scholarly journals Iberian pig adaptation to acorn consumption: I. Net portal appearance of metabolites

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5861 ◽  
Author(s):  
Ignacio Fernández-Fígares ◽  
Jose Miguel Rodríguez-López ◽  
Lucrecia González-Valero ◽  
Manuel Lachica
Keyword(s):  
Sampling Period ◽  
Metabolic Energy ◽  
Blood Collection ◽  
Standard Diet ◽  
Deficient Diet ◽  
Protein Energy ◽  
Sampling Session ◽  
Protein Deficient Diet ◽  
Portal Plasma ◽  
Set Up

Most valuable cured products from Iberian pigs come from pure bred animals raised for a final grazing-fattening period where pigs eat mainly acorns, a low protein energy rich fruit. This is a nutritional challenge for animals fed equilibrated diets from weaning. The aim of the study was to determine net portal appearance (NPA) of metabolites in gilts fed acorns and evaluate adaptational changes after one week of feeding. Two sampling periods were carried out (after one day and after one week of acorn feeding) with six gilts (34 kg average BW) set up with three catheters: in carotid artery and portal vein for blood sampling, and ileal vein for para-aminohippuric acid (PAH) infusion to measure portal plasma flow (PPF). Pigs were fed at 2.5 × ME for maintenance a standard diet in two portions, at 09:00 (0.25) and 15:00 h (the remaining 0.75). On the day prior to the first sampling period, pigs were fed 2.4 kg of oak acorns. After feeding 0.25 of ration a 6 h serial blood collection was initiated. Following an identical protocol, a second sampling session was performed 1 week later. Adaptation to acorn consumption decreased NPA of ammonia (47%,P < 0.001). Although there was a transfer of urea from the gastrointestinal tract to the circulation in both sampling periods, no differences in NPA of urea was found (P > 0.05). NPA of glucose was not influenced by sampling period (P > 0.05), but NPA of lactate was greatly increased (231%,P < 0.001). There was a negative NPA of albumin although adaptation to acorn feeding did not alter it. Although NPA of triglycerides and cholesterol were unchanged, a subtle increase in arterial and portal cholesterol was noticed (9.6%,P < 0.01). Pigs fed a protein deficient diet for one week adapted decreasing NPA of ammonia for saving metabolic energy as less ammonia would become available for conversion to urea.

scholarly journals Iberian pig adaptation to acorn consumption: II. Net portal appearance of amino acids

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e6137 ◽  
Author(s):  
Manuel Lachica ◽  
Jose Miguel Rodríguez-López ◽  
Lucrecia González-Valero ◽  
Ignacio Fernández-Fígares
Keyword(s):  
Blood Collection ◽  
Standard Diet ◽  
Peripheral Tissues ◽  
Oak Trees ◽  
Portal Plasma ◽  
Iberian Pig ◽  
Grazing Period ◽  
Limiting Amino Acid ◽  
Set Up ◽  
Fractional Absorption

In Iberian pig outdoor production, pigs are fed equilibrated diets until the final fattening period when grazing pigs consume mainly acorns from oak trees. Acorns are rich in energy but poor in crude protein where lysine is the first limiting amino acid (AA). Net portal appearance (NPA) is very useful to ascertain AA available for liver and peripheral tissues. The aim of this study was to determine NPA of AA in Iberian gilts fed with acorns and to ascertain if there was an effect of acorn feeding over time. Two sampling periods were carried out (after one day and after one week of acorn feeding) with six gilts (34 kg average BW) set up with three catheters: in carotid artery and portal vein for blood sampling, and ileal vein for a marker infusion to measure portal plasma flow (PPF). Pigs were fed at 2.5 × ME for maintenance a standard diet in two meals, at 09:00 (0.25) and 15:00 h (the remaining 0.75). The day previous to first sampling, pig diet was replaced by 2.4 kg of acorn. A serial blood collection was done at −5 min, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5 and 6 h after feeding 0.25 of total daily acorn ration. Following identical protocol, one week later the second sampling was done. NPA of sum of essential AA (EAA) was poor. Although increased NPA of histidine (P < 0.001), leucine, phenylalanine and valine (0.05 < P < 0.08) was found after one week of acorn consumption, the sum of EAA did not change. Furthermore, fractional absorption (NPA/AA intake) of EAA, non-essential AA (NEAA) and total AA was 97, 44 and 49% lower, respectively, at the beginning of eating acorn than a week later. Supplementation, with some of the EAA and NEAA to Iberian pigs during the grazing period would be beneficial to overcome the increased portal-drained viscera (PDV) utilization of AA observed in the present study.

