophyllaceae, Basidiomycota) species from northeast China

Phytotaxa ◽

10.11646/phytotaxa.490.2.7 ◽

2021 ◽

Vol 490 (2) ◽

pp. 203-210

Author(s):

JIZE XU ◽

XIAODONG YU ◽

CHUNLAN ZHANG ◽

YU LI

Keyword(s):

New Species ◽

Internal Transcribed Spacer ◽

Northeast China ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Morphological Characteristics ◽

Nuclear Ribosomal Dna ◽

Full Description ◽

Molecular Phylogenetic ◽

A New Species

A new species, Calocybe decurrens, is illustrated and described in detail based on morphological characteristics and phylogenetic analyses. Calocybe decurrens is mainly characterized by its decurrent gills and by its stipe that discolors upon maturation. Molecular phylogenetic analyses were based on the internal transcribed spacer (ITS1-5.8S-ITS2) and the large subunit of the nuclear ribosomal DNA (nrLSU) sequences. The results indicated that its affiliation is in genus Calocybe, where it occupies an isolated position. A full description, color images, illustrations and a phylogenetic tree to show the placement of the new species are provided.

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Unveiling the Hidden Diversity of Rock-Inhabiting Fungi: Chaetothyriales from China

Journal of Fungi ◽

10.3390/jof6040187 ◽

2020 ◽

Vol 6 (4) ◽

pp. 187

Author(s):

Wei Sun ◽

Lei Su ◽

Shun Yang ◽

Jingzu Sun ◽

Bingjie Liu ◽

...

Keyword(s):

New Species ◽

Rna Polymerase ◽

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Morphological Characteristics ◽

Small Subunit ◽

Internal Transcribed Spacer Region ◽

Natural Rock ◽

Rock Inhabiting Fungi

Rock-inhabiting fungi (RIF) are nonlichenized fungi that naturally colonize rock surfaces and subsurfaces. The extremely slow growth rate and lack of distinguishing morphological characteristics of RIF resulted in a poor understanding on their biodiversity. In this study, we surveyed RIF colonizing historical stone monuments and natural rock formations from throughout China. Among over 1000 isolates, after preliminary delimitation using the internal transcribed spacer region (ITS) sequences, representative isolates belonging to Trichomeriaceae and Herpotrichiellaceae were selected for a combined analysis of ITS and the nuclear ribosomal large subunit (nucLSU) to determine the generic placements. Eight clades representing seven known genera and one new genus herein named as Anthracina were placed in Trichomeriaceae. While, for Herpotrichiellaceae, two clades corresponded to two genera: Cladophialophora and Exophiala. Fine-scale phylogenetic analyses using combined sequences of the partial actin gene (ACT), ITS, mitochondrial small subunit ribosomal DNA (mtSSU), nucLSU, the largest subunit of RNA polymerase II (RPB1), small subunit of nuclear ribosomal RNA gene (SSU), translation elongation factor (TEF), and β-tubulin gene (TUB) revealed that these strains represented 11 and 6 new species, respectively, in Trichomeriaceae and Herpotrichiellaceae. The 17 new species were described, illustrated for their morphologies and compared with similar taxa. Our study demonstrated that the diversity of RIF is surprisingly high and still poorly understood. In addition, a rapid strategy for classifying RIF was proposed to determine the generic and familial placements through preliminary ITS and nucLSU analyses, followed by combined analyses of five loci selected from ACT, ITS, mtSSU, nucLSU, RPB1, and/or the second subunit of RNA polymerase II gene (RPB2), SSU, TEF, and TUB regions to classify RIF to the species level.

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Heterobasidion amyloideopsis sp. nov. (Basidiomycota, Russulales) evidenced by morphological characteristics and phylogenetic analysis

Phytotaxa ◽

10.11646/phytotaxa.317.3.4 ◽

2017 ◽

Vol 317 (3) ◽

pp. 199 ◽

Cited By ~ 2

Author(s):

CHANG-LIN ZHAO ◽

MALKA SABA ◽

ABDUL NASIR KHALID ◽

JIE SONG ◽

DONALD H. PFISTER

Keyword(s):

New Species ◽

Maximum Likelihood ◽

Rna Polymerase ◽

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Morphological Characteristics ◽

Molecular Evidence ◽

Gene Encoding ◽

Annual Habit

Heterobasidion amyloideopsis sp. nov., a new poroid wood-inhabiting species from Pakistan, is introduced based on a combination of molecular evidence and morphological characteristics. We generated sequences from the nuclear internal transcribed spacer regions (ITS) and the large subunit ribosomal RNA gene (LSU), the gene encoding the largest subunit of RNA polymerase II (RPB1) and the second subunit of RNA polymerase II (RPB2), focusing on two specimens from Pakistan. We performed phylogenetic analyses with maximum likelihood, maximum parsimony, and bayesian inference methods on two datasets (RPB1+RPB2 and ITS+nLSU+RPB1+RPB2). Both analyses supported the existence of the new species and showed that it formed a monophyletic group within the H. insulare complex as a sister to H. amyloideum. In addition to assessing the origin and divergence of this new species, we focused on the RPB1+RPB2 dataset to perform maximum likelihood based estimation and Bayesian binary analyses. Heterobasidion amyloideopsis is characterized by an annual habit, pileate basidiomata with a rust colored pileal surface, white, obtuse margin, a dimitic hyphal system with simple septate generative hyphae in the trama and clamp connections present on the contextual hyphae, amyloid skeletal hyphae and broadly ellipsoid, hyaline, fairly thick-walled, and asperulate basidiospores.

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