scholarly journals Effects of supplemental protein and energy and feeding frequency on the performance of lactating dairy cows fed a protein deficient diet

2002 ◽  
Vol 82 (2) ◽  
pp. 225-231 ◽  
Author(s):  
M. C. Thivierge ◽  
J. F. Bernier ◽  
H. Lapierre
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Protein ◽  
Basal Diet ◽  
Protein Yield ◽  
Meal Frequency ◽  
Feeding Frequency ◽  
Deficient Diet ◽  
Protein Energy ◽  
Protein Deficient Diet

The objective of this experiment was to study the interaction between feeding frequency and supplementation of a protein-deficient diet with an undegradable protein and energy source on milk production and composition. Eight Holstein cows were used in a 2 × 2 factorial arrangement, using a replicated 4 × 4 Latin square design with 14-d experimental periods. The main factors were feeding frequency of the basal diet (two vs. seven times daily) and the addition of a protein-energy supplement fed in seven meals daily. Intake of the basal diet was individually restricted to 95% of previous ad libitum intake. The supplement consisted of 1.71 kg of Pro-Lak® (70% rumen-undegradable protein) and 0.94 kg of dried molasses. The basal diet provided 69, 89, 54, and 95% of the daily requirements of crude protein, rumen-degradable and rumen-undegradable proteins and NEL, respectively. Milk protein yield increased (P = 0.06) by 4% with increased feeding frequency. Milk and milk protein yield both increased (P < 0.01) by 12% with the supplement, but no interaction between feeding frequency and the supplement was observed. Urinary excretion of purine derivatives, an index of rumen microbial protein synthesis, was not affected by treatments. Thus, the results indicate that microbial growth was not affected by meal frequency or the protein-energy supplement, maybe due to the high supply of rumen-degradable protein from the basal diet. The effect of increasing meal frequency on milk protein yield might be related to an increased post-ruminal digestibility of organic matter. Key words: Dairy cows, feeding frequency, milk production, protein, purines

scholarly journals Using a simple rope-pulley system that mechanically couples the arms, legs, and treadmill reduces the metabolic cost of walking

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Daisey Vega ◽  
Christopher J. Arellano
Keyword(s):  
Metabolic Cost ◽  
Metabolic Energy ◽  
Metabolic Effects ◽  
Whole Body ◽  
Walking Ability ◽  
Arm Swing ◽  
Pulley System ◽  
Young Subjects ◽  
Walking Recovery ◽  
Set Up

Abstract Background Emphasizing the active use of the arms and coordinating them with the stepping motion of the legs may promote walking recovery in patients with impaired lower limb function. Yet, most approaches use seated devices to allow coupled arm and leg movements. To provide an option during treadmill walking, we designed a rope-pulley system that physically links the arms and legs. This arm-leg pulley system was grounded to the floor and made of commercially available slotted square tubing, solid strut channels, and low-friction pulleys that allowed us to use a rope to connect the subject’s wrist to the ipsilateral foot. This set-up was based on our idea that during walking the arm could generate an assistive force during arm swing retraction and, therefore, aid in leg swing. Methods To test this idea, we compared the mechanical, muscular, and metabolic effects between normal walking and walking with the arm-leg pulley system. We measured rope and ground reaction forces, electromyographic signals of key arm and leg muscles, and rates of metabolic energy consumption while healthy, young subjects walked at 1.25 m/s on a dual-belt instrumented treadmill (n = 8). Results With our arm-leg pulley system, we found that an assistive force could be generated, reaching peak values of 7% body weight on average. Contrary to our expectation, the force mainly coincided with the propulsive phase of walking and not leg swing. Our findings suggest that subjects actively used their arms to harness the energy from the moving treadmill belt, which helped to propel the whole body via the arm-leg rope linkage. This effectively decreased the muscular and mechanical demands placed on the legs, reducing the propulsive impulse by 43% (p < 0.001), which led to a 17% net reduction in the metabolic power required for walking (p = 0.001). Conclusions These findings provide the biomechanical and energetic basis for how we might reimagine the use of the arms in gait rehabilitation, opening the opportunity to explore if such a method could help patients regain their walking ability. Trial registration: Study registered on 09/29/2018 in ClinicalTrials.gov (ID—NCT03689647).

scholarly journals Veterinary needs of dogs in two resource-limited communities in the Gauteng and North West provinces of South Africa

2001 ◽  
Vol 72 (4) ◽  
Author(s):  
W.N. Minnaar ◽  
R.C. Krecek
Keyword(s):  
South Africa ◽  
Health Status ◽  
Body Condition ◽  
Veterinary Services ◽  
Structured Interviews ◽  
Deficient Diet ◽  
North West ◽  
The North ◽  
Resource Limited ◽  
Protein Deficient Diet

Information on the socioeconomic aspects and the health status of dogs in 2 resource-limited communities in the North West and Gauteng provinces of South Africa was gathered using semi-structured interviews and a standardised questionnaire. The dogs were examined clinically to determine their health status, and their body condition and age were scored. Most of the dogs (93 % in Jericho and 90 % in Zuurbekom) were infected with hookworm, which poses a threat to animal and human health in the 2 study areas. Many dogs were also being given a protein-deficient diet, which together with hookworm parasites would impact considerably on the dog's health. Dogs were mainly kept for security reasons. The need indicated to be most important by the residents of the 2 commnities was a lack of available and affordable veterinary services.

scholarly journals The effects of a tryptophan- and protein-deficient diet upon growth in rats

1989 ◽  
Vol 27 (4) ◽  
pp. 345-347 ◽  
Author(s):  
Angela H. Becker ◽  
Stephen F. Davis ◽  
Cathy A. Grover ◽  
Cynthia A. Erickson
Keyword(s):  
Deficient Diet ◽  
Protein Deficient Diet

scholarly journals How Much Energy Vaquejada Horses Spend in a Field Simulation Test?

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3421
Author(s):  
Clarisse S. Coelho ◽  
Ticiane D. R. P. Sodre ◽  
Lara N. Sousa ◽  
Renata F. Siqueira ◽  
Helio C. Manso Filho ◽  
...  
Keyword(s):  
Well Being ◽  
Metabolic Energy ◽  
Energy Requirements ◽  
Transport Costs ◽  
Simulation Test ◽  
Blood Samples ◽  
Energy Expenditures ◽  
Field Simulation ◽  
Set Up ◽  
One Way Anova

Vaquejada is a high-intensity and short-duration exercise in which helper horses (HH) are responsible to keep a bull running in a line while pull horses (PH) work to put the bull down after 100 m of running. The purpose of this study was to quantify and compare energy expenditures (EE), transport costs (COT) and metabolic energy requirements (Pmet) of horses used in Vaquejada. Thus, eight Quarter Horses, in randomly formed pairs, performed a vaquejada simulation test (VST), which consisted of three races on a sand track (130–150 m), with a 5-min interval between them. All horses used an integrated heart rate (HR) and GPS monitoring system (V800, Polar Electro) and, from these data, EE, COT and Pmet were calculated using the formulas: EE (J/kg/min) = 0.0566 × HR1.9955, COT = (HR-35)/kg/m × 103 and Pmet = (HR-35)/min/kg. Blood samples were collected for lactate analysis at rest, immediately after the first, second and third race and after 30 min of recovery. Data obtained were submitted to one-way ANOVA and Tukey tests (p ≤ 0.05). In VST, HH had higher EE and higher HR at trot; while PH presented higher EE and HR at canter. Finally, considering total VST, PH had higher EE and COT, while HH had higher Pmet. Lactate was higher in PH. Despite practicing the same sport, PH and HH should be considered distinct athletes, and these must be considered to set up appropriate physical and nutritional programs, which will lead to better performance and guarantees of well-being.

scholarly journals Drifting Behaviour of Aquatic Mites and Regulating Ecological Parameters in Khankragad Stream, A Springfed Trubutary of Alaknanda River, Rudrarayag, Garhwal, Uttarakhand, India

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Shailza Negi ◽  
Pankaj Bahuguna ◽  
Anoop Dobriyal
Keyword(s):  
Water Quality ◽  
Environmental Changes ◽  
Sampling Period ◽  
Mite Species ◽  
Water Mites ◽  
Water Mite ◽  
Physico Chemical ◽  
Ecological Parameters ◽  
Drift Behavior ◽  
Set Up

Water mites are a large group of macroinvertebrates that are very vulnerable to contamination and environmental changes. As a result, they are often used to monitor water quality. Khankra gad stream, a third-order perennial spring-fed stream of the Alaknanda River in Uttarakhand, India, was studied to determine the drift behavior of water mites and water quality. Drift nets were set up for 24 hours in both spots of the Khankra stream where there was little or no human influence over a two-year sampling period (June 2018-20). Every four hours, the nets were changed out with new ones. Water mites signify a particular drift month and diel drift pattern. The majority of the drift mite species were present in considerably higher numbers in the daytime. During the two-year study period, a total of 2503 mite samples were collected from the Khankra stream, from which 204 water mite species drifted. The least number of Hydrachnidia (694) were collected from Spot-1, and the highest (1809) were collected from Spot-2, with 106 water mites drifting from Spot-1 and 134 mites drifting from Spot-2 during the study period. From Spot-2 maximum 25 mite species were collected and minimum 19 mite species were collected from Spot-1. For various mite species, the DBDI value ranged from 0.155 (July) to 0.204 (April) in Spot-1 and 0.134 (July) to 0.149 (February) in Spot-2. It was also observed that maximum water mite species were day-drifter. The physico-chemical parameters of the Khankra stream were also reported. The effect of ecological parameters on mite drift was investigated using Canonical correspondence analysis (CCA).

LIPID METABOLISM AND LIPOPROTEIN SUSCEPTIBILITY TO PEROXIDATION ARE AFFECTED BY A PROTEIN-DEFICIENT DIET IN THE RAT

1997 ◽  
Vol 17 (1) ◽  
pp. 125-135 ◽  
Author(s):  
Corinne Moundras ◽  
Christian Demigné ◽  
Christine Morand ◽  
Marie-Anne Levrat ◽  
Christian Rémésy
Keyword(s):  
Lipid Metabolism ◽  
Deficient Diet ◽  
Protein Deficient Diet

Time-Delayed Sampled-Data Feedback Control of Differential Systems Undergoing Hopf Bifurcation

2021 ◽  
Vol 31 (01) ◽  
pp. 2150004
Author(s):  
Huan Su ◽  
Jing Xu
Keyword(s):  
Hopf Bifurcation ◽  
Feedback Control ◽  
Blood Transfusion ◽  
Sampling Period ◽  
Delayed Feedback Control ◽  
Control Technique ◽  
Blood Collection ◽  
Sampled Data ◽  
Differential Systems ◽  
Data Feedback

In this paper, time-delayed sampled-data feedback control technique is used to asymptotically stabilize a class of unstable delayed differential systems. Through the analysis for the distribution change of eigenvalues, an effective interval of the control parameter is obtained for a given sampling period. Here an indirect strategy is taken. Specifically, the system of continuous-time delayed feedback control is studied first by Hopf bifurcation theory. And then, the result and implicit function theorem are used to analyze the system of time-delayed sampled-data feedback control with a sufficiently small sampling period. Considering the practical criterion for the size of sampling period, the upper bound of sampling period is estimated. Finally, an application example, an unstable Mackey–Glass model, is asymptotically stabilized by introducing a blood transfusion item with time-delayed sampled-data feedback control. The blood transfusion speed and blood collection test period are derived from the main results. Some simulations and comparisons show the correctness and advantages of the main theoretical results.

scholarly journals INFLUENCE OF DIETARY PROTEIN CONTENT ON Trypanosoma cruzi INFECTION IN GERMFREE AND CONVENTIONAL MICE

1998 ◽  
Vol 40 (6) ◽  
pp. 355-362 ◽  
Author(s):  
Isa P. CINTRA ◽  
Marcelo E. SILVA ◽  
Marcílio E.C. SILVA ◽  
Márcio E. SILVA ◽  
L.C. C. AFONSO ◽  
...  
Keyword(s):  
Weight Gain ◽  
Trypanosoma Cruzi ◽  
Dietary Protein ◽  
Blood Cell Count ◽  
Deficient Diet ◽  
Low Protein ◽  
Protein Deficient Diet ◽  
Red Blood Cell Count ◽  
Cruzi Infection ◽  
Microbiological Status

Germfree (GF) and conventional (CV) mice were fed on diets containing 4.4, 13.2 or 26.4% of protein (weight/weight). CV mice fed on low protein diet did not gain weight during four weeks, whereas the protein deficient diet did not affect the growth of GF mice. After four weeks on these diets, the mice were inoculated with 5x103 trypomastigotes of Trypanosoma cruzi. The protein deficiency affected less the GF than the CV mice, according to the following parameters: weight gain, hemoglobin, plasma protein and albumin levels and water and protein contents of the carcass. Infection with T. cruzi produced a significant decrease in hemoglobin levels, red blood cell count, and water and protein contents in the carcass. This decrease was more pronounced in the GF mice. Histopathologically, there was no difference between the treatments in animals with the same microbiological status (GF or CV). However, the disease was more severe in the GF than in the CV mice.

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